A Late‐glacial chironomid record from Hawes Water,northwest England

This paper presents the results of a high‐resolution Late‐glacial chironomid stratigraphy from Hawes Water, a small carbonate lake in northern Lancashire. The samples were from a core taken from the terrestrialised margin of the present lake, which represents an intermediate depth between the true littoral and the profundal. The chironomid assemblage showed a high degree of sensitivity to both broad‐scale and short‐term temperature changes. Comparison with an existing proxy temperature record (δ¹⁸O) for the site confirmed the presence of four temperature inversions within the Late‐glacial Interstadial. A mean July air temperature inference model, derived from acid, soft‐water lakes in Norway and Svalbard, was applied to the data. Despite the absence of carbonate lakes within the Norwegian training set, there was a close similarity between trends in estimated July air temperature and the δ¹⁸O trace, with a particularly strong correspondence in the periods of clay deposition. This suggests that this model is highly robust. The inferred maximum Interstadial temperature was 13.4°C, dropping initially to 7.5°C in the Loch Lomond Stadial. Temperatures reach a maximum of nearly 10°C in this period, cool for a short period before rising rapidly to 13.2°C at the start of the Holocene. These temperatures are similar to but slightly higher than those estimated for Whitrig Bog, southeast Scotland, and lower than those inferred from coleopteran‐based models for sites in South Wales. Copyright © 2004 John Wiley & Sons, Ltd.     

西沙海域天然气水合物地震资料高密度双谱速度分析技术探讨

基于南海西沙海域天然气水合物二维高分辨率多道地震数据资料,使用Geovation地震资料处理系统的高密度双谱速度分析方法进行处理。在叠前时间偏移处理后的道集基础上,进行高密度双谱非双曲线型NMO自动拾取,最终获得解决各向异性问题后的叠前时间偏移剖面。分析结果表明,高密度双谱速度分析技术实现了速度逐道逐点的自动拾取,提高了时间方向的分辨能力、空间方向的分析密度,可以充分挖掘水合物地震数据的潜力。高密度双谱速度分析方法在提高处理质量的同时,可以帮助判定水合物的富集层位,研究似海底反射层附近的详细速度结构,为西沙海域水合物地震资料综合解释提供依据。     

水杨酸对蛋白核小球藻(Chlorella pyrenoidosa) 生长及抗逆相关基因的影响

以蛋白核小球藻(Chlorella pyrenoidosa)为研究对象, 首先克隆了热激蛋白70(HSP70)和磷 酸甘油酸激酶(PGK)基因全长序列, 然后研究了0-20μg/mL 水杨酸对高温胁迫下HSP70 和PGK 基 因表达, 以及蛋白核小球藻生长、可溶性糖和蛋白含量的影响。结果获得了蛋白核小球藻HSP70 基 因的cDNA 序列2405 bp, 包括60 bp 的5'-非编码区(UTR)、1959 bp 的开放阅读框(ORF)和386 bp 的3'-UTR 和PGK 基因的cDNA 序列1664 bp, 包括35 bp 的5'-UTR, 1398 bp ORF 和231 bp 3'-UTR. 序列比较和分析表明该蛋白核小球藻HSP70 序列与其它绿藻的同源性高达83%-91%, PGK 为 70%-75%.荧光定量PCR 结果显示随着水杨酸浓度的增加, HSP70 和PGK 的表达量在12h 和24h 均有所增加, 而在10 μg/mL 水杨酸浓度下表达量最大, 12h 时分别为对照组的2.57 倍和1.56 倍, 24h 则为1.71 倍和1.79 倍。1-20μg/mL 水杨酸可促进该藻的比生长速率、可溶性糖和蛋白含量的升高。 本文结果表明一定浓度水杨酸对高温胁迫蛋白核小球藻有缓解作用, 且5 和10 μg/mL 水杨酸的效果 最显著。     

Genetic structure of the tall sea pen Funiculina quadrangularis in NW Scottish sea lochs

The tall sea pen Funiculina quadrangularis has a patchy distribution around the UK, being found in sheltered waters below 20 m depth on the northwest coast of Scotland and Ireland. The limited distribution and sensitivity to bottom fishing activities make F. quadrangularis vulnerable to reduction in population numbers that may lead to genetic isolation and reduced diversity. Because of this vulnerability and low resilience to physical disturbance, the tall sea pen is recognised as a Species of Principle Importance under the Natural Environment and Rural Communities Act, 2006, UK. ( http://www.legislation.gov.uk/ukpga/2006/16/contents ) and is also on the Biodiversity Action Plan list of Priority Species for the UK. In the Mediterranean it is recognised as a sensitive and essential fish habitat because it forms habitat for several commercially important crustaceans. The aim of this study was to understand the current state of the genetic structure and gene flow of F. quadrangularis in areas of NW Scotland. We developed 10 microsatellite markers and used them to genotype 176 samples from four populations. Overall, our results suggest that there is high genetic diversity and high gene flow between colonies of F. quadrangularis in and among locations in Loch Linnhe and Loch Duich. As a result of the high rates of gene flow, genetic differentiation between sites was low. This may provide resilience to human impacts if distant populations have a high connectivity. However, care must be taken, as small but significant isolation by distance was found between the most geographically distant sites and only a small part of the species range was examined in this study. The genetic tools developed here will provide a foundation for wider studies of this vulnerable species.     

Shell‐shape and morphometric variability in Mytilus galloprovincialis from micro‐tidal environments: responses to different hydrodynamic drivers

Tidal conditions differently influence inter‐tidal organisms in terms of general physiological and metabolic responses. In this study we investigated the morphological response in shells of Mytilus galloprovincialis native to different micro‐tidal coastal environments in the Northern Adriatic Sea. Our purpose was to highlight the ecophenotypic variability across tidal levels and to elucidate how tidal currents and waves produced by anthropogenic activities may play a part in modulating shell morphology. Three sampling sites were selected: an open‐sea area 15 km off‐shore and two sites within the lagoon of Venice, the first near one of its three inlets, and the other one in the proximity of Venice city centre. At each sampling site, organisms were seasonally collected at different depths within their vertical zonation, either in the inter‐tidal zone – i.e. at both the highest and lowest tide zonation limits, and subtidally. The mussel shells were analysed by investigation of their morphometric relationships (height/length and width/length ratios) and by elliptic Fourier analysis of the shell contours. Shell thickness and condition index were also evaluated for a better comprehension of energy allocation/partitioning. Estimates based on long‐term measurements, visual observation, wind statistics and wave growth laws allowed an evaluation of the forces acting on shells. At the open‐sea site, the observed phenotypic variability of both shell shape and thickness was clearly related to the tidal vertical zonation. At the two lagoon sites, the currents generated by tidal flow through the inlet and the waves caused by the frequent passage of boats influenced both shell shape and thickness. A trade‐off between protection and growth was apparent along the tide gradient, as emphasized by the differences in the partitioning and allocation of energy between shell and flesh production.     

Concurrent imaging of chlorophyll fluorescence,Chlorophyll a content and green fluorescent proteins‐like proteins of symbiotic cnidarians

Research on photosynthetic cnidarians has been mainly focused on the symbiosis established between the cnidarian host and its dinoflagellates endosymbionts from genus Symbiodinium. Despite the potential of imaging techniques for assessing the spatial distribution of key parameters of cnidarian photobiology, such as photochemical activity, chlorophyll a content or green fluorescent proteins (GFPs), to our best knowledge, no study has ever attempted to simultaneous map these three features. In this study, we developed a modified imaging pulse amplitude fluorometer by applying excitation light of different wavelengths and selectively detecting short spectral bands through bandpass filters. The imaging system was used to sequentially excite and quantify chlorophyll variable fluorescence (maximum quantum yield of photosystem II, F_v/F_m), Chl a content (normalized difference vegetation index) and relative content of GFPs. The spatial distribution of these photophysiological parameters was mapped both horizontally, across the surface of the soft corals Sarcophyton cf. glaucum and Sinularia flexibilis and the zoanthid Protopalythoa sp., and vertically, throughout a vertical section of S. cf. glaucum. Results showed bleached areas within each individual coral colony and registered photophysiological changes with S. cf. glaucum tissue depth. Analysis of Protopalythoa sp. polyps’ expansion revealed differential surface patterns of NDVI and GFP concentration, and a negative relation between these latter parameters within each polyp. This novel non‐invasive approach allowed a high‐resolution characterization of the spatial relationship between these key parameters through the analysis of image information on a pixel‐by‐pixel basis, which has great potential for investigating the physiological state of symbiotic associations.     

便携式高频地波雷达台湾海峡浪高观测

As an important equipment for sea state remote sensing, high frequency surface wave radar(HFSWR) has received more and more attention. The conventional method for wave height inversion is based on the ratio of the integration of the second-order spectral continuum to that of the first-order region, where the strong external noise and the incorrect delineation of the first- and second-order Doppler spectral regions due to spectral aliasing are two major sources of errors in the wave height. To account for these factors, two more indices are introduced to the wave height estimation, i.e., the ratio of the maximum power of the second-order continuum to that of the Bragg spectral region(RSCB) and the ratio of the power of the second harmonic peak to that of the Bragg peak(RSHB). Both indices also have a strong correlation with the underlying wave height. On the basis of all these indices an empirical model is proposed to estimate the wave height. This method has been used in a three-months long experiment of the ocean state measuring and analyzing radar, type S(OSMAR-S), which is a portable HFSWR with compact cross-loop/monopole receive antennas developed by Wuhan University since 2006. During the experiment in the Taiwan Strait, the significant wave height varied from 0 to 5 m. The significant wave heights estimated by the OSMAR-S correlate well with the data provided by the Oceanweather Inc. for comparison, with a correlation coefficient of 0.74 and a root mean square error(RMSE) of 0.77 m. The proposed method has made an effective improvement to the wave height estimation and thus a further step toward operational use of the OSMAR-S in the wave height extraction.     

利用不同盐度诱导长牡蛎三倍体的研究

采用低盐度(4、6、8、10、12、14、16)和高盐度(40、45、50、55、60、65、70)在40%~50%受精卵出现第一极体时处理10和15min,进行长牡蛎三倍体的诱导。在低盐度诱导三倍体实验中,随着盐度降低,D型幼虫孵化率逐渐降低,处理10和15min的卵裂率差异显著(P0.05),孵化率差异不显著(P0.05)。当盐度为8时,处理受精卵10与15min,三倍体诱导率最高分别可达84.4%和91.0%,两处理组三倍体诱导率差异显著(P0.05)。在高盐度诱导三倍体实验中,随着盐度升高,D型幼虫孵化率逐渐降低,处理10和15min的卵裂率和孵化率均差异显著(P0.05)。当盐度为55时,处理受精卵10min,三倍体诱导率最高为80.3%;当盐度为60时,处理受精卵15min,三倍体诱导率最高为97.1%,两处理组三倍体诱导率差异显著(P0.05)。研究发现,在盐度为8和60条件下,40%~50%的受精卵出现第一极体时,处理15min,长牡蛎三倍体诱导率最高。     

高桩挡板透空式防波堤消浪性能数值研究

以RANS方程为控制方程,基于有限体积法,在动量方程中添加源项,建立了具有造波‐消波功能的数值波浪水槽。利用建立的源项造波数值波浪水槽,模拟了高桩挡板透空式防波堤在规则波作用下的消浪效果,完整地再现了堤前堤后的流态,分析了挡板相对入水深度对透浪系数的影响。在与试验值及拉帕公式对比后发现,数模计算结果与试验值较接近,拉帕公式偏大。研究了堤顶相对宽度、相对水深、相对波高对透浪系数的影响并提出修正公式,修正公式与计算值和试验值吻合较好。