The bivalve Lembulus bicuspidatus may enhance denitrification in shelf sediment at the Angola–Benguela Frontal Zone

We collected living individuals of the bivalve Lembulus bicuspidatus, which shows an unusual preference for the oxygen-deficient habitat found at the Angola–Benguela Frontal Zone of the southeastern Atlantic. With a series of incubation experiments with ¹⁵N-labelled nitrate as a tracer in combination with membrane-inlet mass spectrometry, we studied the potential contribution of L. bicuspidatus to nitrate reduction in the upper sediment layer. Our preliminary results suggest that L. bicuspidatus enhances nitrate reduction if the oxygen concentration is sufficiently low. The Lembulus-mediated nitrate reduction rate is then similar to the rate of microbial nitrate reduction in the surrounding sediment.     

发现于我国渤海辽东湾的海洋甲藻新种-渤海异冒藻(Heterocapsa bohaiensis sp. nov.)

A small armed dinoflagellate bloomed in the aquaculture ponds off the coast of Liaodong Bay, Bohai Sea of China,resulting in heavy mortalities of the cultured prawns(Penaeus japonicus) and larvae of Chinese mitten handed crabs(Eriocheir sinensis). The bloom-forming species was successfully isolated, and cellular morphology of the specimen was consequently investigated through light, fluorescent and electron microscopy. The small((14.4±1.6) μm in length) ellipsoid cells show typical Heterocapsa thecal plate arrangement(Po, cp, 5′, 3 a, 7′′, 6 c,5 s, 5′′′, 2′′′′). The episome is evidently bigger than the hyposome. One to three spherical pyrenoids are located above or beside the large elongated nucleus. The body scale is characterized by a triangle basal plate with one central upright and nine peripheral spines. Above all, Heterocapsa bohaiensis could be distinguished from other Heterocapsa species by the combination of the cell size, morphology, cellular structure and body scale. Sequence analyses of both ITS and LSU regions reveal the significant genetic divergence between H. bohaiensis and other established species in this genus, further supporting novelty of this species. Noticeably, different sample treatment methods resulted in morphological variation of the apical pore complex(APC) of H. bohaiensis, which needs to be taken into account in future study.     

强光照和黑暗条件下刺参体壁的基因表达差异

参对光照变化非常敏感,研究刺参对光照的分子响应非常重要。本研究应用RNA测序获取了刺参暴露于强光（“强光”）、正常光照（“对照”）和完全黑暗（“黑暗”）环境下体壁的基因表达谱情况,通过“对照”与“黑暗”,“对照”与“强光”和“黑暗”与“强光”的比较,在|log₂ ratio|≥1和FDR≤0.001的标准下分别发现了1161、113和1705个差异表达基因（DEGs）。基因本体分析表明,“cellular process”和“binding”在“生物过程”和“分子功能”类别中的DEGs富集最多,而“cell”和“cell part”在“细胞组分”类别中的DEGs富集最多。将DEGs与Kyoto Encyclopedia基因和基因组数据库上的于214、41和229条通路进行比对,发现了51、2和57条通路分别显著富集。本研究发现的光特异性DEGs可作为深入研究刺参对光照变化的生化适应机制的重要目标基因。     

An experimental study on water entry of asymmetric wedges

The aim of the paper is to provide an experimental reference for investigation of asymmetric water entry of wedges. Parameters of the study include initial deadrise angle, inclination angle and impact speed. Initial deadrise angles of the wedges were 20° and 30° with inclination angles ranging from 0° to 15° in 5° increments. Wedges were freely fallen from three different heights. Time histories of impact pressure and body acceleration were recorded. Sampling rate of measurements were set to 25 KHz. Main configuration of each test including mass of the wedge and water level were kept unchanged during all experiments. Additionally, several calibration tests were conducted to assess the repeatability and accuracy of the recorded data. The experimental results are compared with different entry theories and other available experiments. The comparison shows a reasonable agreement and indicates that the inclination angle can dramatically affect the impact pressure experienced by the wedges. Finally, the results show that the traditional asymmetric theories are not appropriate for all inclination angles.     

CFD investigations of OXYFLUX device,an innovative wave pump technology for artificial downwelling of surface water

No other environmental variable of such ecological importance to estuarine and coastal marine ecosystems around the world has changed so drastically, in such a short period of time, as dissolved oxygen in coastal waters. The prevalent methods for counteracting anoxic sea events are indirect measures which aim to cut-down anthropic loads introduced in river and marine environments. To date, no direct approaches, like artificial devices have been investigated except the WEBAP and OXYFLUX devices. The present paper adopts a numerical approach to the analysis of the pumped surface water as well as the analysis of the dynamic response of the OXYFLUX device. By means of a CFD-RANS code and through the application of overset grid method, the 1/16 OXYFLUX model’s dynamic response and pumping performance are evaluated. The appropriate grid is selected after an extensive sensitivity analysis carried out on 9 different grids. The CFD model is validated by comparing numerical and physical results of heave decay test, heave response, and surface water discharge under the action of regular waves. The extensive comparison with experimental results shows consistently accurate predictions. The main findings of the study show that nonlinear effects remarkable reduce the dynamic behaviour of the OXYFLUX and generate an unexpected second harmonic for pitch response intensifying the overtopping discharge also for small waves caused by the summer's low intensity winds.     

改进型中心管模型能量转换性能试验及样机设计

为了提高中心管振荡水柱波浪能利用技术能量转换效率,基于新的认识和目前常用的2.4米导航灯标,对中心管尾部设计了三种模型并在造波水槽中进行能量转换性能试验。试验结果表明:直管型中心管俘获宽度比最高达到了70.25%,但通频带宽度窄;加长喇叭口型中心管略好于喇叭口型中心管;在喷咀比为0.02条件下,加长喇叭口型中心管浮体有较高双峰俘获宽度比,波峰为40.0%,波谷为31.6%,通频带宽,为随机波下高效转换创造了条件。最高俘获宽度比和双峰通频带特性实验数据结果都优于历史文献值。根据试验数据对一些适合小型海洋仪器供电的样机进行了设计,设计的样机具有较高的性价比。     

天津海域围填海工程对渤海湾水交换的影响研究

利用三维海洋数值模型FVCOM,进行渤海湾三维水动力和水交换数值模拟,经实测潮汐和潮流资料验证,模型模拟结果较好。然后采用该模型对渤海湾内的水体水交换能力进行定量研究。研究结果表明,在天津海域进行围填海工程之前渤海湾水体的半交换周期为300 d左右,围填海之后,水体半交换周期延长25 d,渤海湾西部水体的水交换率下降可达10%,半交换周期延长92 d。尤其是天津沿海南部海域的水交换能力下降严重,围填海之后其水体半交换周期延长可达200 d。渤海湾北部也有部分海域水交换周期延长达200 d。建议在进行围填海工程建设时,应将工程对水体交换能力的影响纳入考虑,避免因围填海工程因素造成的恶劣环境影响。     

水下土体剪切界面摩擦力试验研究

基于在自行改进设计的水下土体剪切界面摩擦力测定装置进行的室内试验结果,对不同粒组、含水率及剪切速率条件下土体剪切界面抗剪强度和摩擦力进行了研究。试验结果表明,不同土体间剪切界面抗剪强度和摩擦力与土体颗粒粒组、含水率相关性表现不同。随着含水率的增加,粉砂抗剪强度和摩擦力呈增加趋势,细砂、中砂抗剪强度和摩擦力受含水率变化影响较小。土体颗粒粒组不同,抗剪强度和摩擦力有明显差异,表现在粉砂大于细砂和中砂,小于黏粒含量较高的粉土,细砂和中砂的差别较小。试验条件下得到的粉砂、细砂、中砂土体与粉质土底床间的水下剪切界面摩擦力值在0.2~1.0 kPa区间,并结合海洋地质实例说明界面摩擦力的应用。     

西太平洋雅浦海沟区海底热流原位测量

为了解西太平洋雅浦海沟区俯冲带及岛弧两侧的海底热流分布情况,2015年1-3月期间的西太平洋海山航次中,"科学"号利用最新一代Lister型热流探针对该区域开展了海底热流原位测量工作。沿着横跨俯冲带的站位测线,本次进行了10个热流站位测量,共获得8个站位的有效数据。测量结果表明,该地区地温梯度的变化范围为0.011~0.137 Km-1,平均0.089 Km-1,热导率的变化范围为0.58~1.32 Wm-1K-1,热流值则在14.4~118.85 mWm-2之间,平均为68.02 mWm-2。热流值分布显示,雅浦岛弧附近具有较高的热流值,自雅浦岛弧向两侧热流值逐渐降低,低热流区主要分布在帕里西维拉海盆。由于该地区热流测量数据依然稀缺,深入的讨论有待进一步研究。     

脱脂南极磷虾粉对圆斑星鲽幼鱼体组成和消化性能的影响

为探讨脱脂南极磷虾(Euphausia superba)粉对圆斑星鲽(Verasper variegatus)幼鱼体组成和消化性能的影响。作者以鱼粉和脱脂磷虾粉为蛋白源、鱼油为脂肪源,用脱脂磷虾粉蛋白替代0%、10%、20%、30%、40%、50%和100%的鱼粉蛋白,设计7种等蛋白(50%)、等脂肪(8%)的实验饲料(分别表示为F0、F10、F20、F30、F40、F50和F100)。每组设3个重复,每个重复20尾鱼,初始体质量为(68.13±0.20)g,经过8周的饲养实验。结果表明:F100组的肝体比和脏体比显著低于其他各组(P0.05),F20~F30组的肥满度显著高于F40组(P0.05);F100组全鱼的水分和灰分显著高于其他各组(P0.05),F10组和F100组全鱼的粗蛋白显著高于其他各组(P0.05),F30组和F50组全鱼的粗脂肪显著高于其他各组(P0.05),F100组全鱼的粗脂肪显著低于其他各组(P0.05)。F10、F20和F50组肌肉的水分显著高于F100组(P0.05),F20组和F30组肌肉的粗脂肪显著高于其他各组(P0.05),各替代组肌肉的粗蛋白、灰分无组间差异(P0.05);胃蛋白酶活性组间无差异(P0.05),F0和F20的胃脂肪酶活性显著低于F30组(P0.05),F30组的胃淀粉酶活性显著高于其他各组(P0.05),F30和F100组的肠脂肪酶活性显著低于其他各组(P0.05),F0组的肠淀粉酶活性显著高于其他各组(P0.05);脱脂南极磷虾粉替代组的皱襞高度呈现先升高后降低的趋势,F30组的皱襞高度显著高于其他组(P0.05),替代组和对照组的黏膜厚度和绒毛长度没有显著性差异(P0.05)。综合来看,脱脂南极磷虾粉10%~50%的替代比例对圆斑星鲽幼鱼的形态指标、体组成、消化酶活性和肠道形态指标有较好的效果。