Tracing contamination sources through phenotypic characterization of Escherichia coli isolates from surface water and groundwater in an agro-ecosystem

The objective of this research was to evaluate the groundwater chemical and bacteriological quality in the Barranquita-Knutzen basin (Córdoba, Argentina). The main purpose was to trace contamination through the examination of bacteriological parameters and antibiotic resistance of Escherichia coli, relating them to surface water, hydrogeological features and land use. Thus, 40 water samples were collected. The major chemical components and bacterial indicators were determined and antibiotic resistance was analysed using standard methods. Multivariate factorial analysis showed that the first principal component (PC) reveals the process of water mineralization, while the second and the third PCs explain a low percentage of variance, but collect chemical constituents and total and faecal coliform bacteria, respectively, revealing specific contamination situations. The observed antibiotic resistance profiles of E. coli and their relation with the land uses revealed that the source of faecal contamination in water is mainly from animal residues.     

再生混凝土的低温抗震性变化对比分析

再生混凝土作为建筑材料在被使用前,需要对其低温抗震性能做出分析。但目前我国是全球新建筑数量最多的国家,再生混凝土成本低、需求量大,建筑方根据对普通混凝土的印象,直接将再生混凝土应用到建筑工程建设中,其中存在低温抗震性能不明确的问题。针对这个问题,提出再生混凝土的低温抗震性变化对比分析,通过再生混凝土的材料配比及实验搭建设计再生混凝土低温抗震结构模型。根据其指标对其低温抗冻性能、加速度变化、损伤指标、刚度稳定性、层位移变化等几个方面进行变化对比分析。对比实验结果表明:再生混凝土当含气量在逐渐降低时,其保温效果降低,强度在降低,抗冻性也在降低,耐久性和稳定性较差,并且在地震后楼层间的位移和加速度变化较大,不能保证楼层的稳定性;位移角无法降低到最小;随着位移和负载地震力不断增加,再生混凝土逐渐产生开裂变化,刚度减小速度较快,刚度退化程度较大,低温抗震性能较差。     

建筑结构的屈曲约束支撑对其抗震加固性能的影响

当前方法采用伸臂桁架加固建筑结构时,未考虑建筑结构的屈曲约束支撑力的影响,伸臂桁架与建筑结构的连接不牢固,导致其对建筑结构的抗震加固性能较差。故此,深入分析建筑结构的屈曲约束支撑对其抗震加固性能的影响,设计建筑结构抗震加固方案,利用高强螺栓节点经由连接钢板实现屈曲约束支撑与建筑结构的铰接固定。分别从支撑变形同建筑结构层间位移的关系、建筑结构支撑承载力、多遇地震影响下屈曲约束支撑框架的位移验算,以及罕遇地震影响下屈曲约束支撑的弹塑性位移验算方面,分析屈曲约束支撑对建筑结构抗震加固性能影响。经实验分析得出,建筑结构加入屈曲约束支撑后第一扭转周期同第一平动周期的比值降低0.14,X、Y两个方向的砌体墙同建筑结构的刚度比值降低6.9、8.0,最大顶点位移值降低15.4 mm、29.3 mm,抗震加固性能大大提高。     

Species selection and assessment of eco-engineering effects of seedlings for biogeomorphological landscape experiments

Landscape experiments of fluvial environments such as rivers and deltas are often conducted with live seedlings to investigate effects of biogeomorphological interactions on morphology and stratigraphy. However, such experiments have been limited to a single species, usually alfalfa (Medicago sativa), whereas important environments in nature have many different vegetation types and eco-engineering effects. Landscape experimentation would therefore benefit from a larger choice of tested plant species. For the purpose of experimental design our objective was to identify fast-germinating and fast-growing species and determine their sensitivity to flow conditions during and after settling, their maximum growth, hydraulic resistance and added bank strength. We tested germination time and seedling growth rate of 18 candidate species with readily available seeds that are fast growing and occur at waterlines, plus Medicago sativa as a control. We selected five species that germinate and develop within days and measured properties and eco-engineering effects depending on plant age and density, targeting typical experimental conditions of 0–0.3 m/s flow velocity and 0–30 mm water depth. Tested eco-engineering effects include bank strength and flow resistance. We found that Rumex hydrolapathum can represent riparian trees. The much smaller Veronica beccabunga and Lotus pedunculatus can represent grass and saltmarsh species as they grow in dense patches with high flow resistance but are readily erodible. Sorghum bicolor grows into tall, straight shoots, which add significantly to bank strength, but adds little flow resistance and may represent sparse hardwood trees. Medicago sativa also grows densely under water, suggesting a use for mangroves and perhaps peat. In stronger and deeper flows the application of all species changes accordingly. These species can now be used in a range of landscape experiments to investigate combined effects on living landscape patterns and possible facilitation between species. The testing and treatment methodology can be applied to new species and other laboratory conditions. © 2019 The Authors. Earth Surface Processes and Landforms Published by John Wiley & Sons Ltd. © 2019 The Authors Earth Surface Processes and Landforms Published by John Wiley & Sons Ltd © 2019 The Authors Earth Surface Processes and Landforms Published by John Wiley & Sons Ltd.     

装配式剪力墙结构抗震试验研究

近年来我国各区域地震频发,房屋在地震中受损较为严重,如何提升房屋的抗震性是目前亟待解决的问题。以某市的装配式剪力墙高层住宅结构试点工程为试验对象,对其剪力墙结构进行设计改进,通过反复多次的荷载试验,分析其破坏受损过程,验证改进结构的抗震性能。试验结果表明:改进型的装配式剪力墙结构具有较好的抗震性能,应用推广前景良好。     

基于软化拉-压杆模型的RC空间边节点抗剪承载力计算方法

实际地震作用是多维的、随机的,框架节点在2个方向同时受力,其抗震能力相比于单向地震作用会降低。目前,对节点核心区在2个方向同时受力时抗剪承载力的研究尚未完善。在双向受力下,节点核心区2个方向传来的剪力形成合剪力作用面,在节点核心区内部形成不同于单向受力下的斜向斜压杆。本文基于软化拉-压杆模型,针对水平双向受力相同的空间边节点,在合剪力作用面内建立抗剪承载力计算模型,确定空间边节点核心区斜压杆以及钢筋拉杆的计算方法。利用有限元软件建立不同参数空间边节点模型,并收集部分空间节点试验数据,将剪力计算值与模拟值或试验值进行对比。结果表明,基于软化拉-压杆模型建立的双向受力下钢筋混凝土框架,其边节点抗剪承载力计算值与模拟值或试验值吻合良好。     

PT型袖珍贯入仪在海岸带地区工程地质调查中的应用

通过对莱州湾地区工程地质勘察中大量袖珍贯入试验与静力触探试验、标准贯入试验、土工试验数据的对比分析,采用数理统计方法,建立了PT型袖珍贯入阻力与静力触探试验指标、标准贯入试验击数、土的液性指数及压缩模量相关公式。为今后在海岸带地区工程地质勘察中,采用PT型袖珍贯入仪对岩心的现场贯入试验,及时准确地判定土的物理力学性质提供了依据。     

Intersection-pavement de-icing: comprehensive review and the state of the practice

Winter maintenance operations are crucial for pedestrian and motorist safety and public mobility on urban streets and highways in cold regions, especially during winter storms. This study provides a comprehensive literature review of existing deicing technologies, with emphasis on electrical resistance-heating deicing technologies for possible applications in areas with concentrated traffic, such as street intersections and crosswalks. A thorough review of existing and emerging deicing technology for snow/ice melting was conducted. The performance of various deicing methods was evaluated and the installation and operation cost of the electrical resistance-heating methods compared. Finally, current state of the practice of intersection/crosswalk winter maintenance was surveyed among state departments of transportation in North America. The intersection/crosswalk winter maintenance procedure adopted by the State of Alaska Department of Transportation and Public Facilities was described, and the annual winter maintenance and operation cost per intersection was estimated. It was found that the annual energy cost of an electrical resistance-heating method such as the carbon-fiber-tape deicing technology is about the same as the average annual maintenance and operation cost of current practice. In addition, an automatic electrical resistance-heating deicing system will bring benefits such as minimized delay time and improved safety for pedestrian and vehicular traffic in an urban application.     

一株副溶血弧菌D3112致病性和抗药性的表征

副溶血弧菌广泛分布于海洋环境中,虽然大多数环境菌株并非致病菌,但它们常常携有毒力基因,从而具有潜在的致病性。本文从黄海(山东青岛)海水中分离到一株副溶血弧菌D3112,对其进行了全基因组测序、溶血实验和人工感染实验等。基因组测序分析发现D3112并不含有副溶血弧菌的致病性标志溶血素基因tdh和trh,这与PCR检测结果一致,但含有其他溶血素基因以及多种毒力相关基因。生物学实验显示,该菌可产生明显的溶血现象,而且具有蛋白酶、明胶酶、脂酶和淀粉酶活性,但是缺少卵磷脂酶活性。人工感染实验表明副溶血弧菌D3112具有致病性,感染斑马鱼的半致死剂量约为5×105CFU。药物敏感实验证明了D3112具有多重耐药性。本文对海洋环境中的副溶血弧菌D3112同时开展了基因型和表型研究,为准确评价环境细菌的潜在致病性提供了有用的数据信息。