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351.
Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology, quantitative genetics and genomics. Therefore, it is extremely necessary to select several versatile, low-cost, efficient and time- and labor-saving methods to develop a large panel of microsatellite markers. In this study, we used Zhikong scallop (Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency, while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time- and cost-saving, it is difficult to obtain a large number of microsatellite markers, mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study, we recommend two methods, microsatellite-enriched library construction method and FIASCO-colony hybridization method, for large-scale microsatellite marker development. Both methods were derived from the mi-crosatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes. 相似文献
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353.
海洋资源承载力指标体系的设计与评价 总被引:2,自引:0,他引:2
刘蕊 《广东海洋大学学报》2009,29(5):6-9
在探讨海洋资源承载力指标体系设计原则的基础上,构建了科学、系统、可操作的海洋资源评价指标体系。运用专家咨询法筛选指标,对广东省的海洋资源承载力进行分析,指出广东省海洋资源的承载力在逐年下降,广东各地的海洋资源承载力各不相同,应依据海洋资源承载力对海洋经济政策进行选择。 相似文献
354.
东海捕虾桁拖网选择性能不佳,对渔业资源的保护和渔业的合理管理带来了负面的影响.刚性栅栏是实现拖网渔具种类选择性捕捞的主要装置,本文根据在吕四渔场开展刚性栅栏海上生产试验,结合SELECT模型分析不同栅条间距(15、20和25mm)的刚性栅栏对短吻舌鳎、小黄鱼和棘头梅童鱼的分隔效率.结果表明,随着栅条间距的增大,栅栏对鱼类的重量分隔率逐渐减小,分隔栅栏对鱼类的50%选择体长(L50S))逐渐增大;对于短吻舌鳎,个体接触分隔栅栏的概率可使用常数来表示,即接触概率与个体尺寸无关,接触概率在0.2-0.4之间,并随着栅条间距的增大而增大(不显著,P>0.05):对于小黄鱼和棘头梅童鱼,所有进入网囊的个体都将接触刚性栅栏;不同鱼类接触栅栏概率的显著差异说明不同鱼类在网囊中的不同行为习性. 相似文献
355.
356.
Natural assemblages of marine bacteria were chosen in a batch culture experiments. The impact of varying nitrogen substrate concentrations and the substrate C:N ratios (C:NS) on the bacterial C:N ratio (C:NB), the bacterial growth efficiency (BGE) and ammonium regeneration was mainly examined. The C:NS ratios varied from 5:1 (carbon limitation) to 40:1 (nitrogen limitation) with varying combinations of glucose and NO3-. The C:NB ratio had positive relationship with the C:NS ratio (r=0.93, n=8), whose value was 3.77 when the C:NS ratio was 5:1 but increased to 6.47 when the C:NS ratio was 40:1. These results indicate that the C:NB ratio is a potential diagnostic tool for determining the bacterial growth in natural waters controlled by either, carbon or nitrogen. BGE decreased with the declining nitrate concentration and negatively related to C:Ns (r=-0.51, n=8). The average value of BGE was 0.20. This value was a little lower than other reports, which could be induced by the nitrogen source used in our experiments. Finally, regeneration time of ammonium delayed with the increasing C:NS ratio, which indicates that there were different metabolism mechanisms when bacterial growth was limited by carbon source and nitrogen source. 相似文献
357.
358.
为了全面评价和考核湘江长沙段各洲滩近几年来采取的不同灭螺措施对洲滩钉螺(Oncomelania hu-pensis)螺情的影响,采用以系统抽样结合环境抽查的查螺方法,2003~2008年对湘江长沙段20个有螺洲滩进行了跟踪调查.结果表明,2008年与2003年相比,活螺洲滩数、有螺框出现率、钉螺平均密度和活螺率分别下降了40.00%,99.10%,99.74%,51.09%.经统计学处理,各洲滩有螺框出现率(Z=91.560 2,P<0.01)、活螺率(Z=65.133 2,P<0.01)不相同,但年度下降趋势明显.在20个有螺洲滩中,有感染性钉螺分布的洲滩5个,占有螺洲滩总数的25.00%;有感染性钉螺分布的洲滩逐年减少,感染性钉螺的平均密度和感染率逐年降低,2007~2008年所有洲滩无感染性钉螺分布.湘江长沙段有螺洲滩采取的各种防治措施中,药物与环改灭螺相结合的效果远好于单纯的药物灭螺.各种环改灭螺措施,又以抬洲降滩、翻耕垦种的灭螺效果更持久,更彻底. 相似文献
359.
Fuzzy comprehensive evaluation model for water resources carrying capacity in Tarim River Basin, Xinjiang, China 总被引:12,自引:0,他引:12
This paper explores the method of comprehensive evaluation of water resources carrying capacity and sets up an evaluation model applying the fuzzy comprehensive evaluation method. Based on the data of nature, society, economics and water resources of the Tarim River Basin in 2002, we evaluated the water resources carrying capacity of the basin by means of the model. The results show that the comprehensive grades are 0.438 and 0.454 for Aksu and Kashi prefectures respectively, where the current water resources exploitation and utilization has reached a relative high degree and there is only a very limited water carrying capacity, 0.620 for Kizilsu Kirgiz Autonomous Prefecture, where water resources carrying capacity is much higher, and in between for Hotan Prefecture and Bayingolin Mongolian Autonomous Prefecture. As a whole, the comprehensive grade of the Tarim River Basin is 0.508 and the current water resources exploitation and utilization has reached a relative high degree. Thus, we suggest that the integrated management of the water resources in the basin should be strengthened in order to utilize water resources scientifically and sustainably. 相似文献
360.
On the basis of the soil environment investigation in Da'an City, Jilin Province, China, 40 soil samples from main land use types were obtained and tested by standard method. Soil organic matter (SOM), total N (TN), total P (TP), total K (TK), available N (AN), available P (AP) and available K (AK) were chosen as the evaluation factors. A regional soil nutrient evaluation model was developed based on the matter-element model. The results show that the soil samples with nutrient grade Ⅱ-Ⅴ respectively account for 10%, 30%, 32.5% and 27.5%, and those with grade Ⅳ and Ⅴ account for 60% in all samples. The relationship between soil nutrients and land types indicates that the nutrients of farmland are relatively good, with 41.7% of soil samples with the nutrient grade Ⅳ and Ⅴ. The nutrients of saline-alkali land and sandy land are the worst, with 100% of soil samples with the nutrient grade Ⅳ and Ⅴ. And the ratios of soil samples grade Ⅳ and Ⅴ in grassland and wasteland are respectively 62.5 % and 54.55%. Generally speaking, the soil nutrients status in Da'an City is poor, 60% of soil samples are in poor and extremely poor conditions, indicating that the soil has been severely eroded. Being a relatively superior evaluation method with more accurate resuits and spatial distribution consistency, matter-element analysis is more suitable for regional soil nutrient evaluation than previous models. 相似文献