乌苏里拟鲿野生群体和人工养殖群体遗传多样性的比较研究

采用SRAP分子标记技术对乌苏里拟鲿(Pseudobagrus ussuriensis)1个野生群体(采自江苏省洪泽湖)和2个人工养殖群体(分别采自淮安市水产研究所F2代和苏州市东山水产养殖场F3代)进行了遗传多样性的比较.结果显示:从100组引物组合中筛选出12组条带清晰、多态性好的引物组合,每个引物组合检测到11～...     

羊鲍野生群体遗传多样性与分化的研究

运用随机扩增片段长度多态性DNA标记(RAPD)技术对中国海南省英州、安游和亚龙湾海域的3个羊鲍(Haliotis ovina)野生群体进行遗传多样性与分化的研究.利用14个10bp随机引物,在90个个体中共检测到72个位点,其中英州,安游和亚龙湾群体的多态位点数分别为65,61和62个,多态位点百分数(P)分别为90...     

Impact of oxygen-depleted water on the vertical distribution of chaetognaths in the northeastern Arabian Sea

The influence of a thick layer of oxygen-depleted water (<0.2 ml l⁻¹) on the abundance and distribution of chaetognaths was investigated in the northeastern Arabian Sea (NEAS), a natural oxygen-deficient system in the global ocean. The species and maturity stage-wise distribution of this group were studied at five discrete depths down to 1000 m. A total of 22 species belonging to four genera were observed, and the genus Sagitta dominated, representing 60% (500-1000 m) to 89% (Mixed layer depth) of the total chaetognath population. Based on their vertical distribution limits, four groups were recognised, as follows: I: species abundant in surface water with a maximum distribution limit up to 300 m; II: species confined mainly to deeper waters (>500 m); III: species present throughout the water column (0-1000 m); and IV: species present in most layers, but with a preference for a specific depth stratum. A positive correlation (P<0.01) was observed in the abundance of chaetognaths and their main prey copepods, emphasising the strong trophic relationship between these groups. It was found that the intensely oxygen-deficient waters of the NEAS play a crucial role in the vertical distribution and abundance of chaetognath species of all four genera. This report presents information on the maturity stages and ontogenetic migration of this important planktonic group in relation to the oxygen-depleted water in the study region for the first time. The results obtained are also important for understanding the biological processes associated with a major oxygen minimum zone (OMZ) in the global ocean.     

东南太平洋热液区硫化物样品富集培养物中Fe3＋还原细菌的多样性分析

采集了东南太平洋热液区DYl15-20V一$35一TVGl7站位的沉积物硫化物样品,其含有76．717％（m／m）强还原性的磁性良好的黄铁矿．以乳酸钠为电子供体,不溶性的氢氧化铁为电子受体,对该样品进行Fe¨还原细菌的定向富集培养．通过构建该茵群的16SrDNA基因文库,随机挑选82个阳性克隆子进行群落结构多样性分析．研究结果表明：82个克隆代表的6种基因型分别属于4个主要类群,其中ε一变形杆菌占有绝对优势,达到55．2％．此外其还包括厚壁菌门、β一变形杆菌和仅．变形杆菌,克隆子比例依次为27．6％、10．3％、6．9％．在富集产物中还原氢氧化铁的功能菌群主要是具有硫还原作用的ε一变形菌中的硫还原菌．     

Comparison of two sampling methods when studying periphyton colonization in Lam Tsuen River, Hong Kong, China

We used standard periphyton samplers to examine the colonization pattern of periphytic algae on artificial substrates (glass slides) in Lam Tsuen River, Hong Kong, in dry (winter) and wet (summer) seasons. In each season, six replicated slides were retrieved randomly and replaced by new slides at weekly intervals over a period of 6 weeks. We thus obtained two batches of slides, both with a series of different exposure times (1 to 6 weeks): one batch was set up at the same time (start of the sampling) and the other was harvested at the same time (end of the sampling). Changes in taxonomic composition, species diversity (Shannon-Wiener diversity index), standing crop (in terms of cell density and cell biovolume), and abundance of the abundant algal species were monitored and compared between the two batches of slides. The succession patterns of the periphytic algae were similar between the two batches in each season, while more remarkable differences were observed between the two seasons, suggesting that either batch would be suitable for a colonization study of periphytic algae. The cell density was dominated by diatoms in both seasons, while the cell biovolume was dominated by diatoms in winter and by green algae in summer. The cell density and biovolume attributed to blue green algae was relatively small. Most of the diatom species exhibited similar colonization patterns throughout the experiment, while green algae showed different succession patterns in different seasons or sampling methods, indicating that diatoms are better bio-indicators than green algae for a periphyton colonization study. In general, the diversity indices and the standing crops reached their maximums at around week 4, and they were higher in summer than in winter.     

塔里木河下游土壤水分和地下水位变化对植被的影响

Based on data collected over five years of monitoring the Lower Tarim River,we analyzed the variability of soil moisture content (SMC) and the relationship between SMC,groundwater table depth (GWD) and vegetation by using the methods of coefficient of variation (Cv),Pearson correlation and regression. The results of the variability of SMC indicate that it rose with increase in depth of soil layer -SMC in the soil layer of 0-60 cm was relatively small compared to SMC in the soil layer of 100-260 cm which showed a significant increase in variability. SMC and GWD before and after ecological water diversions exhibited significant differences at the site of the Yingsu transect and its vicinity of the watercourse,especially SMC in the soil layer of 100-260 cm increased significantly with a significant rise of GWD and reached maximum values at a GWD of about 4 m. Plant coverage and species diversity significantly improved with increases in SMC in the soil layer of 100-260 cm,both of them approached the maximum values and 92.3% of major plant species were able to grow when SMC was > 10%. To restore the ecosystem of desert riparian forest along the Lower Tarim River,the GWD must be maintained at < 4 m in the vicinity of the watercourse and at about 4 m for the rest of this arid region.     

福建省维管束植物物种多样性及其分布格局

截至2011年7月,福建省发现的维管束植物有250科1611属4839种（含亚、变种）,是中国植物多样性较为丰富的省份之一．福建维管束植物主要集中分布在武夷山脉和戴云山脉地区,其中武夷山脉北段具有物种丰富度高、特有种多等特点,是中国11个具有全球意义的生物多样性保护的关键区之一．武夷山脉中段是福建省自然保护区分布最为密集的区域,也是福建省植物物种多样性最为丰富的地区．以福建戴云山国家级自然保护区为核心的戴云山脉中段,分布着大面积的南亚热带季风常绿阔叶林,有着丰富的植物多样性,位于戴云山脉南段的博平岭则是福建省南亚热带雨林森林生态系统的典型分布区,其植物种类在福建省植物物种多样性中独树一帜．分布在福建滨海湿地的维管束植物虽然种类不多,但地域特色显著,是福建维管束植物的重要组成部分．     

The variation in soil moisture and the appropriate groundwater table for desert riparian forest along the Lower Tarim River

Based on data collected over five years of monitoring the Lower Tarim River, we analyzed the variability of soil moisture content (SMC) and the relationship between SMC, groundwater table depth (GWD) and vegetation by using the methods of coefficient of variation (Cv), Pearson correlation and regression. The results of the variability of SMC indicate that it rose with increase in depth of soil layer – SMC in the soil layer of 0–60 cm was relatively small compared to SMC in the soil layer of 100–260 cm which showed a significant increase in variability. SMC and GWD before and after ecological water diversions exhibited significant differences at the site of the Yingsu transect and its vicinity of the watercourse, especially SMC in the soil layer of 100–260 cm increased significantly with a significant rise of GWD and reached maximum values at a GWD of about 4 m. Plant coverage and species diversity significantly improved with increases in SMC in the soil layer of 100–260 cm, both of them approached the maximum values and 92.3% of major plant species were able to grow when SMC was > 10%. To restore the ecosystem of desert riparian forest along the Lower Tarim River, the GWD must be maintained at < 4 m in the vicinity of the watercourse and at about 4 m for the rest of this arid region.     

Spatial and temporal variability of soil properties under Caragana microphylla shrubs in the northwestern Shanxi Loess Plateau, China

Shrub-induced spatial and temporal heterogeneity of soil properties is common in arid and semiarid ecosystems, and it facilitates the development of plant species diversity. We selected 5-, 10-, 20-, 30-, and 40-year-old Caragana microphylla shrubs in the Shanxi Loess Plateau to evaluate the spatial and temporal heterogeneity of soil properties under and outside the shrub canopy. In addition, the presence of adventitious plant species was investigated to assess the development of herbaceous species diversity. Soil samples were collected from two depths (0-5 cm and 5-10 cm). The establishment and development of shrubs promoted temporal variation, improved soil texture, enhanced soil organic matter (SOM), total nitrogen (TN), and cation exchange capacity (CEC), and decreased pH, bulk density (BD), and soil water content (SWC). The results further confirmed that SOM, TN, and CEC were significantly higher at the center than at the outside of the shrub canopies (P < 0.05) and were higher at the 0-5 cm depth than at the 5-10 cm depth. Moreover, the differences in SOM, TN, and CEC from the center to the outside of shrub canopies were greater under 30- and 40-year-old shrubs than under 10- and 5-year-old shrubs. Furthermore, the spatiotemporal heterogeneity of the soil properties facilitated the development of herbaceous species diversity.     

Microbial Diversity of the Hypersaline Sidi Ameur and Himalatt Salt Lakes of the Algerian Sahara

Microbial populations within hypersaline lakes often exhibit high activities of photosynthesis, dissimilatory sulphate reduction and other processes and, thus, can have profound impacts on biogeochemical cycles of carbon, nitrogen, sulphur and other important elements within arid lands. To further understand these types of ecosystems, the physicochemical and biological properties of Sidi Ameur and Himalatt Salt Lakes in the Algerian Sahara were examined and compared. Both lakes were relatively neutral in pH (7.2-7.4) and high in salt, at 12% and 20% (w/v) salinity for Himalatt and Sidi Ameur Lakes, respectively, with dominant ions of sodium and chloride. The community compositions of microbes from all three domains (Bacteria, Archaea and Eukarya) were surveyed through the use of 16S and 18S ribosomal gene amplification and clone library clustering using amplified ribosomal DNA restriction analysis (ARDRA) in conjunction with DNA sequencing and analysis. A high level of microbial diversity, particularly among the bacteria of the Himalatt Salt Lake and archaea of Sidi Ameur Lake, was found within these environments. Representatives from all known halophilic bacterial phyla as well as 6 different genera of halophilic archaea were identified. Moreover, several apparently novel phylotypes among both archaea and bacteria were revealed.