南极菲尔德斯半岛土壤微生物在富集传代培养中对菲的响应

为揭示富集传代培养过程中南极多环芳烃降解菌群的演替规律,以菲为唯一碳源和能源对南极菲尔德斯半岛不同地理位置的6份土壤样品进行了富集和连续传代培养,并采用高通量测序技术分析了富集传代培养过程中菲降解菌群的群落结构及生物多样性。Alpha多样性分析结果表明,在富集培养阶段南极菲降解菌的物种丰富度显著高于传代培养阶段(P0.05),但传代培养阶段的代际物种丰富度无显著差异(P0.05)。Beta多样性分析结果表明富集培养阶段与传代培养阶段的群落结构具有显著性差异(P0.05),但传代培养阶段菲降解菌群趋于相对稳定,代际间群落结构无显著性差异(P0.05)。大量微生物在富集培养阶段丰度较高,但因不能适应实验室培养条件或不能利用菲而在传代培养阶段被淘汰。反之,假单胞菌属(Pseudomonas)、鞘氨醇菌属(Sphingobium)、贪噬菌属(Variovorax)、甲基娇养杆菌属(Methylotenera)和产碱杆菌属(Alcaligenes)等适应了该选择压力并在传代培养阶段形成了动态平衡的优势种群。此外,Pseudomonas、Sphingobium和Variovorax等在富集传代培养过程中具有显著性差异的优势种在维持群落的动态平衡中也发挥着重要的作用。该研究有助于加深人们对南极土壤环境中持久性有机污染物的降解潜力以及关键微生物动态变化的认识,为下一步充分挖掘利用南极环保功能微生物资源提供重要的依据。     

黄河口近岸海域鱼卵、仔稚鱼种类组成及群落结构特征

为了解调水调沙期间黄河口及邻近海域鱼卵、仔稚鱼群落结构的动态变化,作者分别于2011年和2018年黄河调水调沙期间（6月—7月）,利用大型浮游生物网对该海域的鱼卵、仔稚鱼进行表层水平拖网调查。结果表明：2011年3个航次共采集鱼卵1 280粒,仔稚鱼5 973尾,隶属于7目11科13属;2018年3个航次共采集鱼卵4 104粒,仔稚鱼237尾,隶属于7目11科13属。优势种以斑鰶（Konosirus punctatus）、鳀（Engraulis japonicus）和赤鼻棱鳀（Thryssa kammalensis）等短生命周期、低营养层级的小型中上层鱼类为主。靠近黄河入海口处站位的鱼卵、仔稚鱼密度相对较高,鱼卵、仔稚鱼分布范围向黄河入海口门处集中。鱼卵、仔稚鱼的多样性指数在调水调沙过程中出现波动,过后又恢复至调水调沙前水平。环境因子Pearson相关性分析表明,鱼卵数量与环境因子间无显著相关性（P0.05）,仔稚鱼数量与叶绿素a的含量呈极显著正相关（R=0.870,P<0.01）。两个年度调查结果对比发现,总体来说调查期间黄河口及邻近海域鱼卵、仔稚鱼种类数变化不大,在适温类型组成上均以暖温种为主;2018年鱼卵、仔稚鱼采集数量均小于2011年,但是鱼卵、仔稚鱼的均匀度指数（J’）和多样性指数（H’）均大于2011年,除6月上旬航次外,其他两个航次丰富度指数（D）也均大于2011年。本研究补充了黄河口及邻近海域鱼类早期补充资源的基础数据,旨为黄河口邻近海域及渤海的渔业资源评估和养护提供依据。     

倒立水母Cassiopea andromeda 的POU、Hox基因家族的多样性及系统发育分析

与其他钵水母相比,倒立水母具有独特行为：在生命周期的大部分时间里,它都在海底呈倒立附着、“睡眠”的状态。为探索与这一独特行为相关的遗传信息,本研究对安朵仙水母(Cassiopea andromeda)和同属于钵水母纲的海蜇(Rhopilema esculentum)、巴布亚硝水母(Mastigias papua)进行首次全基因组测序、拼接和注释,并重点分析了这3种钵水母与感觉功能、神经系统发育相关的重要转录调节因子Hox和POU基因家族的多样性与系统发育关系。遗传分析显示,刺胞动物门中Hox和POU基因家族具有明显的物种间差异性。对Hox基因的分析首次发现钵水母及水螅(Hydra vulgaris)的“前段Hox基因”产生了部分缺失,并进一步印证了刺胞动物不存在“中段Hox基因”的假说。安朵仙水母和海蜇具有相对全面的ParaHox基因种类,即GSX和XLOX/CDX基因,而巴布亚硝水母只有GSX基因。在POU基因多样性方面,安朵仙水母、海蜇、星状海葵(Nematostella vectensis)的基因组具有全部4类POU,而巴布亚硝水母、海月水母(Aurelia aurita)、水螅只有2类POU。在本研究分析的刺胞动物中,安朵仙水母的POU-1,-6亚型的核苷酸多态性最高;安朵仙水母与指形鹿角珊瑚(Acropora digitifera)、星状海葵的POU-2/3/5亚型的序列多样性较其他钵水母更高。另外,3种钵水母与水螅粘附蛋白的比较结果表明,安朵仙水母具有巴布亚硝水母和海蜇所不具有的粘附相关鼠李糖结合凝集素和一类抗氧化活性物质铁螯合物还原酶。综上所述,安朵仙水母具有更多POU编码基因和复杂POU亚型,以及具有粘附相关凝集素和还原酶的编码基因,可能是与安朵仙水母倒立附着生活方式相关的关键遗传信息。     

GF-2在城市绿地分类及景观格局度量中的应用

城市植被是维护城市生态系统平衡的重要组成元素,不同类型的植被具有不同的生态服务效益,然而基于植被精细分类的城市绿地景观格局度量研究较少。因此本文以北京城市副中心为研究区,使用夏、冬两季GF-2卫星遥感影像,基于随机森林特征优选和面向对象分类,划分了植被类型,并在此基础上使用景观指数法和移动窗口法分别度量了功能区尺度和栅格尺度绿地景观特征。研究结果表明：针对GF-2数据,使用多尺度分割后影像对象的光谱、纹理特征可以有效地提取植被信息;不同时相的影像能反映各类型植被的物候特征,相比于单时相数据,其分类精度更高,达到了87.7%;各功能区绿地景观格局特征差异较大,城市绿心拥有最丰富的植被且分布集中,商业区植被覆盖度和多样性均较低,绿地景观破碎;北京城市副中心景观多样性及各类型植被分布特征的空间差异显著,当前副中心绿地景观格局已形成规划的基本轮廓,但城市绿心和老城区公共绿地的建设仍有不足。研究探明了北京城市副中心绿地建设现状,证明了国产GF-2卫星在城市生态环境监测中具有较高的应用价值,有助于推动GF-2在城市生态环境领域的应用,并为副中心建设中的绿地系统监测和优化提供参考。     

Mapping and assessing crop diversity in the irrigated Fergana Valley,Uzbekistan

Crop diversity (e.g. the number of agricultural crop types and the level of evenness in area distribution) in the agricultural systems of arid Central Asia has recently been increased mainly to achieve food security of the rural population, however, not throughout the irrigation system. Site-specific factors that promote or hamper crop diversification after the dissolvent of the Soviet Union have hardly been assessed yet. While tapping the potential of remote sensing, the objective was to map and explain spatial patterns of current crop diversity by the example of the irrigated agricultural landscapes of the Fergana Valley, Uzbekistan. Multi-temporal Landsat and RapidEye satellite data formed the basis for creating annual and multi-annual crop maps for 2010–2012 while using supervised classifications. Applying the Simpson index of diversity (SID) to circular buffers with radii of 1.5 and 5 km elucidated the spatial distribution of crop diversity at both the local and landscape spatial scales. A variable importance analysis, rooted in the conditional forest algorithm, investigated potential environmental and socio-economic drivers of the spatial patterns of crop diversity. Overall accuracy of the annual crop maps ranged from 0.84 to 0.86 whilst the SID varied between 0.1 and 0.85. The findings confirmed the existence of areas under monocultures as well as of crop diverse patches. Higher crop diversity occurred in the more distal parts of the irrigation system and sparsely settled areas, especially due to orchards. In contrast, in water-secure and densely settled areas, cotton-wheat rotations dominated due to the state interventions in crop cultivation. Distances to irrigation infrastructure, settlements and the road network influenced crop diversity the most. Spatial explicit information on crop diversity per se has the potential to support policymaking and spatial planning towards crop diversification. Driver analysis as exemplified at the study region in Uzbekistan can help reaching the declared policy to increase crop diversity throughout the country and even beyond.     

Effects of Enclosure on Plant and Soil Restoration in the Junggar Desert

Enclosure is commonly used in the restoration of degraded grasslands. However, the effects of enclosure on grassland plant and soil restoration remain controversial, particularly in deserts. To assess the effects of enclosure on desert plants and soil properties, using high throughput sequencing, the differences between plants and soil were systematically analyzed before and after enclosure construction. The soil organic carbon, total nitrogen and total phosphorus contents of the three desert flora increased and decreased, but the difference was not significant; enclosure increased plant height, coverage, aboveground biomass, and species richness by 58.99%, 59.35%, 33.29%, and 51.21%, respectively, in a Seriphidium transiliense formation; by 15.49%, 33.52%, 20.85%, and 5.13%, respectively, in a Haloxylon persicum formation; and by 83.80%, 31.51%, 76.66% and 33.33%, respectively, in an Anabasis salsa formation. For soil bacteria, enclosure significantly increased the average number of operational taxonomic units and Shannon-Wiener index by 12.74% and 2.92%, respectively, under S. transiliense formation and by 17.08% and 3.17%, respectively, under H. persicum formation. However, enclosure had no significant effect on the average number of operational taxonomic units or Shannon-Wiener index under A. salsa formation. Enclosure significantly increased desert plants, soil bacterial diversity, and desert plant community productivity; however, the increase in soil nutrient content was not significant. These results demonstrate that enclosure is effective for restoring desert ecosystems but may have little effect on the soil nutrient content.     

荒漠土壤微生物群落结构特征研究进展

荒漠生态系统占地球陆地面积三分之一,是地球化学循环中的重要部分。荒漠干旱高温、缺乏植被、UV辐射强,曾被认为是没有生命的地方。然而在这恶劣环境中却蕴含有大量的微生物资源,尤其是荒漠土壤富集了大量微生物。微生物参与和主导整个荒漠生态系统地球生物化学循环,对于调节重要生态过程、修复和稳定荒漠生态系统起到重要作用,对其的研究生态学意义突出。本文综述了国内外对荒漠土壤微生物群落结构特征、群落功能多样性以及微生物群落、微生物与植物、微生物与环境之间相互关系的研究现状,旨在充分了解荒漠土壤微生物多样性研究,总结对荒漠微生物生态认识的不足,为荒漠微生物生态研究方向提供参考。     

基于创新的城市化——中国270个地级及以上城市数据的实证分析

传统城市化概念已难以解释全球范围内出现的新兴城市化模式,而“创新”是解读城市化新变化的重要概念。从“流的空间”视角,提出了基于创新的城市化概念。在此基础上,选取中国地级及以上270个城市作为研究的空间单元,依据第六次全国人口普查资料,分析了中国高技能迁移人口和城市文化多元性的空间分布特征,采用空间杜宾模型方法,构建城市创新空间回归模型,测度了中国高技能迁移人口规模和城市文化多元性水平对城市创新产出的影响,验证了基于创新的城市化概念。研究表明：① 基于创新的城市化概念顺应知识经济发展的背景,强调高技能劳动力向城市的迁移过程,促进城市职能与景观发生变化,城市知识活动与行为的扩展,可以很好的解释城市发展的新动力、新机制和新的创新景观。② 基于高技能劳动力迁移的创新的城市化是城市发展的重要动力,对中国的沿海及省会城市的城市化发展有重要作用。③ 中国国内高技能劳动力迁移规模和城市文化多元性水平均对城市创新产出具有正效应。在其他条件不变的情形下,城市高技能迁移人口数量每增加10%,直接带来的城市创新产出增加3%;城市文化多元性水平每提高10%,直接带来的城市创新产出增加2%。研究在理论上加深了对区域创新系统开放性和动态性的理解与认识,为知识经济下城市化和城市发展政策的制定提供了重要理论依据。     

A comparison of results from geomorphological diversity evaluation methods in the Polish Lowland (Toruń Basin and Chełmno Lakeland)

Geomorphological diversity is part of geodiversity. Study and evaluation of geodiversity, including geomorphological diversity, is often conducted in uplands and mountains, despite the fact that lowland areas are of equal importance. This paper evaluates geomorphological diversity in a small area of the Polish Lowland, using a variety of methods that have been applied in recent times for evaluating geodiversity, and presents the results on maps. By comparing these maps and analyzing the correlation coefficients of the results obtained, it was possible to identify the two methods that were best suited to indicating areas with the greatest geomorphological diversity in the lowlands. These two methods are least affected by the choice of elementary fields and data classification methods applied. The study identified the two areas with the greatest relief diversity and showed that they distinctly differ from one another. They demonstrate the major influence of processes, not only on the topographic parameters and landform types, but above all on identifying and defining total geomorphological diversity. These methods, which can be used to identify the areas with the greatest total geomorphological diversity, could readily be used in applied studies relating to abiotic ecosystem services and landscape management.     

What can we learn about changing ethnic diversity from the distributions of mixed-race individuals?

It is hypothesized that self-defined mixed-race persons live in residentially mixed areas in the largest metropolitan areas in California. The hypothesis is tested by examining the distribution of mixed-race persons among ethnically and racially diverse and nondiverse neighborhoods in the San Francisco and Los Angeles Metropolitan Areas. The research confirmed that mixed-race individuals are more likely to live in areas with ethnic diversity and that the tendency is greater for the mixed-race population in the San Francisco–Oakland Metropolitan Areas than in the Los Angeles Metropolitan Area. Mixed-race individuals live in neighborhoods which are diverse with mixes of all four major ethnic and racial groups, and in “well-off” (but not the most affluent) neighborhoods. The study also shows that the mixed-race population is youthful. The association of mixed-race individuals and racially integrated neighborhoods will have important implications for the evolving nature of spatial integration in California specifically, and the United States more generally.