Particulate peptidoglycan in seawater determined by the silkworm larvae plasma (SLP) assay

Peptidoglycan (PG) is a biopolymer found exclusively in the cell wall of bacteria. Recent chemical analysis of particulate organic matter suggests that a major amount of the muramic acid, an amino sugar present only in PG, could not be accounted for in terms of bacterial cells (Benner and Kaiser, 2003); however, data on particulate PG is quite sparse. In the present study, conducted in 1996, the PG was examined at 5 sampling sites in the northwestern Pacific Ocean, and in natural seawater cultures. Particulate PG, which was concentrated using a 96-well filtration plate equipped with Durapore filters (pore size, 0.22 μm), was measured by the silkworm larvae plasma (SLP) assay. The PG concentration generally decreased with depth and correlated significantly with bacterial abundance throughout the entire water column. However, the ratio of particulate PG to bacterial abundance varied with depth. The average ratio was 0.61 ± 0.53 (average ± SD, n = 40) between 50 and 2000 m, which agreed with the bacterial cellular PG content from 0.63 to 1.1 fg cell⁻¹ obtained in seawater cultures. On the other hand, the ratios of PG to bacteria from the surface to 50 m (3.7 ± 2.6, n = 29) and below 2,000 m (2.1 ± 1.7, n = 7) were significantly higher than that between 50 and 2,000 m. These results may suggest that, in the surface and deep layers, a significant fraction of particulate PG was present in bacterial detritus, whereas this fraction was reduced in the middle layer.     

双层局部开孔板沉箱对波浪反射的理论研究

提出了一种用于研究由双层开孔板和一个不透水后板的开孔结构对斜向波反射率的理论分析方法。整个流域被分成三个子域，在每个子域内应用特征函数展开法以得到该域内包含未知展开系数的势函数的表达式，在速度势的展开中，考虑了非传播模态波浪的影响。通过匹配开孔板处的边界条件可以求解待定的展开系数，继而求解双层开孔板防波堤结构对斜向波的反射率。数值计算结果与试验结果进行了比较，符合较好。并进一步讨论了几个重要因素对反射系数的影响。     

Flow fields in reflected waves at a sloped sea wall

Wave run-up, and flow visualization experiments were conducted with a 1:2 sloped sea wall model. The visualization experiments gave an overview of flow fields in reflected, non-breaking conditions. Maximum particle velocities were found to be significantly smaller than suggested in the literature. Downrush produced a fast sheet flow, extending down to the toe of the sea wall. This created a ‘reverse’ breaker during the retreat of the initially non-breaking wave, which explains the high-energy dissipation rates for non-breaking waves reported in the literature. Embankments may therefore be exposed to wave impact pressures in areas up to 1.18H₀ below MWL.     

Stability of waterfront retaining wall subjected to pseudo-static earthquake forces

Waterfront retaining walls supporting dry backfill are subjected to hydrostatic pressure on upstream face and earth pressure on the downstream face. Under seismic conditions, if such a wall retains a submerged backfill, additional hydrodynamic pressures are generated. This paper pertains to a study in which the effect of earthquakes along with the hydrodynamic pressure including inertial forces on such a retaining wall is observed. The hydrodynamic pressure is calculated using Westergaard's approach, while the earth pressure is calculated using Mononobe-Okabe's pseudo-static analysis. It is observed that when the horizontal seismic acceleration coefficient is increased from 0 to 0.2, there is a 57% decrease in the factor of safety of the retaining wall in sliding mode. For investigating the effect of different parameters, a parametric study is also done. It is observed that if φ is increased from 30° to 35°, there is an increase in the factor of safety in the sliding mode by 20.4%. Similar observations were made for other parameters as well. Comparison of results obtained from the present approach with [Ebeling, R.M., Morrison Jr, E.E., 1992. The seismic design of waterfront retaining structures. US Army Technical Report ITL-92-11. Washington DC] reveal that the factor of safety for static condition (k_h=0), calculated by both the approaches, is 1.60 while for an earthquake with k_h=0.2, they differ by 22.5% due to the consideration of wall inertia in the present study.     

长期非平稳荷载调谐质量减振阻尼器优化设计

研究外荷载为长期非平稳随机过程。考虑长期荷载的特性 ,采用 1个概率谱密度函数来反映长期非平稳随机荷载及其特征 ;概率谱密度函数是基于大量的一般谱密度函数的统计特性获得。以延长结构的抗疲劳使用寿命为目标函数 ,提出了调谐质量减振阻尼器的优化设计方法 ,这在实际工程中有着极为广阔的应用前景。本文旨在从理论上发展长期非平稳随机荷载作用下调谐质量减振阻尼器的优化设计方法 ;文中采用长期波浪实测数据 ,给出了 1个数值算例说明整个设计过程。     

Instrumentation and Numerical Analysis of Cylindrical Diaphragm Wall Movement During Deep Excavation at Coastal Area

The lateral deflection of a cylindrical diaphragm wall and the associated ground movement induced by deep excavation are analyzed by performing site instrumentations and numerical analyses in the coastal area of Korea. Wall lateral deflection, rebar stress, and pore water pressure were measured and analyzed in eight directions. Variations of soil properties with the decrease of confining pressure are compared by performing various in situ tests before ad after excavation. To calculate the wall lateral deflection accurately, the effects of small strain nonlinearity, confining pressure, and the hysteresis loading/unloading loop developed during excavation are considered in the proposed numerical analysis. By comparing numerical results with measured ones, the importances of considering small strain nonlinearity and confining pressure reduction in the nonlinear (FEM) are emphasized. Also, the effects of wall stiffness on the performance of cylindrical diaphragm walls are studied for future similar excavation in the coastal area.     

一种基于双重距离的空间聚类方法

传统聚类方法大都是基于空间位置或非空间属性的相似性来进行聚类,分裂了空间要素固有的二重特性,从而导致了许多实际应用中空间聚类结果难以同时满足空间位置毗邻和非空间属性相近。然而,兼顾两者特性的空间聚类方法又存在算法复杂、结果不确定以及不易扩展等问题。为此,本文通过引入直接可达和相连概念,提出了一种基于双重距离的空间聚类方法,并给出了基于双重距离空间聚类的算法,分析了算法的复杂度。通过实验进一步验证了基于双重距离空间聚类算法不仅能发现任意形状的类簇,而且具有很好的抗噪性。     

地下连续墙治理地下水污染

介绍一种治理已被严重污染的地下水的方法。即修建一道底部深入到不透水岩层的地下墙,把污染物封闭在由底部不透水岩层和周围地下防渗墙组成的“地下盆”中,降低周围地下水中有害物质的浓度,然后再治理封闭圈内的污染物,以达到彻底根治地下水污染的目的。     

硫铅酸盐“S”型瞬凝水泥干法护壁堵漏技术研究

介绍了硫铝酸盐“Ｓ”型瞬凝水泥干法者漏时使用的配方,阐述了输送工具和扫孔钻具的设计以及灌注工艺技术。     

土钉墙与预应力锚杆联合支护技术

北京市深基坑工程中土钉墙支护技术应用比较广泛，对于边坡变形要求严格的基坑，采用土钉墙与预应力锚杆联合支护技术可有效控制变形，结合工程实例，介绍了该技术的设计思路及应用过程，说明了该技术的适用性，并就施工中遇到的问题进行分析，给出相应的处理措施。