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Sequence analysis and quantitative detection of Norwalk-like viruses in cultured oysters of China

We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commer-cial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The re-suits showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient's fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×108, 1.25×108 and 4.7×101 respectively. The detecting limit of NLVs was 1×101 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future.     

Evaluation of daily precipitation in China from ECMWF and NCEP reanalyses

ＩＮＴＲＯＤＵＣＴＩＯＮＷｅａｔｈｅｒｆｏｒｅｃａｓｔｉｎｇａｓａｍａｊｏｒｓｕｂｊｅｃｔｉｎａｔｍｏｓｐｈｅｒｉｃｓｃｉｅｎｃｅｈａｓｄｅｖｅｌｏｐｅｄｓｉｎｃｅｔｈｅ 1 950’ｓｉｎｔｏａｍｏｄｅｒｎｓｃｉｅｎｃｅ .Ｎｕｍｅｒｉｃａｌｗｅａｔｈｅｒｆｏｒｅｃａｓｔｍｏｄｅｌｓａｒｅｅｘｔｅｎｓｉｖｅｌｙａｎｄｆｒｅｑｕｅｎｔｌｙｕｓｅｄｔｏｃｈｅｃｋｔｈｅｔｈｅｏｒｉｅｓ,ｒｕｌｅｏｕｔｔｈｅｏｌｄｉｎｃｏｒｒｅｃｔｏｎｅｓａｎｄｐｒｅｓｅｎｔｎｅｗｉｄｅａｓ,ａｎｄｓｕｇｇｅｓｔｍｅｔｈｏｄｓ…     

GNSS变形监测网短基线时间序列噪声特性分析

利用时间跨度为5 a的GNSS短基线时间序列对噪声特性进行分析,发现长周期噪声分量(随机游走噪声)。选取最优噪声模型,评估不同噪声模型对测站周期振幅和线性速度估值的影响。结果表明,短基线时间序列中有色噪声应顾及闪烁噪声和随机游走噪声,对于表现出随机游走噪声的分量,可能与测站的真实运动有关;假设只有白噪声时求得的速度估值与最优噪声模型下求得的速度估值存在0.4～0.6 mm/a的偏差,对周期振幅的影响可以忽略。     

基于GPT2w模型化加权平均温度反演可降水量

提出一种基于GPT2w模型化加权平均温度反演大气可降水量的方法,并分析附加系统偏差改正的模型化加权平均温度对可降水量的影响。结果表明,基于GPT2w模型化加权平均温度反演的大气可降水量的精度与基于Bevis公式计算的加权平均温度反演的大气可降水量的精度相当;对GPT2w模型化加权平均温度进行系统偏差改正后,大气可降水量的精度有一定改善,但改善率不到1%。     

1978年以来广州市居民职住地选择行为时空特征与影响因素的代际差异

1978年以来,中国体制改革重塑了个体日常时空行为及其在生命过程中的活动轨迹,但鲜有文献解析长时间序列下不同代际群体对职住政策调整做出的响应及其代际差异。基于时间地理学视角与代际差异理论,将广州市微观个体职住地变动历程与代际问题相结合,利用问卷调查分析1988年和1998年居民职住制度改革前后,不同代际居民职住地变迁行为时空特征及其影响因素的结构性差异。结果发现：① 在居住地和就业地变迁方向方面,各代际居民住房迁出地主要集中于老城区,就业地变迁围绕两个城市中心,形成由老城核心地域向内城、近郊扩散的变迁过程。② 职住地距离变化方面,居民平均职住距离由1988年之前的4.91 km增至1998年之后的6.46 km,20世纪90年代出生居民（简称90后,下同）的职住分离度大于其他群体。③ 在职住地变迁率方面,住房商品化和就业社会化极大地增加了居民职住地选择自由度,60后和70后在1998年之后的迁居率分别为113.16%与112.33%,就业地变迁率分别为148.68%与197.26%,二者都远高于住房改革前的比例。④ 驻留时长方面,职住自由化使得驻留时长明显缩短。60后变化最为明显,该群体在一个居住地的居住时长由1988年以前的14.43年缩至1998年以后的5.43年,就业地驻留时长由12.43年降至3.95年;80后与90后在1998年以后的职住地驻留时长明显较短,70后相对较长。⑤ 能力、组合及权威制约中的房价、婚姻状况、学历、福利分房及下岗等因素对职住地变迁表现出明显代际差异,因子女上学及同事关系等组合制约对就业地变迁无显著群体差别。     

A surface network based method for studying urban hierarchies by night time light remote sensing data

Urban hierarchies are closely related to economic growth, urban planning and sustainable urban development. Due to the limited availability of reliable statistical data at fine scales, most existing studies on urban hierarchy characterization failed to capture the detailed urban spatial structure information. Previous studies have demonstrated that night time light data are correlated with many urban socio-economic indicators and hence can be used to characterize urban hierarchies. This paper presents a novel method for studying urban hierarchies from night time light data. Night time light data were first conceptualized as continuous mathematical surfaces, termed night time light surfaces. From the morphology of these surfaces the corresponding surface networks were derived. Hereafter, a night time light intensity (NTLI) graph was defined to describe the morphology of the surface network. Then, structural similarity between the night time light surfaces of any two different cities was calculated via a threshold-based maximum common induced graph searching algorithm. Finally, urban hierarchies were defined on the basis of the structural similarities between different cities. Using the 2015 annual NPP-VIIRS night time light data, the urban hierarchies of 32 major cities in China were successfully examined. The results are highly consistent with the reference urban hierarchies.     

Stochastic bivariate time series models of waves in the North Sea and their application in simulation-based design

In this paper, we present and evaluate three long-term wave models for application in simulation-based design of ships and marine structures. Designers and researchers often rely on historical weather data as a source for ocean area characteristics based on hindcast datasets or in-situ measurements. The limited access and size of historical datasets reduces repeatability of simulations and analyses, making it difficult to assess the sampling variability of performance and loads on marine vessels and structures. Markov, VAR and VARMA wave models, producing independent long-term time series of significant wave height (H_s) and spectral peak period (T_p), is presented as possible solutions to this problem. The models are tested and compared by addressing how the models affect interpretation of design concepts and the ability to replicate statistical and physical characteristics of the wave process. Our results show that the VAR and VARMA models perform sufficiently in describing design performance, but does not capture the physical process fully. The Markov model is found to perform worst of the tested models in the applied tests, especially for measures covering several consecutive sea states.     

Experimental study on a sampling technique based on a freeze-sediments valve for deep-sea microorganism

Deep-sea microorganism research has become a hot topic in life science, owing to its special value in high pressure and low temperature environment. If not kept the in-situ low temperature and high pressure, it will cause the microorganism to lose its activity and lead to a waste of resources. A freeze-sediments valve presents a promising solution to maintain the low temperature and high pressure in situ, improve the success rate of sampling and shorten the sampling time. A series of experiments were conducted to characterize the freeze-sediments valve, including the sampling time without leakage, adaptation to different sediments and the effect of the length, etc. Most important of all, temperature change of sediments is observed in all experiments. Experimental results indicated that the freeze-sediments valve was formed in 5 min without leakage and the freeze-sediments valve could be applied to different sediments without limitations on sediments type and mineral content. Considering the pressure retaining capacity and formation time of freeze-sediments valve, it was found that the freeze-sediments valve with a length of 60–80 mm has good pressure retaining capacity whose value was 41.3–48.4 Mpa and short formation time which was 5 min. Precooling the sampler could be used to shorten the sampling time in the actual environment application. This sampling technique can play a vital role in maintaining in-situ low temperature at −2 to −4 ° Celsius in all the experiments. According to the tests, the sampling technique based on the freeze-sediments valve had the potential to solve the problem of keeping in-situ temperature, low sampling success rate and long sampling time in the future.     

Ekman模型时间变化风应力系数的伴随参数估计

本文基于时间分布参数设置,利用伴随同化方法,反演了Ekman模型中随时间变化的风应力拖曳系数,并在孪生实验和实际实验中对该方法进行了验证。在孪生实验中,研究了参数反演结果对不同影响因素的响应,包括:风速分布、风应力系数分布、风应力系数初始猜测值、风应力系数独立变量个数、观测数据误差和观测的深度。孪生实验结果验证了伴随同化方法反演Ekman模型中时变风应力系数的有效性,具体包括如下五个方面结论:1)不同风速分布下均能成功反演出不同风应力拖曳系数分布; 2)反演结果对初始猜测值较为敏感,风应力系数初始猜测值越接近给定值,反演结果越好;3)风应力系数独立点个数的选取会显著影响反演结果,合理的选择有利于提高反演效率及减小观测数据误差;4)观测误差能够影响反演结果,观测数据误差在20%以下时能取得合理的反演结果; 5)反演结果对观测数据的表层和次表层流速更为敏感,这是由Ekman流的物理性质决定的。实际实验,利用百慕大锚系试验平台的风速和流速数据,去除周期性潮流和地转流成分后得到Ekman流成分,并作为观测输入到该同化模型,反演出了适用于该区域和该时段的随时间变化的风应力系数。通过比较模拟流速和观测流速,证明利用伴随同化方法能从实测数据中反演出合理的时变风应力系数,对于海洋模型风应力系数的确定是一项有益的尝试。