鼠尾藻(Sargassum thunbergii)对邻苯二甲酸二甲酯(DMP)胁迫的响应

邻苯二甲酸二甲酯(dimethyl phthalate,DMP)是近海环境有机污染物之一,不仅对海洋生物造成毒害,且能在生物体内富集和放大,对海洋生态系统形成严重威胁。本文以鼠尾藻(Sargassum thunbergii)为研究对象,通过测定比生长速率、叶绿素a含量、光合和呼吸速率、光合基因rbcL转录水平上的相对表达量等指标,研究了鼠尾藻对不同浓度DMP的响应。结果表明,低浓度的DMP(0.lmg/L)短时间内(≤15d)能促进鼠尾藻的生长和叶绿素a的合成,并使藻体的光合作用增强,诱导光合基因rbcL的表达,而在高浓度DMP(≥0.3mg/L)的作用下,鼠尾藻的生长速率、光合和呼吸速率、rbcL基因的表达量均随着暴露时间的延长呈明显下降趋势,且DMP浓度越高,降幅越大。以上研究结果对了解DMP对鼠尾藻胁迫的影响机制奠定了基础,为保护海洋经济藻类的养殖环境提供了理论依据。     

南海东北部分粒级叶绿素a和超微型光合生物的周日变化

分别于2014年春、秋和2015年夏3个季节对南海东北部A站位(118°E,21.5°N)分粒级叶绿素a浓度和超微型光合生物(原绿球藻、聚球藻和超微型真核藻类)细胞丰度的昼夜变化进行了24 h时间序列连续观测和分析。通过萃取荧光法分析叶绿素a浓度,发现叶绿素a浓度呈现明显的昼夜变化,春季正午最高,秋季和夏季基本变化趋势为白天升高,夜晚降低;而因夏季中午的光抑制作用,叶绿素a的浓度相对较低。超微型光合生物(0.2~3μm)对总叶绿素a的贡献最高(71.49%),小型浮游植物(20μm)贡献率最低(10.41%)。通过流式细胞技术检测到3个超微型光合生物类群;其中,原绿球藻为优势类群,最大细胞丰度达1.05×10~5cells/m L,其次是聚球藻,超微型真核藻类的细胞丰度最低,但由于其单位细胞内的叶绿素a含量高,所以可能对叶绿素a的贡献最大。聚球藻丰度基本上白天下降,傍晚到午夜上升;秋季和夏季,超微型真核藻类的丰度白天高,夜晚低,而春季则相反;原绿球藻在秋季和春季的昼夜变化规律和超微型真核藻类相似。在多种因素共同影响下,光照是调控叶绿素a浓度和超微型光合生物丰度昼夜变化的一个关键因素。季节变化上,原绿球藻的细胞丰度季节间没有统计学差异(P0.05),聚球藻的季节变化为秋夏春,超微型真核藻类的季节变化规律和聚球藻相反。     

外源硒对罗布麻幼苗生长及光合作用的影响

硒是植物生长发育的必需元素,能促进植物生长。采用盆栽试验的方法,研究了施用不同浓度的外源硒对一年生罗布麻幼苗生长及叶绿素荧光参数的影响。研究结果表明：根施（R）和叶面喷施（L）两种施硒方式下,硒浓度与罗布麻一年生幼苗生长及叶绿素荧光参数有极显著相关性,表现为,一定浓度范围内（0～1.5 mg·kg^-1）,随着硒浓度的增加,罗布麻茎干重、叶干重、株高、茎粗、主叶对数和侧枝数均有有不同程度的上升;Fv/Fm、Fv/Fo、Ψo、φEo、PIABS、均随着施硒浓度的增加而增加;V_K、V_J、V_I均随着施硒浓度的增加而逐渐下降,S_m逐渐上升;说明施硒促进了罗布麻叶PSII的活性,提高了PSII反应中心的开放程度,增大其光能利用率。但随着供应浓度的进一步提高,各项生长及荧光指标有所降低,因此过量的硒供应对于罗布麻生长不利,而且有益与有害之间的范围很小,具体罗布麻栽培中要严格控制硒用量在1～3 mg·kg^-1范围内。搜索 #SrsZzqBAngjSTips { Z-INDEX: 999999999; POSITION: absolute; WIDTH: 56px; HEIGHT: 24px; LEFT: 1342177.27em } #SrsZzqBAngjSTips A { POSITION: relative; LINE-HEIGHT: 24px; MARGIN: -32px 0px 0px; PADDING-LEFT: 23px; WIDTH: auto; DISPLAY: block; BACKGROUND: url(http://mat1.gtimg.com/www/sogou/sogou_tips_v1.png) no-repeat 0px 0px; HEIGHT: 24px; COLOR: #000; FONT-SIZE: 12px; TEXT-DECORATION: none } #SrsZzqBAngjSTips A:hover { BACKGROUND-POSITION: 0px -34px; COLOR: #45a1ea }     

Seston transport and deposition in Pelorus sound,south Island,New Zealand

Transport of seston (suspended sediment) in Pelorus Sound is controlled by tides and freshwater inflow. During high freshwater inflow, a moderately stratified estuarine circulation may be superimposed on the tidal circulation, but the latter dominates and transports seston seawards and landwards with the ebb and flood phases respectively. With extreme freshwater inflow, the estuarine circulation gains impetus and most seston is rapidly transported seaward in the low saline surface layer.

Irrespective of circulation there is a persistent trend in seston concentrations. Highest values occur at the sound's head because of the influence of nearby Pelorus and Kaituna Rivers and because of resuspension of bottom sediment by strong tidal currents. Seston concentrations wane along the sound until near the entrance, where values increase as a result of greater production of biogenic seston and because additional seston is brought in from Cook Strait with the flood tide. This trend parallels variability in the thicknesses of muddy bottom sediments. Muds are thick at the head where an extensive delta extends from the river mouths; muds gradually thin seaward and then thicken markedly in the vicinity of the sound entrance.

Seston weight and composition patterns and 3.5 kHz seismic profiles indicate Pelorus Sound acts as a double‐ended sediment trap. The upper reaches receive and retain river‐derived seston, whereas the sound entrance traps seston derived from Cook Strait. This situation appears to hold for both high and extremely high influxes of sediment.     

Northern Adriatic phytoplankton response to short Po River discharge pulses during summer stratified conditions

A 275‐km‐long transversel Northern Adriatic profile from the mouth of the Po River (Italian Adriatic coast) to the Kvarner region (Croatian coastal island area) was investigated in three successive case studies in August 2008, 2009 and 2010. The short Po River pulses in August result in the surface advection of riverine water, nutrients and phytoplankton from the western to the eastern side of the Adriatic. This surface spreading exhibits inter‐annual variability depending on the riverine discharge in the preceding period. The Po River discharge pulse in August 2010 in particular resulted in an extraordinary tongue‐like advection of riverine water, nutrients, and phytoplankton towards the Eastern Adriatic coast. The phenomenon was detected using both satellite imagery and classical oceanographic measurements. In the advective water, toxic dinoflagellates were most abundant in August 2010, when the influence of the Po was greatest.     

Comparison in copper accumulation and physiological responses of Gracilaria lemaneiformis and G. lichenoides (Rhodophyceae)

Heavy metal pollution has become a worldwide problem in aquaculture. We studied copper (Cu2+ ) accumulation and physiological responses of two red algae Gracilaria lemaneiformis and Gracilaria lichenoides from China under Cu2+ exposure of 0-500 μg/L in concentration. Compared with G. lemaneiformis, G. lichenoides was more capable in accumulating Cu2+ , specifically, more Cu2+ on extracellular side (cell wall) than on intracellular side (cytoplasm) and in cell organelles (especially chloroplast, cell nucleus, mitochondria, and ribosome). In addition, G. lichenoides contained more insoluble polysaccharide in cell wall, which might promote the extracellular Cu2+ -binding as an efficient barrier against metal toxicity. Conversely, G. lemaneiformis was more vulnerable than G. lichenoides to Cu 2+ toxin for decreases in growth, pigment (chlorophyll a, chlorophyll b, phycobiliprotein, and β-carotene) content, and photosynthetic activity. Moreover, more serious oxidative damages in G. lemaneiformis than in G. lichenoides, in accumulation of reactive oxidative species and malondialdehyde, and in electrolyte leakage, because of lower antioxidant enzyme (superoxide dismutase and glutathione reductase) activities. Therefore, G. lichenoides was less susceptible to Cu2+ stress than G. lemaneiformis.     

The preliminary study of the high chlorophyll in the central Bohai Sea in summer

In the central Bohai Sea, both historical data and in situ observations show that the distribution of high chlorophyll concentration is consistent with warm water. The previous studies confirm that the warm water is caused by a joint effect of wave and tidal mixing. In this paper, the authors have coupled the Princeton ocean model (POM) with a biochemical model to further investigate the formation of the high chlorophyll concentration. When the wave mixing is excluded, the water temperature is lower and the chlorophyll concentration is also lower than the observation. However, if the wave mixing is included, strong wave mixing in the upper layer brings the warm water downward and nutrients upward so that both temperature and nutrients tend to be uniformly high from the surface to the bottom. This provides an environment which is favorable for the growth of the phytoplankton. Therefore the chlorophyll concentration, as a symbol of phytoplankton, shows high value in the central Bohai Sea. In conclusion, the wave mixing plays an important role in the formation of high chlorophyll concentration in the central Bohai Sea.     

胶州湾大沽河口潮间带沉积物中叶绿素和有机质含量的季节变化

为了解胶州湾大沽河口潮间带沉积物中底栖微藻和有机质含量的季节变化,于2010年4月至2011年2月,分4次(春、夏、秋、冬)测定分析了沉积物中的叶绿素含量和有机质含量,并分析了沉积物的粒度组成及现场温度。结果表明,表层(0～2cm)沉积物叶绿素a含量为0.42～6.06mg/kg,有机质含量为0.48%～3.71%;次表层(2～5cm)叶绿素a含量为0.13～3.53mg/kg,有机碳质量为0.31%～3.78%;深层(5～8cm)叶绿素a含量为0.14～1.95mg/kg,有机质含量为0.47%～3.72%。经统计检验和Pearson相关性分析得出,胶州湾大沽河口沉积物中的叶绿素a含量春冬两季最高,夏季和秋季最低;叶绿素a含量与深度和温度呈负相关;与所处潮带、有机质含量、粉砂黏土含量在本实验中均无显著的相关性;有机质含量与季节关系不大,高潮带最高,其含量与粉砂黏土含量呈正相关,与叶绿素含量、深度和温度关系不明显。     

Preliminary investigations on Arctic microalgae by joint aDDlication of fluorescent instruments

In vivo fluorescence has a wide application in analyzing microalgae, including assessing phytoplankton biomass, rates of primary production and physiological status. This study describes a preliminary investigation on the joint application of the three kinds of fluorescence analysis in the physiological study of microalgae. Flow cytometry and fluorescence spectrometry were used to obtain the in vivo static fluorescence information of pigments, and a Pulsed-Amplitude-Modulation chlorophyll fluorometer was used to detect the dynamic fluorescence of chlorophyll. The validity of the joint application was proved by analyzing two labora- tory cultured Arctic microalgae, Pseudo-nitzschia delicatissima （Bacillariophyceae） and Thalassiosira sp. The higher value of minimum fluorescence yield in dark-adapted state （Fo）, actual photochemical efficiency of PSll （ФPSII）, and electron transport rate （ETR） exhibited positive results in a higher cell abundance and chlorophyll a content of P. delicatissima; whereas higher fl-carotene content of Thalassiosira sp. played an important role in the protection of photosynthesis.     

两种盐生植物幼苗在NaCl处理下对昼夜温差变化的叶绿素荧光响应

以囊果碱蓬(Suaeda physophora Pall.)、梭梭(Haloxylon ammodendron (C.A.Mey.) Bunge)两种植物幼苗为实验材料,研究了不同NaCl浓度处理下,一次昼夜温差（温差为27 ℃）变化对两种植物幼苗叶绿素荧光参数变化的影响。结果表明:①在NaCl胁迫下,同期囊果碱蓬的PSⅡ潜在活性Fv/Fo和最大光化学量子产量Fv/Fm降低幅度较小,而梭梭则有较明显降低,而且囊果碱蓬显著高于后者;同期光合量子产量Yield和表观光合电子传递速率ETR均以囊果碱蓬最大并显著高于梭梭,说明囊果碱蓬较梭梭具有强的对盐分和较大的昼夜温差变化的忍耐能力,其机体在盐分和较大昼夜温差变化下,并未受到较大的损伤,而梭梭的光合器官在相同的条件下却受到了较大的损伤,证明了囊果碱蓬更适合生长于同时具有盐渍和较大昼夜温差的地区。②通过实验发现梭梭的Yield值和ETR值到下午基本上能恢复到早上的水平,说明了其在前期的胁迫条件下,虽然光合器官上受到了破坏,但仍然能够通过光合调节适应环境的变化,以保持机体的活性。③囊果碱蓬在本实验条件下,光合器官虽然没有受到损伤,但200 mmol\5L-1 NaCl抑制了其光合能力。