高通量测序分析北仑河口红树林潮间带底栖真核生物分布

为了解红树林不同潮位沉积物中底栖真核生物群落分布,基于18S rRNA基因采用高通量测序方法分析了广西北仑河口陆缘、林中和海缘3个潮位红树林沉积物中底栖生物群落结构。结果表明,北仑河口潮间带红树林沉积物中底栖生物多样性丰富,Shannon-Wiener指数变化范围在6. 08～6. 73之间; PCA分析表明潮间带中底栖生物群落差异较大,陆缘红树林中扁形动物、节肢动物和软体动物相对丰度较高,林中区域中纤毛虫、环节动物和轮虫相对丰度较高,海缘红树林中硅藻相对丰度较高;红树林中主要OTUs有桡足类的太平洋纺锤水蚤(Acartia pacifica)、硅藻类的海链藻(Thalassiosira sp.)、纤毛虫类的前管虫(Prorodon teres)、多毛类的小头虫(Capitella sp.)。高通量测序方法能较全面反映红树林区微型/小型底栖生物群落,研究结果为丰富红树林底栖生物群落研究和解析底栖生物在红树林生态系统发挥的作用提供基础数据。     

用于海洋沉积物中厌氧氨氧化细菌分子生态学研究的引物比较

为比较厌氧氨氧化细菌16SrRNA基因的2对特异性引物(Amx368F/Amx820R、Brod541F/Amx820R)在其分子生态学研究方面的优点与不足,本研究采用高通量测序和qPCR技术对长江口及其邻近海域沉积物中厌氧氨氧化细菌细菌的群落组成、多样性和丰度进行比较分析,结果表明,引物Amx368F/Amx820R特异性较高,能够较为完整地反映沉积物中厌氧氨氧化细菌的多样性信息,且其覆盖的厌氧氨氧化细菌丰度与功能基因hzo丰度正相关(P<0.01);而引物Brod541F/Amx820R特异性较低,覆盖部分厌氧氨氧化细菌及其他多个门的细菌,其量化的厌氧氨氧化细菌与功能基因hzo丰度无明显相关关系(P>0.05)。因此,引物Amx368F/Amx820R能够更加真实地反映厌氧氨氧化细菌在海洋沉积物中的群落特征,在其分子生态学研究中更具优势。     

Spatio-temporal variability of small fishes related with environmental factors in a typical domestic tap water lake,Eastern China

The knowledge of prey small ?sh stock, distribution and abundance is necessary to guide stocking of piscivorous ?sh for the biomanipulation in domestic tap water lakes. This study describes the current status of small ?sh community in Kuilei Lake(China), and examines the spatial and seasonal variations of the community in relation to key environmental factors. Based on submerged macrophyte cover and water depth, the lake was divided into ?ve major habitats:(1) macrophyte covered shallow habitat of water depth< 2.00 m,(2) uncovered or less-covered shallow habitat(2.00 m–3.50 m),(3) uncovered medium shallow habitat(3.50 m–5.00 m),(4) uncovered medium deep habitat(5.00 m–6.50 m) and(5) uncovered deep habitat(6.50 m–8.50 m). The abundance and composition of small ?sh were monitored by benthic fykenet sampling from April 2013 to January 2014. A total of 2881 individuals belonging to 5 families and 21 species were collected. Based on their abundance(accounted for 88.96% of the total) and occurrence(more than 33.33%), Acheilognathus chankaensis, Acheilognathus macropterus, Microphysogobio microstomus,Pseudorasbora parva and Rhinogobius giurinus were recognized as dominant small ?sh species. The results of correlation analysis identi?ed that species richness( Sr), Shannon-Wiener diversity index( H′)and Margalef′s richness index( D) were signi?cantly negatively correlated with water depth, but positively correlated with biomass of submerged macrophytes.Redundancy analysis(RDA) revealed that the spatial distributions of most small ?shes were negatively associated with water depth. The details of these ?ndings are bene?cial to understanding the adaptation of the small ?shes in degraded environments, and to developing suitable biomanipulation strategies for the management of ?sh resources and water quality in the lakes along the lower reach of the Changjiang(Yangtze) River basin.     

Archaeal community structure in sediments from a seamount in the Mariana Volcanic Arc

Seamounts are subsurface mountains in the ocean. Examination of the abundance and distribution of Archaea in seamount ecosystems may provide a better understanding of their ecological functions. Most studies of marine archaeal assemblages in seamount area have focused on hydrothermal vents or ferromanganese crusts. We investigated the archaeal communities from a seamount of the Mariana Volcanic Arc, in the tropical western Pacific Ocean by using high-throughput sequencing. Thaumarchaeota was dominant in the sediments of all sample stations. Community diversity and species richness were greatest at stations near the top of the seamount, and lowest at the deepest station. One sample station on the steep southeast slope that faced the Yap-Mariana trench had a unique composition of Archaea. In summary, depth has an important influence on archaeal community structure, and the geographic properties and sediment characteristics may explain the unique distribution patterns of Archaea in this seamount. This study provides a foundation for future research on Archaea in seamounts.     

寒武纪生物大爆发之后的底栖生态系统工程建造者:以河南地区为例

扰动生物作为生态系统工程建造者,通过对沉积底质的改造直接影响沉积物和水体之间的相互作用(营养物质的循环、迁移和存储),对底栖生态系统工程有重要的影响.遗迹化石作为底栖生物活动的载体,为研究寒武纪大爆发之后底栖生态系统工程建造者的演化规律,并评价底栖生态系统工程建造者对生态系统工程产生的影响提供了依据.对河南地区8个寒武...     

水下收缩裂隙形成过程及裂缝充填模式研究

通过野外实地考察测试,对水下收缩裂隙整体形成过程、裂隙内充填物沉积模式、影响因素等进行了详细讨论,并且建立了相应的地质理论模型。水下收缩裂隙的形成共分4个阶段,依次为:泥水混合物进入低洼地带的初始混浊状态;沉积压实稳定阶段;水位线下降,盐度增大,裂隙形成阶段;沉积裂隙充填阶段。其中“异常高压作用”,对于初始裂隙的形成、沉积物孔隙水的有效排出以及主要渗流通道的发育起到了重要作用。单一泥质地层裂隙内充填物沉积模式与沙泥互层的多旋回地层略有差异,主要表现为沙层的润滑作用以及对早期裂隙内充填物类型的影响。上述两种地层裂隙内充填物沉积特征均与后期充注期次相对应,表现出很好的韵律性。裂隙纵剖面中生物发育层的出现,对裂隙内充填物的物理、化学性质将产生重要影响。     

基于生态-经济重要性的滨湖城市土地开发适宜性分区研究——以无锡市为例

契爷石水库是广东省东莞市的一座中型供水水库,为富营养化水体.为改善该水库水质,2002年10月开始从东江调水,以满足供水的水量和水质要求.在调水后的两年期间,对水文、水质和浮游植物种类和数量进行了调查.本文将调查结果与以往(2000年)调查数据进行了对比,分析了调水后该水库水质和浮游植物的特点和调水对水质的影响.调水直接导致TP浓度显著下降,丰水期的TN浓度有一定下降,但调水并没显著降低枯水期TN的浓度.调水后,丰水期水体的叶绿素a浓度有明显的增加,枯水期叶绿素a浓度大大低于调水前.与调水前相比,调水后浮游植物种类数有明显增加,特别是绿藻种类,蓝藻中的假鱼腥藻和针状蓝纤维藻仍为浮游植物优势种,但硅藻中的短小曲壳藻和针杆藻等成为新的优势种,裸藻的数量大为减少.     

粘质沙雷氏菌(Serratia marcescens)发酵培养基优化的研究

采用单因素试验和正交试验,对从山东东营海岸湿地盐碱滩地土壤中筛选出的一株海洋菌种———粘质沙雷氏菌(Serratia marcescens)的发酵培养基进行优化,并进行100 L发酵罐中试放大试验的研究。确定粘质沙雷氏菌的最佳培养基配方为葡萄糖10 g/L,硫酸铵5 g/L,麸皮50 g/L,柠檬酸三钠1.0 g/L,K2HPO4.3H2O 0.3 g/L,FeSO4.7H2O 0.05 g/L,MgSO4.7H2O 0.5 g/L,pH 7.2~7.7。发酵最适温度为30℃。通过测定粘质沙雷氏菌在发酵罐中培养的生长曲线,确定发酵时间以28~30 h为宜,发酵结束后发酵液中的活菌数约为50×108个/mL。将所筛选到的粘质沙雷氏菌应用于农作物的病害防治,效果非常显著,表明是一株高活性的生物防治拮抗菌。此研究结果为高效率、低成本和工业化生产具有生物防治作用的海洋菌种制剂提供了科学依据,也为海岸湿地盐碱土的可持续开发利用提供了技术支撑。     

闽三角红树林底栖软体动物共存格局及机制

以漳江口国家级红树林自然保护区、九龙江口省级红树林自然保护区和泉州湾省级湿地保护区3个红树林分布区作为研究对象,研究底栖软体动物在中等尺度下的共存格局及机制。利用非度量多维标度分析、基于蒙特卡洛的零模型和网络分析探讨底栖软体动物的共存格局,利用raup-crik零模型识别决定性过程和随机过程测定底栖软体动物的相对重要性。结果显示,漳江口、九龙江口和泉州湾湿地共发现37种底栖软体动物,底栖软体动物的平均密度、物种数及群落物种组成差异显著。软体动物的共存格局呈现非随机分散和模块化,这些格局同时受到决定性过程和随机过程的调控作用,其中,物种竞争起主导作用。