细胞色素b基因序列与7种石首鱼类的系统进化

通过比较一段381bp细胞色素b基因序列，分析了石首鱼科7种石首鱼的系统发育关系．该序列有51个单变异多态位点、128个简约信息多态位点，对应的氨基酸序列有41个氨基酸变异位点．根据Kimura 2参数构建的邻接树(NJ tree)和最大简约树(MP tree)都显示同样的结果：7种石首鱼类构成一个单系群．皮氏叫姑鱼位于单系群的基部，且与其他石首鱼分离时间很早；大黄鱼和棘头梅童鱼亲缘关系最近。并与钩牙皇石首鱼和波纹短须石首鱼构成姐妹群．黄姑鱼和鱿鱼也接近树的基部．     

Surface geostrophic currents across the Antarctic circumpolar current in Drake Passage from 1992 to 2004

The Southern Ocean plays an important role in the global overturning circulation as a significant proportion of deep water is converted into intermediate and deeper water masses in this region. Recently, a secular trend has been reported in wind stress around the Southern Ocean and it is thought theoretically that the strength of the ACC is closely related to wind stress, so one consequence should be a corresponding increase in ACC transport and hence changes in the rate of the global overturning. There are no long-term data sets of ACC transport and so we must examine other data that may also respond to changing wind stress. Here we calculate surface currents in Drake Passage every seven days over 11.25 years from 1992 to 2004. We combine surface velocity anomalies calculated from satellite altimeter sea surface heights with measured surface currents. Since 1992, the UK has regularly occupied WOCE hydrographic section SR1b across the ACC in Drake Passage. From seven hydrographic sections surface currents are estimated by referencing relative geostrophic velocities from CTD sections with current measurements made by shipboard and lowered acoustic Doppler current profilers. Combining the seven estimates of surface currents with the altimeter data reduces bias in the estimates of average currents over time through Drake Passage and we show that surface current anomalies estimated by satellite and in situ observations are in good agreement. The strongest surface currents are found in the Subantarctic and Polar Fronts with average speeds of 50 cm/s and 35 cm/s, respectively and are inversely correlated, so that maximum westward flow in one corresponds to minimum westward flow in the other. The average cross-sectional weighted surface velocity from 1992 to 2004 is 16.7 ± 0.2 cm/s. A spectral analysis of the average surface current has only weakly increasing energy at higher frequencies and there is no dominant mode of variability. The standard deviation of the seven day currents is 0.68 cm/s and a running 12 month average has only a slightly smaller standard deviation of 0.52 ± 0.16 cm/s. The southern annular mode (SAM) measures the circumpolar average of wind stress and like the surface currents its spectrum has slightly increased energy at frequencies greater than 1 cpy. A cospectral analysis of these, averaging cospectra of five slightly overlapping 36 month segments improve statistical reliability, suggests that there is coherence between them at 1 cpy with the currents leading changes in the Southern annular mode. We conclude that the SAM and average Drake Passage surface currents are weakly correlated with no dominant co-varying modes, and hence predicting Southern Ocean transport variability from the SAM is not likely to give significant results and that secular trends in surface currents are likely to be masked by weekly and interannual variability.     

Form Drag Caused by Topographically Forced Waves in a Barotropic β Channel: Effect of Higher Mode Resonance

The relationship between form drag and the zonal mean velocity of steady states is investigated in a very simple system; a barotropic quasi-geostrophic b2; channel with sinusoidal topography. When a steady solution is calculated by the modified Marquardt method, keeping the zonal mean velocity constant as a parameter, the characteristic of the solution changes at a phase speed of a wave with a wavenumber higher than that of the bottom topography. For velocities smaller than this critical value, there exists a stable quasi-linear solution similar to the linear solution. For larger velocities, there exist three solutions whose form drag is very large. In addition, the resonant velocity of the mode, whose wavenumber is the same as the bottom topography, has no effect on these solutions. When the quiescent fluid is accelerated by a constant wind stress, acceleration stops around the critical velocity for a wide range of the wind stress. If the wind stress is too large for the acceleration to stop, the zonal current speed continues to increase infinitely. It is implied that the zonal velocity of equilibrium is mainly determined, not by the wind stress, but by the amplitude of the bottom topography and the viscosity coefficient.     

6种笛鲷属鱼类Cyt b基因片段序列的比较

采用聚合酶链式反应直接测序法，获得紫红笛鲷(Lutjanus argentimaculatus)、白星笛鲷(L．stellatus)、千年笛鲷(L．sebae)、勒氏笛鲷(L．russelli)、红鳍笛鲷(L．erythropterus)5种笛鲷属鱼的线粒体细胞色素b(Cyt b)425bp序列，并结合基因库中的斜带笛鲷(L．decussatus)Cytb同源序列进行比较分析，共发现88个核苷酸位点存在变异(20．7％)。用MEGA2．1软件中的Pairwise distance工具计算了6种笛鲷属鱼类的相对遗传距离，表明6种笛鲷属鱼类的序列差异在0．096-0．129之间，其中红鳍笛鲷和紫红笛鲷序列差异最大为0．129，千年笛鲷与红鳍笛鲷的序列差异最小为0．096；序列变异的转换／颠换比值较低，平均只有2．160。     

Mid–late Holocene monsoon climate retrieved from seasonal Sr/Ca and δO records of Porites lutea corals at Leizhou Peninsula, northern coast of South China Sea

South China Sea (SCS) is a major moisture source region, providing summer monsoon rainfall throughout Mainland China, which accounts for more than 80% total precipitation in the region. We report seasonal to monthly resolution Sr/Ca and b4;¹⁸O data for five Holocene and one modern Porites corals, each covering a growth history of 9–13 years. The results reveal a general decreasing trend in sea surface temperature (SST) in the SCS from 6800 to 1500 years ago, despite shorter climatic cycles. Compared with the mean Sr/Ca–SST in the 1990s (24.8 °C), 10-year mean Sr/Ca–SSTs were 0.9–0.5 °C higher between 6.8 and 5.0 thousand years before present (ky BP), dropped to the present level by 2.5 ky BP, and reached a low of 22.6 °C (2.2 °C lower) by 1.5 ky BP. The summer Sr/Ca–SST maxima, which are more reliable due to faster summer-time growth rates and higher sampling resolution, follow the same trend, i.e. being 1–2 °C higher between 6.8 and 5.0 ky BP, dropping to the present level by 2.5 ky BP, and reaching a low of 28.7 °C (0.7 °C lower) by 1.5 ky BP. Such a decline in SST is accompanied by a similar decrease in the amount of monsoon moisture transported out of South China Sea, resulting in a general decrease in the seawater b4;¹⁸O values, reflected by offsets of mean b4;¹⁸O relative to that in the 1990s. This observation is consistent with general weakening of the East Asian summer monsoon since early Holocene, in response to a continuous decline in solar radiation, which was also found in pollen, lake-level and loess/paleosol records throughout Mainland China. The climatic conditions 2.5 and 1.5 ky ago were also recorded in Chinese history. In contrast with the general cooling trend of the monsoon climate in East Asia, SST increased dramatically in recent time, with that in the 1990s being 2.2 °C warmer than that 1.5 ky ago. This clearly indicates that the increase in the concentration of anthropogenic greenhouse gases played a dominant role in recent global warming, which reversed the natural climatic trend in East Asian monsoon regime.     

Factors controlling spatial and seasonal distributions of precipitation δ18O in China

Utilising datasets from the Global Network of Isotopes in Precipitation of the International Atomic Energy Agency, and previous isotopic studies, we investigated δ¹⁸O spatial and temporal patterns in Chinese precipitation. Significantly positive relationships existed between precipitation δ¹⁸O and air temperature above the north of 35°N and in high altitude regions above 32°N. Significantly negative relationships between precipitation δ¹⁸O and the precipitation amount existed below south of 35°N. These temperature and precipitation effects became stronger with increasing altitude except in high altitude regions between 32°N and 35°N. The NCEP/NCAR reanalysis data from 1980 to 2004 showed that variations in spatial and seasonal wind fields at 700 hpa and total precipitable water from the ground to the top of the atmosphere were correlated with the monthly spatial distribution of precipitation δ¹⁸O. Basing on this relationship, we established quantitative correlations between the mean monthly precipitation δ¹⁸O and both latitude and temperature in different seasons. We found that spatial variations in precipitation δ¹⁸O could be described well using the Bowen–Wilkinson model and second‐order equations developed during the present study only in winter (from December to February). During the rest of the year, patterns were too complex to predict using simple models. The results suggest that it is difficult to demonstrate variations of precipitation δ¹⁸O throughout the year and for all regions of China using a single model. Moreover, the new models for the relationships among precipitation, latitude, and temperature were better able to depict the variations in precipitation δ¹⁸O than the Bowen–Wilkinson model. Copyright © 2011 John Wiley & Sons, Ltd.     

粤西河台金矿田糜棱岩XRD特征及其地质意义

粤西河台金矿田位于钦州湾—杭州湾（钦—杭）结合带南段的云开地区,是典型的受韧性剪切带控制的金矿。然而,对于剪切带中有利于金成矿的地质环境及其成矿过程仍然存在争议。本文在对河台矿区高村矿床进行详细野外地质调查的基础上,通过X射线衍射（XRD）对含金剪切带中各类糜棱岩系列岩石进行系统研究,建立应力变化—成分变异的对比序列,从而进一步揭示剪切变形与金矿化的关系。结果显示,剪切带中白（绢）云母的含量在9%~40%,并且在初糜棱岩、无矿糜棱岩、含矿糜棱岩、无矿超糜棱岩、含矿超糜棱岩中呈现出逐渐递增的趋势。白（绢）云母主要为2M1型,b0值在 0.55505~0.99002 nm之间,初糜棱岩＜无矿糜棱岩＜ 无矿超糜棱岩,分别指示低压、中压和高压环境。可见,从初糜棱岩到超糜棱岩,随着变形强度的增加,热液活动也随之增强,从而新生成了大量白（绢）云母。然而,在含矿的糜棱岩和超糜棱岩中还存在一些低压白云母,推测其可能是在后期脆性裂隙中由于流体活动所新形成的。因此,成矿可能经历了两个阶段,即早期的韧性剪切变形阶段导致金的初步富集,后期叠加的脆性破裂阶段形成具有工业品位的矿体。另外,无矿超糜棱岩的石英含量（29.8%）明显低于糜棱岩（44.2%）,推测在形成石英脉型矿体过程中,部分热液可能来自于其围岩超糜棱岩。因此,相对于初糜棱岩和糜棱岩,超糜棱岩中更有利于金矿的形成。     

Ongoing methane discharge at well site 22/4b (North Sea) and discovery of a spiral vortex bubble plume motion

First direct evidence for ongoing gas seepage activity on the abandoned well site 22/4b (Northern North Sea, 57°55′ N, 01°38′ E) and discovery of neighboring seepage activity is provided from observations since 2005. A manned submersible dive in 2006 discovered several extraordinary intense seepage sites within a 60 m wide and 20 m deep crater cut into the flat 96 m deep seafloor. Capture and (isotope) chemical analyses of the gas bubbles near the seafloor revealed in situ concentrations of methane between 88 and 90%Vol. with δ¹³C–CHb>4b> values around −74‰ VPDB, indicating a biogenic origin. Bulk methane concentrations throughout the water column were assessed by 120 Niskin water samples showing up to 400.000 nM CHb>4b> in the crater at depth. In contrast, concentrations above the thermocline were orders of magnitude lower, with a median value of 20 nM. A dye tracer injection into the gas seeps revealed upwelling bubble and water motion with gas plume rise velocities up to ∼1 ms⁻¹ (determined near the seabed). However, the dissolved dye did not pass the thermocline, but returned down to the seabed. Measurements of direct bubble-mediated atmospheric flux revealed low values of 0.7 ± 0.3 kty⁻¹, much less than current state-of-the-art bubble dissolution models would predict for such a strong and upwelling in situ gas bubble flux at shallow water depths (i.e. ∼100 m).Acoustic multibeam water column imaging data indicate a pronounced 200 m lateral intrusion at the thermocline together with high methane concentration at this layer. A partly downward-orientated bubble plume motion is also visible in the acoustic data with potential short-circuiting in accordance to the dye experiment. This observation could partly explain the observed trapping of most of the released gas below the well-established thermocline in the North Sea. Moreover, 3D analyses of the multibeam water column data reveal that the upwelling plume transforms into a spiral expanding vortex while rising through the water column. Such a spiral vortex motion has never been reported before for marine gas seepage and might represent an important process with strong implication on plume dynamics, dissolution behavior, gas escape to the atmosphere, and is considered very important for respective modeling approaches.     

Genetic structure of the reef grouper Epinephelus merra in the West Indian Ocean appears congruent with biogeographic and oceanographic boundaries

The reef fauna connectivity of the West Indian Ocean (WIO) is one of the least studied globally.&nbsp;Here we use genetic analyses of the grouper Epinephelus merra (Bloch 1793) to determine patterns of connectivity and to identify barriers to dispersal in this WIO marine area. Phylogeographic and population‐level analyses were conducted on cytochrome b sequences and microsatellites (13 loci) from 557 individuals sampled in 15 localities distributed across the West Indian Ocean. Additional samples from the Pacific Ocean were used to benchmark the WIO population structure. The high level of divergence revealed between Indian and Pacific localities (of about 4.5% in sequences) might be the signature of the major tectonic and climatic changes operating at the Plio‐Pleistocene transition, congruently with numerous examples of Indo‐Pacific speciation. In comparison, the E.&nbsp;merra sequences from the Indian Ocean constitute a monophyletic clade with a low average genetic distance (d&nbsp;<&nbsp;0.5%). However both genetic markers indicated some structure within this ocean. The main structure revealed was the isolation of the Maldives from the WIO localities (a different group signature identified by clustering analysis, great values of differentiation). Both marker types reveal further significant structure within the WIO, mainly the isolation of the Mascarene Islands (significant AMOVA and isolation‐by‐distance patterns) and some patchy structure between the northernmost localities and within the Mozambique Channel. The WIO genetic structure of E.&nbsp;merra appeared congruent with main biogeographic boundaries and oceanographic currents.     

PEPSI: The high‐resolution échelle spectrograph and polarimeter for the Large Binocular Telescope

PEPSI is the bench‐mounted, two‐arm, fibre‐fed and stabilized Potsdam Echelle Polarimetric and Spectroscopic Instrument for the 2×8.4 m Large Binocular Telescope (LBT). Three spectral resolutions of either 43 000, 120 000 or 270 000 can cover the entire optical/red wavelength range from 383 to 907 nm in three exposures. Two 10.3k×10.3k CCDs with 9‐µm pixels and peak quantum efficiencies of 94–96 % record a total of 92 échelle orders. We introduce a new variant of a wave‐guide image slicer with 3, 5, and 7 slices and peak efficiencies between 92–96 %. A total of six cross dispersers cover the six wavelength settings of the spectrograph, two of them always simultaneously. These are made of a VPH‐grating sandwiched by two prisms. The peak efficiency of the system, including the telescope, is 15 % at 650 nm, and still 11 % and 10 % at 390 nm and 900 nm, respectively. In combination with the 110 m² light‐collecting capability of the LBT, we expect a limiting magnitude of ≈20th mag in V in the low‐resolution mode. The R = 120 000 mode can also be used with two, dual‐beam Stokes IQUV polarimeters. The 270 000‐mode is made possible with the 7‐slice image slicer and a 100‐µm fibre through a projected sky aperture of 0.74″, comparable to the median seeing of the LBT site. The 43 000‐mode with 12‐pixel sampling per resolution element is our bad seeing or faint‐object mode. Any of the three resolution modes can either be used with sky fibers for simultaneous sky exposures or with light from a stabilized Fabry‐Pérot étalon for ultra‐precise radial velocities. CCD‐image processing is performed with the dedicated data‐reduction and analysis package PEPSI‐S4S. Its full error propagation through all image‐processing steps allows an adaptive selection of parameters by using statistical inferences and robust estimators. A solar feed makes use of PEPSI during day time and a 500‐m feed from the 1.8 m VATT can be used when the LBT is busy otherwise. In this paper, we present the basic instrument design, its realization, and its characteristics. Some pre‐commissioning first‐light spectra shall demonstrate the basic functionality. (© 2015 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)