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141.
142.
大口黑鲈抗菌肽hepcidin cDNA序列和结构分析 总被引:2,自引:0,他引:2
以大口黑鲈为材料,提取肝脏总RNA,经RT-PCR扩增出hepcidin cDNA的开放阅读框(ORF)及3′端非编码区序列,应用5′RACE方法得到大口黑鲈hepcidin cDNA5′末端。将所获得的两个片段分别克隆到T载体后进行测序,并拼接成大口黑鲈hepcidin全长cDNA。序列分析表明:大口黑鲈hepcidin全长cDNA为564bp,含有一个258bp的ORF,编码86个氨基酸残基,由信号肽(24个残基)、前肽(42个残基)和成熟肽(20个残基)3部分组成hepcidin前体。在前肽部分具有前肽转化酶典型的RX(K/R)R基元,成熟肽部分含有8个保守的半胱氨酸残基,可形成四个链内二硫桥,使β-折叠结构保持稳定。大口黑鲈Hepcidin与其他鱼类的同源性在29.7%~90.5%间,尤其是信号肽区域,与鳜、尼罗罗非鱼、真鲷、花鲈、黑鯛、金眼狼鲈仅有2~3个氨基酸的差别。 相似文献
143.
综合运用高精度层序地层学、构造一地层学方法,认为十屋断陷深层发育的各类隐蔽圈闭是下一步勘探工作的重要目标。同时,由于构造对沉积的控制作用,在断陷内部不同的构造部位具有不同的隐蔽圈闭分布特征。十屋断陷西部皮家地区、北部张家屯一八屋地区位于油气主要运移指向区,分别由断裂坡折带和以挠曲型褶皱为特征的弯折带控制的储集砂体十分发育,盖层及保存条件也十分优越,是深部原生岩性、地层油气藏最有利的勘探目标区带。 相似文献
144.
We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commer-cial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The re-suits showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient's fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×108, 1.25×108 and 4.7×101 respectively. The detecting limit of NLVs was 1×101 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future. 相似文献
145.
146.
鄂尔多斯盆地石炭系厚 0~ 6 0 0m ,属潮坪三角洲相。从鄂尔多斯沉积特征出发 ,由地表露头结合井下剖面讨论了该盆地石炭系层序地层特点。识别出两类层序界面。依层序界面特征划分出两个层序 ,分属于本溪期及太原期 ,每个层序由海进体系域和高水位体系域组成 ,缺失低水位体系域。讨论了凝缩段及体系域的分布。 相似文献
147.
鄂尔多斯盆地前寒武纪末期古地貌恢复及其对烃源岩的控制作用 总被引:1,自引:0,他引:1
鄂尔多斯盆地前寒武纪末期古地貌控制着后期沉积体系的空间配置与古地理展布。通过野外剖面、岩芯分析、测井数据等建立鄂尔多斯盆地寒武系层序地层格架;选取寒武纪SS1最大海泛面(徐庄组沉积晚期地层)为标志层,通过残余厚度印模法,并结合古地质图分析,对研究区前寒武纪进行古地貌恢复,明确了前寒武纪沉积背景、构造格局及物源方向。总体上,前寒武纪鄂尔多斯盆地南、北濒临秦祁海和兴蒙海,东、西被贺兰和晋豫陕坳拉槽所夹持,古地貌东北高、西南低,受秦祁海影响最大,可将其划分为3个古构造地貌单元:高地剥蚀区、受古构造控制形成的坳陷区以及处于剥蚀与沉积过渡地带的斜坡阶地区。通过研究前寒武纪末期古地貌与寒武纪早期烃源岩分布认为,拉张活动背景下的坳陷带以及斜坡带是鄂尔多斯盆地寒武纪早期烃源岩发育的有利区块,前寒武纪古地貌格局影响了寒武纪早期烃源岩发育与分布。恢复古地貌形态对于揭示物源供给,研究沉积物搬运、平面和纵向展布特征,探究沉积、沉降中心的迁移规律,以及进一步揭示生储盖组合和分布具有重大意义。 相似文献
148.
胶莱盆地东缘早白垩世早期莱阳群沉积体现了湖盆由开始形成—鼎盛—萎缩消亡的全过程,发育由冲洪积相—湖相—三角洲相—河流相沉积的完整序列,且由东至西,由湖相到河流相逐渐过渡;古水流方向显示莱阳期盆地中心位于朱吴断裂、海阳断裂带之间,以断裂带为界,以西水流方向大致为由NW至SE向,以东水流方向则为由SE至NW向;早白垩世早期莱阳期盆地的形成与演化明显受区域性牟(平)-即(墨)断裂带活动所控制。 相似文献
149.
Miquel Poyatos‐Mor George D. Jones Rufus L. Brunt Daniel E. Tek David M. Hodgson Stephen S. Flint 《Basin Research》2019,31(5):920-947
Exhumed basin margin‐scale clinothems provide important archives for understanding process interactions and reconstructing the physiography of sedimentary basins. However, studies of coeval shelf through slope to basin‐floor deposits are rarely documented, mainly due to outcrop or subsurface dataset limitations. Unit G from the Laingsburg depocentre (Karoo Basin, South Africa) is a rare example of a complete basin margin scale clinothem (>60 km long, 200 m‐high), with >10 km of depositional strike control, which allows a quasi‐3D study of a preserved shelf‐slope‐basin floor transition over a ca. 1,200 km2 area. Sand‐prone, wave‐influenced topset deposits close to the shelf‐edge rollover zone can be physically mapped down dip for ca. 10 km as they thicken and transition into heterolithic foreset/slope deposits. These deposits progressively fine and thin over tens of km farther down dip into sand‐starved bottomset/basin‐floor deposits. Only a few km along strike, the coeval foreset/slope deposits are bypass‐dominated with incisional features interpreted as minor slope conduits/gullies. The margin here is steeper, more channelized and records a stepped profile with evidence of sand‐filled intraslope topography, a preserved base‐of‐slope transition zone and sand‐rich bottomset/basin‐floor deposits. Unit G is interpreted as part of a composite depositional sequence that records a change in basin margin style from an underlying incised slope with large sand‐rich basin‐floor fans to an overlying accretion‐dominated shelf with limited sand supply to the slope and basin floor. The change in margin style is accompanied with decreased clinoform height/slope and increased shelf width. This is interpreted to reflect a transition in subsidence style from regional sag, driven by dynamic topography/inherited basement configuration, to early foreland basin flexural loading. Results of this study caution against reconstructing basin margin successions from partial datasets without accounting for temporal and spatial physiographic changes, with potential implications on predictive basin evolution models. 相似文献
150.
The northeastern shelf margin of the South China Sea(SCS) is characterized by the development of large scale foresets complexes since Quaternary. Based on integral analysis of the seismic, well logging and paleontological data, successions since ~3.0 Ma can be defined as one composite sequence, consist of a set of regional transgressive to regressive sequences. They can be further divided into six 3 rd order sequences(SQ0–SQ5) based on the Exxon sequence stratigraphic model. Since ~1.6 Ma, five sets of deltaic systems characterized by development of wedge-shaped foresets complexes or clinoforms had been identified. High-resolution seismic data and the thick foresets allowed further divided of sub-depositional sequences(4 th order) of regression to transgression, which is basically consistent with published stacked benthic foram O-isotope records. Depositional systems identified in the study area include deltaic deposits(inner-shelf deltas and shelf-edge deltas), incised valleys, and slope slumping massive deposits. Since ~1.6 Ma, clinoforms prograded from the southern Panyu Lower Uplift toward the northern Baiyun Depression, shelf slope break migrated seaward, whereas the shelf edge of SQ0 migrated landward. The development of incised valleys in the continental shelf increased upward,especially intensive on the SB3 and SB2. The slumping massive deposits increased abruptly since SB2, which corresponds to the development of incised valleys. The evolution of depositional systems of continental slope mainly controlled by the combined influence of sea level changes, tectonic movements, sediment supply and climate changes. Since ~3.0 Ma, relative sea level of the northern SCS had been experienced transgression(~3.0 Ma BP) to regression(~1.6 Ma BP). The regional regression and maximum transgressions of the composite sequences were apparently enhanced by uplift or subsidence related to tectono-thermal events. In addition,climatic variations including monsoon intensification and the mid-Pleistocene transition may have enhanced sediment supply by increasing erosion rate and have an indispensable influence on the development of the incised valleys and 5 sets of deltaic systems since ~1.6 Ma. 相似文献