Crystallographic and NMR studies on sterols from green alga Chaetomorpha basiretorsa Setchell

Chemical investigation of the ethanol extract of the marine green alga Chaetomorpha basiretorsa Setchell led to the isolation of a new sterol stigmast-4,28-dien-3α,6β-diol 1 in addition to the five known sterols of β-lawsaritol 2, saringosterol 3, 24-hydroperoxy-24-vinyl - cholesterol 4, β-stigmasterol 5, 29-hydroxystigmasta-5, 24 （28） -dien-3β-ol 6. Compounds were isolated by normal phase silica gel and Sephadex LH - 20 gel colum chromatography, reverse phase HPLC and recrystalization. Their structures were elucidated by spectroscopic methods including MS, IR 1D/2D NMR and X-ray analysis. Cytotoxicity of compounds was screened by using the standard MTF method. All these compounds were isolated from the green alga Chaetomorpha basiretorsa Setchell for the first time and they were inactive （50% inhibitory concentration was greater than 10 μg/cm^3） against KB, Bel -7402, PC - 3M, Ketr 3 and MCF-7 cell lines.     

Analysis on the indicator species and ecological groups of pelagic ostracods in the East China Sea

Ecological adaptation and ecological groups of pelagic ostracods were examined in the East China Sea （23°30′-33°00′N, 118°30′ -128°00′E）, in relation to temperature and salinity. The data were collected in four surveys conducted from 1997 to 2000. The density, yield density, or negative exponent models were used to determine the optimal temperature and salinity of water for the thriving growth of pelagic ostracods. Thereafter, ecological groups and potential distribution patterns of pelagic ostracods were determined based on the predicted parameters such as optimal temperature and salinity, consulting the geographic distribution. The analytical results indicate that, among the numerical dominant pelagic ostracods in the East China Sea （ECS）, Euconchoecia aculeata, E. elongata, E. chierchiae, E. maimai, and Cypridina dentata, etc. are offshore subtropical water species. These species are widely distributed in the area, and they can be brought by the warm current to north offshore during spring and winter. The predicated optimal temperature （OT） and optimal salinity （OS） for Paraconchoecia decipiens, P. echinata, P. spini- fera, P. oblonga, Conchoecia magna and Porroeciaporrecta are all greater than 25℃ and 34 separately. These species are mainly distributed in the waters of the Kuroshio, the Taiwan Warm Current, and the Taiwan Strait, and therefore are designated as ocean- ic tropical water species. On the other hand, Pseudoconchoecia concerttrica is considered as offshore subtropical water species based on its geographical distribution although its OT is 19℃. The other species, though their OSs are approximately 34 and with OTs ranging from 20° to 25℃, are considered as offshore subtropical water species because they were found to be widely distributed from the South China Sea to the East China Sea.     

应用能量色散X荧光衍射仪分析海绵元素组成

通过能量色散X荧光衍射仪（EDXRF）对3种南海潮间带海绵：细薄星芒海绵Stelletta tenui（Lindgren）、澳大利亚厚皮海绵Craniella australiensis （Carter）和多皱软海绵Halichondria hugosa （Ridley＆Dendy）的元素组成和含量进行了研究,并与两种黄海潮间带海绵进行了比较。结果表明,海绵体内的元素组成受种属差异的影响较大,溴元素的含量可能同一定的活性物质产生相关。另外,不同海绵具有骨针形态的多样性,为深入研究内部合成机制提供了材料。     

城市地理研究中的单分形、多分形和自仿射分形

分形几何学在城市地理研究中具有广泛的应用,然而很多基本概念却让初学者感到迷惑。如何区分单分形、自仿射分形与多分形,是一个基本而重要的问题。简单分形容易理解,而真实的地理现象很少是单分形的。城市生长过程具有自仿射特征,而城市空间格局却具有多分形性质。作者发现,各种分形的共性在于三个方面：标度律、分数维和熵守恒。论文基于标度、分维和熵守恒公式,借助隐喻城市生长的规则分形来区分单分形、多分形和自仿射分形,讨论分形系统演化的机理、分形与空间自相关和空间异质性的联系,同时澄清一些在地理分形研究中的常见错误概念。最后以城市位序-规模分布为例,说明并对比单分形和多分形在城市地理研究中的建模与应用思路。     

基于转录组测序对两种氮素营养条件下多形微眼藻氮代谢途径的解析

探究微藻的氮代谢通路对了解其对不同氮源利用的分子机制具有重要意义。本研究利用Illumina高通量测序技术对两种氮素营养条件下(硝酸氮和尿素)多形微眼藻的转录组进行分析,通过基因功能注释及数字基因表达谱分析,研究了多形微眼藻细胞内氮代谢的调控机制。结果检测出15种参与氮代谢的酶,对应76个编码基因,构建了多形微眼藻的氮代谢通路图。其中10个酶编码基因在两种不同氮素营养条件下存在差异表达,最显著的是谷氨酸合成酶、谷氨酸脱氢酶和谷氨酰胺合成酶相关基因。有机氮源(尿素)实验组中,多形微眼藻细胞内的硝酸盐还原酶、亚硝酸盐还原酶等基因的差异表达明显高于无机氮源(硝酸钠)实验组,表明当环境中的氮源为尿素时,会对多形微眼藻细胞内硝酸盐的转化和利用有一定影响。本研究初步阐述了硝酸盐、尿素的吸收转运对多形微眼藻细胞内氮代谢的影响机制,可为硅藻在不同氮素营养条件下的吸收利用机制及氮代谢响应研究提供依据。     

降低受信仪行程开关返程误差方法的分析

由于受信仪的行程开关返程误差较大,导致了运行、射界范围的控制精度不高。 针对返程误差偏大实际问题,研究了受信仪的行程开关工作原理,建立了其工作过程数学模型,采用复合形法开展了参数优化设计。 依据 PDCA 程序,应用因果图分析方法获得影响误差的主要因素,并逐一采取相应的解决措施。 经过验证,其实现了凸轮行程开关的返程误差减小到 0. 5°以下,提高了发射装置运行、射界范围的控制精度,达到了预期的目标。     

基于AI的胸部CT平扫对冠脉钙化积分的准确性评价

目的:探讨基于人工智能(AI)的非门控胸部低剂量CT(LDCT)平扫与心电门控CT血管造影(ECG gated-CTA)对冠状动脉钙化积分(CACS)危险度分层的一致性.方法:回顾性分析行冠状动脉CTA扫描的100例患者,所有患者均行冠脉ECG gated-CTA扫描和常规非门控胸部LDCT平扫.在Siemens后处理...     

CT淋巴管成像对原发性乳糜尿患者诊断及分级的应用价值初探

目的：探讨CT淋巴管成像（CTL）对于原发性乳糜尿患者诊断及分级的应用价值。方法：回顾性收集确诊为原发性乳糜尿的79例患者的临床及CTL影像学资料,CTL指标主要包括：(1)肾脏受累侧别及肾内淋巴管分布范围：单肾、双肾、肾窦、肾实质及肾门上区、肾门下区;(2)腹膜后异常淋巴管分布情况：包括腹膜后、腰干区域、肾血管周、肾周脂肪囊、肾上腺区域等;(3)腹盆部及胸部扩张淋巴管分布及淋巴反流情况;(4)其他异常表现：包括腹盆部、胸部及骨骼等部位的淋巴管相关异常改变;(5)影像学分级：按照腹膜后累及区域范围进行影像学分级,将腹膜后区域分为左侧和右侧肾门上区、肾门下区、腰干区、肾血管旁区、肾周区等10个区域,累及5个及以内区域为轻度,累及6个及以上区域为重度。根据乳糜尿临床分级标准进行临床分级,对于原发性乳糜尿患者临床特点及CTL征象采用分类变量资料中的构成比进行统计描述,利用一致性Kappa检验评估原发性乳糜尿患者影像分级及临床分级的一致性。结果：79例CTL示肾内异常碘油沉积74例（93.7%）,单肾55例（69.6%）,双肾19例（24.1%）,肾窦74例（93.7%）,肾实质37例（46....     

采动裂隙“O形圈”时空演化规律及注浆应用

采动覆岩离层注浆充填技术对工作面隔离煤柱的尺寸有严格要求,已完成布置的工作面实施离层注浆时改变煤柱宽度较为困难,因此会对该技术的实施造成一定影响。基于此,对采动裂隙“O形圈”时空演化规律开展了理论分析和相似模拟研究,提出了采动裂隙“O形圈”注浆技术,并在夏店煤矿注浆工作面成功进行应用。注浆结束后的地表岩移成果表明,隔离煤岩柱整体支撑能力满足离层注浆承载要求,3119工作面注浆总量未发生异常,“O形圈”注浆对煤柱加固作用明显,3117面地表并未发生异常沉降。