Assessing neuroprotective P-glycoprotein activity at the blood-brain barrier in killifish (Fundulus heteroclitus) using behavioural profiles

The neuroprotective role of P-glycoprotein, a multixenobiotic resistance transporter (ABCB1/MDR1), in the blood-brain barrier in fish was examined using behavioural toxicological assays. P-glycoprotein acts as cellular efflux pump to prevent substrates from accumulating in the brain, including environmental contaminants such as ivermectin, a common aquaculture pesticide and mammalian anti-parasitic drug. The behavioural toxicological assays were developed to determine the neuropathological effect of ivermectin in killifish (Fundulus heteroclitus). P-glycoprotein function and thus blood-brain barrier integrity can be compromised by chemosensitizers that inhibit transport activity. Fish treated with ivermectin and the P-glycoprotein inhibitor cyclosporin A were significantly more sensitive and succumbed more rapidly to tilting, lethargy, slowing of pectoral-fin movement and loss of haptic-reactivity compared to fish treated with ivermectin-only. P-glycoprotein inhibition is associated with significantly earlier onset and increased mortality in ivermectin-exposed fish. Our results suggest that P-glycoprotein confers resistance against ivermectin-induced behavioural neuropathology and mortality in fish. This assay provides us with a non-invasive tool to study P-glycoprotein function in the blood-brain barrier and evaluate the behavioural effects of potential environmental neurotoxins.     

Model computations of Schumann resonance on Titan

We model the global electromagnetic (Schumann) resonance in the atmosphere of Titan. Parameters of conductivity of the lower ionosphere were implemented taken from existing aeronomic models of Titan's atmosphere. Two exponential conductivity profiles were constructed: one of them suggests favorable conditions for Schumann resonance and the other models considerable attenuation in the ionospheric plasma. Peak frequencies and Q-factors of resonance were computed as well as resonance spectra for the signals arriving from individual vertical lightning discharges and from strokes uniformly distributed over the planet. The models show that detection of Schumann resonance on Titan is feasible, especially in favorable conditions. Possible applications of Schumann resonance in the studies of Titan's lightning activity are outlined.     

Sea‐level response at Nain,Labrador, to atmospheric pressure and wind

Abstract

Two years of subtidal sea‐level data from Nain, Labrador, are analysed in terms of local atmospheric pressure and the two components of geostrophic wind or stress. Frequency‐dependent response coefficients are determined by multiple regression analysis involving inversion of the cross‐spectral matrix of the inputs. At very low frequencies the response to pressure is isostatic and the wind stress coefficients are consistent with those determined by Thompson et al. (1985) from analysis of a longer series of monthly means. There is very little change in the response between icy and ice‐free seasons. The wind, or stress, coefficients correspond to geostrophic set‐up by a narrow longshore current but do not show as much of an increase of phase lag with increasing frequency as expected. The pressure response is less than isostatic and lags as the frequency increases from zero to about 0.02 cph. Possible reasons for this are discussed. Removal of wind as well as pressure effects ffom the sea‐level data makes only minor changes to the monthly mean residual sea‐level.     

The influence of the Madden-Julian oscillation on high-latitude surface air temperature during boreal winter

By analyzing NCEP-NCAR reanalysis daily data for 1979–2016, the modulation by Madden-Julian Oscillation (MJO) of the wintertime surface air temperature (SAT) over high latitude is examined. The real-time multivariate MJO (RMM) index, which divides the MJO into eight phases, is used. It is found that a significantly negative SAT anomaly over the northern high latitude region of (180°–60 °W, 60°–90 °N) lags the MJO convection for 1∼2 weeks in phase 3, in which the enhanced convective activity exists over the Indian Ocean. While a significantly positive SAT anomaly appears over the same region following the MJO phase 7, as the tropical heating shows an opposite sign. Analysis of the anomalous circulation indicates that the observed SAT signal is probably a result of the northeastward propagating Rossby wave train triggered by MJO-related tropical forcing through Rossby wave energy dispersion. By using an anomalous atmospheric general circulation model (AGCM), the significant effect of tropical forcing on organizing the extratropical circulation anomaly is confirmed. Analysis of a temperature tendency equation further reveals that the intraseasonal SAT anomaly is primarily attributed to the advection of the mean temperature by the wind anomaly associated with the anomalous circulation of the MJO-related variability.     

An ocean wind-wave climatology for the Southern Brazilian Shelf. Part I: Problem presentation and model validation

Geomorphological features and sparse visual wave measurements, presented in previous works, point out to the possible existence of alongshore wave energy gradients over the Southern Brazilian Shelf (SBS). After describing the two numerical models utilized and the model grids and settings, the present study evaluated the proposed validation of the basin-scale results against orbital altimetry and the regional-scale results against the two available wave-buoy data, discussing the extent to which the model reproduced local reality. The basin-scale model results of significant wave height could be considered as in good agreement with observations, presenting remarkable similarities with observed altimetry. Regarding the regional-scale modeling, both significant wave height and peak wave periods were considered as in good agreement with observations. The peak wave directions, however, were classified as in poor agreement, once the ESE waves were erroneously reproduced as E. Increased spatial resolution certainly played a role, but it was the lowering of bottom friction that represented the major improvement in the coastal grid simulations.     

利用废旧轮胎防治滚石的数值模拟分析

为克服传统刚性拦石结构体系,刚性过大,受落石冲击易破坏,场地适应性差等缺点,设想将汽车废旧轮胎堆栈于刚性拦石结构前,形成一种刚柔并重的结构体系。利用废旧轮胎的弹性和韧性缓解落石的冲击力,从而提高传统刚性拦石结构体系防护落石的能力。并通过利用LS-DYNA有限元软件对废旧轮胎在落石冲击作用下的耗能性能进行了分析,计算结果表明废旧轮胎具有较好的吸能效果,对提高刚性拦石结构体系的防护能力有很大的帮助。     

A Boussinesq-type wave driver for a morphodynamical model to predict short-term morphology

The prediction of near-shore morphology on the time scale of a storm event and the length scale of a few surf zone widths is an active area of research. Intense wave breaking drives offshore-directed currents (undertow) carrying sediment seawards, resulting in offshore bar migration. In contrast, higher order nonlinear properties, such as wave asymmetry (velocity skewness) and velocity asymmetry, are drivers for shoreward transport. These wave processes are included in phase-resolving models such as Boussinesq-type wave models (e.g., TRITON). Short-wave averaging in the wave model yields wave-induced forces (e.g., radiation stress gradients) and a wave asymmetry term. The wave-induced forces are used in a hydrostatic model (e.g., Delft3D flow module) to drive the current and undertow, resulting in a 3D velocity profile. The wave model and hydrostatic model are coupled online with a morphodynamic model (e.g., Delft3D morphology module). The latter computes, based on the 3D flow profile and the wave asymmetry term, the sediment transport and performs the bathymetry updates. The updates are transferred directly back to the hydrodynamic models. The coupling of the wave model TRITON and the Delft3D modules is validated by comparing against extensive laboratory data sets (LIP and Boers) and a field case (Duck94), and show a good performance for the hydrodynamics and a reasonable/fair performance for the bar movements.     

Wave types of landslide generated impulse waves

Subaerial landslide generated impulse waves were investigated in a prismatic wave channel. Seven governing parameters, namely the still water depth, slide impact velocity, slide thickness, bulk slide volume, bulk slide density, slide impact angle, and grain diameter, were systematically varied. The generated impulse waves are nonlinear, intermediate- to shallow-water waves involving a small to considerable fluid mass transport. The Stokes wave, cnoidal wave, solitary wave, and bore theories were applied to describe the observed maximum waves. The theoretical and observed features of these four wave types are highlighted. A diagram allows to predict the wave type directly as a function of the slide parameters, the slide impact angle, and the still water depth.     

Pressure resistance of the corrugated stainless steel membranes of LNG carriers

Corrugated stainless steel membranes have been used as the primary barriers for LNG carriers to reduce thermal stresses at cryogenic temperatures. As the capacity of the LNG cargo is increased, however, the corrugated stainless steel membranes suffer from buckling and collapse due to increased sloshing loads in large-capacity LNG cargo carriers. In this study, the pressure resistance of the corrugated part of the stainless steel membrane was evaluated using a finite element method for better design of the LNG containment system, and an experimental equipment was developed and the pressure resistance of the stainless steel corrugations was measured and compared with the calculated results. In addition, a new membrane with high-pressure resistance and its reinforcing method were developed and evaluated experimentally.     

Hydrodynamic characteristics of semi-immersed breakwater with an attached porous plate

In the present study, wave interaction with a fixed, partially immersed breakwater of box type with a plate attached (impermeable-permeable) at the front part of the structure is investigated numerically and experimentally. The large scale laboratory experiments on the interaction of regular waves with the special breakwater were conducted in the wave flume of Laboratori d’Enginyeria Marνtima (LIM) at Universitat Politecnica de Catalunya (UPC) in Barcelona. Experimental results are compared with numerical results obtained with the use of the Cornell breaking Wave and Structures (COBRAS) wave model. The effects of an impermeable as well as a permeable plate attached to the bottom of the breakwater on its hydrodynamic characteristics (wave transmission, reflection, dissipation, velocity and turbulence kinetic energy) are investigated. Computed velocities and turbulence kinetic energy in the vicinity of the structure indicate the effects of the breakwater with the attached (impermeable/permeable) plate on the flow pattern and the turbulence structure. The attached impermeable plate at the front part of the breakwater enhances significantly the efficiency of the structure in attenuating the incident waves. The permeable plate reduces the efficiency of the structure since wave energy is transmitted through the porous body of the plate. Based on the hydrodynamic characteristics it is inferred that the breakwater with an impermeable plate attached to its bottom is more efficient. The comparison of horizontal and vertical forces acting on the breakwater for all cases examined reveals that plate porosity influences slightly vertical force and severely horizontal force acting on the structure, reducing maximum values in both cases.