鬼手海葵(Aiptasia pulchella)白化恢复后的基因表达差异

海葵白化是由于海葵失去体内共生的虫黄藻和(或)共生的虫黄藻失去体内色素而导致海葵变白的生态现象。为探究鬼手海葵(Aiptasia pulchella)白化和白化恢复后相关的分子机制, 本研究对海葵进行慢性热胁迫处理, 以白化海葵和白化恢复后的海葵为研究对象, 采用2代IIlumina Hi-seq测序技术进行转录组测序, 探究两者在基因表达水平方面的差异变化, 分别获得50109686和43163786条Clean reads, 注释后获得24565和24157个Unigene。比较转录组分析结果显示, 白化海葵与白化恢复后的海葵之间存在214个差异表达基因, 其中白化海葵的高表达基因有101个, 白化恢复后海葵的高表达基因有113个, 这些差异表达基因主要与DNA复制、新陈代谢、离子转运和胶原蛋白有关。对不同处理所涉及的全部28050个表达基因进行基因集富集分析(gene set enrichment analysis, GSEA), 结果发现, 白化海葵在胶原蛋白和离子转运方面显著富集表达, 白化恢复后的海葵在核酸修复方面显著富集表达, 推测这些生物学过程在海葵白化和白化后修复过程中发挥重要作用。本研究获得的转录组数据和研究结果初步揭示了海葵共生体系在共生失衡后进行适应性调节的分子机制, 为海葵及珊瑚共生体系应对环境变化的适应性机制研究提供了理论依据。     

近50年涠洲岛5次珊瑚冷白化的海洋站SST指标变化趋势分析

根据涠洲岛海洋站实测海表温度(SST)历史资料和涠洲岛珊瑚礁普查历史资料,采用对比分析与相关分析方法,分析1960―2009年冬季珊瑚冷白化4种SST指标的变化特征。结果显示:(1)涠洲岛珊瑚冷白化的该站SST值为极端低海温(SSTMIN)≤13.2℃或最冷周海温(SSTMCW)≤14.0℃;(2)4种珊瑚冷白化SST指标呈准同步变化,无上升趋势,且其变化趋势与全球变暖无明显对应关系。     

Excess seawater nutrients,enlarged algal symbiont densities and bleaching sensitive reef locations: 2. A regional-scale predictive model for the Great Barrier Reef,Australia

A spatial risk assessment model is developed for the Great Barrier Reef (GBR, Australia) that helps identify reef locations at higher or lower risk of coral bleaching in summer heat-wave conditions. The model confirms the considerable benefit of discriminating nutrient-enriched areas that contain corals with enlarged (suboptimal) symbiont densities for the purpose of identifying bleaching-sensitive reef locations. The benefit of the new system-level understanding is showcased in terms of: (i) improving early-warning forecasts of summer bleaching risk, (ii) explaining historical bleaching patterns, (iii) testing the bleaching-resistant quality of the current marine protected area (MPA) network (iv) identifying routinely monitored coral health attributes, such as the tissue energy reserves and skeletal growth characteristics (viz. density and extension rates) that correlate with bleaching resistant reef locations, and (v) targeting region-specific water quality improvement strategies that may increase reef-scale coral health and bleaching resistance.     

TBM掘进技术发展及有关工程地质问题分析和对策

通过对大量文献资料和工程实例的研究,论述了TBM(隧道掘进机)近半个世纪发展及其在隧道建设中的应用现状和问题。对大量国内外工程实例分析后发现优先采用TBM掘进技术已成为未来隧道建设总的发展趋势。从岩石力学和工程地质角度对岩石TBM掘进过程中遇到的诸如软岩大变形、突水、岩爆等不良工程地质问题导致卡机或管片变形、破损等工程事故进行了剖析,并对采用的工程处理措施作了总结对比。随着今后TBM掘进技术在我国大规模应用,对复杂工程地质条件的客观全面认识和施工方案的及时调整成为影响施工安全和经济效益的关键因素。     

云南金平亚拉寨镍矿矿石组份及其综合利用

亚拉寨镍矿属岩浆熔离型与晚期岩浆结晶分异型复合的硫化物矿床。矿体赋存于基性～超基性杂岩体底部,有用元素镍平均0.44ω%,伴生铜、钴等。对矿石组份及其赋存状态研究后,提出综合利用建议。     

劣质宝石的人工处理

本文简介劣质宝石的人工处理方法,有染色法、漂白法、加热法,涂层法和辐射法等方法,而且尚附有主要宝石常用的处理方法一览表。     

Environmental concerns of poor households in low-income cities: the case of the Tamale Metropolis,Ghana

The Tamale Metropolitan Area (TMA), as a low-income city in a heavily indebted poor country, is at the first stage of the urban environmental transition where most of the environmental problems tend to occur close to the home. Some of the more severe household environmental problems are poor housing, inadequate potable water supply, unsanitary conditions, uncollected garbage, indoor air pollution and pest infestation. Those usually exposed to these environmental burdens are the less wealthy households who have benefited less from development planning and infrastructure provision. Using questionnaire survey and focus group discussions, this study explored the environmental anxieties of households in the metropolis. A stratified sample of residential areas of the city was employed, and the study is able to examine city-wide disparities. The results indicate that problems of water supply are the concern of all groups. Sanitation and garbage disposal services are problems faced mainly by the poor in low-income areas. Other problems faced by the poor are overcrowding, indoor air pollution and pest infestation, but these problems are not highlighted by the poor reflecting a misplaced lack of concern for these problem areas and ignorance of the health risks posed by these hazards. The finding suggests a considerable demand for improvements in environmental service provision and a general willingness to pay for such improvements.

年轻冰川沉积砾石的晒退特征及其对岩石释光埋藏测年的启示

不完全晒退/晒退不均现象是冰川沉积物释光测年所面临的巨大挑战,这一问题限制了传统颗粒沉积物释光方法在冰川沉积物测年方面的应用。最新发展的岩石释光埋藏测年技术,可以根据释光-深度曲线判断砾石是否经历过充分曝光,是解决冰川沉积晒退不均问题的有效途径之一。本工作选择青藏高原东部硬普沟现代冰川前沿为研究对象,采集130个年轻冰川沉积砾石样品,包括93个现代样品和37个小冰期样品,其中20个现代样品采自地表。首先,运用表_{~2 mm}/饱和信号法区分不同冰川沉积环境中砾石样品的晒退程度;再基于SAR和SGC法初步估算样品的等效剂量及其高估情况;然后,统计砾石样品的特征（磨圆度、球度、颜色、晶粒大小、晶粒相对大小、砾石大小等）与晒退程度的关系,找寻晒退良好的砾石及其特征。结果表明大部分采样点的砾石样品呈现了被晒退的特征,平均20.6%的埋藏砾石的岩芯晒退良好,剂量高估程度低;不同沉积类型样品的晒退程度不一,侧碛垄顶部砾石样品晒退程度最好,冰水阶地和现代冰河砾石样品次之,现代冰川后退碛垄间洼地的冰水沉积砾石样品晒退程度最差,剂量高估较严重;磨圆度较差、球度较低、浅色的花岗岩砾石晒退程度较好。综上,冰川沉积砾石的晒退程度与沉积环境、沉积过程、搬运距离、砾石特征等相关,在进行岩石释光埋藏测年采样时,应综合考虑上述情况。     

Luminescence dating of glaciolacustrine silt in overdeepened basin fills beyond the last interglacial

OSL and IRSL dating are applied to samples from a 152 m-long drill core to constrain the timing of three glaciolacustrine depositional periods within the infill of an overdeepened bedrock trough in the Lower Glatt valley, N Switzerland. The characterisation of the dose-response suggests that the polymineral IRSL₅₀ and pIRIR_180/225 signals are close to saturation, while quartz OSL ages are within the range of reliable dating. The demarcation of the upper quartz OSL dating limit, however, remains challenging. Dose-recovery tests performed with long storage periods were used to investigate the reliability of the high region of the dose-response curve. They suggest an upper limit for reliable dating of ∼400 Gy for these samples, which was considerably lower than the commonly used 2D₀ criterion. Lifetimes were calculated for the quartz OSL and the thermal stability of the signal is not considered as problematic for the determined ages. Allowing for a contribution from inherited dose due to partial bleaching, places the infill of the overdeepened valley within the penultimate glacial cycle (MIS6).     

Coral mass bleaching and reef temperatures at Navassa Island, 2006

Bleaching and associated mortality is an extreme threat to the persistence of coral populations in the projected warming regime of the next few decades. Recent evidence indicates that thermal bleaching thresholds may be affected by water quality gradients. The unexpected encounter of a coral mass bleaching event at a remote, uninhabited Caribbean island (Navassa) during a routine reef assessment cruise in November 2006 provided the opportunity to characterize bleaching responses and thermal exposure in an oceanic area with negligible continental influence or human impact on water quality. The coral taxa most susceptible to bleaching were Agaricia spp. and Montastraea faveolata. Siderastraea siderea, Diploria spp. and Porites porites were intermediately affected, while Porites astreoides and Montastraea cavernosa were minimally affected and negligible bleaching was observed in Acropora palmata. Bleaching prevalence (colonies > 4 cm diameter) ranged from 0.16 to 0.63 among sites. Deeper sites (between 18 and 37 m) had significantly higher prevalence of bleaching than shallow sites (<10 m). This general pattern of more bleaching in deeper sites also occurred within species. Though exposure to high-temperature stress was not greater at deeper sites, water motion, which may bolster bleaching resistance, was likely less. In situ loggers indicated temperatures over 30 °C initiated at shallow sites in mid-August, at deeper sites in early September, and were persistent at all sites until mid-October. Long term (1983–2007) climatologies constructed from AVHRR SSTs suggest that the mass bleaching event observed at Navassa in 2006 corresponded with greater intensity and duration of warm temperature anomalies than occurred in 2005, for which no in situ observations (bleaching nor temperature) are available.