Isolation and identification of a bacterium from marine shrimp digestive tract: A new degrader of starch and protein

It is a practical approach to select candidate probiotic bacterial stains on the basis of their special traits. Production of digestive enzyme was used as a trait to select a candidate probiotic bacterial strain in this study. In order to select a bacterium with the ability to degrade both starch and protein, an ideal bacterial strain STE was isolated from marine shrimp (Litopenaeus vannamei) intestines by using multiple selective media.The selected isolate STE was identified on the basis of its morphological, physiological,and biochemical characteristics as well as molecular analyses. Results of degradation experiments confirmed the ability of the selected isolate to degrade both starch and casein. The isolate STE was aerobic, Gram-negative, rod-shaped, motile and non-spore-forming, and had catalase and oxidase activities but no glucose fermentation activity. Among the tested carbon/nitrogen sources, only Tween40, alanyl-glycine, aspartyl-glycine, and glycyl-l-glutamic acid were utilized by the isolate STE. Results of homology comparison analyses of the 16S rDNA sequences showed that the isolate STE had a high similarity to several Pseudoalteromonas species and, in the phylogenetic tree, grouped with P. ruthenica with maximum bootstrap support (100%). In conclusion, the isolate STE was characterized as a novel strain belonging to the genus Pseudoalteromonas.This study provides a further example of a probiotic bacterial strain with specific characteristics isolated from the host gastrointestinal tract.     

与翡翠伴生的含钠长石质玉石的鉴别及定名探讨

与翡翠伴生的含钠长石质玉石的外观与传统翡翠非常相似,但成分比较复杂,宝石学参数不再典型,性质常介于钠长石玉和翡翠之间。通过对标本的观察、常规宝石学测试和红外光谱测试,对该过渡类型玉石进行仔细区域调查,总结特征,建议国家标准应加强对翡翠与岩石过渡类型具体的成分标准和物理性质的归钠总结,对相应的类型划分与种类界定提出规范性和统一的标准。     

基于FY-4A卫星数据的强对流云团识别与监测

为解决强对流监测问题,克服地区亮温特征对卫星监测的影响,利用FY 4A卫星L1数据,结合滑动窗口方法和多通道动态阈值自动识别法,对典型强对流云团进行识别与监测。结果表明：1）多通道动态阈值自动识别方法结合滑动窗口方法,可避免人为设置阈值的主观性,整合强对流的区域识别结果,实现全国强对流云团监测。2）此方法具有良好的强对流云团识别效果,识别正确率达到89%;3）FY 4A卫星识别结果与雷达反射率高值区基本一致,能够准确监测强对流云团发生发展和移动的过程,具有较高的识别精度。     

A coupled damage–plasticity model for concrete based on thermodynamic principles: Part I: model formulation and parameter identification

The development of a coupled damage‐plasticity constitutive model for concrete is presented. Emphasis is put on thermodynamic admissibility, rigour and consistency both in the formulation of the model, and in the identification of model parameters based on experimental tests. The key feature of the thermodynamic framework used in this study is that all behaviour of the model can be derived from two specified energy potentials, following procedures established beforehand. Based on this framework, a constitutive model featuring full coupling between damage and plasticity in both tension and compression is developed. Tensile and compressive responses of the material are captured using two separate damage criteria, and a yield criterion with a multiple hardening rule. A crucial part of this study is the identification of model parameters, with these all being shown to be identifiable and computable based on standard tests on concrete. Behaviour of the model is assessed against experimental data on concrete. Copyright © 2007 John Wiley & Sons, Ltd.     

A VIBRIO ANGUILLARUM STRAIN ASSOCIATED WITH SKIN ULCER ON CULTURED FLOUNDER, PARALICHTHYS OLIVACEUS

ＩＮＴＲＯＤＵＣＴＩＯＮＩｎｃｒｅａｓｉｎｇａｔｔｅｎｔｉｏｎｗａｓｆｏｃｕｓｅｄｏｎｔｈｅｎｅｅｄｔｏｓｔｕｄｙｔｈｅｂａｃｔｅｒｉｏｌｏｇｙｏｆｍａｒｉｎｅｆｉｓｈｉｎＣｈｉｎａ ,ｂｅｃａｕｓｅｏｆｂａｃｔｅｒｉａａｓｓｏｃｉａｔｉｏｎｗｉｔｈｈｉｇｈｍｏｒｔａｌｉｔｙｉｎｍａｒｉｎｅｆｉｓｈｃｕｌｔｕｒｅａｎｄｃｏｎｓｅｑｕｅｎｔｅｃｏｎｏｍｉｃｌｏｓｓｔｏｆｉｓｈｆａｒｍｓ (Ｄｅｎｇｅｔａｌ.,1 996;Ｌｉｎｅｔａｌ.,1 999) .Ｖｉｂｒｉｏｇｅｎｕｓｂａｃｔｅｒｉａｈａｖｅｅｍｅｒｇｅｄａｓｔ…     

A VIBRIO ANGUILIARUM STRAIN ASSOCIATED WITH SKIN ULCER ON CULTURED FLOUNDER, PARALICHTHYS OLIVACEUS

The characteristics of a bacterium strain M3, isolated from cultured flounderParalichthys olivaceus with remarkable external sign of skin ulcer during an epizootic outbreak, indicated that the bacterium belonged to the speciesVibrio anguillarum. Challenge by I.M. (intramuscular injection), bath, and oral administration with M3 showed that it was highly pathogenic forParalichthys olivacues. The LD₅₀ dose was 5.144×10³ CFU/ per fish infection by I.M. injection. Recovered inoculated bacteria from the surviving fish revealed that the asymptomatic carriers could be a latent contagious source. Study of the effect of bacterial culture CFS (cell-free-supernatant) showed that the exotoxins produced by M3 play an important role in its pathogenicity for flounder. The resistance of M3 to 36 out of 41 antibiotics indicated that the bacterial disease outbreak was mainly attributable to the frequent and excessive use of antimicrobial agents; and that vaccination would be an effective precaution against bacterial disease. Contribution No. 4107 from Institute of Oceanology, Chinese Academy of Science. Contribution No. 270 from Experimental marine Biology Laboratory, CAS. Funded by projects under the Major State Basic Research Development Program, Grant G1999012003.     

A local identification technique for geotechnical and geophysical systems

Geotechnical structures and natural soil deposits are massive multi‐phase particulate systems characterized by the development of localized response mechanisms under extreme loading conditions. Identification and analysis of such systems based on inverse boundary value problem formulations and sparse measurements are generally indeterminate. This paper presents an alternative local inverse problem technique. Point‐wise identification analyses of the constitutive behaviour of water‐saturated geotechnical and geophysical systems are performed using acceleration and pore pressure records provided by a cluster of closely spaced sensors. The developed novel technique does not require the availability of boundary condition measurements, or solution of an associated boundary value problem. The constitutive behaviour at a specific location of a soil‐system is analysed independently of adjacent response mechanisms or material properties. Numerical simulations and centrifuge tests of a soil‐retaining wall system are used to demonstrate the capabilities of the developed technique. Copyright © 2003 John Wiley & Sons, Ltd.     

Assessing the germplasm of Laminaria (phaeophyceae) with random amplified polymorphic DNA (RAPD) method

Eighteen gametophytes includingL. japonica, L. ochotensis andL. longissima, were verified with random amplified polymorphic DNA (RAPD) technique. Eighteen ten-base primers were chosen from 100 primers selected for final amplification test. Among the total of 205 bands amplified, 181 (88.3%) were polymorphic. The genetic distance among different strains ranged from 0.072 to 0.391. The dendrogram constructed by unweighted pair-group method with arithmetic (UPGMA) method showed that the female and male gametophytes of the same cell lines could be grouped in pairs respectively. It indicated that RAPD analysis could be used not only to distinguish different strains ofLaminaria, but also to distinguish male and female gametophyte within the same cell lines. There is ambiguous systematic relationship if judged merely by the present data. It seems that the use of RAPD marker is limited to elucidation of the phylogenetic relationship among the species ofLaminaria. Contribution No. 4389 from the Institute of Oceanology, Chinese Academy of Sciences. This research was supported by the State's High-tech Project of China, Marine 863 project (No. 819-03-02) and (2001AA621090).     

Atlas of M7III Spectral Features in the Optical

The difficulties encountered in attempts to study the spectra of very late stars inspired us to prepare this spectral atlas containing essentially spectral features of M7III stars in the optical spectral region. As representative star we took the cool component of the binary symbiotic CH Cygni during one of its quiescent phases. Total number of 920 atomic lines and molecular bands have been identified with different degrees of reliability. This revised version was published online in July 2006 with corrections to the Cover Date.     

A high-accuracy computed water line list

A computed list of  H₂¹⁶O  infrared transition frequencies and intensities is presented. The list, BT2, was produced using a discrete variable representation two-step approach for solving the rotation–vibration nuclear motions. It is the most complete water line list in existence, comprising over 500 million transitions (65 per cent more than any other list) and it is also the most accurate (over 90 per cent of all known experimental energy levels are within 0.3 cm⁻¹ of the BT2 values). Its accuracy has been confirmed by extensive testing against astronomical and laboratory data.
The line list has been used to identify individual water lines in a variety of objects including comets, sunspots, a brown dwarf and the nova-like object V838 Mon. Comparison of the observed intensities with those generated by BT2 enables water abundances and temperatures to be derived for these objects. The line list can also be used to provide an opacity for models of the atmospheres of M dwarf stars and assign previously unknown water lines in laboratory spectra.