建鲤与黄河鲤的RAPD分子标记及其杂交优势的遗传分析

利用RAPD技术对建鲤、黄河鲤及其杂交子代进行了遗传分析,筛选的33个引物共扩增出155条带,其中多态性片段为105条,片段S18-1600、S472-300具有种的特异性,可作为鉴别建鲤与黄河鲤的分子遗传标记。建鲤、黄河鲤及其正、反交子代群体内的遗传相似系数分别为0.8240、0.7921、0.7920、0.8569,黄河鲤与正交F1具有较高的遗传变异水平,反交F1变异最小。正交F1、反交F1与亲本(建鲤、黄河鲤)的遗传距离分别为0.2233、0.2436、0.1749、0.2026,说明子代均继承了较多的建鲤的遗传物质。分析了子代与亲本的RAPD标记类型以及各类型的条带数。应用PIT标记系统测定了子代的杂种优势并探讨了优势产生的遗传机理。     

Dyadic wavelet analysis of PDA signals

The dyadic wavelet transform is used to analyze PDA measured signals in order to identify the CASE-damping factor, which may be directly calculated from the dyadic wavelet analysis, not from the correlation study; accordingly, the pile capacity may be more exactly estimated by the CASE method. The dyadic wavelet transform can decompose a PDA measured signal into an incident impact wave and a reflected impulse wave at the certain scale that are clearly shown on the wavelet transform graph. The relation between the incidence and the reflection has been established by a transfer function based on the dyadic wavelet transform and the one-dimensional wave equation, whose phase is the time delay between the incident and the reflected and whose magnitude is a function of the CASE-damping factor. An autocorrelation function analysis method is proposed to determine the time delay and to estimate the magnitude of the transfer function that is determined by the ratio of the maximum of the autocorrelation function to the second peak value represented the reflected wave on the autocorrelation function graph. Thus, the damping factor is finally determined. An analog signal, a PIT signal and five PDA signals demonstrate the proposed methods, by which the time delay, the CASE-damping factor, and pile capacity are determined. The damping factors and pile capacity are good agreement with those by CAPWAP.