蛤蜊科3种贝类16SrRNA基因片段及ITS2核苷酸序列分析

利用PCR技术分别扩增连云港及启东沿海蛤蜊科的西施舌（Coelomactra antiquata）、中国蛤蜊（Mactra chinensis）和四角蛤蜊（Mactra veneriformis）3种双壳贝的16SrRNA基因片段和ITS2核苷酸序列．测序后用DNAstar软件分析了核苷酸差异。结果显示：三种贝类16SrRNA基因片段长度相同，均为306bp（去除引物）．核苷酸存在多态性。共有45个变异位点，54个核苷酸发生了变异。全部为碱基置换。西施舌与中国蛤蜊此片段核苷酸的同源性为88．9％．与四角蛤蜊的同源性为88．6％．中国蛤蜊与四角蛤蜊的同源性为90．6％。三种蛤蜊ITS2序列分别为390bp（西施舌）、441bp（四角蛤蜊）和466bp（中国蛤蜊）。存在长度多态性．ITS2核苷酸差异分析结果显示．西施舌与中国蛤蜊的同源性为70．9％-71．1％，西施舌与四角蛤蜊的为70．5％-71．0％。中国蛤蜊与四角蛤蜊的同源性为88．1％-88．8％。ITS2序列分析结果与16SrRNA基因片段分析结果一致．2种分子分析法均显示中国蛤蜊与四角蛤蜊的亲缘关系近。     

Hydrated sulphuric acid in dense molecular clouds

We consider sulphur depletion in dense molecular clouds, and suggest hydrated sulphuric acid, H₂SO₄ ·  n H₂O, as a component of interstellar dust in icy mantles. We discuss the formation of hydrated sulphuric acid in collapsing clouds and its instability in heated regions in terms of the existing hot core models and observations. We also show that some features of the infrared spectrum of hydrated sulphuric acid have correspondence in the observed spectra of young stellar objects.     

Non-Sobolev modelling of radiation-pressure-driven flows in active galactic nuclei

We present a new general scheme for calculating the structure and dynamics of radiation-pressure-driven photoionized flows. The new method goes one step beyond the Sobolev approximation. It involves a numerical solution of the radiative transfer in absorption lines, including the effects of differential expansion and line interactions such as line locking and blanketing. We also present a new scheme for calculating the radiation pressure due to trapped line photons in finite, differentially expanding flows. We compare our results for the radiation pressure force with those obtained using the Sobolev approximation and show the limitations of the latter. In particular, we demonstrate that the Sobolev method gives a poor approximation near discontinuity surfaces and its neglect of line blanketing can lead to erroneous results in high-velocity flows. We combine the newly calculated radiation pressure force with self-consistent photoionization and thermal calculations to study the dynamics and spectral features of broad absorption-line flows and highly ionized gas flows in active galactic nuclei (AGN). A comparison with Sobolev-type calculations shows that the latter overestimates the terminal velocity of the flow and, conversely, underestimates its opacity. We also show that line locking on broad emission lines can have a significant effect on the dynamics and spectral features of AGN flows.     

SPECULATION ON DEVELOPING TOUR RESOURCES OF THE TOURNAMENT ATHLETICS IN HARBIN CITY

The exploitation and utilization of the tour resources of tournament athletics, including skiling, boat sail-ing, archery, ice engraving, snow engraving, has become a new trend of the development of Chinese tourism. Due tothe unique cold climate and superior geographic location, Harbin is a promising city for developing tour resources of tourna-ment athletics. Based on the analysis of the superiority and peculiarity of Harbin, the speculation on development of tour resources of tournament athletics in Harbin is proposed as follows : 1 ) Harbin should develop its special tour resources of tournament athletics associated with needs of market; 2) Harbin should take the advantages of rich resources and devel-op ice and snow entertainment in winter and travel for sight-seeing and spending summer;, 3) the adjustment of the layoutof ice and snow resources should be based on the idea of taking Harbin as the center and all-side opening at the largescale in the way of radiation; 4) tourism should be developed by the combined efforts of various departments to make feasi-ble plan, and the organizers should pay much attention to ensuring the safetv of tourists.     

The response of an elastic half‐space under a momentary shear line impulse

The response of an ideal elastic half‐space to a line‐concentrated impulsive vector shear force applied momentarily is obtained by an analytical–numerical computational method based on the theory of characteristics in conjunction with kinematical relations derived across surfaces of strong discontinuities. The shear force is concentrated along an infinite line, drawn on the surface of the half‐space, while being normal to that line as well as to the axis of symmetry of the half‐space. An exact loading model is introduced and built into the computational method for this shear force. With this model, a compatibility exists among the prescribed applied force, the geometric decay of the shear stress component at the precursor shear wave, and the boundary conditions of the half‐space; in this sense, the source configuration is exact. For the transient boundary‐value problem described above, a wave characteristics formulation is presented, where its differential equations are extended to allow for strong discontinuities which occur in the material motion of the half‐space. A numerical integration of these extended differential equations is then carried out in a three‐dimensional spatiotemporal wavegrid formed by the Cartesian bicharacteristic curves of the wave characteristics formulation. This work is devoted to the construction of the computational method and to the concepts involved therein, whereas the interpretation of the resultant transient deformation of the half‐space is presented in a subsequent paper. Copyright © 2003 John Wiley & Sons, Ltd.     

Can gas dynamics in centres of galaxies reveal orbiting massive black holes?

If supermassive black holes in centres of galaxies form by merging of black hole remnants of massive Population III stars, then there should be a few black holes of mass one or two orders of magnitude smaller than that of the central ones, orbiting around the centre of a typical galaxy. These black holes constitute a weak perturbation in the gravitational potential, which can generate wave phenomena in gas within a disc close to the centre of the galaxy. Here, we show that a single orbiting black hole generates a three-arm spiral pattern in the central gaseous disc. The density excess in the spiral arms in the disc reaches values of 3–12 per cent when the orbiting black hole is about 10 times less massive than the central black hole. Therefore, the observed density pattern in gas can be used as a signature in detecting the most massive orbiting black holes.     

Virtual Huanghe River System： Framework and Technology

1 Introduction Huanghe (Yellow) River basin is located in 32°–42°N, 96°–119°E. The area of the catchment is more than 752,000km2. The river is 5464km long with a drop in elevation of 4830m. Among the whole area, the moun- tainous and stone area accounts for 29%, loess and hills area 46%, sandy area 11% and plain area 14%, respec- tively. Different natural landscapes exist in this area. The Huanghe River flows through the Loess Plateau, where the soil is eroded seriously (Wang, 2002;…