凡纳滨对虾P23蛋白基因的筛选、表达和生物信息学分析

克隆凡纳滨对虾极端体重个体的组织差异表达基因P23基因并进行生物信息学分析, 为进一步研究该基因的功能以及凡纳滨对虾的分子选育等研究提供信息。以凡纳滨对虾雌虾极端体重个体腹部肌肉为实验材料, 利用抑制消减杂交(SSH)技术构建极大体重雌性个体和极小体重雌性个体腹部肌肉组织正反向消减cDNA文库:正库(以极大体重个体为试验组, 以极小体重个体为驱动组, L-S)和反库(以极小体重个体为试验组, 以极大体重个体为驱动组, S-L)消减cDNA文库, 并采用实时定量技术分析P23基因的组织表达规律, 利用生物信息学对其功能和结构进行预测。以β-actin为看家基因检测两个文库的消减效率分别为210和25, 实时定量技术分析结果表明P23基因在体重的调节中起上调作用。P23基因编码区序列全长495bp, 编码165个氨基酸, GenBank登录号:JF806619。生物信息学分析发现P23蛋白部分序列含有强疏水性, 无跨膜螺旋结构, 两种信号肽预测都显示P23含有信号肽。     

基于T/P 和Jason-1 高度计数据的渤黄东海潮汐信息提取

对19 a 的TOPEX/POSEIDON(以下称T/P)和Jason-1 卫星高度计测高数据进行调和分析, 得到渤黄东海海域的8 个主要分潮(M2、S2、N2、K2、K1、O1、P1 和Q1)。提出一种将两类卫星高度计数据统一的方法, 消除了因两类卫星高度计校正算法等不同所导致的相互之间的偏差。变轨后的T/P与Jason-1 卫星加密了高度计对潮汐观测的空间分布。通过对交叉点处升轨与降轨的潮汐调和分析结果进行比较, 检验调和分析方法及高度计数据的可靠性; 将基于高度计数据的调和分析结果与验潮站资料进行比较, 以检验其正确性。4 个主要分潮(M2、S2、K1、O1)振幅之差的均方根介于1.0~1.8 cm, 迟角之差的均方根介于4.1°~7.8°。与已有研究结果相比, 调和分析结果的精确性有所提高。在此基础上, 综合变轨前后两类高度计测高数据的调和分析结果, 给出并分析了渤黄东海4 个主要分潮的同潮图。     

Photosynthetic performance,epiphyte biomass and nutrient content of two seagrass species in two areas with different level of nutrients along the Dar es Salaam coast

Heavy nutrient loads in coastal waters often lead to excessive growth of microalgal and macroalgal epiphytes on seagrass leaves, with varying effects on the underlying seagrasses. This study evaluates the photosynthetic performance, epiphytic biomass and tissue nutrient content of two tropical seagrasses, Cymodocea serrulata and Thalassia hemprichii, in two intertidal areas along the Dar es Salaam coast in the Indian Ocean, a nutrient-rich region at Ocean Road (near the city centre), and a nutrient-poor region at Mjimwema (south of the city centre). Epiphyte biomass was significantly higher at the nutrient-rich site, and epiphytes were associated with reduced photosynthetic performance in both seagrass species at both sites. Likewise, nitrogen and phosphorus tissue content was higher in both species at the nutrient-rich site than at the nutrient-poor site. Epiphytic species composition on the seagrass leaves varied between seagrass species and between sites. Cymodocea serrulata had a higher number of epiphytic species at Mjimwema than at Ocean Road, whereas Thalassia hemprichii had more epiphytic species at Ocean Road than at Mjimwema. Seagrass photosynthetic performance, epiphytic biomass and nutrient content of the seagrasses were shown to be affected by nutrient concentration in the water column. Thus, for the future monitoring of the seagrass meadow, we recommend the use of combined measures such as seagrass performance, epiphytic biomass, nutrient contents and nutrient concentration levels in the water column.     

P波作用下饱和土中输流管道动力响应

根据Biot波动理论,采用复变函数与多级坐标法,求解了P波作用下饱和土体中地下输流管道的波动散射方程,分析了管道中流体介质性质、入射波角度及管道埋深等对地下输流管道周边动应力集中系数及孔压集中系数分布的影响。计算结果表明：在低频弹性波入射时,管道周边动应力集中系数及孔压集中系数分布相对均匀,而随入射频率的增加,其分布变得复杂化,但其峰值有所减小;中低频波入射作用下,管道内为水、石油介质时,动应力集中系数和孔压集中系数较空气介质时小,而在高频波作用时情况相反;对于管道内流体介质为水时,随着入射角度的变化,应力集中分布也沿一定角度的方向发生转动,入射波自管道下方垂直入射时,管道周边动应力集中系数峰值相对较大;随埋深的增加,动应力集中系数和孔压集中系数均呈震荡减小的趋势。     

Identification of Cytochrome P450 (CYP) genes in Zhikong scallop (Chlamys farreri)

Cytochrome P450 (CYP) superfamily is one of the membership largest and function most diverse protein superfamily recogniozed among living beings. Members of this superfamily were further assigned to different families and subfamilies based on their amino acid similarities. According to their phylogenetic relationships, the CYP genes which likely diverged from common ancestor gene and may share common functions were grouped into one clan. Widely distributing scallops are a group of the most conspicuous bivalve; however the studies on their CYP is acarce. In this study, we searched the genome and expressed sequence tags of Zhikong scallop (Chlamys farreri) for CYP genes. In total, 88 non-redundant CYP were identified, which were homed in 13 CYPs gene families. Phylogenetic analysis divided these genes into 4 CYP clans. As in deuterostomes, Clan 2 was the largest, which contained 33 genes belonging to CYP1, CYP2, CYP17 and CYP356 families. Clan 3 contgained 19 genes belonging to CYP3, CYP5 and CYP30 families. Clan 4 contained 23 genes, all belonging to CYP4 family. The mitochondrial CYP clan contained 9 genes belonging to CYP10 and CYP24 families. In comparison, protostomes (C. farreri, D. pluex, D. melanogaster) contained more CYP genes than deuterostomes (S. purpuratus and vertebrates) in Clan 2 but less genes in Clan 3 and Clan 4. Our findings will aid to deciphering CYP function and evolution in scallops and bivalves.     

CLONGING AND THE EXPRESSION ANALYSIS OF CYP19A GENE cDNA DURING THE REPRODUCTIVE CYCLE OF SEBASTES SCHLEGELI

从许氏平鲐脑组织中克隆到细胞色素P450芳香化酶（P450aromB）（CYP19B）基因核心功能区cDNA。结果表明,许氏平铀CYP19B主要功能区片段编码369个氨基酸,包括I-螺旋区、Ozol’s肽区及部分芳香化酶特异性保守区。RT-PCR分析表明,CYP19B在雌鱼和雄鱼各组织中表达情况不同,在雌鱼中表达较丰富,但是两者都在性腺和脑中有表达,且脑中的表达量高于性腺。生殖季节表达结果表明,CYP19B在繁殖前期表达较为丰富,退化期未见有表达。CYP19B在精巢处于Ⅲ期的许氏平鲐脑组织中表达最为丰富,在精巢发育Ⅳ期的脑组织表达量最低。上述研究结果说明,CYP19B在雌性和雄性许氏平鲐繁殖周期中均发挥重要生理作用。     

三斑石斑鱼(Epinephelus trimaculatus)两种芳香化酶cDNA的克隆及其表达的组织特异性

通过设计引物,从三斑石斑鱼的卵巢中克隆鉴定出性腺芳香化酶(EtP450arom a)和脑芳香化酶(EtP450arom b)的cDNA序列,EtP450arom a开放阅读框为1557bp,编码519个氨基酸残基;EtP450arom b开放阅读框为1530bp,共编码510个氨基酸残基;性腺芳香化酶(EtP450arom a)和脑芳香化酶(EtP450arom b)的氨基酸序列的同源性为63.3%,并且包含了芳香化酶经典的功能保守结构。构建了芳香化酶的系统进化树。采用半定量RT-PCR方法研究了性腺芳香化酶(EtP450arom a)和脑芳香化酶(EtP450arom b)在雌性成鱼各组织中的mRNA的表达情况。组织表达结果显示,性腺芳香化酶EtP450arom a只在性腺中特异性表达,而脑芳香化酶EtP450arom b主要在脑区和垂体中表达,表明三斑石斑鱼两种芳香化酶基因的组织特异性及功能的差异。     

Inversion of two-dimensional tidal open boundary conditions of M<Subscript>2</Subscript> constituent in the Bohai and Yellow Seas

Two-dimensional tidal open boundary conditions of the M2 constituent in the Bohai and Yellow Seas(BYS) have been estimated by assimilating T/P altimeter data.During inversion,independent point(IP) strategy was used,in which several IPs on the open boundary is assumed,values at these IPs can be optimized with an adjoint method,and those at other grid points are determined by linearly interpolating the values at IPs.The reasonability and feasibility of the model are tested by ideal twin experiments.In the practical experiment(PE) after assimilation,the cost function may reach 1% or less of its initial value.Mean absolute errors in amplitude and phase can be less than 5 cm and 5°,respectively,and the obtained co-chart can show the character of the M2 constituent in the BYS.The results of the PE indicate that using only two IPs on the open boundary can yield better simulated results.     

龙门山断裂带南段地壳一维P波速度结构

基于2009年1月1日至2013年5月6日四川地震台网、重庆地震台网记录的龙门山断裂带南段587个地震的5 012个P波到时数据,利用最小一维速度模型方法反演了龙门山断裂带南段地壳一维P波速度模型及台站校正值,并将其应用到龙门山地区地震重定位中。结果表明,台站校正值表征出龙门山断裂带南段地表速度结构的横向不均匀性,青藏高原的彭灌杂岩体及宝兴杂岩体在近地表表现为高速异常,而四川盆地的第四纪沉积表现为低速异常。重定位后地震震源在北西向的剖面上呈明显的条带状并向北西倾斜,该地震带与宝兴杂岩下方的滑脱带延伸趋势一致。此外,该地震带上方分布着一条反冲地震带,两地震带呈"y"型分布,这可能是宝兴杂岩上方的岩层为调节逆冲过程受阻而产生的反冲运动所致。