A high sensitivity, low volume HPLC method to determine soluble reactive phosphate in freshwater and saltwater

A reverse phase High Performance Liquid Chromatography (HPLC) method was developed to detect soluble reactive phosphate (SRP) in marine and freshwater samples. This technique is scalable over a range of sample volumes from 10 μl to 10 ml, requires minimal sample preparation, and responds linearly from 1 nM to 100 μM (R²>0.964). Standard Murphy–Riley molybdenum blue reagents were used to react with phosphate in samples for analysis. Solid phase extraction tubes were used to clean the mixed complexing reagent prior to combining the reagent with samples. The method was applied to freshwater samples collected in July 2001 along an Andean mountain stream located in the Eastern Cordillera (2400 m) of Peru and marine samples collected in January 2002 within the low marsh of the estuarine Nueces Delta System, Texas Coastal Bend. For method comparison, field samples were analyzed using a standard manual spectrophotometric method. Field sample phosphate concentrations produced from each method were nearly identical. This method offers the advantages of low sample volume (1 ml or less) with high sensitivity, precision, and potential automation.     

大亚湾网箱养殖区生物-化学特性与营养状况的周日变化

根据1998年11月对大亚湾大鹏澳网箱养殖区进行的定点24h连续水质监测资料,分析并讨论了该养殖区海水生物-化学各要素的垂直分布和周日变化规律,并用单项指标评价法和改进的营养状态质量指数(NQI)法,参照营养指数(E)对该网箱养殖区海水的营养状况进行了评价和研究.结果表明,各项要素的平均值大都是表层＞中层＞底层;周日变化主要受潮汐运动和海水中生物的节律性生理活动的综合影响;表层海水的富营养化率明显高于中层海水,其各单项指标处于中度一富营养状态,而中层和底层海水则分别处于贫一中度和贫-富营养状态;PO4-P相对较贫乏,为养殖区富营养化的最关键限制因素.     

北黄海浮游植物营养盐限制的初步研究

于１９９７年１１月,在北黄海采用现场实验的方法,研究营养盐对浮游植的的上行效应。将浮物植的分为３个粒级：网采浮游植物（ｎｅｔｐｈｙｔｏｐｈｌａｎｋｔｏｎ,简称ｎｅｔ,２０－２００μｍ）微型浮植物（ｎａｎｏｐｈｙｔｏｐｌａｎｋｔｏｎ,简称ｎａｎｏ－,２－２０μｍ）和超微型浮游植物（ｐｉｃｏｐｈｙｔｏｐｌａｎｋｔｏｎ,简称ｐｉｃｏ－,〈２μｍ）实验期间ｐｉｃｏ－味绿素ａ的生物量占整个浮的群总叶绿素ａ生     

典型寒区流域水文过程模拟及分析

西北高山区寒区流域在全球变化背景下,河川径流发生了明显的变化,定量分析和评估径流变化对制定西部水资源可持续利用有着重要的意义.在VIC模型（含有冻土计算方案）的框架基础上,成功了耦合能量-物质平衡方案,改善了其在寒区冰川流域的水文过程模拟的效果.选择新疆阿克苏河流域作为研究区,验证表明改进后的模型取得比较理想的模拟结果...     

黄河干流河道水量自然补损分析

根据水量平衡原理建立干流区间河道水量平衡关系,得到干流河道水量自然补损计算方程。利用1956~1979年干流区间自产天然径流量与区间河道上下游控制站天然径流量、区间降水量系列之间的线性相关性,延长得到黄河流域1951~1998年龙羊峡以下6个干流区间逐月自产天然径流量,并计算分析各干流区间的干流河道自然补损水量。结果表明：多年平均河口镇－龙门干流区间和三门峡－花园口干流区间河道水量补充大于损失,其余区间干流河道水量为损失大于补充。总体上,龙羊峡以下区间干流河道净自然损失水量多年平均为35.43×108 m³,而且呈增大趋势,20世纪90年代达到最高,平均为44.16×108 m³/年。     

A comparative study of nutrient transfer via surface runoff from two small agricultural catchments in north China

A field study was conducted to determine the effect of landscape spatial pattern and micro-topography on nutrient transfer via runoff from two catchments into Yuqiao Reservoir in north China. The surface runoff discharge was measured during rainfall events and water samples were analyzed in 2004 and 2005. The mean annual total nitrogen (TN) and total phosphorus (TP) exports per unit area from Caogezhuang catchment (C catchment) were 1.048 and 0.561 kg ha⁻¹ year⁻¹, respectively, while the TN and TP exports from Taohuasi catchment (T catchment) were 0.158 and 0.027 kg ha⁻¹ year⁻¹. In both catchments, village and vineyard shared the highest nutrient export ability due to the accumulated animal waste and heavy application of fertilizer and manure. In T catchment, the distance of village and vineyard was about 1,500 m away from the receiving water and in between were woodland and cropland. In the hydrological pathway, there were sink landscape structures of small stone dams, roadside swale, vegetated filter strip and dry ponds, which could detain water and nutrients. In C catchment, the distance between the village and the receiving water was about 200 m, and the hydrological pathway was compacted roads and ditches with no sink structures. It is suggested that the distance between the pollution source area and the receiving water and the micro-topographical features were the main factors to control the great difference in nutrient export rates.     

东山八尺门海堤西侧网箱养殖海域营养状态初探

本文根据1989年10月和1990年4月在福建东山县八尺门海堤西侧网箱养殖海域现场调查获得的海水化学、浮游植物、叶绿素a等资料,以有机污染指数和营养状态指数为指标,研究了该海域的营养状态,结果表明:1989年4月中旬,该海域有机污染严重,富营养化程度高。底层水呈低氧或无氧状态。推测10月9日夜间网养鱼大量死亡的主要原因是水体缺氧状态加剧所致。     

Biogeochemical transport in the Loxahatchee River estuary, Florida: The role of submarine groundwater discharge

The distributions of dissolved organic carbon (DOC), Ba, U, and a suite of naturally occurring radionuclides in the U/Th decay series (²²²Rn, ^{223,224,226,228}Ra) were studied during high- and low-discharge conditions in the Loxahatchee River estuary, Florida to examine the role of submarine groundwater discharge in estuarine transport. The fresh water endmember of this still relatively pristine estuary may reflect not only river-borne constituents, but also those advected during active groundwater/surface water (hyporheic) exchange. During both discharge conditions, Ba concentrations indicated slight non-conservative mixing. Such Ba excesses could be attributed either to submarine groundwater discharge or particle desorption processes. Estuarine dissolved organic carbon concentrations were highest at salinities closest to zero. Uranium distributions were lowest in the fresh water sites and mixed mostly conservatively with an increase in salinity. Suspended particulate matter (SPM) concentrations were generally lowest (< 5 mg L^− 1) close to zero salinity and increased several-fold ( 18 mg L^− 1; low discharge) toward the seaward endmember, which may be attributed to dynamic resuspension of bottom sediments within Jupiter Inlet.Surface water-column ²²²Rn activities were most elevated (> 28 dpm L^− 1) at the freshwater endmember of the estuary and appear to identify regions of the river most influenced by the discharge of fresh groundwater. Activities of four naturally occurring isotopes of Ra (^{223,224,226,228}Ra) in this estuary and select adjacent shallow groundwater wells yield mean estuarine water-mass transit times of less than 1 day; these values are in close agreement to those calculated by tidal prism and tidal frequency. Submarine groundwater discharge rates to the Loxahatchee River estuary were calculated using a tidal prism approach, an excess ²²⁶Ra mass balance, and an electromagnetic seepage meter. Average SGD rates ranged from 1.0 to 3.8 × 10⁵ m³ d^− 1 (20–74 L m^− 2 d^− 1), depending on river-discharge stage. Such calculated SGD estimates, which must include both a recirculated as well as fresh water component, are in close agreement with results obtained from a first-order watershed mass balance. Average submarine groundwater discharge rates yield NH₄⁺ and PO₄^− 3 flux estimates to the Loxahatchee River estuary that range from 62.7 to 1063.1 and 69.2 to 378.5 μmol m^− 2 d^− 1, respectively, depending on river stage. SGD-derived nutrient flux rates are compared to yearly computed riverine total N and total P load estimates.     

Mapping of accumulated nitrogen in the sediment pore water of a eutrophic lake in Iowa, USA

A large pool of nitrogen in the sediment pore fluid of a eutrophic lake in Iowa, USA, was mapped in this study. Previously, the lake had supported fishing and boating, but today it no longer supports its designated uses as a recreational water body. In the top 5 cm of the lake bottom, the pore water nitrogen ranges between 3.1 and 1,250 μg/cm³ of sediments, with an average of 160.3 μg/cm³. Vertically, nitrate concentrations were measured as 153 μg/cm³ at 0–10 cm, 162 μg/cm³ at 10–20 cm, and 32 μg/cm³ at 20–30 cm. Nitrate mass distribution was quantified as 3.67 × 10³ kg (65%) in the bottom sediments, 172 kg (3%) in suspended particulates, and 1.83 × 10³ kg (32%) in the dissolved phase. Soil runoff nutrients arrive at the lake from the heavily fertilized lands in the watershed. Upon sedimentation, a large mass of nitrogen desorbs from mineral particles to the relatively immobile pore fluid. Under favorable conditions, this nitrogen diffuses back into the water column, thereby dramatically limiting the lake’s capability to process incoming nutrients from farmlands. Consequently, a condition of oxygen deficiency disrupts the post-season biological activities in the lake.