Sequence analysis and quantitative detection of Norwalk-like viruses in cultured oysters of China

We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commer-cial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The re-suits showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient's fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×108, 1.25×108 and 4.7×101 respectively. The detecting limit of NLVs was 1×101 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future.     

广平地震台地磁异常与地震关系的研究

利用加卸载响应比、日变幅逐日比、空间相关分析法,研究分析了广平台1982-2006年地磁垂直分量观测资料与河北省及邻近地区Ms≥5.0地震之间的关系,发现它们之间有较好的对应关系;同时给出了适用于广平地磁台的地震分析预报参数,为今后广平台依磁报震提供了依据.     

日本(虫寻)和底栖短桨蟹线粒体DNA 12SrRNA基因序列的初步研究

以相应引物对日本和底栖短桨蟹的线粒体 DNA1 2 S r RNA基因片段进行了 PCR扩增和序列测定 ,分析比较了 2种间序列差异。结果表明 :日本 1 2 S r RNA基因片段长度为 41 4 bp,底栖短桨蟹为 41 5bp,2种的 A,T,G,C含量分别为 1 51 bp(36.47% ) ,1 53bp(36.96% ) ,43bp(1 0 .39% ) ,67bp(1 6.1 8% )和 1 50 bp(36.1 4 % ) ,1 55bp(37.35% ) ,44bp(1 0 .60 % ) ,66bp(1 5.90 % )。 2种间共出现了 3个碱基的缺失 /插入和 38bp的序列差异 ,其碱基转换与碱基颠换比约为 2 .1 7。     

Countermeasures for breakwater foundation subjected to foreshocks and main shock of earthquake loading

Coastal protective structures, such as composite breakwaters, are generally vulnerable to earthquake. It was observed that breakwaters damage mainly due to failure of their foundations. However, the seismically induced failure process of breakwater foundation has not been well understood. This study describes failure mechanism of breakwater foundation as well as a newly developed reinforcing model for breakwater foundation that can render resiliency to breakwater against earthquake-related disasters. Steel sheet piles and gabions were used as reinforcing materials for foundation. The experimental program consisted of a series of shaking table tests for conventional and reinforced foundation of breakwater. Numerical analyses were conducted using finite difference method, and it was observed that the numerical models were capable to elucidate the seismic behavior of soil–reinforcement–breakwater system. This paper presents an overview of the results of experimental and numerical studies of the seismic response of breakwater foundation. Overall, the results of these studies show the effectiveness of the reinforced foundation in mitigating the earthquake-induced damage to the breakwater. Moreover, numerical simulation was used for parametric study to determine the effect of different embedment depths of sheet piles on the performance of breakwater foundation subjected to seismic loading.     

武汉主城区液化潜能分区研究

搜集并整理武汉主城区地震安全性评价控制性钻孔SPT-N资料,配合武汉市地震动参数小区划成果,采用规范法对各场地土层在罕遇地震作用下液化可能性进行判别。以液化指数作为分区指标并结合各场地坐标,绘制武汉主城区液化潜能分区图。分区图初步预测了罕遇地震发生以后,可能发生液化的区域及其严重性,且表明无论是分布面积还是液化潜能,长江右岸均大于长江左岸。通过与4次历史大震液化资料的对比分析,武汉主城区可能液化场地具有地下水位埋深浅、液化土层分布范围广、埋藏深度大的特点。     

地震研究新方法——洋壳流理论简介

根据交换平衡力学原理[1],简要地介绍了洋壳流的概念及其运动特点。根据运行区域和方向的不同,把三大洋的海底扩张运动划分为不同的洋壳流,并绘出运行路线图。提出洋壳流运动方向、路线及力作用大小的判断方法,通过不同区域洋壳流相互力作用的对比,分析海底扩张运动对地震时空分布及变化规律的影响。     

印尼8.7、8.5级远震对云南地区小震的激发和地下流体异常响应初探

2004年12月26日和2005年3月29日,印度尼西亚苏门答腊西北海域相继发生8.7和8.5级地震。通过对这两次地震与云南地震活动关系和地下流体记录异常变化的分析,认为:印尼两次8级地震的发生可能会使云南提前进入新一轮强震活跃期;远震对云南地区小震的激发和导致地下流体记录出现异常变化的因素虽然都与大地震面波的强弱、地质构造环境等有关,但机理不同。     

基于性能的地震工程研究的新进展及对结构非线性分析的要求

结合国际地震工程界提出的新一代基于性能的地震工程的框架方法,重点阐述了性能评估中涉及的主要问题。对性能评估使用的静力非线性分析、动力非线性分析方法进行了总结,在此基础上详细阐述了在基于概率的性能评估中有应用前景的增量动力分析方法的概念、相关问题及其应用,并简要介绍了基于增量动力分析思想提出的一些简化方法。最后提出了今后研究的建议,特别是结构非线性分析方面的研究重点。     

海底光缆的地震影响分析

根据我国地震台网测定,北京时间2006年12月26日20时26分和34分,在南海海域发生7.2、6.7级地震。受强烈地震影响,多条国际海底通信光缆中断,造成附近国家和地区的国际和地区性通信受到严重影响。这次地震以前,海底光缆故障主要是由渔船造成,而地震的影响常常被忽视。本文在总结海底光缆3次震害经验的基础上,提出了地震影响海底光缆的主要因素以及海底光缆的抗震措施。     

塔里木地区石炭纪沉积序列与海平面变化

通过对塔里木盆地石炭系地面和钻井剖面的研究,总结出４种典型环境下的岩相特征和沉积序列。盆地内不同地区因局部沉积条件（如古水深、陆源碎屑供给量、古气候等）的不同,出现不同的沉积序列。在塔中、塔北一带多出现以碎屑岩为主的沉积序列,而塔西南多出现以碳酸盐为主的沉积序列。尽管各地区喾序列在垂向上的组合方式不同,但各沉积序列特征均能反映出海进一步退的旋回变化。结合生物地层资料,可以识别出４个三级级别（相当于