The effect of membrane filtration on estimates of microbial ATP in New Zealand freshwaters

Concentrations of microbial ATP in 15 New Zealand lakes were determined using 2 extraction techniques. ATP was found in both the paniculate (>0.45 μm) and dissolved (<0.45 μm) fractions of the water samples. Higher concentrations of paniculate ATP were obtained when ATP was extracted directly from freshwater samples rather than following pre‐concentration on to membrane filters. Recovery of particulate ATP declined with increasing volumes of sample filtered. The magnitude of the filtration effect varied unpredictably between lakes and within lakes with time. Corrections for the presence of dissolved ATP were necessary before estimates of particulate ATP could be made by the direct extraction technique. Concentrations of dissolved ATP up to 1.2 μg L^‐1 were observed. The direct extraction technique is recommended for more accurate determination of particulate ATP in New Zealand freshwaters.     

某核电厂附近海域生态环境特征及潜在致灾生物研究

为了了解某核电厂周边海域的生态环境状况,筛选潜在威胁核电厂冷源安全的致灾生物,分析影响致灾生物时空分布的主要环境因素,本研究于2018年进行了4个季节的生态调查。结果表明,研究海域浮游植物密度秋季最高,春季优势种为东海原甲藻(Prorocentrumdonghaiense),其他季节优势种为中肋骨条藻(Skeletonema costatum)等硅藻。浮游动物密度夏季最高,以桡足类为主,春、夏季优势种还包括球型侧腕水母(Pleurobrachiaglobosa)和薮枝螅水母(Obeliasp.)。底栖动物的密度和生物量在秋季最高,优势种主要为豆形短眼蟹(Xenophthalmus pinnotheroides)、长吻沙蚕(Glycera chirori)和丝异须虫(Heteromastusfiliforms)。浮游植物、浮游动物和底栖动物群落均在夏季最为稳定,群落多样性水平和物种丰度较高,且分布较为均匀。浮游植物群落的细胞丰度与悬浮物和磷酸盐的浓度正相关性最高。浮游动物的密度受温度和盐度的影响较大。底栖动物的群落分布主要受悬浮物和无机营养盐的浓度影响。本研究共筛选出17种威胁该核电厂冷...     

基于稳定同位素方法的吕泗渔场近岸海域主要生物潜在碳源及营养级分析

基于稳定同位素方法,对2018年吕泗渔场近岸海域夏季主要生物种类的潜在碳源及其营养级进行分析,利用IsoSource模型计算该海域消费者的3大碳源（浮游植物、悬浮颗粒有机物、底质有机物）贡献值,并比较叠加潜在碳源影响前后主要生物种类的营养级变化。结果表明,浮游植物和底质有机物对37种主要生物种类的贡献比例分别为58.7%和28.2%,而悬浮颗粒有机物的贡献较小。因此判定吕泗渔场近岸海域夏季主要生物种类的潜在碳源主要是浮游植物和底质有机物。通过分别叠加3大潜在碳源和单一物种作为基准值构建两个营养级谱,右营养级谱的生物种类营养级范围为1.74～3.92,比左营养级谱平均下降0.19个营养级,尽管两个营养级谱的整体趋势走向不变,但部分鱼类和多数虾类的营养位置发生改变。右营养级谱的基准值随着潜在碳源叠加比例的不同而变化,能有效反映生物营养级,更适用于低营养级的虾蟹类。但对于处于中高级消费者位置的鱼类来说,这种叠加的效果影响不大。     

半潜式超大浮体连接器动力特性的一种时间序列分析方法

根据刚性模块弹性连接器 (RMFC)假设研究由 3个模块连接而成的半潜式超大浮体MobileOffshoreBase(MOB)的波浪载荷动力响应。模块和连接器动力响应根据时间序列法计算 ,这种方法包括海况模拟 ,波浪载荷计算和结构响应计算三个主要部分。通过对结构的适当简化 ,根据Airy波理论 ,Morrison公式以及流体中的结构运动方程、弹性连接器变形方程 ,计算求得MOB在不规则海况下的动力响应 ,并将其与相关文献中的结果进行比较 ,表明该方法有良好的精确性     

Differential Colonization of Roots of Rhizophora mangle by the Wood Boring Isopod Sphaeroma terebrans as a Mechanism to Increase Root Density

Abstract. The frequency of parasitism of free ending prop roots of the red mangrove, Rhizophora mangle, by the wood boring isopod Sphaeroma terebrans, increased with increasing distance of the endings to established roots. In a field experiment, in which isolated endings were, surrounded by wooden sticks, the endings were colonized by S. terebrans at a lower rate than control endings, thus indicating that the wooden structures in the vicinity of the ending tended to reduce colonization rate by S. terebrans. It is concluded that S. terebrans may cause mangrove root density to increase by colonizing peripheral endings more readily than endings which are near established roots.     

Impact of urban stormwater runoff on estuarine environmental quality

Urban stormwater runoff could contribute to the deterioration of water quality of a receiving water body. In this research, field studies and laboratory experiments were conducted to assess the microbial contamination resulting from urban stormwater runoff into the Lake Pontchartrain estuary. Fecal coliform, Escherichia coli and enterococci were used as indicator organisms. The specific objectives of the research were to examine the distribution of the indicator organisms in different environmental elements (water column, suspended particles and sediment) and to further investigate the mechanisms related to their fate. Results of the research indicated satisfactory water quality at the study sites during dry weather periods. However, a significant increase was observed in the concentrations of the indicator organisms in the water columns and sediment at specific study sites following a given stormwater event. Three to seven days were needed for the elevated indicator organisms to return back to their background levels in the water column and sediment, respectively. The mechanism of sedimentation contributed to a reduction in the microbial concentration in the water column, as the indicator organisms were found to attach to the suspended particles in the stormwater. The percentage of fecal coliform, E. coli and enterococci attached to the suspended particles was found to be within the range of 9.8–27.5%, 21.8–30.4%, and 8.4–11.5% of the total indicator organisms in the stormwater loaded into the estuary, respectively. About 75–80% of the total indicator organisms remained free-floating for some distance in the water column before dying off.     

鲈鱼胆汁中芘及代谢产物含量与水体中芘含量的关系

运用GC—MS的方法和荧光分光光度法，研究鲈鱼分别暴露于0．1、1．0和10．0μg／dm^3质量浓度芘溶液中7d，水体中芘及其鲈鱼胆汁中芘和1-羟基芘含量的变化,实验结果显示：(1)鲈鱼对水体中的芘具有非常显著的去除作用。(2)随着芘暴露浓度的增大，鲈鱼对芘的代谢去除作用增强。(3)随着暴露时间的延长和芘暴露浓度的增大，胆汁的1-羟基芘浓度递增。(4)鲈鱼胆汁具有较高的芘浓度，对海水中的芘具有较强的富集作用。(5)鲈鱼胆汁的1-羟基芘及芘的浓度与水体芘浓度均具有很好的相关性，对于指示水体的芘污染程度具有一致性；可作为指示水体芘污染程度的生物标志物。     

Numerical prediction of MODUs drift during Hurricane Katrina

Severe hurricanes, such as Katrina, broke the mooring lines of a number of mobile offshore drilling units (MODU) deployed in the Gulf of Mexico and some of those MODUs went adrift. A drifting MODU may damage other critical elements of the offshore oil and gas infrastructure by colliding with floating or fixed production systems and transportation hubs, or by rupturing pipelines owing to their dragging anchors over the seabed. To avoid or mitigate the damage caused by a drifting MODU, it is desirable to understand the mechanics of the drift of a MODU under the impact of severe wind, wave and current and have the capability of predicting the trajectory of the drift. To explore the feasibility and accuracy of predicting the trajectory of a drifting MODU based on hindcast met-ocean conditions and limited knowledge of the condition of the drifting MODU, this study employed a simplified equation describing only the horizontal (surge, sway and yaw) motions of a MODU under the impact of steady wind, current and wave forces. The simplified hydrodynamic model neglects the first- and second-order oscillatory wave forces, unsteady wind forces (owing to wind gustiness), wave drift damping, and the effects of the body oscillation on the steady wind and current forces. It was assumed that the net effects of the oscillatory forces on the steady motion are insignificant. To verify the accuracy and feasibility of our simplified approach, the predicted drifting trajectories of two MODUs were compared with the corresponding measurements recorded by the global positioning system (GPS).     

泉州湾赤潮藻类优势种细胞密度回归方程研究

于2006年5-11月对东海泉州湾赤潮监控区四个监测站位开展赤潮常规监测。根据监测结果,分别以各站位23项水质理化生物环境因子指标为自变量,相应赤潮藻类优势种的细胞密度为因变量,进行多元逐步回归分析,建立了各站位优势种中肋骨条藻(Skeletonema costatum)、太平洋海链藻(Thalassiosira pacifica)、微小原甲藻(Prorocentrum minimum)、尖刺拟菱形藻(Pseudo-nitzschia pungens)、丹麦细柱藻(Leptocylindrus danicus)和旋链角毛藻(Chaetoceros curvisetus)等的细胞密度多参量回归方程。结果表明,所有回归方程的复相关系数都接近于1,方差分析的结果均为回归极显著,表明所建立的回归方程可作为相应赤潮优势种细胞密度预报方程的高度有效性,将对今后泉州湾的赤潮预报提供良好的指导作用。