Diagnosis of iridovirus in large yellow croaker by PCR

A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10^-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection.     

无公害刺参稳产健康养殖新技术

本文针对目前刺参养殖中存在的问题,简单明了叙述刺参无公害健康养殖一系列新技术和新方法,对推动海参养殖业持续、稳定、健康发展,起到积极作用和效果。     

基于EMD与神经网络的机械故障诊断技术

经验模式分解 (EMD)是分析非线性、非平稳信号的有力工具 ,它将信号分解为突出了原信号的不同时间尺度的局部特征信息的内在模函数 (IMF)分量。本文通过将各 IMF分量输入到 BP网络中进行训练学习和故障诊断 ,比直接输入原信号可以提高 BP网络对故障诊断的准确率 ,而且减少了训练时间。     

鲈鱼胆汁中芘及代谢产物含量与水体中芘含量的关系

运用GC—MS的方法和荧光分光光度法，研究鲈鱼分别暴露于0．1、1．0和10．0μg／dm^3质量浓度芘溶液中7d，水体中芘及其鲈鱼胆汁中芘和1-羟基芘含量的变化,实验结果显示：(1)鲈鱼对水体中的芘具有非常显著的去除作用。(2)随着芘暴露浓度的增大，鲈鱼对芘的代谢去除作用增强。(3)随着暴露时间的延长和芘暴露浓度的增大，胆汁的1-羟基芘浓度递增。(4)鲈鱼胆汁具有较高的芘浓度，对海水中的芘具有较强的富集作用。(5)鲈鱼胆汁的1-羟基芘及芘的浓度与水体芘浓度均具有很好的相关性，对于指示水体的芘污染程度具有一致性；可作为指示水体芘污染程度的生物标志物。     

塘沽海区海底地形的SAR影像仿真与反演研究

利用袁业立、金梅兵(1997)提出的海底地形SAR影像仿真与反演模型，对渤海塘沽海区的一张Radarsat SAR影像进行了仿真和水深反演研究。研究结果表明：仿真影像与真实SAR影像基本吻合，反演水深与实际水深也有较好的一致性；进一步证实了袁业立(1997)SAR成像机理的正确性和在中国近海利用SAR影像进行浅海水深探测的可行性。     

Two-Dimensional Mathematical Model of Tidal Current and Sediment for Oujiang Estnary and Wenzhou Bay

A 2-D mathematical model of tidal current and sediment has been developed for the Oujiang Estuary and the WenzhouBay. This model accomodates complicated features including multiple islands, existence of turbidity, and significant differ-ence in size distribution of bed material. The governing equations for non-uniform suspended load and bed load transport arepresented in a boundary-fitted orthogonal curvilinear coordinate system. The numerical solution procedures along with theirinitial conditions, boundary conditions, and movable boundary technique are presented. Strategies for computation of thecritical condition of deposition or erosion, sediment transport capacity, non-uniform bed load discharge, etc. are suggested.The model verification computation shows that, the tidal levels computed from the model are in good agreement with the fielddata at the 18 tidal gauge stations. The computed velocities and flow directions also agree well with the values measuredalong the totally 52 synchronously observed verticals distributed over 8 cross sections. The computed tidal water throughputsthrough the Huangda‘ao cross section are close to the measured data. And the computed values of bed deformation fromYangfushan to the estuary outfall and in the outer-sea area are in good agreement with the data observed from 1986 to 1992.The changes of tidal volumes through the estuary, velocities in different channels and the bed form due to the influence of thereclamation project on the Wenzhou shoal are predicted by means of this model.     

Simple determination of dimethylsulfide (DMS) and dimethylsulfoniopropionate (DMSP) using solid-phase microextraction and gas chromatography-mass spectrometry

Solid-phase microextraction (SPME) is a simple, sensitive and less destructive method for the determination of dimethylsulfide (DMS) in seawater. Combined with detection by gas chromatography-mass spectrometry (GC-MS), the method had sufficient sensitivity (minimum detectable concentration of DMS was 0.05 nM), and practical levels of reproducibility (relative standard deviation ≤7%) and linearity (r ² > 0.995) over a wide concentration range (0.5 to 910 nM). The protocol developed was applied to a Sagami Bay water sample to determine concentrations of DMS and DMSP, and in situ DMSP-lyase activity.     

陀螺经纬仪的读数方法比较研究

介绍了陀螺定向测量中三种不同的数据采集方法，分析了各种方法的优缺点。作者认为传感器采集数据自动化程度高，精度高，特别是运用位置敏感探测器（PSD）采样数据量大，具有一定的抗干扰能力，但在运用传感器采样时必须注意克服非线性误差。