Screening of marine fungus from Nanji Island and activity of their metabolites against pathogenic Vibrio from Pseudosciaena crocea

Seventy-eight marine fungal strains were isolated from sediment samples collected off the coast of Nanji Island,Wenzhou,Zhejiang Province,China.Antibacterial screening using the agar disc method showed that 19 of the isolated strains could inhibit at least one pathogenic Vibrio from Pseudosciaena crocea.Subsequent screening confirmed that nine strains produced antibacterial metabolites that had activity against one or several types of pathogenic Vibrio.Strain NJ0104 had the widest antimicrobial spectrum and strong activity,particularly against Vibrio parahaemolyticus-MM0810072.A preliminary study of NJ0104 antibacterial metabolites demonstrated that they had thermal stability up to 80°C,ultraviolet stability up to 40 min and pH stability between 4.0-7.0.In addition,the antibacterial metabolites were readily soluble in butanol.To identify the specific strain,the ITS-5.8S rDNA regions of NJ0104 were PCR amplified and sequenced.Based on the combination of phenotypic and genotypic data,the strain was identified as Arthrinium sp.     

尼泊尔Ms8.1地震构造背景及对周边地区地震活动趋势的影响

分析印度板块与欧亚板块俯冲带、尼泊尔Ms8.1地震震中区地震构造及地震发震构造,讨论主震对余震触发及余震的时空强特征和地震对周边地区地震活动趋势的影响。初步分析认为,主边界断裂为本次地震的发震构造,属于低角度逆冲断层地震;余震分布范围与震源破裂面积和方式基本一致,具有向东迁移的时空特征;西藏定日Ms5.9、聂拉木Ms5.3地震不属于尼泊尔地震余震,是应力扰动的结果;沿俯冲带向东至喜马拉雅东构造结是大震发生的危险地段。     

蒙古中部剖面重力异常研究

在蒙古中部106°E附近布设一条南北走向的重力剖面,首次获得该地区精确的实测重力值,经过数据预处理和改正计算,得到剖面重力异常结果。分析表明,剖面自由空气异常和地形有很强的相关性|剖面布格重力异常变化总体较为平缓,只在戈壁-阿尔泰-曼达尔戈壁加里东区带(GAB)和哈拉加里东区带（HRB）附近发生相对较快的变化,推测这两个区域可能存在较明显的深部构造变化|在蒙古-鄂霍兹克造山带附近,并未发现与板块缝合带相对应的重力异常变化|剖面均衡重力异常以负值为主,多数地区地壳未达到重力均衡状态,剖面附近地震活动和均衡重力异常之间的关系与中国南北地震带北段相关研究结果类似。     

晋冀蒙地区的垂直形变特征

基于1998、2006和2013年3期水准复测资料,平差计算得到1998~2006年和2006~2013年的垂直形变速率及其垂直形变速率梯度。根据计算结果研究晋冀蒙地区的地壳垂直形变运动趋势以及主要断裂带的活动性,分析区域垂直形变速率和垂直形变速率梯度的演变。结果揭示出吕梁山地块后一时段上升速率增加、太行山地块由前一时段上升变为后一时段下降、盆地地带变化复杂的垂直形变态势。     

德干断裂系的特征、活动性与地震危险性评价

乌东德水电站场址附近走向NNE的德干断裂系主要由裸佐断裂、马店断裂、鹿鹤断裂等组成,全长约120 km。该断裂系向北收敛,向南呈“帚状”撒开成多条分支近平行排列,展布宽约6 km。断层构造岩均为脆性变形所产生的碎裂岩系,断裂带内各种次级组构表明,该断裂系在新构造时期存在多次构造活动,且活动强度由强逐渐变弱。断裂最新活动年代在中更新世,运动方式为粘滑兼有稳滑。地震危险性分析认为,倮佐断裂的最大地震震级为6.0级,马店断裂、鹿鹤断裂等在5～5.5级之间。     

基于水准剖面的山西断陷带现今活动性研究

根据跨山西断陷带的水准剖面观测资料,计算其垂直形变量和垂直形变速率,研究其现今垂直活动性。2006年以来的垂直形变揭示,山西断陷带东北段延怀盆地和蔚县盆地呈掀斜上升活动,北段阳原盆地和大同盆地以下降活动为主,晋中盆地为上下波动,临汾盆地呈现上升活动。2013年山西断陷带活动出现转折性变化,蔚县盆地大幅度下降,大同盆地、代县-定襄盆地、晋中盆地和临汾盆地均呈现不同程度的上升。     

拮抗香蕉枯萎病菌海洋细菌的筛选和鉴定

从93份中国南部海域近海海泥样品中分离出1800多株海洋细菌,以对峙培养法,测定海洋细菌对香蕉枯萎菌的拮抗作用,再以琼脂划线培养法(agar streak)测定菌株的抑菌活性,结果表明:约55.6%的菌株对香蕉枯萎菌有一定的拮抗作用,其中58株菌株抑制香蕉枯萎菌生长所形成的抑菌带宽度大于10.0mm,抑菌作用较好,复筛后其中13个菌株形成的抑菌带宽在15.0mm以上。经反复转接和对峙培养,筛选出拮抗效果好、抑菌活性稳定的菌株TC-1,初步鉴定为芽孢杆菌属的细菌,TC-1对14种病原微生物都有较好的抑菌效果。     

The scavenging of free radical and oxygen species activities and hydration capacity of collagen hydrolysates from walleye pollock (Theragra chalcogramma) skin

Fish skin collagen hydrolysates (FSCH) were prepared from walleye pollock (Theragra chalcogramma) using a mixture of enzymes, namely trypsin and flavourzyme. The degree of hydrolysis of the skin collagen was 27.3%. FSCH was mainly composed of low-molecular-weight peptides and the relative proportion of <1000Da fraction was 70.6%. Free radical and oxygen species scavenging activities of FSCH were investigated in four model systems, including diphenylpicrylhy-drazyl radical (DPPH), superoxide anion radical, hydroxyl radical and hydrogen peroxide model, and compared with that of a native antioxidant, reduced glutathione (GSH). FSCH was also evaluated by water-absorbing and water-holding capacity. The results showed that FSCH was able to scavenge free radical and oxygen species significantly and to enhance water-absorbing and water-holding capacity remarkably. Therefore, FSCH may have potential applications in the medicine and food industries.     

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Immunotoxicity effect of benzo[α]pyrene on scallop Chlamys farreri

The toxic effects of benzo[α]pyrene (B[α]P) at different concentrations (0.1, 0.5, 1, 2.5 and 7.5 μgL⁻¹) on scallop (Chlamy farreri) immune system were studied. The results showed that B[α]P had significant toxic effects on the haemocyte counts, neutral red uptake, phagocytosis, bacteriolytic and antibacterial activity (P<0.05), while the seawater control and acetone control had no significant differences. The haemocyte counts, neutral red uptake, phagocytosis and bacteriolytic activity in all B[α]P treatment groups as well as antibacterial activity in groups of 0.5, 1, 2.5 and 7.5 μgL⁻¹ B[α]P decreased significantly (P<0.05). Some of these indices tended to be stable on the sixth day and others on the ninth day, and the indices showed clear time- and concentration-response to B[α]P. Bacteriolytic activity in 0.1μgL⁻¹ B[α]P treatment group and antibacterial activity in 0.1 μgL⁻¹ and 0.5 μgL- B[α]P treatment groups increased at the beginning of exposure and reached their peaks on day 1 and day 6, respectively. Following that, both activities decreased gradually and became stable after day 9. When all the indices reached stability, they were significantly lower than those in control group (P<0.05), except for antibacterial activity in 0.1 μgL⁻¹ B[α]P treatment group (P>0.05). Thus, B[α] has evident toxic effects on scallop immune system, which supports the view that a relationship exists between pollution and immunomodulation in aquatic organisms.