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High-performance thin-layer chromatography with automated multiple development (AMD-HPTLC) was applied for the examination of pulp mill bleachery effluents from an alkali-oxygen-peroxide (EOP) bleaching stage of a sulfite pulp. A gradient based on methanol and dichloro-methane was developed for the separation of the effluent compounds over a wide range of migration distances. A series of reference substances such as lignin phenols and other aromatic acids, aldehydes, and alcohols as well as two resin acids were investigated in parallel using the AMD-HPTLC method. Characterization of the effluent compounds was possible using the migration distances and the recorded spectra. The composition of different samples of effluent were found to be very similar. The application of the AMD-HPTLC to effluent fractions from acidic liquid-liquid extraction using diethyl ether and from RP C18 solid-phase extraction allowed us to follow the behaviour of the effluent compounds in the extraction procedure. 相似文献