凡纳滨对虾TOR蛋白下游信号传导因子eIF4E2和eIF4E1A基因克隆与功能分析

为研究mTOR信号通路在凡纳滨对虾(Litopenaeus vannamei)氨基酸代谢中的调控作用,本研究利用RACE技术首次克隆获得了凡纳滨对虾肌肉组织中eif4e2和eif4e1a基因的全长c DNA序列。eif4e2基因c DNA序列全长1 069 bp,开放阅读框699 bp,编码232个氨基酸;eif4e1a基因c DNA序列全长3579bp,开放阅读框627bp,编码208个氨基酸。氨基酸序列比对和进化分析均证实eIF4E2和eIF4E1A为目前已知的eIF4E家族蛋白的同源蛋白。利用实时荧光定量PCR的方法研究了eif4e2和eif4e1a基因在凡纳滨对虾不同组织中的表达差异以及注射雷帕霉素或氨基酸后肌肉中eif4e2和eif4e1a基因表达量的变化情况,探讨了eif4e2和eif4e1a基因在细胞生长中的调控作用。结果表明,凡纳滨对虾eif4e2和eif4e1a基因在眼柄、肝胰脏、肠道、胃、鳃丝和肌肉等组织中均有表达;其中eif4e2基因在肌肉中的表达量显著高于其他组织(P0.05),eif4e1a基因在肠道和肌肉中都有较高表达量,且在肠道中的表达量显著高于其在肌肉中的表达量(P0.05);注射雷帕霉素后2 h内肌肉中eif4e2和eif4e1a基因表达量都出现显著下降(P0.05);肌肉中eif4e1a基因的表达量在单独注射亮氨酸或精氨酸4 h内均未出现变化,但在同时注射亮氨酸和精氨酸后表达量显著增加(P0.05);肌肉中eif4e2基因在注射亮氨酸、精氨酸及亮氨酸加精氨酸后表达量都明显提高(P0.05),且同时注射亮氨酸和精氨酸后,基因表达变量明显比单独注射亮氨酸或精氨酸后变化量大。本研究从动物分子营养学角度出发,克隆了凡纳滨对虾mTOR信号通路中eif4e2和eif4e1a两个基因,并证明其能够通过mTOR信号通路接收不同氨基酸信号来调控细胞生长,对于深入了解对虾的生长和代谢调控机制、饲料配方的优化以及建立合理的养殖管理模式都具有重要的意义。     

个旧东区东西向断裂控岩控矿特征

对东西向构造控岩控矿特征进行全面总结分析,讨论了东西向断裂深部找矿的意义,指出深部找矿方向.     

Modification of the polyethylene glycol 6000 precipitation method for recovering human and indicator viruses from oysters and mussels

Human viruses are a common contaminant of shellfish affected by human sewage wastes. They are difficult to detect as they are not easily separated from shellfish tissue. This paper describes a modification of the polyethylene glycol (PEG) precipitation technique for recovery of enteroviruses and F‐specific bacteriophages from the Pacific oyster (Crassostrea gigas) and the green‐lipped mussel (Perna canaliculus). Modifications adopted were the use of only the digestive gland tissue for virus extraction, resuspension of the primary PEG pellet in 4 volumes of eluent, and the introduction of a secondary PEG precipitation to reconcentrate the virus containing extract. The recovery rate of the virus extraction process was not affected by introduction of the secondary concentration step (overall recovery remained at 60–70% of the virus input). The advantages of reduction of tissue residue in the extract, smaller final volume, and the ability to process 2–3 times the number of individual shellfish for the same effort, improve the practicality of the method.     

Rhythmic swimming of the isopod Exosphaeroma obtusum (Dana)

The tidal swimming rhythm of the New Zealand rocky shore isopod Exosphaeroma obtusum (Dana) is described. Peak swimming was on the ebb tide about 2.5 h after high water; the peak in the dark period was 6–8 times greater in amplitude than the daytime peak. Positive thigmotaxis was shown at all other times. The rhythm period in continuous darkness (free‐running period) was greater than tidal periodicity, and there was a semi‐lunar swimming pattern with peaks at the first and third quarters of the moon. Rheotropic experiments showed that changes in water flow did not initiate the swimming rhythm. The work was compared with the infaunal species Pseudaega punctala and the northern Eurydice pulchra. It was concluded that infaunal species rely more on endogenous control of the swimming rhythm because of their isolation, when buried, from exogenous Zeitgeber.     

Construction of white spot syndrome virus (WSSV) whole genome phage display library

A rebuilt vector pCANTAB 5 EE was obtained by inserting a 34 bp double-stranded oligonucleotide which contained a EcoRV recognition sequence into pCANTAB 5 E. White spot syndrome virus (WSSV) genome DNA was fragmented by sonication to isolate fragments mainly in the range of 0.8~2.0 kb, then the fragments were blunt-ended with T4 DNA polymerase and cloned into the EcoRV site of pCANTAB 5 EE. The primary recombinant clone of the library was 3.0×105. Colony PCR of random selected recombinants showed that the size of the inserts was 0.12~1.77 kb. After the whole library recombinant phages infected Escherichia coli HB2151 cells, the extracellular and periplasmic extracts were dropped on PVDF membranes to perform dot blot, using polyclonal mouse anti-VP24 serum, anti-WSV026 serum, anti-WSV063 serum, anti-WSV069 serum, anti-WSV112 serum, anti-WSV238 serum, anti-WSV303 serum and anti-VP26 serum as the primary antibody, respectively. The results showed that the display library could express the viral proteins.     

青藏高原羌塘盆地东部鄂尔陇巴组火山岩的锆石SHRIMP U—Pb年龄及其地质意义

藏北羌塘盆地东部地区三叠系巴贡组之上沉积超覆了一套火山岩-火山碎屑岩．该套地层下部玄武岩的锆石SHRIMPu—Pb年龄明显可以分为2组,一组年龄相对年轻（220．4Ma±2．3Ma）,代表了羌塘东部地区鄂尔陇巴组火山岩的喷发年龄;另外一组年龄相对较老（241．4Ma±3．6Ma）,可能与中三叠世羌塘地区普遍存在的碰撞隆升事件有关。羌塘中生代（晚三叠世-早白垩世）盆地演化早期的沉积作用经历了由陆相至海相的超覆过程,沉积超覆作用从冲洪积相开始,伴随着岩浆侵入、火山爆发及火山碎屑沉积作用,总体上表现为一个向上变深的海侵序列,显示裂谷盆地的特征。     

养殖锯缘青蟹呼肠孤样病毒粒子的电镜观察

经过对患病青蟹Scylla serrata的组织进行了超薄切片电镜观察,在病蟹的鳃上皮细胞、心肌细胞、胃和肠道上皮细胞中发现了大量的呼肠孤样病毒粒子。该病毒粒子的大小为60 nm左右,二十面体对称,无囊膜,在感染组织细胞浆中呈晶格状排列。根据病毒的形态特征和组织分布形态,初步认为该病毒为水生呼肠孤病毒,暂将其命名为青蟹呼肠孤病毒(Scylla serratareo-like virus,Ss-REO)。     

El Nino对云南初夏降水的影响

利用云南初夏５－６月的降水资料，全球月平均海温资料及热带ＯＬＲ资料，研究了云南初夏５－６月降水对海气相互作用特别是Ｅｌ Ｎｉｎｏ的响应，发现云南初夏降水与赤道东太平洋海温变化以及南海对流活动有密切关系。     

Study On Development And Application Of A Regional Pbl Numerical Model

A regional scale (a few km to a few hundred km, minutes to days)planetary boundary layer (PBL) numerical model (RPBLM) has been successfully developed to simulateprecisely the boundary-layer three-dimensional characteristics. The RPBLM model with a high-resolutionnon-hydrostatic E - turbulence closure scheme has been applied to diagnose and prognosticate the PBL characteristicsover a complex underlying surface in the Hong Kong area of 60 km × 48 km. It is shown that the RPBLMmodel can be used to simulate the PBL characteristics including wind, temperature, water vapour andturbulence over such a complex underlying surface and that the simulated result is reasonably in agreementwith observations.