Development of a fluorescence quenching assay to measure the fraction of organic carbon present in self-assembled gels in seawater

The dissolved organic carbon pool (DOC) is among the largest reservoir of reduced carbon on our planet. The demonstration that DOC polymers remain in assembly/dispersion equilibrium forming microscopic hydrogels has a broad range of critical implications. Previous studies estimate that  10% of DOC could be assembled as gels, yielding values of  7 × 10¹⁶ g of organic carbon present as microscopic hotspots of high substrate concentration. This huge mass of reduced carbon emphasizes the need to develop reliable methods to systematically investigate the budget of self-assembled marine gels (SAG), and their role in biogeochemical cycling. However, a quantitative method to measure SAG in seawater has not been available. Here we present the validation of a simple assay to measure the mass of organic carbon assembled as microgels in native seawater. This method is based on the ratio of Chlortetracycline (CTC) fluorescence quenching between Ca bound to non-assembled organic molecules and molecules assembled as microgels. This assay can be readily implemented on board using a low cost fluorometer and provisions to measure TOC.     

巴基斯坦N区块浪控三角洲沉积相划分及模式

浪控三角洲砂体是海陆交互带沉积有利的油气储层和勘探目标,目前业界对其微相划分、沉积机理和沉积模式研究较少.以巴基斯坦N区块为例,依据岩心、测井、地震及分析测试资料,开展受波浪改造作用的海相三角洲沉积相带划分、展布及沉积模式研究.结果表明:综合大量沉积相标志分析,研究区白垩系处于砂质高能海岸带,三角洲入海口受到强烈的波浪...     

Anthropogenic marker evidence for accelerated sedimentation in Lake Illawarra,New South Wales,Australia

The chronology of near-surface sediments in Lake Illawarra has been investigated using radiocarbon dating and anthropogenically derived substances including trace metals, ash, and¹³⁷Cs. Sediments at depths about 1 m below the water-sediment interface ranged in age from Modern to 786 calendar years bp on the basis of radiocarbon dating ofNotospisula trigonella valves. Multiple marker (for example ash-trace metals) depth-concentration sediment profiles yielded estimates of sedimentation rate ranging from 3 to 5 mm yr^–1 at Griffins Bay to more than 16 mm yr^–1 at Macquarie Rivulet. Sedimentation rates of approximately 10 mm yr^–1 appeared to be typical of the western and southwestern portions of the lagoon. Rates of sediment accretion, prior to catchment clearing, urbanization, and industrialization have been estimated at less than 1 mm yr^–1, thus indicating a general tenfold increase in sediment accumulation adjacent to the western foreshore caused by catchment development. Accelerated sedimentation in shallow coastal lagoons constitutes significant environmental impacts including shoaling, degradation of seagrass beds, and increased turbidity with consequent loss of aesthetic appeal. Management policy should be directed at attempts to reduce the amount of sediment input by the construction of strategically placed sediment retention ponds. Siltation within the shallow embayments of Lake Illawarra could be ameliorated by a carefully planned program of dredging.     

基于MySQL的测量标志点可视化管理与导航寻点系统

设计开发了基于MySQL的测量标志点可视化管理及导航寻点系统,给出了系统总体构架和服务端与移动端的数据交互方式,阐述了系统中测量标志点可视化和信息化管理、移动端导航寻点和信息采集、导航轨迹测量与简化、多控制点的导航寻找等关键问题的解决方案。系统运行表明,服务端地图显示测量标志点和查询结果,界面直观、友好,实现了测量标志点可视化信息化管理;移动端的导航寻点功能可以提高外业作业效率。     

Alkylbenzenes in mussels from South and South East Asian coasts as a molecular tool to assess sewage impact

Alkylbenzenes, molecular markers of sewage, were measured in 34 green mussels collected from India, Indonesia, Malaysia, Thailand, Cambodia, Vietnam, and the Philippines together with blue mussels collected from Tokyo Bay, Japan. Linear alkylbenzene (LAB) concentrations in South and South East Asian countries ranged from 10 to 1640 ng-∑LAB/g-dry tissue. In some populous cities, LAB concentrations were similar or higher than those found in northern Tokyo Bay which is heavily impacted by sewage effluents. I/E ratios (a ratio of internal to external isomers of LABs) in the South and South East Asian countries (1–3) were much lower than those in Tokyo Bay (3–8), indicating sewage discharged in the coastal zone is poorly treated (e.g., raw sewage and/or primary effluents). Alkylbenzenes with branched alkyl chains, tetrapropylene-based alkylbenzenes, were also detected in mussels from Indonesia and Philippines. This “tell-tale” sign indicates that poorly degradable detergents are still in use in this area, although they have long been phased-out in many industrialized countries.     

Biogeochemical aromatic markers in the sediments from mahakam delta (Indonesia)

Analysis of the tri-, tetraand pentaaromatic hydrocarbon fractions of several sediments (0–600 m) from the Mahakam Delta (Indonesia), indicate the predominance of polycyclic aromatic hydrocarbons (PAH) diagenetically related to triterpenoid natural precursors. High resolution Shpol'skii spectrofluorometry (HRS) proved to be a valuable analytical tool, in conjunction with high performance liquid chromatography (HPLC) and capillary gas chromatography coupled with mass spectrometry (CGC/MS), for the identification of individual components in Chromatographic fractions obtained after a high resolution reverse phase liquid chromatography.     

辽河盆地西部凹陷下第三系原油和生油岩的生物标记化合物研究

本文应用色谱-质谱和气相色谱等技术分析鉴定了辽河断陷盆地西部凹陷下第三系原油和生油岩饱和烃中的甾、萜烷等生物标记化合物。并利用甾、萜烷等生物标记化合物和其它地质、地球化学资料,讨论了油、岩的生物先体性质,将其做了分类对比,确定了沙河街组、东营组和前中生界潜山原油的原生性和次生性。     

基于数学形态学的IKONOS多光谱图像分割方法研究

利用数学形态学方法,研究与探讨了IKONOS多光谱图像的分割技术.提出一种结合图像边缘特征和纹理特征的混合分割新算法.在高分辨率多光谱遥感图像K-L变换的基础上,采用多尺度多方向形态学梯度算子提取边缘特征.应用数学形态学滤波及局部方差统计特征对图像对象进行标记,最后采用强制最小过程,进行标记控制的分水岭分割.研究结果表明,提出的分割算法优于仅利用边缘特征的分水岭分割算法,同时,该算法能较好地解决分割过程中存在的过分割与欠分割问题,是一种适合高分辨率多光谱遥感图像的分割算法.     

腾格里沙漠昂格尔图湖记录的988 AD以来的古气候变化

通过对腾格里沙漠东南部昂格尔图湖岩芯AGE15A的粒度、碳酸盐的百分含量和主要化学元素等多项沉积学气候代用指标及精确定年的综合分析,重建了该区域988 AD以来的古气候变化序列。结果表明：988 AD以来的研究区的气候环境的演化过程与我国西北西风区古气候变化基本一致,呈现"冷湿-暖干"交替变化的特征,但在时间上存在一定的延迟。具体表现为：988~1383 AD,昂格尔图湖由洼地发育成小型湖泊,化学风化作用弱,湖区气候环境整体偏冷湿,期间出现几次暖波动;1383~1560 AD,研究区处在中世纪暖期,为温暖、干旱的气候特征,此时湖泊扩张,湖中水生植物生长茂盛,呼吸作用增强,造成大量的碳酸盐沉淀,最高值达到8.16%;1560~1700 AD,致使气候在昂格尔图湖形成之后出现最寒冷的时期,降水增加,气候湿冷,此时段与小冰期最盛期相对应;1700 AD之后,温度开始上升,气候回暖,在1900 AD左右达到最甚,此时沉积物中碳酸盐的百分含量也达到最大值,为10.15%,此期间湖泊继续扩张,出现几次气候冷波动。基于我国古代文献重建的历史时期气候变化基本不包括沙漠地区,所以本文利用湖相沉积记录来反演腾格里沙漠昂格尔图湖历史时期的气候环境演变,对中国沙漠研究有着重要参考价值,同时也为中国西北历史时期的气候重建提供科学依据。     

翘嘴鳜(Siniperca chuatsi)EST-SSR标记与生长性状相关性及4个选育群体遗传结构研究

采用翘嘴鳜(Siniperca chuatsi)转录组高通量测序并筛选得到的13对多态性EST-SSR引物,对60尾翘嘴鳜的体长、体高、体厚及体重4种生长性状进行标记-性状相关性研究,并对4个翘嘴鳜选育群体各30尾个体进行遗传结构分析。结果显示,与其生长性状显著相关的微卫星分子标记有5个,包括:分别与体厚和体长极显著相关(P0.01)和显著相关(P0.05)的标记C10127,AA型为体长、体厚的优势基因型,而AC型为劣势基因型;与体重显著相关(P0.05)的标记C19016,AA型为体重的优势基因型,AB型为体长的劣势基因型;与体厚显著相关(P0.05)的标记C14847,BB型为体厚的优势基因型;分别与体长、体高和体重、体厚极显著相关(P0.01)和显著相关(P0.05)的标记C12350,BC型为体长、体厚、体高和体重性状的优势基因型;分别与体厚、体重和体高极显著相关(P0.01)和显著相关(P0.05)的C18691标记,BB型为体厚、体重和体高性状的优势基因型。4个翘嘴鳜群体遗传多样性高低依次为太湖选育群体长江口选育群体洪泽湖选育群体鄱阳湖选育群体,其平均的等位基因数、有效等位基因数、观测杂合度、期望杂合度和多态信息含量分别为2.2115、1.9405、0.55513、0.5925和0.3775,属于中度多态水平。