近海温泉中嗜热菌Geobacillus sp. ZH1锰超氧化物歧化酶的克隆与表达

将嗜热菌Geobacillus sp. ZH1的超氧化物歧化酶(supseroxide dismutase,SOD)基因插入表达载体pET-32α(+),在Escherichia coli BL21(DE3)中进行表达,并利用亲和层析纯化重组超氧化物歧化酶.将制备的脱辅SOD进行Mn2+和Fe2+金属重构后,得到的Mn2+重构SOD的比活力达668U/mg,Fe2+重构Fe-SOD没有活性,说明ZH1菌株的超氧化物歧化酶为Mn-SOD.凝胶过滤及SDS-PAGE分析显示,Mn2+重构SOD为同聚二聚体,亚基分子量为71.7 kDa.这些研究结果为进一步研究该酶的生化特性及酶的定向进化研究奠定了良好的基础.     

重金属胁迫对两种海洋饵料微藻的急性毒性效应研究

在模拟生态环境条件下研究了铜(Cu2+)、镉(Cd2+)和锌(Zn2+)3种重金属离子对2种海洋饵料微藻(青岛大扁藻(Platymonas helgolandica)和等鞭金藻8701(Isochrysis galbana 8701))的急性毒性效应,分析其对种群增长的影响,并利用透射电镜对微藻亚显微结构的变化进行分析。在实验浓度范围内,2种微藻的种群增长对3种重金属离子的急性胁迫表现出类似的变化趋势,随着胁迫浓度的增加,微藻的相对增长率均呈现出不断下降的趋势;通过比较2种微藻的48hEC50和96h-EC50发现:3种重金属离子的毒性程度为Cu2+Cd2+Zn2+,等鞭金藻8701对重金属胁迫的敏感性高于青岛大扁藻。2种微藻的亚显微结构观察显示,二者的膜结构及细胞核对Cu2+胁迫并不太敏感;与对照组相比等鞭金藻8701的色素体及贮能物质受影响较严重,而青岛大扁藻的叶绿体及淀粉粒受影响较大。     

海洋测量成果质量控制的探讨

结合实际测量过程中出现的问题,对提高海洋测量成果质量的几个关键环节进行了思考,从标准规范、规章制度到人员素质等三个方面提出了相应的建议,对于提高技术人员的基本素质、严格执行标准规范和规章制度、全面提高测量成果质量具有一定的参考意义。     

机载激光测深技术

首先基于海水的光学特性介绍了机载激光测深原理,然后根据国内外的研究现状介绍了机载激光测深系统的性能指标和系统构成,最后介绍了在海底地形测量、障碍物探测、近岸海洋工程建设等方面的应用。为从事机载激光测深系统研发和应用的技术人员提供相关原理和背景参考。     

Characterization of marine microplankton communities of Qingdao coastal areas using randomly amplified polymorphic DNA（RAPD）

Microplankton communities of three coastal sites of Qingdao, Shandong Province, China were investigated using RAPD (random amplified polymorphic DNA) molecular markers and morphological observations. Eight RAPD-primers were selected to amplify the DNA polymorphy. The genetic distances inferred from the pairwise similari-ties were calculated for the phylogenetic tree construction. Meantime, the traditional microscopic determination, a way of visualizing the species composition, was performed to detect the major taxa of microplanktons from all samples. Results showed that: (1) the band sharing index values were in the range of 0. 504 2-0. 763 2 among samples from the same sampling site at different time scales, while 0.406 5-0.685 7 among the samples from different stations at the same time scales, indicating that spatial variations of microplankton communities were more pronounced than temporal ones; (2) samples from the same station basically clustered together, cor-responding to the geographic distribution of the sampling sites; (3) diversity derived from genetic and morpho-logical data did not correspond with each other well.     

1998年季风爆发期南海大气边界层的日变化

在南海尚未有关于海洋大气边界层结构日变化方面的研究.研究海洋大气边界层,有利于我们更好地研究海洋表层结构变化影响机制,对发生在海洋大气边界层上的过程进行预测预报.因此了解南海海洋大气边界层的结构特征,对于我们进一步了解南海天气尺度海气相互作用有非常重要的意义.通过对1998年在南海南部(6°15′N,110°E)和南海北部(20°29′39″N,116°57′48″E)的南海季风试验中定点科考船释放一天四次的探空观测资料分析得出,季风爆发前海洋大气边界层存在规则的日变化,在中午达到深厚.季风爆发后南海北部大气边界层日变化消失,南海南部依然明显.分析表明对其日变化起重要影响的是短波辐射;潜热输送对大气边界层高度日变化影响不大.与大气边界层厚度日变化相对应,南海南部边界层内水汽日变化明显,而南海北部较不明显.     

海域勘界技术的应用

制订划界方案是海域勘界工作的核心工作,包括资料分析、初拟划界草案、草案协调和协商等阶段．大多数海域界线需要分段划分．勘界实践表明,不同界线及其不同区段的界线必须运用不同的划界技术,如垂直岸线法、水道（航道）中心线法、协议线法、习惯线法、中点连线法、用海界线法及岛礁中间线法等．草案协调是勘界工作的难点,必须采取背靠背协调、面对面协商及反复沟通等方法,促成双方达成共识．     

Seasonal dynamics of elemental sulfur in two coastal sediments

A spectrophotometric method for elemental sulfur (S⁰) analysis without interference from other reduced sulfur compounds was adapted for the use in reducing sediments. The S⁰ distribution in two coastal sediments was studied regularly from summer to winter and compared to factors regulating the S⁰ accumulation, such as redox potentials, the rate of bacterial sulfide production and the general sulfur chemistry. Dense coatings of sulfur bacteria developed on the sediment surface of a sulfuretum which had an S⁰ concentration of up to 41 μmol S cm^?3. The 2·5-mm thick bacterial coating contained 40% of all S⁰ in the sediment. A more typical marine sediment with a few cm thick oxidized surface layer had an S⁰ maximum of 1–3 μmol S cm^?3 at 2–4 cm depth. The S⁰ maximum in both sediments increased from summer to winter as the sediments gradually became more oxidized. The deeper layers maintained a low S⁰ concentration. Most of the S⁰ in the upper few mm of a laboratory sulfuretum was present inside sulfur bacteria and actively migrated up and down with the bacteria depending upon the changing light and oxygen conditions.     

南黄海“高富强”地震勘查技术及其应用

南黄海海相中—古生界具有厚度大、埋藏深、构造复杂、岩性纵向非均质性强、浅部强反射界面能量屏蔽、碳酸盐岩层内部能量弱等特点。青岛海洋地质研究所联合中石化海洋石油工程有限公司、上海物探分公司(原第一海洋地质调查大队),通过持续的科技攻关,逐渐形成了适用于研究区内地震地质条件的"高富强"(高覆盖次数、富低频、强震源)地震勘查技术,获得了较高品质的海相中—古生界反射资料。2015年在崂山隆起上落实了多个大型中—古生界圈闭构造,为区内油气调查与勘探的突破提供了技术支撑。