转基因技术在鱼类及对虾中的应用(英文)

作者对转基动物的出现背景及优点,转基因过程的基本程序,转基因鱼研究的现状,鱼类基因的大量克隆,基因启动子的快速分离技术,分析启动子活性的瞬时转基因测试,绿色荧光蛋白转基因鱼作为观赏鱼的潜力,转抗病基因的研究进展,转基因技术在对虾中应用的展望,作了较全面的叙述。     

养鱼污水中鱼和树脂—半透膜囊对铜吸收的对比研究

在实验室条件下对比了彩虹方头鱼和树脂－半透膜对养鱼污水中铜的吸收。同时在野外条件下对比了鲤鱼和树脂－半透膜对铜的吸收。研究结果表明,树脂－半透膜囊的铜吸收量与钱鱼的金属吸收量存在显的线性正相关关系,利用树脂－半透膜囊可以有铲地模拟鱼对重金属的长期累积吸收。     

基于多维度指数的雅鲁藏布江鱼类多样性评价及群落构建过程分析

雅鲁藏布江是我国西藏地区最大的河流,孕育着独特的水生生物资源。近年来随着人类活动的干扰与河流开发的推进,亟待对该流域水生生物多样性进行全面调查评估。本研究基于雅鲁藏布江全流域的鱼类资源调查数据,整合物种多样性、功能多样性和系统发育多样性3个维度12项指数,对该流域鱼类多样性进行评价分析。结果显示,24个调查样点中共采集到37种鱼类,隶属于3目7科24属;其中土著鱼类27种,外来鱼类10种;鲤形目鲤科鱼类为优势类群。基于Bray-Curtis相异度的层次聚类可将雅鲁藏布江鱼类群落划分为4组,表现为帕隆藏布汇口以上/以下江段及两个江段内干支流间存在较大差异,这与区域生物地理学过程及河流环境梯度密切相关。利用12项多样性指数对各组群的鱼类多样性进行评估,结果表明,除Simpson多样性指数、Pielou均匀度指数、平均配对种间系统发育距离指数和平均最近种间系统发育距离(MNTD)指数外,其他指数检测到组间的显著性差异。不同多样性指数之间的相关关系表现各异:与丰富度相关的多项指数间存在显著或极显著的正相关,功能离散度指数和MNTD指数与其他指数间多存在显著或极显著的负相关,表明不同类别的多样性指数各自具有独特的指示价值。基于群落功能性状结构和系统发育结构的检验结果显示,大多数样点驱动鱼类群落聚合的过程为种间竞争排斥,推测与雅鲁藏布江水体营养贫瘠、食物来源稀缺导致的种间营养竞争关系紧张有关。本文整合多维度多样性指数对雅鲁藏布江流域鱼类多样性及群落构建过程进行探究,以期为该流域鱼类资源保护和管理提供科学依据,也为应用多维度指数评价淡水鱼类多样性提供参考借鉴。     

POTENTIAL EFFECTS OF CLIMATE CHANGES ON AQUATIC SYSTEMS: LAURENTIAN GREAT LAKES AND PRECAMBRIAN SHIELD REGION

The region studied includes the Laurentian Great Lakes and a diversity of smaller glacial lakes, streams and wetlands south of permanent permafrost and towards the southern extent of Wisconsin glaciation. We emphasize lakes and quantitative implications. The region is warmer and wetter than it has been over most of the last 12000 years. Since 1911 observed air temperatures have increased by about 0·11°C per decade in spring and 0·06°C in winter; annual precipitation has increased by about 2·1% per decade. Ice thaw phenologies since the 1850s indicate a late winter warming of about 2·5°C. In future scenarios for a doubled CO₂ climate, air temperature increases in summer and winter and precipitation decreases (summer) in western Ontario but increases (winter) in western Ontario, northern Minnesota, Wisconsin and Michigan. Such changes in climate have altered and would further alter hydrological and other physical features of lakes. Warmer climates, i.e. 2 × CO₂ climates, would lower net basin water supplies, stream flows and water levels owing to increased evaporation in excess of precipitation. Water levels have been responsive to drought and future scenarios for the Great Lakes simulate levels 0·2 to 2·5 m lower. Human adaptation to such changes is expensive. Warmer climates would decrease the spatial extent of ice cover on the Great Lakes; small lakes, especially to the south, would no longer freeze over every year. Temperature simulations for stratified lakes are 1–7°C warmer for surface waters, and 6°C cooler to 8°C warmer for deep waters. Thermocline depth would change (4 m shallower to 3·5 m deeper) with warmer climates alone; deepening owing to increases in light penetration would occur with reduced input of dissolved organic carbon (DOC) from dryer catchments. Dissolved oxygen would decrease below the thermocline. These physical changes would in turn affect the phytoplankton, zooplankton, benthos and fishes. Annual phytoplankton production may increase but many complex reactions of the phytoplankton community to altered temperatures, thermocline depths, light penetrations and nutrient inputs would be expected. Zooplankton biomass would increase, but, again, many complex interactions are expected. Generally, the thermal habitat for warm-, cool- and even cold-water fishes would increase in size in deep stratified lakes, but would decrease in shallow unstratified lakes and in streams. Less dissolved oxygen below the thermocline of lakes would further degrade stratified lakes for cold water fishes. Growth and production would increase for fishes that are now in thermal environments cooler than their optimum but decrease for those that are at or above their optimum, provided they cannot move to a deeper or headwater thermal refuge. The zoogeographical boundary for fish species could move north by 500–600 km; invasions of warmer water fishes and extirpations of colder water fishes should increase. Aquatic ecosystems across the region do not necessarily exhibit coherent responses to climate changes and variability, even if they are in close proximity. Lakes, wetlands and streams respond differently, as do lakes of different depth or productivity. Differences in hydrology and the position in the hydrological flow system, in terrestrial vegetation and land use, in base climates and in the aquatic biota can all cause different responses. Climate change effects interact strongly with effects of other human-caused stresses such as eutrophication, acid precipitation, toxic chemicals and the spread of exotic organisms. Aquatic ecological systems in the region are sensitive to climate change and variation. Assessments of these potential effects are in an early stage and contain many uncertainties in the models and properties of aquatic ecological systems and of the climate system. © 1997 John Wiley & Sons, Ltd.     

夏、冬两季真鲷仔、稚、幼鱼消化酶活性的比较

通过夏、冬两季真鲷仔、稚、幼鱼蛋白酶、淀粉酶和脂肪酶活性的变化研究表明,夏、冬两季3种消化酶变化趋势基本相似。蛋白酶的活性冬季比夏季低1个数量级;淀粉酶的活性冬季比夏季低1倍左右;脂肪酶的活性冬季高于夏季,且一直维持在相对稳定的水平。结果表明,3种酶活性受温度影响外,冬季脂肪酶的高活性对鱼苗的生长、成活具有重要意义。     

我国水库综合养鱼的发展前景

本文从分析水库综合养鱼生态系统的生态关系入手,阐述了水库综合养鱼的内容、意义、理论依据以及发展水库综合养鱼的自然条件和实践基础,并对综合养鱼系统的鱼产潜力进行了预测。预测结果表明,水库发展综合养鱼比传统的粗养方式其单位面积鱼产量可获得大幅度提高。     

黄河口及其邻近水域春季东亚江豚分布规律

东亚江豚(Neophocaena asiaeorientalis sunameri)是渤黄海水域仅有的常见鲸类动物,为我国二级保护水生野生动物,在世界自然保护联盟(International Union for Conservation of Nature,IUCN)物种名录中被列为濒危级.渤海黄河口及其邻近水域是东亚...     

汉江中下游梯级枢纽联合生态调度对产漂流性卵鱼类自然繁殖的效应研究

为促进汉江中下游鱼类繁殖,2018年6月汉江中下游首次实施梯级联合生态调度试验.该研究在汉江中下游钟祥、沙洋、泽口、仙桃江段设置监测断面,采集鱼类早期资源,并在兴隆枢纽泄洪闸上、下开展鱼类溯流集群情况监测,以分析生态调度对促进汉江中下游鱼类繁殖的效果.监测结果表明汉江中下游鱼卵种类共有26种(属),其中产漂流性卵鱼类22种.监测期间漂流性卵径流量为143411万粒,其中四大家鱼卵径流量为4887万粒,占3.41%,推测汉江中下游有6处成规模的产漂流性卵鱼类产卵场.生态调度期间,坝下鱼类溯流集群随兴隆枢纽调度过程发生变化,钟祥和仙桃江段均出现两次鱼类产卵高峰,鱼卵径流量占总径流量的61.66%,并监测到四大家鱼卵,表明梯级联合生态调度结合区间来水,对汉江下游鱼类,特别是四大家鱼的繁殖具有积极的作用.     

鄱阳湖通江水道鱼类空间分布特征及资源量评估

于2014年9月24至26日在鄱阳湖通江水道湖口至屏峰段使用SIMRAD EY60回声探测仪对鱼类资源进行水声学调查.结果显示,鄱阳湖通江水道鱼类目标强度平均值为-56.4±6.4 d B,范围为-69.9~-32.1 d B;估算的平均全长为7.6 cm,范围为1.2~98.0 cm;鱼类资源的平均密度为53.7 ind./1000 m3,范围为0~441.7 ind./1000 m3.在水平分布上,鱼类主要分布在湖口县、鞋山和屏峰附近3个水域.区域Ⅰ和区域Ⅲ的鱼类密度显著高于区域Ⅱ的鱼类密度;区域Ⅲ的鱼类个体大小显著小于区域Ⅰ和区域Ⅱ.在垂直分布上,多数鱼类栖息于主河槽的深水区,且大个体更倾向于深水区.在此基础上,利用Arc GIS地统计插值方法估算鄱阳湖通江水道湖口至屏峰山水域鱼类总数量约为6.2×107ind.,总生物量约620 t.结果表明,鄱阳湖通江水道是鱼类重要的栖息地,建议加强该水域的保护,保持鄱阳湖与长江的自然连通.