养护期介入电迁阻锈保障混凝土耐久性试验研究

沿海地区氯盐侵蚀会对混凝土结构造成严重的耐久性问题,混凝土设计使用寿命难以保障。对于新建工程,若在自由水含量较高混凝土养护期进行电迁阻锈处理,则有利于电迁移性阻锈剂在钢筋周围聚集以达到更佳的阻锈效果。为此,针对两种电迁移性阻锈剂对处于氯盐侵蚀环境中的混凝土适用性展开研究,并测试了干湿循环前后混凝土内钢筋电化学参数、氯离子扩散特征及钢筋周围电迁移性阻锈剂含量研究两种电迁移性阻锈剂对混凝土耐久性保障的作用。结果表明,试验中养护期介入电迁阻锈后试件阻锈效果良好,经过干湿循环后钢筋腐蚀电位正向高于-162.6 m V,同时,钢筋附近出现明显的阻锈剂聚集现象,能保持钢筋的钝化状态。     

西湖硅藻对桡足类浮游动物繁殖的影响

本文报道从杭州西湖筛选分离的两种淡水硅藻,即尖针杆藻Ｓｙｎｅｄｒａａｃｕｓ和谷皮菱形藻Ｎｉｔｚｓｃｈｉａｐａｌｅａ对西湖两种淡水浮游桡足类,即近亲拟剑水蚤Ｐａｒａｃｙｃｌｏｐｓａｆｆｉｎｉｓ和大尾真剑水蚤Ｅｕｃｙｃｌｏｐｓｍｕｃｒｒｏｉｄｅｓ卵孵化率的影响。结果表明,在较高密度的硅藻培养液（１．２×１０４～５．４×１０４个／ｃｍ３）中培养的桡足类动物,其卵的孵化率比生活在自然水体中的有明显降低,并发现该密度的实验硅藻对其摄食者——桡足类的母体也产生一定的毒害作用。该实验结果与已报道的某些海洋硅藻对海洋桡足类存在毒害作用的结果,对水域生态系食物链（网）中硅藻作用的传统概念提出了挑战,对水域生态学研究提出了新课题。     

An exploratory study on the use of enzyme activities as sediment tracers: biochemical fingerprints?

Little information exists on the potential of soil enzyme activities,which are sensitive to soil properties and management,for the characterization of sediment sources at the catchment scale.The objective of this study is to explore and evaluate enzyme activity as tracer for sediment fingerprinting in the Hiv catchment(55 km~2),Iran.Therefore,four enzymes were measured from 42 different sampling sites,covering three sediment source areas(rangeland/surface erosion,orchard/surface erosion,and streambank erosion),as well as from 12 sediment samples from reservoir check dams (sediment sinks).The results indicate that,based upon backward mode discriminant analysis,βglucosidase and dehydrogenase,allowed more than 95%of the samples to be correctly assigned to their source areas.These enzymes were selected as input data for a mixing-model to determine the relative contribution of the sampled sediment sources.The mean contributions from rangeland, orchard and streambank sources in the study area were estimated as 11.3%,15.1%and 73.7%, respectively.Using geochemical tracers,the mean contribution from rangeland,orchard and streambank sources was estimated as 14.1%,9.5%and 74.8%,respectively.Combined biochemical and geochemical tracers,similar values were obtained(18.7%,10.7%and 70.7%,respectively).Our results indicate that soil enzyme activity allows for a good characterization of sediment sources,and can provide a complementary tool to currently existing sediment fingerprinting approaches.However, the method should be also tested in other regions.     

Effect of Agents on Hydrate Formation and Low-Temperature Rheology of Polyalcohol Drilling Fluid

In order to ensure safe drilling in deep water and marine gas hydrate bearing sediments,the needed characteristics of drilling fluid system were analyzed.Moreover,the effect of different agents on hydrate formation and the low-temperature rheology of designed polyalcohol drilling fluid were tested,respectively.The results show that clay can promote gas hydrate growth,while modified starch and polyalcohol can inhibit hydrate formation to some extent,and PVP K90 has a good performance on hydrate inhibition.Th...     

低土相高密度钻井液体系的配方研制

针对高密度钻井液体系普遍存在的黏度偏大、流变性难以调控、经常出现井下复杂情况等问题,通过对聚合物包被剂、膨润土含量、润滑剂、抑制剂的优选,确定了低土相高密度钻井液体系的最佳配方,开发了一套低土相高密度钻井液体系。室内研究结果表明,该钻井液体系具有良好的抗钻屑、NaCl和石膏的污染能力和良好的抑制封堵能力,岩屑滚动回收率达到93．7％,能够满足高温高压、严重造浆、高渗透率以及微裂缝发育等复杂地层的钻井技术要求。     

The Ecological Restoration of Heavily Degraded Saline Wetland in the Yellow River Delta

As a result of discontinuous water flow, agriculture, and increasing urban use of fresh water affecting the natural wetlands of the Yellow River Delta, these areas have experienced significant degradation in the past two decades, ultimately diminishing the overall natural wetland land area in the region. This study aimed to address the issue of decreasing fresh water in the Yellow River Delta by studying the effects of three different approaches to restoration on long‐term wetland recovery. The results of the study demonstrated that soil salt and available Na contents significantly decreased in response to all three restoration treatments. Impacts of the restoration treatments were more significant in 2009 than in 2010, as shown by the high rate of activity in the reed debris group. The highest phosphatase activity of the experimental period was also observed in the reed debris group. Meanwhile, a marked variation in soil nutrient elements (total carbon (TC), total nitrogen (TN), available phosphorus, and available potassium) was observed in the restoration treatment plots throughout the experimental period. TC and TN contents were generally higher in the restoration treatment groups than in the control group. Moreover, urease and phosphatase activity levels were highly correlated with one another, as well as with soil nutrient elements. In 2009, the yield of the Suaeda salsa plant was highest in the reed debris treatment group and lowest in the ploughing treatment group. The S. salsa plant did show a positive response to all of the different restoration treatments. Taken together, these results suggest that restoration approaches that implement ploughing techniques aided in the restoration of degraded saline wetlands.     

Biodegradation of Herbicide Propanil and Its Subproduct 3,4‐Dichloroaniline in Water

The rational use of pesticides generates an impact which is normally reversed and eliminated by the environment itself. However, the indiscriminate use of pesticides makes its natural degradation rhythm difficult, prolonging their presence in the soil for a great deal of time. Aiming towards a decrease in the environmental impact of pesticides, soil microorganisms capable of degrading pesticides, such as propanil, were investigated. An Enterobacter cloacae strain, isolated from rice field soil, was exposed to the herbicide propanil alone and in a mixture containing also bentazone, clomazone, quinclorac, and 2,4‐D. This bacterium was able to eliminate 100% of the applied propanil in 28 days. Propanil degradation in the 5‐herbicide mixture was much lower than that of individual pesticide degradation. The aeration of the system helped to degrade propanil and its subproduct 3,4‐dichloroaniline much faster. LC with UV detection was used to determine the remaining concentrations of the herbicides and their subproducts.     

鳗弧菌(Vibrio anguillarum)侵染对青蛤(Cyclina sinensis)谷胱甘肽硫转移酶及其基因表达的影响

在鳗弧菌胁迫下测定了青蛤血清及肝脏谷胱甘肽硫转移酶(GSTs)活力,利用SMART cDNA文库和高通量测序方法,筛选到青蛤σ型谷胱甘肽硫转移酶基因(CsGSTS)的全长。采用荧光定量PCR法分析CsGSTS基因的表达过程。结果表明,青蛤的CsGSTS基因cDNA全长793bp,编码206个氨基酸,具典型的GST-N和GST-C结构域。血清中的GSTs活力在感染后6—24h显著升高(P<0.01),肝脏GSTs活力在6—12h显著下降(P<0.05),48—96h又明显上调(P<0.01);肝脏CsGSTS基因表达水平在胁迫后3—6h降低,24h显著升高,48h达到最大值,约为对照组的3倍。说明谷胱甘肽硫转移酶及其基因表达参与了青蛤的免疫应答反应。该研究为探索贝类的抗病害免疫机制提供了重要的实验数据。