Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China （LCDV-cn） genome

An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene. Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309)     

灰色关联评价在矿区地下水水质分析中的应用

利用灰色系统理论的灰色关联分析方法,根据灰色关联矩阵提供的丰富信息对地下水水质进行综合评价。通过比较分析,灰色评价方法计算过程简单方便,又充分利用获得的信息,且结果直观可靠,是多因子综合评价较好的方法。     

Laboratory study of deep-water breaking waves

In an attempt to elucidate the mechanics of deep-water wave breaking, a variety of breaking waves, including spilling and plunging waves, of different length scales and geometries was studied. The waves were generated through wave-wave interactions using wave packets with constant-steepness components, constant-amplitude components, and also components following the Pierson-Moskowitz distribution. Wave steepening prior to breaking were found to cause an increase in the high frequency spectral slope of the wave spectrum. The slopes were correlated to the type of breaking and the intensity of the breaking. The energy loss through breaking varied with the spectral characteristics of the wave packet. On the other hand, it was also noted that, irrespective of the wave packet, the losses were from the higher frequency end of the first harmonics.     

Estimated mass and stiffness matrices of shear building from modal test data

A method that estimates mass and stiffness matrices of shear building from modal test data is presented in this paper. The method depends on only measurable points that are less in number than the total structural degrees of freedom, and on the first two orders of structural mode measured. So it is applicable to most of the general test. Based on this method modal data of unmeasurable points are estimated, then global mass and stiffness matrices of structure are obtained by using the first two orders of modal data. Taking advantage of iteration the optimum global mass and stiffness matrices are gained. Finally, an example is studied in this paper. Its result shows that this method is reliable. © 1998 John Wiley & Sons, Ltd.     

Finite element analysis of the effect of corrosion on the behavior of reinforced earth walls

A simple model for the corrosion‐induced loss of stiffness and strength of the steel strips of earth‐reinforced walls was introduced in a finite element simulation of the long‐term behavior of the wall, in which the backfill‐strips interactions are taken into account by means of a generalized homogenization procedure (called a multiphase model). The results show an initial phase of slow displacements induced by the loss of stiffness, followed after a few decades by a steep acceleration of the displacements, leading to wall failure. The influences of the parameter controlling corrosion, the backfill cohesion and the heterogeneity of the corrosion process are discussed. Results are used to discuss a strategy for reinforced earth wall surveillance. Copyright © 2011 John Wiley & Sons, Ltd.     

Application of saddlepoint approximation in reliability analysis of dynamic systems

The application of the saddlepoint approximation to reliability analysis of dynamic systems is investigated. The failure event in reliability problems is formulated as the exceedance of a single performance variable over a prescribed threshold level. The saddlepoint approximation technique provides a choice to estimate the cumulative distribution function (CDF) of the performance variable. The failure probability is obtained as the value of the complement CDF at a specif ied threshold. The method requires computing the saddlepoint from a simple algebraic equation that depends on the cumulant generating function (CGF) of the performance variable. A method for calculating the saddlepoint using random samples of the performance variable is presented. The applicable region of the saddlepoint approximation is discussed in detail. A 10-story shear building model with white noise excitation illustrates the accuracy and effi ciency of the proposed methodology.     

Point-block covariance: A general solution for the two-dimensional stationary random functions

The application of the theory of random functions to problems of ore evaluation may involve computations of the covariance between the mean value of a given block and the functional value at a given point. However, an analytical solution for such a covariance does not exist for nonspherical blocks and for commonly applied models of covariance functions. Further, because this covariance is a function of the spatial arrangements of the block and the point, it has to be evaluated numerically each time for given point—block arrangements. This paper presents a readily available general solution to this problem in the form of a series of graduated curves which, together with some geometric manipulations, may be used to compute the covariance between a pointand a two-dimensional block for all possible point—block arrangements. The availability of the graph thus eliminates the necessity of using the time-absorbing programs on computers for such computations. Finally, many of the approximations that are made in order to avoid cumbersome covariance evaluations are no longer necessary due to the ease of such computations with the help of the graph provided.     

基于Matlab的断裂带温泉水地球化学特征及地震活动性研究

基于Matlab开发出地下流体分析软件,对比分析了甘肃西秦岭北缘的武山、街子和清水温泉水化学组分特征;初步分析了温泉水水质类型、补给来源、水-岩平衡状态以及循环深度,并讨论了温泉水深循环对地震活动性的影响.研究表明温泉水均为大气降水成因,其水化特征受围岩的控制作用;清水温泉水的成熟度没有武山和街子温泉水高.地下水循环深度较浅的武山温泉和街子温泉水化类型属于Na-HCO3·SO4·Cl,所处断裂的地震活动频繁;地下水循环深度较深的清水温泉水化类型属于Na-SO4·Cl,所处断裂的地震活动性弱.     

The Coral Triangle Initiative and regional exchanges: Strengthening capacity through a regional learning network

Natural resource management and conservation programs that promote building capacity and social learning among participants often lead to the formation of learning networks: a type of social network where learning is both a goal and potential outcome of the network. Through forming relationships and sharing information, participants in a learning network build social capital that can help a network achieve social and environmental goals. In this study, we explored social capital in a learning network that emerged through a large-scale marine governance effort, the Coral Triangle Initiative on Coral Reefs, Fisheries, and Food Security. Through a mixture of social network analysis and key informant interviews, we examined the major patterns of information exchange among individuals who had participated in regional learning exchanges; evaluated whether the network's structure resulted in information sharing; and considered implications for strengthening network sustainability, capacity building, and learning. We found that the Regional Exchange network fostered information sharing among participants across national and organizational boundaries. While the network had individuals who were more central to information sharing, the network structure was generally decentralized, indicating potential resilience to changes in leadership and membership. Participants stressed the importance of the knowledge and connections they had acquired through the learning network; however, they expressed doubts regarding its sustainability and stressed the need for a strong coordinating entity. Our findings suggest that conservation learning networks have the ability to bridge cultural divides and promote social learning; however, a strong network coordinator and continuing efforts to support information sharing and learning are crucial to the network's strength and sustainability. The tangible learning and capacity development outcomes cultivated through Regional Exchange network underscore the value of and need to invest in conservation networks that support peer-to-peer learning.     

搅拌桩复合地基平面模拟的简化方法探讨

水泥土搅拌桩(DMM)在高速公路工程中得到广泛应用,人们也希望能对该复合地基在路堤荷载下的地基的变形深入了解,其数值分析是一种重要的方法。但是,采用三维有限元进行数值模拟在高速公路工程中容易造成数组过大,计算时间并且收敛性比较差,因此把三维问题简化为二维问题是众望所归。在把三维问题简化二维平面问题简化计算时,最重要的是参数的折减。基于水泥土搅拌桩复合地基的特性,结合理论分析提出了一种参数折减的方法。并依据连盐高速公路实测的数据验证了该参数简化方法的可行性。