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81.
采用廉价的乳糖替代IPTG诱导重组别藻蓝蛋白(rAPC)在大肠杆菌(Escherichiacoli)JM109中表达,对诱导产物表达的培养基、培养条件、诱导剂的浓度和诱导时机进行了优化,将优化条件用于5L自控发酵罐进行高密度培养。结果表明,对rAPC表达的条件进行优化后,乳糖诱导rAPC的表达率可达26.8%,与IPTG的诱导结果接近;在高密度培养中,OD600最大达21.8。研究结果可为乳糖替代IPTG大规模生产rAPC奠定基础。 相似文献
82.
针对以往噪声信号发射换能器在实际工作中,存在着诸多影响正常科研试验的问题,并根据噪声信号的技术要求,以及探测方式的技术特征,我们对噪声发射换能器进行了重新设计,从选用超磁致伸缩材料作为换能器的材料入手,结合国内外一些新的设计理念,从材料性能分析,到理论设计,最后到可靠性实施方法,都进行了周密细致的研究,并完成了设计全过程。测试结果和使用性能表明,达到了预期的目的,这也是该材料在国内首次应用于单只宽频水声换能器中,开创了稀土超磁致伸缩材料应用的又一个先列。 相似文献
83.
The aim of this paper is to investigate the shape and tension distribution of fishing nets in current. A numerical model is developed, based on lumped mass method to simplify the net. The motion equation is set up for each lumped mass. The Runge–Kutta–Verner fifth-order and sixth-order method is used to solve these simultaneous equations, and then the displacement and tension of each lumped mass are obtained. In order to verify the validity of the numerical method, model tests have been carried out. The results by the numerical simulation agree well with the experimental data. 相似文献
84.
Claudio Campagna Alberto R. Piola Maria Rosa Marin Mirtha Lewis Teresita Fernndez 《Deep Sea Research Part I: Oceanographic Research Papers》2006,53(12):1907-1924
This study describes the association between transient, mesoscale hydrographic features along the axis of the Brazil–Malvinas Confluence, in the SW Atlantic, and the foraging behavior of 2–3-year-old (focal) juvenile southern elephant seals, Mirounga leonina, from Península Valdés, Argentina. Departing from the dominant pattern of foraging on predictable bathymetric fronts on the Patagonian shelf and slope, three females out of 12 satellite-tracked juveniles remained at the edge of young warm-core eddies and near the outer core of cold-core eddies, coinciding with the most productive areas of these temperature fronts. Seal trajectories along high-temperature gradients were always consistent with the speed and direction of surface currents inferred from the temperature distribution and confirmed by surface drifters. Movements of foraging seals were compared with those of surface drifters, coinciding in time and space and yielding independent and consistent data on regional water circulation parameters. The diving pattern recorded for one focal seal yielded shallower dives and a loose diel pattern in the eddy, and a marked diurnal cycle compatible with foraging on vertically migrating prey in the cold waters of the Malvinas Current. Pre-reproductive females that use the mesoscale fronts of the Argentine Basin as an alternative foraging area would benefit from lower competition with more experienced seals and with other top predators that reproduce along the coast of Patagonia. 相似文献
85.
An optimization mathematical model of the pile forces for piled breasting dolphins in the open sea under various loading conditions is presented. The optimum layout with the well distributed pile forces and the least number of piles is achieved by the multiplier penalty function method. Several engineering cases have been calculated and compared with the result of the conventional design method. It is shown that the number of piles can be reduced at least by 10%—20% and the piles‘ bearing state is improved greatly. 相似文献
86.
Shigenao Maruyama Koutaro Tsubaki Keisuke Taira Seigo Sakai 《Journal of Oceanography》2004,60(3):563-568
Deep seawater in the ocean contains a great deal of nutrients. Stommel et al. have proposed the notion of a “perpetual salt fountain” (Stommel et al., 1956). They noted the possibility of a permanent upwelling of deep seawater with no additional external energy source. If
we can cause deep seawater to upwell extensively, we can achieve an ocean farm. We have succeeded in measuring the upwelling
velocity by an experiment in the Mariana Trench area using a special measurement system. A 0.3 m diameter, 280 m long soft
pipe made of PVC sheet was used in the experiment. The measured data, a verification experiment, and numerical simulation
results, gave an estimate of upwelling velocity of 212 m/day.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
87.
Experimental study of strong reflection of regular water waves over submerged breakwaters in tandem 总被引:3,自引:0,他引:3
A series of laboratory experiments was carried out to investigate the strong reflection of regular water waves over a train of submerged breakwaters. Rectangular and trapezoidal shapes of submerged breakwaters are employed and compared for reflecting capability of incident waves. Measured reflection coefficients of regular waves over impermeable submerged breakwaters are verified by comparing with those of the eigenfunction expansion method. A very good agreement is observed. Reflection coefficients of permeable submerged breakwaters are less than those of impermeable breakwaters. The trapezoidal shape is recommended for a submerged breakwater in terms of reflecting capability and practical application. 相似文献
88.
《海洋技术学报》2024,(1)
利用传感器对水体叶绿素a 浓度进行原位测量是获取实时、连续、长时间序列数据的重要手段。本文在对RBR 传感器和ECO(Environmental Characterization Optics)传感器进行原理分析和线性度、稳定性、重复性等基本性能测试的基础上,利用单一藻种培养液和2020年南海北部海域现场数据校准传感器,并对新的传感器校准系数进行验证。结果表明:两台传感器使用新系数比原出厂系数的叶绿素a 浓度测量准确度有明显提高。RBR 传感器现场数据校准系数的计算结果与叶绿素a 标准值误差最小,平均绝对误差从1.93 μg/L 减小到0.35 μg/L,平均相对误差从55.1%减小到10.9%;ECO 传感器藻液系数明显优于出厂系数和现场数据校准系数的计算结果,平均绝对误差从1.76 μg/L 减小到0.59 μg/L,平均相对误差从50.3%减小到15.1%。传感器测量准确度的提高,可为海洋环境监测、海洋生态灾害预警等工作获取真实可靠数据提供支撑。 相似文献
89.
Tang Rongqing Wang Daoyan Zhang Xixian Egnineer Shanghai Jiao Tong University Shanghai Associate Professor Shanghai Jiao University Shanghai 《中国海洋工程》1993,(3)
- The authors have used a low-cost ROV of observation type for underice surveying of the Nen-Jiang Cross-river oil pipe line in the Da Qing oil field. The operation method, process and the safety measures for severe cold areas are presented. The fruitful tries and experience may be useful to ROV operators in severe cold areas. 相似文献
90.
MO Jiaqi * WANG Hui LIN Wantao LIN Yihua. Anhui Normal University Wuhu China. Division of Computional Science E-Institute of Shanghai Universities Shanghai Jiaotong University Shanghai China. Chinese Academy of Meteorological Sciences Beijing China. State Laboratory of Numerical Modeling for Atmospheric Sciences Geophysical Fluid Dynamics Institute of Atmosheric Physics Chinese Academy of Sciences Beijing China 《海洋学报(英文版)》2005,(5)
1 IntroductionThe El Ni’o atmospheric physics oscillation is anabnormal phenomenon involved in the tropical Pacificocean- atmosphere interactions. Studies on the El Ni(ophenomenon are very attractive (Lin et al., 2000; Linet al., 2001, 2002; Wang, 2001; Feng et al., 2001;Feng et al., 2002; Liu et al., 2002; Wei and Chen,2003; Xie et al., 2002; Zhu et al., 2002; Pu et al.,2003; Gu et al., 2004; Yu and Liu, 2004). Lin and Mo(2004), Mo and Lin (2004) and Mo et al. (2004) alsostudied a s… 相似文献