蓝隐藻藻蓝蛋白结构与功能稳定性研究

以蓝隐藻(Chroomonas placiodea)藻蓝蛋白 PC-645为材料,通过改变环境 pH 和尿素质量浓度,监测其变性和复性过程中特征荧光谱和吸收谱的动力学变化,以期了解隐藻藻蓝蛋白的色基和蛋白结构稳定性及其与功能的关系。结果表明, PC-645在很宽的 pH 范围和一定质量浓度的尿素中维持结构和功能的稳定,空间结构具有很强的柔性。pH诱导的 PC-645蛋白构象与功能变化可分为3个不同的区段。(1)稳定区(pH 3.5~7)：吸收和荧光光谱都比较稳定,显示蛋白质构象和功能在此区域都保持正常。(2)次稳定区(pH 7~10)：光吸收依然保持平稳,亚基内部的色基的状态和疏水微环境都没有改变,但荧光传递效率降低,可能是由亚基表面局部构象变化、解离(四级结构变化)或者色素基团间的空间距离变化引起。(3)不稳定区(pH<3.5和 pH>10),吸光度和荧光强度都呈快速下降,色基在近紫外和可见光区的吸收峰位变动,蛋白构象处于快速崩溃期。PC-645在酸性条件下的稳定性高于在碱性环境下,是与隐藻藻蓝蛋白所处的特殊环境及生理功能相适应的。     

北京城郊山地森林游憩机会谱构建

20世纪中叶,到森林中休闲游憩的人数剧增,为了保护生态环境,同时为游客提供高质量的游憩体验,美国林务局的管理者和专家发展了游憩机会谱(ROS)理念和管理框架,其主要以游憩需求为导向,依据"自然环境"、"社会环境"和"管理环境"指标对游憩区进行分类分级,编制游憩机会清单,对资源进行清查、规划和管理。本文在介绍ROS理论的基础上,结合北京市民森林游憩的需求特征和森林游憩区当前的管理体制和开发利用现状,筛选出"自然程度"、"偏远程度"、"游客密度"和"管理强度"4个评价指标,分析提出了北京山地森林游憩机会谱,将北京城郊山地森林游憩区分为城郊开发区域、城郊自然区域、乡村开发区域、乡村自然区域和半原始区域五个游憩机会等级,并结合目前的北京市游憩资源的利用和管理现状,用GIS作图直观展示了各游憩机会的分布,分析比较了各游憩机会的供需状况,为北京城郊森林游憩的规划和管理提出了合理化建议。     

缺损桩的导纳谱判别法及应用

利用振动工程中的模态分析技术,对桩基系统的振动特性进行研究。根据桩基系统的集中质量参数振动模型,对完整桩和各种缺损桩模型的导纳谱进行了大量的正演计算,按照导纳谱特征与桩基的缺损类型及缺损位置之间的对应关系,编制了相应的判读软件DSP1.0系统,实现了桩基完整性以及缺损桩缺损位置和缺损性质的计算机自动判读。     

The influence of Stokes drift on oil spills: Sanchi oil spill case

Spilled oil floats and travels across the water’s surface under the influence of wind, currents, and wave action. Wave-induced Stokes drift is an important physical process that can affect surface water particles but that is currently absent from oil spill analyses. In this study, two methods are applied to determine the velocity of Stokes drift, the first calculates velocity from the wind-related formula based upon a one-dimensional frequency spectrum, while the second determines velocity directly from the wave model that was based on a two-dimensional spectrum. The experimental results of numerous models indicated that: (1) oil simulations that include the influence of Stokes drift are more accurate than that those do not; (2) for medium and long-term simulations longer than two days or more, Stokes drift is a significant factor that should not be ignored, and its magnitude can reach about 2% of the wind speed; (3) the velocity of Stokes drift is related to the wind but is not linear. Therefore, Stokes drift cannot simply be replaced or substituted by simply increasing the wind drift factor, which can cause errors in oil spill projections; (4) the Stokes drift velocity obtained from the two-dimensional wave spectrum makes the oil spill simulation more accurate.     

MK morphological study of Am stars at 66 Å/mm

The pseudo-luminosity effect in the metallic line A-type stars found by Abt & Morgan (1976) is confirmed in a random sample of 27 Am stars. From a morphological study of their spectra in the wavelength interval 3850-4400 Å at a reciprocal dispersion of 66 Å/mm, revised spectral types are given on the MK system for their K-line and metallic-line spectra. This shows that: (a) our segregation of weak Am from the Am stars largely agrees with that by Cowleyet al. (1969); (b) all the stars in the sample are dwarfs according to their K-line classification; (c) more than 80 per cent exhibit the pseudo-luminosity effect significantly, with their metallic-line spectra resembling a giant or even a supergiant in the violet (3850-4100 Å), and a giant rather than a dwarf in the blue region (4260-4400 Å); (d) in two-thirds of the stars under (c), the Sr n 4077 Å line is found to have a markedly brighter luminosity class compared to any region, and in more than one-third of the sample it is comparable to that in Ap stars; (e) at least five stars exhibit characteristics which might suggest a spectrum variability: among these, the most striking example is 41 Sex A which was found to show a phase-modulated spectrum variation hitherto unknown in Am stars; (f) the metallic-line spectra of another five stars appear to be similar to A-shell type in differing degrees; (g) less than 20 per cent of the sample comprises stars which do not show any significant differential luminosity effect; these stars might have been misclassified or perhaps they are in a quiescent state. We also confirm the conclusion arrived at by Böhm-Vitense & Johnson (1978) that all Am stars may vary and our observations suggest that groups may exist among them.     

Modelling combined open channel flow by artificial neural networks

Combined open channel flow is encountered in many hydraulic engineering structures and processes, such as irrigation ditches and wastewater treatment facilities. Extensive experimental studies have conducted to investigate combined flow characteristics. Nevertheless, there is no simple relationship that can fully describe the velocity profiles in a turbulent flow. The artificial neural network (ANN) has great computational capability for solving various complex problems, such as function approximation. The main objective of this study is to evaluate the applicability of the ANN for simulating velocity profiles, velocity contours and estimating the discharges accordingly. The velocity profiles measured by an acoustic doppler velocimeter in the open channel of the Chihtan purification plant, Taipei, with different discharges at fixed measuring section and different depths are presented. The total number of data sets is 640 and the data sets are split into two subsets, i.e. training and validation sets. The backpropagation algorithm is used to construct the neural network. The results demonstrate that the velocity profiles can be modelled by the ANN, and the ANN constructed can nicely fit the velocity profiles and can precisely predict the discharges for the conditions investigated. Copyright © 2005 John Wiley & Sons, Ltd.     

EFFECT OF WATER DEPTH ON WIND-WAVE FREQUENCY SPECTRUM Ⅰ.SPECTRAL FORM

Wen et al.'s odhed dewiogh to obtain wind-wave frequency spectrum in deep was used toderive the spectrum in finite depth water. The spedrum S(ω) (ω bein angular frequency) when normalizedwith the zeroth moment m and peak frequercyω。 contains in adrition to the peakness factor P=ω。S(ω。)/m。, a twth parameter n=(2πm。)_(1/2)d (d being water depPth), so the spatrum behavior can bestudies for different ware growth stages and water depths.     

714C气象雷达电磁干扰的分析及解决方法

雷达设备电磁干扰的解决方法,依赖于对干扰的准确分析。通过昆明机场７１４Ｃ气象雷达受空军警戒雷达干扰的实例,介绍了一种高功率脉冲对雷达发射机干扰的分析过程,并提出解决方法。     

Thickness estimates of the upper-mantle transition zone from bootstrapped velocity spectrum stacks of receiver functions

We modify the receiver-functions stacking technique known as velocity spectrum stacking (VSS) so as to estimate combinations of velocity model ( V_P and V_S ) and depth that stack the Ps conversion from upper-mantle discontinuities most coherently. We find that by estimating the differences in the depths to the 660 and 410 km discontinuities using velocities that maximize the stacked amplitudes of P410s and P660s phases we can estimate the thickness of the transition zone more accurately than the depths to either of these discontinuities. We present two examples indicating that the transition zone beneath Obninsk, Russia, is 252±6 km thick and that beneath Pasadena, California, is only 220±6 km thick.     

太阳活动区磁重联过程中电场加速高能电子分布的演化特征

基于活动区暗条快速上升后其下方电流片重联过程中产生强电场加速电子这一物理过程,解包括库仑碰撞和离子声湍动散射的Fokker-Planck方程,获得电子分布函数的演化特征．计算结果表明(1)当电场强度为1-10V·cm-1时,在10-5-10-6s可将电子加速到(0．1-1)Mev量级;(2)被加速电子近似呈幂律谱分布,其谱指数与耀斑脉冲相微波和硬X射线推断的电子谱指数基本吻合．