Contribution of trimethylamine N-oxide on the growth and pressure tolerance of deep-sea bacteria

Trimethylamine N-oxide(TMAO) is widely dispersed in marine environments and plays an important role in the biogeochemical cycle of nitrogen. Diverse marine bacteria utilize TMAO as carbon and nitrogen sources or as electron acceptor in anaerobic respiration. Alteration of respiratory component according to the pressure is a common trait of deep-sea bacteria. Deep-sea bacteria from dif ferent genera harbor high hydrostatic pressure(HHP) inducible TMAO reductases that are assumed to be constitutively expressed in the deep-sea piezosphere and facilitating quick reaction to TMAO released from ?sh which is a potential nutrient for bacterial growth. However, whether deep-sea bacteria universally employ this strategy remains unknown. In this study, 237 bacterial strains affliated to 23 genera of Proteobacteria,Bacteroidetes, Firmicutes and Actinobacteria were isolated from seawater, sediment or amphipods collected at dif ferent depths. The pressure tolerance and the utilization of TMAO were examined in 74 strains. The results demonstrated no apparent correlation between the depth where the bacteria inhabit and their pressure tolerance, regarding to our samples. Several deep-sea strains from the genera of Alteromonas, Halomonas,Marinobacter, Photobacterium, and Vibrio showed capacity of TMAO utilization, but none of the isolated Acinebacter, Bacillus, Brevundimonas, Muricauda, Novosphingobium, Rheinheimera, Sphingobium and Stenotrophomonas did, indicating the utilization of TMAO is a species-speci?c feature. Furthermore, we noticed that the ability of TMAO utilization varied among strains of the same species. TMAO has greater impact on the growth of deep-sea isolates of Vibrio neocaledonicus than shallow-water isolates. Taken together, the results describe for the ?rst time the TMAO utilization in deep-sea bacterial strains, and expand our understanding of the physiological characteristic of marine bacteria.     

Experimental investigation of tsunami bore impact pressure on a perforated seawall

Catastrophic failures of many tsunami barriers along the affected coasts during the 2011 Tohoku earthquake tsunami has prompted extensive investigation into improving and revising design codes for tsunami defence structures. To date, researchers and coastal engineers are investigating to understand the failure mechanisms and to find solutions so that the structures merely remain intact in the extreme event such as tsunami. Thus, the present work is motivated to experimentally study tsunami-induced bore pressures exerted on vertical seawalls; a solid vertical wall and a porous vertical seawall that consisted of a perforated front wall and a solid rear wall. Bores with various heights and velocities were generated by using the dam-break method. A porous seawall with 20% porosity of perforated front wall was used in this study. Bore pressures exerted on the solid rear wall and chamber oscillations that occurred in the experiments were also discussed. The experimental results showed that multiple peak pressures were observed during bore run-up phase in the time series of bore impacts. A predictive equation to estimate the maximum bore pressure on a perforated seawall was developed using multiple regression analysis. The proposed equation was also compared with previous empirical formulas.     

Effects of long-term cyclic horizontal loading on bucket foundations in saturated loose sand

A 1-g model experimental study was conducted to investigate the accumulated rotations and unloading stiffness of bucket foundations in saturated loose sand. One-way horizontal cyclic loading was applied to model bucket foundations with embedment ratios 0.5 and 1.0. Up to 10⁴ cycles of loading were applied at a frequency of 0.2 Hz varying load amplitudes. The accumulated rotation of the bucket foundations increased with the number of cycles and the load amplitudes. Empirical equations were proposed to describe the accumulated rotation of the foundations. The unloading stiffness of foundations increased with the number of cycles but decreased with an increase in load amplitude. The initial unloading stiffness of L/D = 1.0 (L is skirt length; D is foundation diameter) was approximately twice that of L/D = 0.5. Excess pore water pressure difference of 50% was observed between L/D = 0.5 and 1.0. The suction and static capacity of the bucket increased with increase of bucket embedment ratio with a difference of 69.5% and 73.6% respectively between L/D = 0.5 and 1.0.     

A modified numerical calculation method of collapse pressure for thick-walled offshore pipelines

Considering the shear deformation and thickness stretching of large deformation, a modified numerical calculation method based on the thick shell theory is established to determine the collapse pressure of thick-walled pipes. Verification experiments are conducted on ten pipe specimens in hyperbaric chambers. The good agreement between experimental results and numerical predictions shows the validity and reliability of the new numerical calculation method. Combining DNV specification, the characteristic collapse pressure is also calculated for comparison. The difference between experimental results and DNV calculations illustrates the latter one is much conservative in predicting collapse pressure for thick-walled pipes. Sensitivity analysis on manufacturing imperfections and material properties is investigated for pipes with different D/t ratios. Thick-walled pipes are easier to be affected by initial ovality, residual stress and hardening factor. Based on the stress distribution at the moment of collapse, a novel discovery is found that the collapse pressure of thick-walled pipes is dominated by material plastic behavior.     

Effects of installation on the cyclic axial behaviour of suction buckets in sandy soils

Suction buckets differ with their easy and cost-efficient installation technique from other foundation types for offshore wind turbines. For successful completion of their installation process, suction is essential, but the imposed seepage leads to the changes in states of the soil in and around the bucket. Especially, a loosening of soil inside the bucket affects the load carrying behaviour of bucket subjected to repetitive loading resulting from environmental conditions. In this study, the behaviour of buckets under cyclic axial compressive loads with considering a possible loosening and related changes in permeability of soil inside the bucket is investigated numerically. In the framework of finite element analysis, a fully coupled two-phase model and a hypoplastic constitutive model are used to describe the saturated sandy soil behaviour under repetitive loading. The porosity-permeability variation is taken into account by Kozeny–Carman relationship. Special attention is dedicated to load carrying behaviour of bucket top plate, inner and outer skirt as well as base and their changes resulting from a loosening of soil inside the bucket with variable aspect ratio. For this purpose, cyclic axial compressive loads which cause an attenuation and progressive failure of soil-bucket system response are considered. The main findings on the changes in load carrying behaviour of bucket are presented and discussed.     

录井校正新方法在东海盆地西湖凹陷的应用

录井检测受井场环境影响较大,主要包括钻井环境与检测环境,钻井环境包括井径、钻时、钻井液排量、是否取心等,检测环境主要是井场自然环境与检测器内部环境。一般情况下检测器内部环境基本一致,而井场自然环境在一定时间内变化不大,故而钻井环境对录井检测的影响较大,钻井环境校正是进行录井解释前的重要工作。常规Tg(全烃含量)校正方法在取心、低异常、多互层等层段使用效果较差,经过分析,将校正方法在取心、钻时、排量、基值方面进行优化,实践验证,新校正方法效果较好,适应了西湖凹陷的地质研究需求。     

深海热液区主要成分的高温高压拉曼光谱分析

模拟深海热液口环境(最高压力40 MPa,最高温度350℃),对深海热液区的主要成分CO2、CH4及其混合物的水溶液在不同压力和温度条件下的拉曼光谱进行探测和分析,结果显示:常温低压下CO2水溶液的Fermi双峰分别位于1 384.9 cm-1和1 278.3 cm-1处,CH4的水溶液拉曼峰υ1位于2 912.1 cm-1处,均比其气相的拉曼频移小;常温下CO2和CH4水溶液的拉曼特征峰随压力(≤40 MPa)的变化均不明显;在40 MPa的压力下随着温度的升高(≤350℃),CO2水溶液的Fermi双峰分别向高波数区移动了约3.4 cm-1和7.0 cm-1,而CH4水溶液的拉曼峰υ1向低波数区移动了约3.1cm-1;混合后升温过程中CO2的双峰分别向高波数区移动了约4.3 cm-1和3.8 cm-1,CH4的特征峰υ1向低波数区移动了4.5 cm-1。说明在室温到350℃范围内温度的变化对CO2和CH4水溶液拉曼频移有影响,频移量与温度线性相关,而压力在≤40 MPa范围内的改变对拉曼频移影响不明显。     

Response of a porous seabed around breakwater heads

The evaluation of wave-induced pore pressures and effective stresses in a porous seabed near a breakwater head is important for coastal engineers involved in the design of marine structures. Most previous studies have been limited to two-dimensional (2D) or three-dimensional (3D) cases in front of a breakwater. In this study, we focus on the problem near breakwater heads that consists of incident, reflected and diffracted waves. Both wave-induced oscillatory and residual liquefactions will be considered in our new models. The mistake in the previous work [Jeng, D.-S., 1996. Wave-induced liquefaction potential at the tip of a breakwater. Applied Ocean Research 18(5), 229–241] for oscillatory mechanism is corrected, while a new 3D boundary value problem describing residual mechanism is established. A parametric study is conducted to investigate the influences of several wave and soil parameters on wave-induced oscillatory and residual liquefactions around breakwater heads.     

Buckling of multi-segment underwater pressure hull

Results of a numerical and experimental study into buckling performance of multi-segment pressure hull subjected to uniform hydrostatic pressure are discussed. Constituents of multi-segment configurations are bowed-out cylindrical shells with, and without flanges. Details about five collapse tests of laboratory scale mild steel, CNC machined models are given. Segments were about 200 mm diameter, 100 mm long and had uniform wall thickness of 3 mm. Experimental collapse pressures were in the range from 12 to 20 MPa. Numerical collapse pressures agreed well with those obtained during experiments.     

The phase difference effects on 3-D structure of wave pressure acting on a composite breakwater

In designing the coastal structures, the accurate estimation of the wave forces on them is of great importance. In this paper, the influences of the phase difference on wave pressure acting on a composite breakwater installed in the three-dimensional (3-D) wave field are studied numerically. We extend the earlier model [Hur, D.S., Mizutani, N., 2003. Coastal Engineering 47, 329–345] to simulate 3-D wave fields by introducing 3-D Navier–Stokes solver with the Smagorinsky's sub-grid scale (SGS) model. For the validation of the model, the wave field around a 3-D asymmetrical structure installed on a submerged breakwater, in which the complex wave deformations generate, is simulated, and the numerical solutions are compared to the experimental data reported by Hur, Mizutani, Kim [2004. Coastal Engineering (51, 407–420)]. The model is then adopted to investigate 3-D characteristics of wave pressure and force on a caisson of composite breakwater, and the numerical solutions were discussed with respect to the phase difference between harbor and seaward sides induced by the transmitted wave through the rubble mound or the diffraction. The numerical results reveal that wave forces acting on the composite breakwater are significantly different at each cross-section under influence of wave diffraction that is important parameter on 3-D wave interaction with coastal structures.