角叉菜(Chondrus ocellatus Holm)原生质体分离、培养与再生的研究

从角叉菜(Chondrus ocellatus Holm)的孢子体和雌配子体中,分离了大量的成活原生质体。分离的最适条件为:纤维素酶3％,粉红鲍鱼酶8％,山梨醇1.2mol/dm~3,pH6.0,温度为15℃。细胞壁的完全解离,已用荧光增白剂的染色不着色和原生质体在低渗溶液中的易碎性证实。培养后再生的细胞,经均等分裂,随后发育成实心球状体;或出芽后不等分裂,发育成丝状体和愈伤组织状细胞团。在PES培养基中,经30～40d培养,部分球状体和愈伤组织状细胞团分化出完整植株,但丝状体未能分化。低浓度的IAA、6—FA和C—751对球状体和愈伤组织状细胞团的生长和分化都有促进作用。由原生质体培养获得完整的再生植株,这在真红藻纲(Florideophyceae)中为首例报道。     

Numerical studies on lateral load response of fin piles

In the recent past, in order to cater lateral loads, provision of fins to monopiles is evolving as an option for enhancing their lateral load capacity. Numerical and experimental studies pertaining to fin piles subjected to lateral loading are very limited. It is very difficult to understand the behaviour of fin piles through conventional soil pile theories, due to their different fin configurations. In the present study, three-dimensional finite element analyses were performed on regular piles (pile without fins) as well as fin piles. For fin piles, different fin lengths, orientation and position were considered during the analyses. The results have shown that fins placed at the top portion of the piles are more effective than fins placed at bottom of the pile. In all cases, fin piles exhibited more lateral load-carrying capacity compared to regular piles. When fin’s length is less than half of the pile length, lateral load-carrying capacity of fin piles is same irrespective of their orientation. However, fin’s orientation is significant, when fin length is more than 0.5 times length of piles, star fin piles exhibit more lateral load-carrying capacity compared to diagonal and straight fin piles. Fin piles experienced less bending moment compared to regular piles for a given lateral load. Further, it was also noticed that lateral load-carrying capacity of the fin piles depend upon fins’ length and their orientation.