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107 strains producing protease were screened from 260 strains of Antarctic psychrophilic bacteria, among which proteolytic activity of five strains was more than 45 U ml^-1. The 16S rRNA gcne sequences homology and phylogcnetic analysis of five Antarctic psychrophillc bacteria showed that NJ276, NJS-9, NJ16-70,NJ345 belonged tO the described genus Pseudoalteromonas and NJ341 belonged to the genus Colwellia. The growth and the protease characteristic of four Antarctic psychrophilic bacteria had been studied, and the result showed that the 6ptimal temperature for growth and protease-produeing of four strains was about 10℃. Their growth and protease-produeing were still high during incubatlng 2-5 days. The maximum proteolytic activity occurred at pH 9 for four Antarctic psychrophilic bacteria. The optimal temperature of protease action of both strains NJ276 and NJ5-9 was about 50℃, however, the optimal temperature of protease aetlon of both strains NJ341 and NJ345 was about 40 ℃, and their proteolytic activity under 0℃ exhibited nearly 30% of the maximum activity, but their thermal stabilities were weaker. These results indicated that proteases from NJ341 and NJ345 were low-temperature proteases. 相似文献
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随着数字技术的发展,数字信号处理芯片的速度越来越快,这为高速数字滤波的实时实现提供了可能。简要阐述了数字滤波的原理,并对两种数字滤波的实现方法进行了分析;给出了数字滤波较模拟滤波的优势;介绍了数字滤波在射电天文测量中的各种应用。 相似文献
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The search for the progenitors of six core-collapse supernovae (CCSNe) in archival Hubble Space Telescope ( HST ) WFPC2 pre-explosion imaging is presented. These SNe are 1999an, 1999br, 1999ev, 2000ds, 2000ew and 2001B. Post-explosion imaging of the SNe, with the HST ACS/WFC, has been utilized with the technique of differential astrometry to identify the progenitor locations on the pre-explosion imaging. SNe 1999br, 1999ev, 2000ew and 2001B are recovered in late-time imaging, and estimates of the progenitor locations on the pre-explosion imaging, with subpixel accuracy, have been made. Only the progenitor of the Type II-P SN 1999ev has been recovered, on pre-explosion F555W imaging, at a 4.8σ significance level. Assuming a red supergiant progenitor, the pre-explosion observation is consistent with M ZAMS = 15–18 M⊙ . The progenitors of the other five SNe were below the 3σ detection threshold of the pre-explosion observations. The detection thresholds were translated to mass limits for the progenitors by comparison with stellar evolution models. Pre-explosion observations of the peculiarly faint SN 1999br limit the mass of a red supergiant progenitor to M ZAMS < 12 M⊙ . Analysis has been extended, from previous studies, to include possible detections of high- T eff , high-mass stars by conducting synthetic photometry of model Wolf–Rayet star spectra. The mass limits for the Type II-P SNe 1999an and 1999br are consistent with previously determined mass limits for this type of SN. The detection limits for the progenitors of the Type Ibc SNe (2000ds, 2000ew and 2001B) do not permit differentiation between high-mass Wolf–Rayet progenitors or low-mass progenitors in binaries. 相似文献