The DNA de-methylating agent 5-azacytidine does not restore CYP1A induction in PCB resistant Newark Bay killifish (Fundulus heteroclitus)

Newark Bay (NB) killifish (Fundulus heteroclitus) have been chronically exposed to environmental contaminants that activate the aryl hydrocarbon receptor (AHR) and are tolerant to toxic effects and CYP1A induction provoked by AHR ligands. Resistance to CYP1A induction could be due to an epigenetic mechanism such as DNA methylation. We measured in-ovo CYP1A catalytic activity (ethoxyresorufin-O-deethylase, EROD) in NB and reference site killifish embryos aqueously exposed to various concentrations of the de-methylating agent 5-azacytidine, 5-AC (5, 50 and 500 μ(micro)M) with or without 0.2 μ(micro)g/l of the CYP1A inducer 3,3^′,4,4^′,5 pentachlorobiphenyl (IUPAC PCB126). Neither PCB126 alone, nor PCB126 plus 5-AC, induced EROD above levels in vehicle treated Newark Bay fish. In reference site fish, the same PCB126 dose provoked a 7.4-fold EROD induction relative to controls. We conclude that Newark Bay killifish are resistant to CYP1A induction by co-planar PCBs during early embryological development and our data suggests that DNA methylation does not play a critical role in resistance to CYP1A induction in this model.     

一株H_5N_1亚型禽流感病毒株NA基因的克隆与序列分析

用RT-PCR方法从1个H5N1亚型禽流感病毒分离株A/Chicken/Guangdong/DH/1997扩增NA基因cDNA片段,将其克隆至pMD18-T载体,获得重组质粒pMD-NA,并对其核苷酸序列进行测定和分析。结果表明,该毒株的NA基因长度为1350bp,编码449个氨基酸,与其它H5N1亚型AIV分离株的核苷酸序列同源性为97.0%～99.4%,氨基酸序列同源性为97.7%～99.1%,提示禽流感病毒NA基因保守性较高。NA基因氨基酸序列的聚类分析表明该毒株与来自香港的A/Pheasant/HK/FY155/01和A/Ch/HK/FY150/01两个分离株处于同一进化枝,亲缘关系较近。     

Detecting Arctic snow and ice cover with FY-1D global data

Chinese meteorological satellite FY-1D can obtain global data from four spectral channels which include visible channel(0.58-0.68 μm) and infrared channels(0.84-0.89 μm,10.3-11.3 μm,11.5-12.5 μm).2366 snow and ice samples,2024 cloud samples,1602 land samples and 1648 water samples were selected randomly from Arctic imageries.Land and water can be detected by spectral features.Snow-ice and cloud can be classified by textural features.The classifier is Bayes classifier.By synthesizing five d ays classifying result of Arctic snow and ice cover area,complete Arctic snow and ice cover area can be obtained.The result agrees with NOAA/NESDIS IMS products up to 70%.     

考虑关断时间效应的瞬变电磁一维反演

为克服因关断时间校正而带来的误差,尝试直接对包含关断时间效应的瞬变电磁资料进行反演。正演采用将斜阶跃波离散为多个阶跃波之和的方法,反演采用经典的马奎特法。对模型试算的结果表明,采用该方法直接对斜阶跃响应进行反演,结果比较理想,计算速度也可以接受。     

Trophic modeling of the Northern Humboldt Current Ecosystem, Part II: Elucidating ecosystem dynamics from 1995 to 2004 with a focus on the impact of ENSO

The Northern Humboldt Current Ecosystem is one of the most productive in the world in terms of fish production. Its location near to the equator permits strong upwelling under relatively low winds, thus creating optimal conditions for the development of plankton communities. These communities ultimately support abundant populations of grazing fish such as the Peruvian anchoveta, Engraulis ringens. The ecosystem is also subject to strong inter-annual environmental variability associated with the El Ni1;o Southern Oscillation (ENSO), which has major effects on nutrient structure, primary production, and higher trophic levels. Here our objective is to model the contributions of several external drivers (i.e. reconstructed phytoplankton changes, fish immigration, and fishing rate) and internal control mechanisms (i.e. predator-prey) to ecosystem dynamics over an ENSO cycle. Steady-state models and time-series data from the Instituto del Mar del Perú (IMARPE) from 1995 to 2004 provide the base data for simulations conducted with the program Ecopath with Ecosim. In simulations all three external drivers contribute to ecosystem dynamics. Changes in phytoplankton quantity and composition (i.e. contribution of diatoms and dino- and silicoflagellates), as affected by upwelling intensity, were important in dynamics of the El Ni1;o of 1997 13;98 and the subsequent 3 years. The expansion and immigration of mesopelagic fish populations during El Ni1;o was important for dynamics in following years. Fishing rate changes were the most important of the three external drivers tested, helping to explain observed dynamics throughout the modeled period, and particularly during the post-El Ni1;o period. Internal control settings show a mix of predator 13;prey control settings; however a 1c;wasp-waist 1d; control of the ecosystem by small pelagic fish is not supported.     

基于GM(1,1)优化模型的岩石边坡变形预报

岩石边坡系统是一典型灰色系统，其变形发展过程可以用灰色预测模型完成。尽管传统GM（1，1）模型预测有很多成功的实例，但是也存在一些预测偏差过大的情况，必须对其进行优化。逐步迭代法GM（1，1）模型不仅收敛速度快，而且与原始数据库序列的凹凸性保护一致。利用自编的计算程序对马步坎边坡预测测点G1沉降和开裂进行预测分析，结果表明逐步迭代法GM（1，1）优化模型计算精度较传统GM（1，1）模型和背景构造法GM（1，1）优化模型高，较好地反映了岩石边坡的变形趋势。     

荔湾3-1外输海底管道中落管抛石技术

落管抛石是进行深水抛石的一种工程技术,可作为保证管道稳定性、处理悬跨、抑制隆起屈曲和进行跨越支撑的工程解决方案。系统研究了抛石在波流作用下的稳定性,落石对管道冲击的影响以及施工技术。针对南海环境条件下的抛石稳定理论进行了修正;分析了三层聚丙烯涂层管道在落石冲击下的可接受抛石粒径;提出同时结合石块稳定性结果和管道抗冲击性能要求下的石料分级的方法;最后,对该技术在荔湾3-1外输海底管道的工程应用进行详细阐述。     

灰色系统理论预测大气氮氧化物污染的应用

根据中国南方某省所辖城市近年来N0x污染指数的测量数据的统计资料，建立了GM(1，1）灰色预测模型，通过预测探讨了大气中氮氧化物指数的变化趋势。所建模型残差仅1．17％，关联系数为0．9067，精度较高，具有较好的可行性和适应性，可以为管理规则提供决策依据。     

注射鳖源致病性嗜水气单胞菌ZHYYZ-1引发中华鳖(Trionyx sinensis)稚鳖感染和致死的定量研究

以20日龄中华鳖稚鳖为实验动物,采用静水停食实验法,在水温(27.4±1.3)℃、pH7.1条件下,进行了注射鳖源嗜水气单胞菌ZHYYZ-1引发中华鳖稚鳖感染和致死的定量实验研究。结果表明:(1)该菌为高致病性菌株,异常排泄是该菌致稚鳖表露临床感染症状的重要标志;(2)注射该菌引起稚鳖感染与致死具明显的剂量—时间效应,随注射菌量递增依次出现不表露临床感染症状、表露临床感染症状而不出现死亡和大量死亡三种情形,稚鳖表露临床感染和出现死亡的时间均随注射菌量增大而明显缩短,绝大多数稚鳖表露临床感染和出现死亡的时限分别为2h和48h;(3)濒死稚鳖体内该菌的底限含量为3.3×106CFU/g,稚鳖体内菌注射含量可控安全范围为(1.2—8.8)×103CFU/g。