舟山潮间带生态学研究：Ⅱ.数量及其分布

舟山潮间带生物种类数量的平面分布与盐度和海岸开敞性有关,垂直分布与潮汐有关;群落数量的水平分布与底质和海岸开敞性有关,垂直分布与潮汐有关。舟山潮间带生物有明显的分带现象,隐蔽的岩岸区分为:滨螺带和藤壶-牡蛎带两个带;开敞性岩岸区分为:滨螺带,藤壶-(虫戚)-藻类带及藻类带3个带。沙滩区分为:沙蟹带和浅蛤-斧蛤带两个带;泥沙滩区分为:盐碱植物-滨螺带和招潮蟹-大眼蟹带;泥滩区分为:蟹守螺-囊螺带和蟹守螺-织纹螺带。     

大型多元统计软件PRIMER的方法原理及其在底栖群落生态学中的应用

本文阐述大型多元统计分析软件 PRIMER的原理及其在底栖生态学中的应用。PRIMER是处理生态和环境多元数据的重要数值分析工具 ,本文从实用的角度对其中包括的技术方法和相应的子程序如 :等级聚类 (CLUSTER)、非度量多维标度 (MDS)、主分量分析 (PCA)、相似性分析检验(ANOSIM)、相似性百分比分析 (SIMPER)、生物 -环境连接的逐步分析和相关检验 (BIOENV/BVSTEP)等作了较为系统的介绍。     

象山港大型底栖动物群落特征

2006年7月—2008年8月,对象山港全港海域设立的13个采样站位采集的调查资料,用物种丰富度指数、物种均匀度指数和物种多样性指数分析象山港大型底栖动物物种的多样性和群落种类的组成,以及采用ABC曲线方法和大型多元统计分析软件PRIMER5对象山港大型底栖动物进行Bray-Curtis相似性聚类分析和非度量MDS标序,研究象山港大型底栖动物群落结构。调查显示象山港大型底栖动物不同采样站位之间物种丰富度指数(d)、均匀度指数(J)、辛普森多样性指数(D)和香农-威纳多样性指数(H’)差异皆高度显著(P0.01)。群落结构聚类分析和MDS标序表明,13个采样站位的大型底栖动物群落大致可分为3组。根据所调查象山港大型底栖动物的丰度和生物量资料做的ABC曲线分析表明,该海域大型底栖动物群落受到了严重的环境污染或者扰动,逐渐由一种或几种个体较小的种类占优势。     

江苏海岸带景观及其生态旅游的开发

海岸带是陆地、海洋生态系统相互作用的生态过渡地带,也是人类赖以生存的非常独特的景观资源。由于人口增长和对资源的不合理开发,海岸带环境遭到破坏而面临着资源枯竭的危险,保护海岸带景观异质性和生态整体性对海岸带的可持续利用显得尤为重要。江苏海岸带异质景观的形成是受自然驱动要素和干扰共同作用的结果,其影响因素主要包括江苏海岸地貌演化、气候水文条件、土壤植被条件等自然要素和台风暴潮灾害、米草引种和人类开发活动等干扰。江苏海岸带景观具有结构的复杂性、功能的多样性、生态的脆弱性、环境质量较高和高、低对比度兼有等特征。从风景地貌学和旅游开发角度考虑,将江苏海岸带景观划分为北部山海景观类、中部滩涂景观类和南部河口景观类,并在景观类的基础上进一步划分出景观亚类和景观型两个层次。提出了加强景观生态建设,维持景观的异质性,建设射阳河—新洋港流域的荷兰式国家低地生态公园和盐城国家海洋湿地生态公园,适度开发沿海自然保护区景观生态旅游,打造北部山海景观生态旅游特色项目,深入开发南部江海交汇景观,挖掘海岸带旅游景观资源的文化异质性,以及启动辐射沙洲海洋世界自然遗产的申报和建设8项生态旅游规划。     

北部湾白龙半岛邻近海域污损生物生态研究

2006年3月至2007年2月在北部湾白龙半岛邻近海域进行周年污损生物挂板试验, 并调查该海域码头和养殖网箱的污损生物状况.调查结果表明: 该海域污损生物群落呈现明显的亚热带港湾污损生物生态特点.调查记录污损生物共82种, 其中优势种为网纹藤壶Balanus reticulatus、有孔右旋虫Dexiosprira foraminosus、左旋虫Spirorbis papillatus、多室草苔虫Bugula neritina、大室膜孔苔虫Membranipora grandicella、美丽琥珀苔虫Electra tenella、缘齿牡蛎Dendostrea crenulifera、冠瘤海鞘Styela canopus和翡翠贻贝Perna viridis.生物全年附着, 2006年5-10月为附着高峰期.北部湾白龙邻近海域的月板、季板和年板平均附着生物量分别为200.54、2 550.37和15 121.67g·m-2, 均比大亚湾和湛江港的低, 而比清澜港和北部湾涠州岛的高.码头附着生物呈明显的垂直分带现象: 高潮带为滨螺区, 中潮带为牡蛎-笠藤壶分布带, 低潮带主要为藻类.在调查的基础上, 还针对海洋工程、海水养殖和河口生态环境保护给出不同的防污建议.     

Methods Comparison for Microsatellite Marker Development： Different Isolation Methods, Different Yield Efficiency

Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology, quantitative genetics and genomics. Therefore, it is extremely necessary to select several versatile, low-cost, efficient and time- and labor-saving methods to develop a large panel of microsatellite markers. In this study, we used Zhikong scallop (Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency, while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time- and cost-saving, it is difficult to obtain a large number of microsatellite markers, mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study, we recommend two methods, microsatellite-enriched library construction method and FIASCO-colony hybridization method, for large-scale microsatellite marker development. Both methods were derived from the mi-crosatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes.     

山东南部沿海冬季小型底栖生物的初步研究

利用统计法、经验模型和多元聚类分析法和数学分析软件对2006年12月908项目冬季航次山东南部海域(36°15′—36°45′N,120°45′—122°00′E)小型底栖生物调查所获得的数据进行分析,获得该调查海域冬季小型底栖生物的平均丰度为(657.43±214.60)ind/10cm2,平均生物量为(847.15±427.63)μgdwt/10cm2,平均生产量为(7624.32±3848.64)μgdwt/(10cm2·a-1);还对N/C、Simpson优势度指数、Margalef多样性指数、Pielou均匀度指数和香农-威纳指数进行了计算。与2006年8月908项目夏季航次同一海域调查数据进行了比较,得到小型底栖生物的平均丰度及其优势类群线虫丰度冬季明显低于夏季,而生物量和类群多样性与夏季相当。同时,依据丰度指标对山东南部研究海域进行了初步的生态学划分,呈现出较明显的地域性。     

A landscape pattern analysis method based on boundaries and nodes: A case study in upper Minjiang River, China

Traditionally,patch-based analysis at the landscape scale fragmentation has been used in the study of landscape ecology while the study of boundary and node has not been considered as much detail until recently.This study investigated the possibility of applying boundary-and node-based methods in landscape pattern analysis to the upper reaches of the Minjiang River in the southwestern China.Boundary-based and node-based landscape indices were selected to be used in analyzing changes in landscape patterns,and the results were compared with analysis using traditional pattern indices.We compared the responses of patch-area-based,boundary-length-based and node-number-based indices,and concluded that boundary-based and node-based indices are more sensitive to disturbance than patch-based indices with various patterns,and node-based indices are even more sensitive than boundary-based ones.Thus,the results suggest that boundary-based and node-based pattern analysis methods provide helpful supplementary information to traditional patch-based pattern analysis methods.The results about pattern dynamics of landscapes in the upper reaches of the Minjiang River based on boundaries and nodes showed that with human disturbance,the dominance of forest landscape was weakened by other landscape types; thus the landscape pattern of the study area became more homogeneous and the boundary network became more complex.These changes further augmented disturbance interfaces in the landscape and increased the possibilities of further landscape fragmentation.     

论人口容量及其研究方法

在前人研究成果的基础上，从人类生态学角度出发，辨析人口容量的涵义，探讨了人口容量的特征，概述人口容量的研究方法。