石斑鱼虹彩病毒SGIV在宿主细胞内依赖微管运动的行为特征

病毒是非常微小的简单生物, 不能独立生存, 必须借助宿主细胞完成自身的繁衍。病毒侵染进入细胞后, 通常借助于微管通过黏稠的细胞质运动到特定的复制位点。然而, 有关病毒依赖微管运动行为的精细动态研究还比较少。石斑鱼虹彩病毒(Singapore grouper iridovirus, SGIV)为虹彩病毒科蛙病毒属的一个新种, 是海水养殖鱼类的重要病毒性病原, 对海水养殖业造成重大经济损失。利用单粒子示踪技术实时追踪了SGIV病毒粒子沿微管运动的行为, 观察到SGIV在细胞边缘至微管中心之间的双向运动, 最高瞬时速度约0.2μm·s^-1, 均表现为主动运输。病毒粒子运动至微管交叉位置会减速迂回, 而后或受限于此, 平均运动速率约0.008μm·s^-1, 或通过交叉处继续快速运动, 最高瞬时速度为0.2μm·s^-1。同时, SGIV感染会影响微管的形态结构, 随着SGIV感染, 微管逐渐围绕细胞核和病毒加工厂形成环状结构。研究结果初步揭示了SGIV病毒和细胞微管之间相互作用的复杂过程, 丰富了我们对虹彩病毒胞内生命活动的认识, 有助于深入地理解海水鱼类虹彩病毒感染致病机理。     

红河断裂构造带在东南亚的延伸特征及其大地构造意义

根据地质、地貌及地球物理资料分析,探讨展布于云南边陲哀牢山两侧的北西向断裂组成的红河断裂构造带,在东南亚的延伸特征。提出在该延伸带两侧沉积建造、构造活动,地球物理场和大地构造发展上均有显著差异。并进一步探讨该断裂构造带在大地构造上的意义。     

Genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences

The fragments of 350 bp in 28S rRNA from the closely related monogenea of trematoda, Neobenedenia girellae and N. melleni are obtained by polymerase chain reaction (PCR) amplified using a couple of special primers and then sequenced. The results show that the comparison of 28S rRNA sequences, with only a base varying in 337 bp accounting for 0.3% genetic difference, from the relative species N. girellae and N. melleni parasitized on the different fishes in different farms displays that they possess a very high genetic similarity of 99.7%, higher than that of 99.41% for the single species N. melleni sampled in different areas, and the intraspecific divergence of N.melleni is 0.59%. Meanwhile, the interspecific differences between the two Neobenedenia and three Benedenia (i.e., B. lutjani, B. rohdei and B. seriolae) range from 2.08% tol 1.73%. In addition, UPGMA and MP molecular phylogenetic trees are constructed and proved to be consistent with each other. Though the morphological characteristics and the results of genetic diversity for the two Neobenedenia show a high similarity, whether they belong to a single species or not are still undefined, and the more genes of them should be further investigated, in combination with the systematical and detailed morphological study.     

往复流中双向导向叶冲动透平模型性能试验研究

研制了 30 0双向自调导叶透平试验机组 ,安装在 10 0 0活塞式往复流透平试验台上 ,对两种叶轮方案和 6种不同喷咀、扩压器进行了不同活塞行程、不同周期和不同恒定输出电压下性能试验。试验结果表明在喷咀出口角α1=2 1 5°、扩压器入口角α2 =6 5 5°和α1=2 4°、α2 =6 3°组合最佳 ,最高效率达 5 3 3% ,而且在宽广流量系数范围内 ,特别是大流量系数范围内都有较高效率。大大优于常用对称翼透平。将上下游导叶固定 ,变成双向固定导叶透平。用No 1叶轮和 6种导叶出口角组合进行试验。表明最佳导叶出口角为 2 4°～ 30°,最高效率仍达 38 5 % ,比对称翼透平仍高许多     

The Role of Fracture Structure in Leachate Pollution at Landfills: A Case Study of a Landfill in Chongqing City

This paper studies a landfill where there are three faults running through. As serious pollution has occurred to the geological environment, the landfill is to be closed up and renovated. The paper aims to explore the role of fracture structure in leachate pollution at the landfill. The research was carried out in several stages. First, mathematical models of the pre-renovation landfill with three faults running through and the landfill after renovation were established. And then, the boundary conditions and parameters of the two mathematical models were determined. The groundwater level of the landfills was simulated in order to modify the two mathematical models. As a result, a feasible mathematical model was achieved. Based on this model, a comparison was made of the COD concentration variations in the inside leachate and outside leachate between the two landfills. Accordingly, the impacts of the fracture structure on the pollution of leachate at the landfills could be identified. The study results show that while faults contribute to the migration of ieachate, they also serve as a confluence of leachate, thus further deteriorating the environment. The COD concentrations of the inside leachate and the outside leachate of the pre-renovation landfill are respectively 800 mg/L and 220 mg/L higher than those of the post-renovation landfill. Therefore, measures must be taken to handle the ieachate seepage in areas where there are faults as well as the neighboring areas so as to get the environmental pollution under control.     

祁阳山字型构造质疑

结合区域构造演化背景及构造变形特征,对祁阳弧形构造的形成机制进行研究。分析表明,传统山字型构造机制难以解释祁阳弧形构造的诸多特征,且前人认为变形动力来源于东西向挤压应力场,也与印支期区域构造应力场实为NWW向的构造背景不符,因此祁阳弧形构造并不属典型山字型构造。基于研究区构造活动的客观实际,提出祁阳弧形构造的可能形成机制:NW向基底隐伏断裂和NNE-NE向主干断裂分别于印支期和燕山期产生强烈左旋走滑活动,从而使区域NNE向构造线在中段产生左旋偏转成为NNW向,从而形成S形的祁阳弧形构造。这一机制可较好解释祁阳弧形构造的若干特征,如关帝庙穹窿呈NWW走向、北反射弧构造形迹不显著、北弧弧顶脱位及内弧曲率大于外弧、南弧弧顶脱位、紫云-中田-高峰串珠状穹窿的形成等。上述认识对华南地区弧形构造研究具有一定启示意义:除山字型构造作用和砥柱作用外,还应注意断裂走滑等其他构造活动对弧形构造的制约,以及多次构造活动叠加对构造形迹可能造成的影响。     

四参数速度分析方法在新场转换波处理中的应用

转换波时距方程的选择直接影响转换波速度分析和动校的准确度,这里比较了Thoms-en方程、双平方根方程和LXY方程的动校精度.根据新场气田转换波资料的特点,采用四参数(V,c2>、γ,0、γ,eff和X,eff)各向异性速度分析方法来估计叠加速度场.在速度分析过程中,不依赖于纵波速度,直接进行三参数估计.在转换波动校时,不同参数控制不同偏移距范围的同相轴,使转换波近、中和远偏移距同相轴完全拉平.通过对实际测线的单参数、三参数和四参数动校叠加剖面的比较,结果表明四参数动校叠加的效果最好.     

基于GPS数据的浑河断裂带抚顺市区段现今活动性定量分析

浑河断裂带是深大断裂-郯庐断裂的北延分支,横贯抚顺市区,在断裂带之上及其两侧建有诸多大型工矿企业及民用建筑,断裂带的稳定与否关系到抚顺市的城市安全。近期,浑河断裂带抚顺市区段的个别次级断裂发生活动,并已造成严重的城市建筑破坏。局部断裂活动的成因,为城市决策者所关注。本文利用抚顺市城市平面控制网相隔17a的两期GPS测量数据,应用大地形变分析的理论方法,对浑河断裂带抚顺市区段的稳定性进行了定量分析,得出结论:浑河断裂带抚顺市区段的宏观地质构造在此期间活动性不明显。     

基于GPS的四川地区现时地壳形变分析

对四川区域网多年数据进行处理,得到欧亚框架下的测站运动速度,采用刚性地块假设下的最小二乘拟合方法,得到川滇、川青、扬子地块的运动速度分别为(17.6±1.0)mm/a、(11.4±0.7)mm/a、(9.6±1.1)mm/a,地块运动方向逐渐由SE向SEE变化;作为川滇地块东边界的鲜水河断裂、安宁河-则木河断裂运动速度远大于龙门山断裂,显示了其较强的活动性。     

高精度重磁测量在商都地区CO2气藏勘查中的应用

通过在商都地区开展高精度重磁测量,提高了对盆地基底结构、构造、断裂特征与断裂分布以及汉诺坝组玄武岩分布的认识.商都坳陷具有单断式的结构特征,即西断东超.坳陷走向为北西向,由西北、东南两个次级洼槽组成;区内分布有两条北西南东向展布的深大断裂,为幔源CO2的运移通道,控制着CO2气藏的分布;局部强磁性、高密度异常为汉诺坝玄武岩的反映,是小丹岱组CO2气藏的良好盖层,控制着小丹岱组CO2气藏的分布.