Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.     

Issues concerning global warming today

The global weather of today is growing significantly warmer; this is an indisputable fact. However, the scientific community has not yet reached consensus on the causes of global warming and its possible consequences. This paper introduces the causes of global warming and summarizes its results, which both involve a series of huge and complex system issues. Our top priority is to pinpoint the main reason and the interrelated links between causative factors by adopting a macro-approach, or comprehensive comparison analysis. Its physical mechanism was then determined and its digital model established after quantitative study. __________ Translated from Green Leaf, 2007, (8): 34–35 [译自: 绿叶]     

四川盆地碳酸盐岩油气勘探实践与认识

四川盆地烃源十分丰富。但油气演化程度高,大多已进入高成熟和过成熟阶段,至今只在侏罗系发现有油藏,三叠系及其更老地层皆为气藏。储层主要为碳酸盐岩,其特点是低孔低渗,平均孔隙度仅1.7%,平均渗透率小于1×10~(-3)μm~2,但其中的“孔洞发育层段”平均孔隙度可达3%～6%。根据四十年的勘探经验,加强预探并争取新发现是四川盆地油气勘探发展的关键。预探中,按构造带规划大中型气田勘探目标,将成藏条件相似、地域上相邻的局部圈闭群作为一个整体勘探对象,实行稀井广探,少井高效。“八五”以来,在高陡构造带勘探技术、储层横向预探技术等方面有长足的进步,勘探成效不断提高,实现了资源序列的良性循环。     

多相地貌单元场地勘察中基岩与卵砾石、孤石的鉴别

在多相地貌单元场地勘察中，基岩与卵砾石的鉴别难度较大。应利用初步感性认识、钻进状态、岩样特征等多手段对基岩与卵砾石进行判别，以提高判别率。     

利用催化动力学原理测定化探样中微量碘的含量

在HAc-NaAc形成的中性缓冲溶液体系中,利用碘的催化动力学原理,使N-N-四甲基-4-4-二氨基二苯甲烷与氯胺T的氧化反应,生成蓝色络合物来测定地探样中微量碘的含量。通过实验,最佳试剂加入量为8%的HAc溶液5mL,8%的Na2CO3溶液3mL,0.1%的N-N-四甲基-4-4-二氨基二苯甲烷溶液5mL所组成的溶液体系中,在加入0.1%的氯胺T1mL显色后,其测定结果准确度、精密度高,检出限低,测定化探样中微量碘时该方法可以推广应用。     

卡尔曼滤波相位解缠及其与其他方法的对比分析

一般相位解缠算法通常在相位解缠前首先需要借助滤波算法进行噪声消除,随后才能相位解缠,以保证解缠的质量和顺利实施。卡尔曼滤波将相位解缠问题转化为状态估计问题,实现相位解缠与噪声消除一并处理,简化了数据处理过程。利用真实干涉SAR数据进行实验,采用卡尔曼滤波相位解缠算法进行处理,并与其他几种较常用相位解缠算法的结果从目视和定量两方面进行比较和分析。验证了卡尔曼滤波相位解缠方法在滤波和解缠效果方面的有效性和可行性,可以获得较为可靠的解缠结果。     

灵武市体育中心沉降监测分析

根据相关规范,对灵武市体育中心建设过程进行沉降监测,通过监测结果得到沉降量和沉降速率随时间的变化趋势并进行预测分析,得到该建筑物的沉降变形规律,用于了解工程安全情况。     

测绘资料档案管理及应用探析

介绍了河南省建国以来测绘成果资料的保管、分发与应用工作,讲述了测绘成果应用在国民经济建设中取得的成就。     

春晓气田泥岩垮塌测井数据校正及应用

由于存在部分泥岩井眼垮塌,东海春晓气田测井曲线砂泥岩阻抗部分叠置。针对这一现象,采用未垮塌段泥岩伽马与密度拟合关系校正垮塌段泥岩密度,声波时差经本区伽德那公式拟合后求取。通过泥岩垮塌测井数据校正前后对比、直方交会、岩石物理、正演及反演效果分析,认为校正后的测井数据比较真实地反映垮塌段扩径泥岩地层特征,储层与围岩波阻抗差异明显,为储层反演、砂体空间展布分析提供了有力依据。     

遥测降雨抗差分析方法应用研究

遥测系统观测的雨量资料常会包含粗差，根据降雨量的特征，结合抗差理论，包为民等曾提出三步抗差修正方法。将此法应用到龙颈上水库等10个水库流域的实测资料当中，验证其有效性，并分析比较各步抗差修正的效果及降雨特征与抗差效果之间的关系。结果表明，抗差修正方法可行，且第一步和第三步结合的修正效果最好；降雨中心较明确，多年降雨特征相似，强度变幅稳定的洪水，不容易出现误抗。