杆状病毒感染越冬亲虾(Penaeus chenesis)的研究——越冬亲虾感染及其垂直传播的可能性

在中国对虾越冬亲虾中检出杆状病毒。经对病虾鳃、前中肠、卵巢组织进行电镜观察，在这些组织中发现大量有囊膜的杆状病毒。病毒粒子大小约为３６７ｎｍ×１２８ｎｍ，核衣壳为３２４ｎｍ×９２ｎｍ。病毒在细胞内不形成包涵体，在核内或在细胞质的发生基质中装配。从病毒的形态特征、感染组织以及典型的发病症状和病理变化，初步认为亲虾感染的病毒与近年广泛报道的造成养殖对虾大规模流行病的主要原因——中国对虾杆状病毒为同一种。卵巢组织也是病毒的主要靶器官之一，亲虾卵巢组织的被感染暗示着该病毒有可能通过生殖细胞而垂直传播     

Three-dimensional numerical simulation of obfique wave action on vertical walls

A three-dimensional numerical model based on the potential theory was developed to study the oblique wave action on vertical walls. A source term inside the domain was used to generate incident waves and outgoing waves were dissipated by sponge layers and transmitted by radiation boundaries. The finite difference method was used to solve the governing equations and boundary conditions in the regular transformed domain in σ-coordinate. Satisfactory agreements between the numerical predictions and experimental results of wave force were obtained. It is concluded that the maximum wave force acting on the vertical walls is induced by the obliquely incident waves rather than the normally incident waves.     

Elucidating the dynamics and mixing agents of a shallow fjord through age tracer modelling

The mixing agents and their role in the dynamics of a shallow fjord are elucidated through an Eulerian implementation of artificial tracers in a three-dimensional hydrodynamic model. The time scales of vertical mixing in this shallow estuary are short, and the artificial tracers are utilized in order to reveal information not detectable in the temperature or salinity fields. The fjord's response to external forcing is investigated through a series of model experiments in which we quantify vertical mixing, transport time scales of fresh water runoff and estuarine circulation in relation to external forcing.Using age tracers released at surface and bottom, we quantify the time scales of downward mixing of surface water and upward mixing of bottom water. Wind is shown to be the major agent for vertical mixing at nearly all depth levels in the fjord, whereas the tide or external sea level forcing is a minor agent and only occasionally more important just close to the bottom. The time scale of vertical mixing of surface water to the bottom or ventilation time scale of bottom water is estimated to be in the range 0.7 h to 9.0 days, with an average age of 2.7 days for the year 2004.The fjord receives fresh water from two streams entering the innermost part of the fjord, and the distribution and age of this water are studied using both ageing and conservative tracers. The salinity variations outside this fjord are large, and in contrast to the salinity, the artificial tracers provide a straight forward analysis of river water content. The ageing tracer is used to estimate transport time scales of river water (i.e. the time elapsed since the water left the river mouth). In May 2004, the typical age of river water leaving the fjord mouth is 5 days. As the major vertical mixing agent is wind, it controls the estuarine circulation and export of river water. When the wind stress is set to zero, the vertical mixing is reduced and the vertical salinity stratification is increased, and the river water can be effectively exported out of the fjord.We also analyse the river tracer fields and salinity field in relation to along estuary winds in order to detect signs of wind-induced straining of the along estuary density gradient. We find that events of down estuary winds are primarily associated with a reduced along estuary salinity gradient due to increased surface salinity in the innermost part of the fjord, and with an overall decrease in vertical stratification and river water content at the surface. Thus, our results show no apparent signs of wind-induced straining in this shallow fjord but instead they indicate increased levels of vertical mixing or upwelling during down estuary wind events.     

Temporal change of clustered distribution of planktonic ciliates in Toyama Bay in summers of 1989 and 1990

Temporal change of clustered distribution in vertical profiles of three nutritional groups of planktonic ciliates, e.g. heterotrophic naked ciliates, mixotrophic naked ciliates and heterotrophic loricated ciliates, was investigated by following a drifting buoy in Toyama Bay on the Japan Sea coast of central Japan in summers of 1989 and 1990. Clustered distribution, represented as the mode of population density in the vertical plane, occurred mainly in the oligotrophic upper layer (0–50 m depth) above the subsurface chlorophyll-a maximum layer. Its clustered degree was stronger when the mode of population density in the vertical plane was formed at shallower depth, while its longevity was shorter as mentioned above. Vertical distribution of ciliates during summer in Toyama Bay is characterized by ephemeral clustered distribution, or in other wards, by rapid alternations of appearance and disappearance of the clustered distribution.     

湍流数值模拟中封闭模式应用的局限性

通过对琼州海峡的潮流场特征进行数值模拟，指出了选择不同的特征混合长度表达式对数值模拟结果的影响，表明了基于特征混合长度理论的流封闭模式在近海湍流数值模拟中应用的局限性。     

长江口拦门沙地区的泥沙运动规律

依据１９８８年７月和１２月水文观测资料，通过流，盐，沙的综合分析，认识到长江口拦门沙地区的泥沙运动有与河口其他地区不同的特点，泥沙输移在水平面上和垂直面上存在着多种循环，潮流和盐水截留大量泥沙积聚的拦门沙地区，河口在盐淡水交锋地带，除了涨，落急时段外，发现在转流时期泥沙也可能产生再悬浮，从而形成一个潮周期中出现３次或４次再悬浮的特殊规律。     

Experimental studies on the slowly varying drift motion of a berthed container ship model

The effects of free-surface waves on the floating structures are of great importance in the offshore industry. Among the six degrees of motions of a surface ship the absence of restoring forces in surge, sway and yaw led to critical situations for moored ships in the recent times. The order of forces in horizontal plane and their exciting frequencies are matters of interest. The resonance with the presence of moored chains led to many accidents in the recent past. The lines in dry conditions may not give good damping and in wet condition they may trigger the system to chaotic motions and jumps. Two different loading conditions of a container ship model are tested with waves in laboratory conditions in two different drafts. The mooring lines are chosen as per scale law and the energy under the response spectrum is determined from the plots. The results give new insights into the movement of a berthed ships subjected to waves. Response of the moored ship to different loading conditions in different water depths are discussed in this paper. The paper gives the order of energy due to first-order and slowly varying movement of a berthed container model in a towing tank.     

Microphytobenthos vertical migratory photoresponse as characterised by light-response curves of surface biomass

The migratory response of intertidal microphytobenthos to changes in irradiance was studied on undisturbed estuarine sediments. Two non-destructive optical techniques were used to trace variations in vivo of surface biomass: PAM fluorometry, for measuring the minimum fluorescence level (F_o); and spectral reflectance analysis, for quantifying the normalized difference vegetation index (NDVI). Following the formation of a dense biofilm at the surface, replicated sediment samples were simultaneously exposed to six different irradiance levels, ranging from 50 to 1500 μmol m⁻² s⁻¹, during a period of 120 min. The migratory photoresponse of the biofilms was characterised by constructing biomass vs. light curves (BLC), relating the accumulation of microalgal biomass after that period (estimated by F_o or NDVI) to the irradiance level incident on the surface. BLCs allow characterising the main features of the migratory photoresponse of intact biofilms. Typical BLC showed a clear biphasic pattern, with an increase in microalgal accumulation under irradiances below 100 μmol m⁻² s⁻¹, maximum values under 100–250 μmol m⁻² s⁻¹, and a gradual decrease of surface biomass under higher irradiances, indicating a strong photophobic downward migratory response. Similar BLC patterns were obtained when measuring F_o or NDVI. The construction of BLCs for biofilms from intertidal sites with distinctive sediment characteristics and diatom taxonomic composition allowed to detected significant differences in the migratory photoresponse. Biofilms from a muddy sediment exhibited considerably larger amplitude in the migratory photoresponse than the biofilms from a sandy mud site, especially under high irradiances. The photophobic migratory response to high light was found to vary among diatom species, particularly in the case of the biofilms from the muddy sediments.     

Vertical distribution of the macrobenthic community and its relationships to trace metals and natural sediment characteristics in the lower Douro estuary, Portugal

Vertical distribution (0–15 cm) of the macrobenthic community and its relationships to natural sediment characteristics and trace metal contents and bioavailability were studied at five locations in the lower Douro estuary, Portugal. An analysis of vertical metal distribution, for the interpretation of anthropogenic impact on the estuarine sediments, was also investigated. Sediment characterisation included organic matter, grain size, metals (Al, Fe, Cu, Pb, Cr, Ni, Cd, Zn and Mn), acid volatile sulphide (AVS) and simultaneously extracted metals (SEM). The macrobenthic community had low diversity (14 species), was dominated by small size opportunists and seemed to be controlled mainly by natural factors such as grain size distribution, Al and Fe contents and sediment depth. The vertically heterogeneous distribution of macrobenthic community appears to affect redox status of the sediments and consequently metal bioavailability. Despite anthropogenic contamination in terms of Zn, Cu, Pb, Cr and Ni having already been detected in the north bank, the analysis of vertical distribution was essential for the identification of current anthropogenic contamination in terms of Zn, Pb and Cd in the south bank.