Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis)

A new cell line,CSEC,has been successfully established from embryos at gastrula stage of a cultured marine fish,half smooth tongue sole(Cynoglossus semilaevis).CSEC cells grow actively and stably more than 50 passages for over 200 d in DMEM medium supplemented with 15% FBS(fetal bovine serum),2.5 ng/cm 3 bFGF(basic fibroblast growth factor),1 ng/cm 3 LIF(leukemia inhibitory factor) and 50 mmol/dm 3 2-ME(2-mecaptoethanol).The cells grew well in the temperature range of 24-30 ℃ and the optimal growth temperature was 24℃.FBS and bFGF concentrations are the two key components for CSEC cells proliferation.Chromosome analysis reveals that CSEC cells have a normal diploid karyotype with 2n=42t.The significant fluorescent signals were observed in CSEC after transfection with the GFP reporter gene,suggesting that the CSEC cell line can be used as a useful tool for transgenic and genetic manipulation studies.CSEC cells showed the cytopathic effect(CPE) after infection with lymphosystis disease virus(LCDV) in 2 d.Moreover,the LCDV particles can be observed in the cytoplasm of virus-infected cells by electron microscopy.It suggests that CSEC could be potentially used for the study of aquatic virus.     

Effect of slow-solvent-vapour treatment on performance of polymer photovoltaic devices

In this work, enhanced poly(3-hexylthiophene):[6,6]-phenyl-C61-butyric acid methyl ester (P3HT:PCBM) bulk-heterojunction photovoltaic devices are achieved via slow-solvent-vapour treatment. The correlations between the morphology of the active layer and the photovoltaic performance of polymer-based solar cell are investigated. The active layers are characterized by atomic force microscopy and optical absorption. The results show that slow-solvent-vapour treatment can induce P3HT self-organization into an ordered structure, leading to the enhanced absorption and efficient charge transport.     

Stable 85Rb micro vapour cells: fabrication based on anodic bonding and application in chip-scale atomic clocks

We describe the microfabrication of ⁸⁵Rb vapour cells using a glass-silicon anodic bonding technique and in situ chemical reaction between rubidium chloride and barium azide to produce Rb. Under controlled conditions, the pure metallic Rb drops and buffer gases were obtained in the cells with a few mm³ internal volumes during the cell sealing process. At an ambient temperature of 90 ℃ the optical absorption resonance of ⁸⁵Rb D1 transition with proper broadening and the corresponding coherent population trapping (CPT) resonance, with a signal contrast of 1.5% and linewidth of about 1.7 kHz, have been detected. The sealing quality and the stability of the cells have also been demonstrated experimentally by using the helium leaking detection and the after-9-month optoelectronics measurement which shows a similar CPT signal as its original status. In addition, the physics package of chip-scale atomic clock (CSAC) based on the cell was realized. The measured frequency stability of the physics package can reach to 2.1×10^-10 at one second when the cell was heated to 100 ℃ which proved that the cell has the quality to be used in portable and battery-operated devices.     

Dynamics of the Mackenzie River plume on the inner Beaufort shelf during an open water period in summer

The oceanographic conditions of the Mackenzie River plume in the Arctic Ocean were examined during a 12-day period in August 2007. Field observations in the river channel and the delta region (2–6 m depth), ship-based observations on the shelf and satellite observations of sea surface temperatures indicate that movements of plume density fronts cause changes in water temperatures of over 10  C over a few days. We used a 1D model to compare the strength of stratification versus surface wind stress, and a 3D numerical model to simulate the plume motions under forcing from the river flows, local wind and water level variations from tides and wind-driven surge. The results indicate that the coastal region is stratified with a ∼2 m thick surface plume even in water depths of 3–4 m, resulting in strong vertical variation of horizontal currents. Moderate easterly winds of 5–10 m/s are sufficient to induce offshore transport of the surface plume and onshore transport of the deeper shelf water, leading to large fluctuations in temperature and salinity in the coastal region. This study examined a period of offshore transport and mean water level set-down, and indicates the rapid response of the plume to wind over the shallow delta.     

湍流度随高度的变化及其对城市宏观地形的依赖

分别构建广州主建成区垂直比例尺为1﹕2 000、1﹕1 000和1﹕500的3个建筑物模型,利用大型边界层风洞,在西北和东南两风向下,基于中性流模拟分析了复杂城市地形下湍流度随高度的变化及其对宏观地形的依赖。结果表明：风廓线指数α与不同高度的湍流度之间的关系密切,利用现有模型,根据4类粗糙度边界层和不同垂直比例尺,可确定相应的湍流度随高度变化模型的主要系数,预测精度高。城市地形下最大湍流度面发育在0~0.2 h之间狭窄的范围内。用湍流度形态指数β来表征湍流度随高度的变化,无论城市屋脊还是平坦地形,随着风程区的延伸,廓线的指数α升高,湍流度形态指数β降低。表明同一高度湍流度值具有由迎风区、丘顶区向背风区增高,沿风程逐渐增大的规律,对地形部位和风程的依赖性强,与来流翻越简单地形时的特征一致。     

鄂尔多斯地块周缘垂直形变场负位错反演与应变积累研究

利用鄂尔多斯地块及其周缘1970～2014年的垂直形变速率场资料,借助负位错反演研究该区域长期应变积累。结果表明,地块东北缘山西断陷带中北段年均能量积累增量、剪应力强度都较高,西南缘六盘山断裂与渭河断裂西段次之;山西断陷带中南段至晋陕交界处年均剪应力强度较高且显示一定程度的能量积累;西秦岭构造区尤其西秦岭北缘断裂西段、晋冀蒙交界区也反映一定程度的能量积累特性。     

凡纳滨对虾性别分化的初步研究

通过组织学连续切片与扫描电镜观察,研究凡纳滨对虾的性别分化。结果显示:凡纳滨对虾外部形态的性别分化最早发生在仔虾后第37天,此时在扫描电镜下可观察到雄性附肢的芽基,仔虾后第45天,光镜下可观察到交接器;仔虾后第24天出现原始生殖细胞(PGCS),位于背大动脉的两侧及围心腔膜的腹面,细胞个体及细胞核明显比周围的细胞大,具有强嗜碱性,细胞核圆形或椭圆形,核内染色质清晰可见,随着生殖腺的发育,在第43天出现生殖管,第55天生殖腺可区分为精巢和卵巢。     

南海及其周缘地区全新世海平面遗迹的构造含义

通过对海南岛海岸带野外考察,在文昌市铜鼓岭石头公园观察到高程为30m的海平面遗迹,在东方市黄流镇观察到高程为25~35m的海平面遗迹,在三亚市鹿回头观察到高程为2.5m的海平面遗迹。综合研究前人在该区关于海平面变化的研究成果,得出如下几点初步认识:(1)南海及其周缘地区中全新世以来海平面呈下降趋势,海平面最高位置出现在7000a左右、高于现代海平面3m左右的位置;(2)南海中全新世以来海平面遗迹所揭示的古海平面高程的差异性是古海平面本身持续下降和该区地壳差异性垂直运动共同作用的结果;(3)南海及其周缘地区中全新世期间中央海盆及其西缘的珠江口、红河、湄公河和昭披耶河4个河流三角洲地区为构造沉降区,而南海周缘的台湾岛、雷州半岛、印支半岛、南马来半岛、爪哇岛和苏拉威西岛6个地区为构造抬升区,抬升幅度最大的地方在台湾岛。     

斜带髭鲷后肠、头肾和脾脏中肥大细胞超微结构观察

应用电镜技术研究了斜带髭鲷的后肠、头肾及脾脏中肥大细胞的超微结构。结果显示:在所研究的器官组织中均发现有肥大细胞,但以后肠和头肾分布较多;这些肥大细胞的表面具有胞质突起或表面折叠。根据其特征性胞浆颗粒所含特殊亚结构的不同,可初步认为斜带髭鲷肥大细胞可能存在Ⅰ型和Ⅱ型两种亚型。Ⅰ型肥大细胞主要分布于后肠粘膜固有层、粘膜下层和头肾的结缔组织中,其特征性胞浆颗粒具有微粒样特殊亚结构;Ⅱ型肥大细胞仅分布于头肾和脾脏的结缔组织中,其特征性胞浆颗粒则具有漩涡样、线团样、蜂巢样、细粒状、无定形和混合型等多种晶格状特殊亚结构。同时,这两种亚型肥大细胞均存在脱颗粒现象。由观察结果可知,斜带髭鲷后肠、头肾及脾脏中存在大量的肥大细胞,并且其特征性胞浆颗粒具有精细的特殊亚结构。     

A Lagrangian mean theory on coastal sea circulation with inter-tidal transports Ⅱ. Numerical Experiments

The results of the new concept of coastal sea circulation are demonstrated by numerical simulations for the first time. The numerical experiments in three types of rectangular model seas illustrate the dependence of circulation on tidal phases due to the convectively nonlinear effect which is estimated by a newly defined drift dispersion in-dex. Then, the present theory is applied in the Bohai Sea of China. At the Bohai Straits and the Huanghe River mouth area the circulation direction even reverses owing to different initial tidal phases which shows that the the-ory copes with nonlinearity well. The calculated M2 tide-induced residual circulation shows that a clockwise gyre exists in the center of an anticlockwise gyre in the central Bohai Sea due to the topographic features. In the Bo-hai Gulf the tide induced circulation shows a 3D structure with outflow at the surface and the inflow at the bottom which can partly explains the spread of the Huanghe River fresh water out of the Bohai Gulf and the inflow of the sediment from the Huanghe River.