热带气旋进入广西影响区强度突然加强的环流特征及物理量诊断分析

本文对1960~2002年进入广西影响区的热带气旋进行统计分析,发现平均每年有4.3个热带气旋进入广西影响区,强度突然加强的平均每年有0.3个,发生在6~10月,其区域分布主要集中在海南岛东北部近海至粤西近海区域和北部湾海域。利用《台风年鉴》和《历史天气图》以及欧洲中心NCEP再分析资料,对这类热带气旋进行环流特征诊断分析,得出了一些结论,有助于更好地做出这类热带气旋的风力和降水预报。     

浮游植物metacaspase的分布、结构及其功能特性

精氨酸/赖氨酸特异性半胱氨酸蛋白酶(Metacaspase)在浮游植物程序性细胞死亡(programmed cell death.PCD)过程中起着关键作用。本文利用生物信息学方法分析metacaspase基因在浮游植物中的分布,并以海洋球石藻(Emilianiahuxleyi)metacaspases(EhMCs)为代表分析其结构和功能,依据Uniprot和String数据库挖掘与metacaspase功能相关并参与浮游植物PCD过程的互作蛋白。结果发现:共计29条、四种类型的metacaspases分布于11种浮游植物中,它们的metacaspase蛋白一级结构存在明显差异,但位于p20的组氨酸和半胱氨酸活性位点均具有高度的保守性;EhMCs三级结构与酵母的metacaspase十分相似,暗示它们具有类似的功能;浮游植物metacaspases与多种蛋白存在直接或间接的互作关系,在细胞PCD过程、响应环境胁迫和能量代谢等方面发挥重要作用。另外.metacaspase的磷酸化修饰可能在浮游植物PCD信号转导过程中起调节作用。     

多层砂土地基扩底桩单桩抗压模型试验及颗粒流模拟研究

利用室内半模试验和颗粒流数值模拟,揭示多层砂土地基扩底桩单桩抗压承载特性及变形特征。结果表明,通过对比分析极限承载力与H_h/D(持力层厚度与扩大头直径之比)的关系可以看出,单桩的抗压极限承载力随H_h/D逐渐增加,当H_h/D超过2.0时,极限承载力基本不再增加,此时的单桩抗压极限承载力稳定在300.01~303.25 N,是H_h/D=0.5时极限承载力(183.83 N)的1.65倍。扩大头下部土体发生局部压缩-剪切破坏,破坏面从扩大头底面边缘向斜下方扩展,在水平方向影响范围达到最大后逐渐向桩内侧收缩;荷载作用越大,地基破坏区域越大,相应的极限抗压承载力也越大;持力层厚度增加,扩大头分担的荷载比例增大,分担的荷载达到稳定需要的桩顶位移也越大,H_h=0.5 D试验扩大头分担的荷载比例稳定时为60%,对应的桩顶位移约为29 mm;桩顶位移达到33 mm后,H_h=1.0~3.0 D试验稳定在63%~65%之间;通过细观颗粒流理论对砂土移动特性的研究发现,持力层厚度从0.5 D增大至2.0 D,破坏面的起始扩展角度从31°增大至42°。数值模拟研究结果与模型试验数据吻合效果良好,证明该方法分析多层砂土地基扩底桩单桩抗压荷载传递机理是可行的。     

Local Oceanic Vertical Deflection Determination with Gravity Data Along a Profile

Deflections of the vertical (DOVs) over oceans cannot be directly measured, which restricts their applications. A local covariance function of anomalous potential is put forward in this paper in conjunction with the least-squares collocation (LSC) method to compute the oceanic DOVs utilization of oceanic gravity data along a profile. The covariance functions of gravity field quantities have been derived directly as functions of x, y and z without the need to introduce coordinate transformations corresponding to along- or cross-profile components. In the proposed methodology, gravity data along a profile were used to calculate the residual gravity anomaly using the remove-compute-restore technique. The residual gravity anomaly was used to calculate the parameters of the proposed covariance function of the local anomalous gravity field, which was used in the LSC to compute the residual DOVs along the profile. The residual DOVs added model DOVs to recover the DOVs along the profile. The results of a simulation experiment prove that the proposed methodology is feasible and effective.     

Mesozooplankton spatial distribution and community structure in the South Adriatic Sea during two winters (2015, 2016)

Whereas the data on mesozooplankton in the epipelagic offshore Mediterranean Sea are extensive, less information is available about plankton in the deeper layers. The present study aims to describe the vertical and horizontal structure and distribution of mesozooplankton species and their associations down to 1,200 m in the water of the Southern Adriatic Sea. Zooplankton were sampled using a Nansen net of 200‐μm mesh size during two cruises in the winters of 2015 and 2016, extending from the coast to the open sea. In total, 203 zooplankton taxa were identified. The community was dominated by copepods, representing between 67% and 91% of the total abundance. The highest total densities were recorded in the upper layers where a high proportion (up to 36%) of appendicularians was also observed in the first sampled year. Five groups of samples were determined based on their community structure. In 2015 communities were distinct between the 0 and 50 m layer and the underlying one (50–100 m), whereas in 2016 epipelagic waters were inhabited by a more uniform mesozooplankton community. The mesopelagic and deep‐water fauna, especially copepods, showed a relatively stable composition in both sampling years Overall, our study confirms the oligotrophic character of the Southern Adriatic, with occasional density outbreaks of appendicularians under favourable conditions.     

斜带髭鲷(Hapalogenys nitens)血细胞发生及外周血细胞化学染色观察

通过Wright-Giemsa染色对斜带髭鲷[Hapalogenys nitens (Richardson)]的头肾、体肾、脾脏、肝脏四组织涂片、印片的观察,发现其头肾、体肾、脾脏是其主要的造血器官。头肾能发育生成各类型血细胞,体肾能生成红细胞和粒细胞,脾脏能生成淋巴细胞和红细胞。肝脏中未发现幼稚型血细胞。血细胞发生大致经过原始、幼稚和成熟三个阶段。细胞化学染色显示:外周血细胞中,除淋巴细胞外,均有糖原;嗜中性粒细胞含有ACP、ALP、NACE、αNAE和POX;嗜酸性粒细胞含有ACP、NACE和POX;单核细胞含有ACP、αNAE和POX;小淋巴细胞有ACP。研究发现斜带髭鲷是一种抗病能力较强的鱼类。血细胞组织化学染色可作为斜带髭鲷疾病诊断的辅助手段。     

广盐性鱼类渗透压适应性与生理可塑性机制研究

拥有强大的渗透压调节能力对广盐性鱼类的生存至关重要。目前,关于鱼类渗透压调节机制已有不少研究,但均存在较大的局限性。本文从广盐性鱼类渗透压信号转导机制、渗透胁迫的细胞调控机制、渗透调控的内分泌调节机制和无机离子通道和转运蛋白介导的渗透调控等方面对广盐性鱼类的渗透压适应性和生理可塑性机制进行分析,以期从分子、细胞、通路和生理等层次初步探索广盐性鱼类盐度胁迫后的可塑性表型变化和不同盐度条件下的应答机制,为广盐性鱼类的渗透压调节机制的深入研究奠定基础。对广盐性鱼类渗透压适应性与生理可塑性机制研究,有助于研究其环境适应机理,促进野生鱼种的人工化养殖以及新品种的育种从而提高经济效益、社会效益和生态效益,对促进水产养殖学进步以及养殖业的发展具有重要意义,同时,为研究广盐性鱼类的渗透压调节机制开辟新的研究方向。     

一个基于MOM的全球海洋数值同化预报系统

随着国家战略利益的拓展,国家对全球海洋环境预报保障的需求日益凸显。近年来,国家海洋环境预报中心研发并建立我国首个涵盖全球大洋的"全球海洋数值预报系统",该预报系统由MOM4全球海洋环流模式及三维变分同化系统组成。该系统的建立,实现了全球范围海洋环流预报业务全覆盖,为我国探索深海大洋环境的迫切需求提供有力保障,明显提升了我国海洋环境预报能力,体现了我国海洋数值预报技术的发展和进步。该系统的历史回报试验和业务化试运行结果表明其对全球海洋环境要素具有较好的预报能力,其预测结果已经在实际业务中得到了应用,在"雪龙号"极地遇险脱困、马航MH370失联飞机搜救等重大事件的预报保障任务中发挥了重要作用,为我国实施海洋强国战略,推进实施"21世纪海上丝绸之路"的战略构想,应对海上突发事件、维护国家海洋权益等各个方面提供有力的科技支撑和保障,并成为我国全球海洋预报业务的重要参考依据。     

栉孔扇贝精巢组织细胞的体外培养及特征分析

本研究建立了一个栉孔扇贝精巢组织细胞的原代培养体系,鉴定了原代细胞的功能基因表达特征。结果显示,原代培养体系中的精巢细胞以圆形细胞为主,直径为4~11μm,其在体外已存活9个月以上。使用栉孔扇贝vasa基因(生殖细胞标记基因)的地高辛RNA探针和原位杂交技术检测,约98%的原代细胞呈阳性。利用免疫组化技术鉴定了栉孔扇贝SCP3、KLF4、PIWI、C-MYC、SOX9和SOX17在这些细胞中的表达特征,发现这些靶蛋白在精巢原代细胞中的表达特征与栉孔扇贝精巢中的细胞定位基本一致。上述结果表明,本实验所获得的原代细胞以生殖细胞为主,它们保留了栉孔扇贝雄性生殖细胞原有的基因表达特征,可以用于贝类精子发生和性别相关的功能基因研究。     

Numerical study of sand scour with a modified Eulerian model based on incipient motion theory

Application of the standard Eulerian model to simulations of sand scour results in unrealistic phenomena. Therefore, the present work develops a modified Eulerian model based on sand incipient motion theory. The modified model is applied for simulating a two-dimensional single vertical jet and a moving planar jet. The simulation results generally demonstrate fairly good agreement with published results of scour profiles and the velocity contours of the water and sand phases. In addition, equations to describe self-similar scour profiles for the moving planar jet cases are given. The results demonstrate that the modified model efficiently and accurately simulates the two-dimensional sand scour produced by jets, particularly for the moving jet cases.