低分子量肝素的制备及其结构与活性差异(英文)

低分子肝素作为一种抗血栓的多糖药物在临床中已应用了二十多年 ,目前已作为外科预防血栓形成药物 ,并在治疗急性静脉栓塞紊乱方面取代了未分级肝素。因肝素的来源和制备的方法不同使低分子肝素的精细结构不同 ,低分子肝素结构的复杂性 ,使得各产品的生物活性 ,例如抗蛋白酶活性不同 ,从而导致其临床使用的标准不同。该文将对低分子肝素的制备方法及其结构和抗蛋白酶活性的差异进行报导     

肝素酶Ⅰ对合成的肝素五糖的酶解作用及对其抗Xa因子活性的影响

研究肝素酶Ⅰ(来源于Flavobacterumheparinase，EC4.2.2.7)对人工合成的且含有与抗凝血酶Ⅲ特定结合位点的肝素五糖(SHP)的酶解作用，并对酶解作用的动力学进行研究。利用强阴离子高效液相色谱(SAX-HPLC)对酶解混合物进行分离，利用质谱(ESIMS)和核磁共振波谱(1H-NMR)技术对得到的二糖和三糖的结构进行确证。研究结果表明，这种被作为肝素反向试剂的肝素酶Ⅰ可水解人工所合成的肝素五糖，从而使之丧失抗Xa因子活性。     

海洋木栖真菌抗菌活性的初步研究

从福建厦门海沧、集美及漳州浮宫等地采集到的红树林等海洋潮间带的各种腐木样品中分离得到176株海洋木栖真菌,对其进行抗菌活性检测.其结果表明共有96株海洋木栖真菌对大肠杆菌、枯草杆菌及白色假丝酵母中的一种或多种拮抗指示菌具有抑制作用,占供测菌株总数的54.5%.在这些活性菌株中,红树源菌株拮抗比例为30.2%,非红树源菌株拮抗比例为69.8%.具有抗菌活性的菌株主要分布于15个属中,包括木霉Trichoderma、青霉Penicillium、拟青霉Paecilomyces和一些不产孢的分类群.某些稀有真菌如盘多毛孢Pestalotia、茎点霉Phoma也具有一定的抗菌活性.     

海洋细菌抑菌活性菌株的筛选

从不同海区的海洋底质，生物样，水样中分离到480株海洋细菌，其中包括150株芽孢细菌和108株粪大肠杆菌，利用纸片法对其抑菌活性进行检测，结果表明：117株海洋细菌具有抑菌活性，占总分离菌株的24.4%；一般细菌，芽孢细菌，粪大肠杆菌的抑菌活性菌株分别占各供测菌株的17.1%,27.3%,35.2%；抑菌活性菌株的比例与样品来源密切相关，且各类海洋细菌的抗菌谱有很大的不同。     

Effects of salinity downshock on dimethylsulfide production

During time-series observations in Sagami Bay, Japan, the concentration of dissolved dimethylsulfoniopropionate (DMSP_d), a precursor of dimethylsulfide (DMS), was negatively correlated with salinity. In the laboratory, low-salinity shock reduced DMS production rates of the natural bacterial community and induced rapid DMSP release from a dinophyte, Heterocapsa triquetra, suggesting that low-salinity shock reduced DMSP_d consumption but enhanced DMSP_d production, which agrees with the negative correlation between DMSP_d and salinity observed in Sagami bay. In addition, low-salinity shock did not affect DMSP lyase activity of H. triquetra. Low-salinity shock would increase the contribution from algae in DMS production, leading to an increase in potential DMS productivity in the environment.     

Simple determination of dimethylsulfide (DMS) and dimethylsulfoniopropionate (DMSP) using solid-phase microextraction and gas chromatography-mass spectrometry

Solid-phase microextraction (SPME) is a simple, sensitive and less destructive method for the determination of dimethylsulfide (DMS) in seawater. Combined with detection by gas chromatography-mass spectrometry (GC-MS), the method had sufficient sensitivity (minimum detectable concentration of DMS was 0.05 nM), and practical levels of reproducibility (relative standard deviation ≤7%) and linearity (r ² > 0.995) over a wide concentration range (0.5 to 910 nM). The protocol developed was applied to a Sagami Bay water sample to determine concentrations of DMS and DMSP, and in situ DMSP-lyase activity.     

浙江主要气象灾害概述

气象灾害每年都有,只是轻重程度不同。就全国和全省范围来说,很少有真正风调雨顺的年份。本文根据1951～1988年38年的气象历史资料及有关政府部门的材料、简报、报道,统计出台风、洪涝、海上大风和强对流等几种主要气象灾害对浙江经济造成损失和伤亡人数。文中对主要气象灾害的特点、一般规律和灾情作了概述,并列出一些典型实例,提出了预防气象灾害的意见。     

近岸海浪模式在中国东海台风浪模拟中的应用--数值模拟及物理过程研究

较为详细地介绍了基于能量平衡方程的第三代近岸海浪数值模式SWAN（Simulation Waves Nearshore）及其包含的物理过程（风生浪、底摩擦、白浪耗散、深度诱导波破碎、非线性波－波相作用等），并利用该模式对影响杭州湾－长江口沿岸海域的一次台风浪过程进行了模拟研究：模式所需风场由藤田台风风场模型嵌入对应台风特征等压线，并对相应时段的NCAR／NCEPT资料、单站资料进行同化后提供；利用自嵌套的方式提供波谱边界条件；模式模拟的结果与实际海浪观测资料相符较好，在此基础上，研究了底摩擦、深度诱导波破碎、三波相互作用等物理过程联合对近岸台风浪的影响，初步认识了它们在近岸台风浪生成、传播过程中的重要作用。     

Locomotor activity rhythm in the Japanese eel Anguilla japonica elvers

Under artificial LD cycles(6,12,18 L),the elvers of Japanese eel,Anguilla japonica,showed a 24 h cycle of locomotor activity rhythm being most active at light transitions:the eels' activity rose to a primary peak after lights-off,followed by a quiescent period during which they buried into the shelters or lying motionlessly on sand for most of the time,and then reached a secondary peak before lights-on.Elvers could resynchronize their activity rhythm with a new photo cycle within 4 d.Moreover,their activity level at dark phase significantly increased as the light period was prolonged:higher activity levels during shorter dark period.However,the elvers did not display clearly the existence of a circadian rhythm under constant light or dark conditions.The timing of daily activity rhythm evidenced in the Japanese eels may occur through the action of the LD cycles with a weak participation of an endogenous circadian system.In all the LD cycles,over 99% of the activity occurred in the dark phase,indicating that the eels were always nocturnally active no matter what time of day it might be.Under 12 L conditions,the eels' activity level and the time outside sand were significantly elevated both at light and dark phases as temperature increased from 10~15 to 20~25 ℃.The activity rhythm pattern(i.e.,two peaks occurring around light transitions) did not apparently change among temperatures.However,in contrast with the primary activity peaks immediately after lights-off at 20 and 25 ℃,the timing of the primary peaks at 10 and 15 ℃ showed a latency of a few hours following lights-off,indicating the inhibiting effect of low temperature on the eels' activity.     

泰利台风对台湾东北黑潮入侵东海陆架影响的研究

本文利用卫星高度计数据和分析数据,并结合同时期现场深水潜标的流速观测数据,研究了超强台风泰利过境前后台湾东北附近海域流场、位势密度场、位势涡度场以及黑潮入侵东海陆架强度的变化。分析结果表明,泰利台风通过改变台湾东北陆坡附近海域的流场、位势密度场,显著地削弱(增强)了西段(东段)陆坡附近的位势涡度梯度,从而使得西段(东段)陆坡黑潮入侵东海陆架的强度显著增强(减弱)。此外,本文还区分了台湾东北西部陆坡附近表层的跨陆坡“上凸型”位势涡度分布与次表层的跨陆坡“下凹型”位势涡度分布,并认为次表层的跨陆坡“下凹型”位势涡度分布也应作为台湾东北西部陆坡附近“位势涡度障碍”的重要组成部分。本文的研究结果揭示了大气中的台风过程对台湾东北黑潮入侵东海陆架产生显著影响的关键过程及机制,相关结论可为台湾东北黑潮入侵东海陆架变化规律的研究提供有价值的参考。