Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses

The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder Sperm. Sinistral and dextral are two types of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species. Of the 22 loci examined from 12 allozymes,12 confirmed hybridization of the paternal and matemal loci in hybrids and no difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above 38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation could inherit a little more genetic materials from paternal fish than that from maternal fish.     

Immunotoxicity effect of benzo[α]pyrene on scallop Chlamys farreri

The toxic effects of benzo[α]pyrene (B[α]P) at different concentrations (0.1, 0.5, 1, 2.5 and 7.5 μgL⁻¹) on scallop (Chlamy farreri) immune system were studied. The results showed that B[α]P had significant toxic effects on the haemocyte counts, neutral red uptake, phagocytosis, bacteriolytic and antibacterial activity (P<0.05), while the seawater control and acetone control had no significant differences. The haemocyte counts, neutral red uptake, phagocytosis and bacteriolytic activity in all B[α]P treatment groups as well as antibacterial activity in groups of 0.5, 1, 2.5 and 7.5 μgL⁻¹ B[α]P decreased significantly (P<0.05). Some of these indices tended to be stable on the sixth day and others on the ninth day, and the indices showed clear time- and concentration-response to B[α]P. Bacteriolytic activity in 0.1μgL⁻¹ B[α]P treatment group and antibacterial activity in 0.1 μgL⁻¹ and 0.5 μgL- B[α]P treatment groups increased at the beginning of exposure and reached their peaks on day 1 and day 6, respectively. Following that, both activities decreased gradually and became stable after day 9. When all the indices reached stability, they were significantly lower than those in control group (P<0.05), except for antibacterial activity in 0.1 μgL⁻¹ B[α]P treatment group (P>0.05). Thus, B[α] has evident toxic effects on scallop immune system, which supports the view that a relationship exists between pollution and immunomodulation in aquatic organisms.     

Adaptation management of mountain tourism service: the case of the source regions of the Yangtze and Yellow River

Mountain areas are often rich in ecological diversity and recreational opportunities. Mountain tourism is thought to be an effective and important means for maintaining and expanding rural economies and, thus, improving the living conditions of rural societies. As mountain tourism service research is a professional field with several disciplines involved, a multi-disciplinary management pIatform is needed and it facilitates participation in sustainable mountain development by diverse stakeholders. With the source regions of the Yangtze and the Yellow River as a case study, this paper presents a conceptual framework for an adaptation management of mountain tourism services according to technical, policy, social and economic dimensions. The framework is based on a vulnerability assessment of mountain ecosystems, and can serve as a reference for the development of tourism service in other mountain areas.     

人工育珠对三角帆蚌(Hyriopsis cumingii)遗传基因影响的初步研究

采用酚-氯仿和试剂盒两种提取法对三角帆蚌怀珠群与非怀珠群各6个个体进行基因组DNA的提取,然后在优化RAPD(随机扩增多态性DNA)检测条件基础上,从80个随机引物中筛选出12个扩增较好且多态性强的引物进行RAPD扩增,产物通过水平板琼脂糖凝胶电泳和垂直板聚丙烯酰胺凝胶电泳两种方法进行检验并对DNA的多态性进行分析。结果显示在怀珠群和非怀珠群检测到的位点数、多态位点比例、平均Shannon多态性信息指数、平均Nei's基因多样性指数、群体内个体间平均遗传相似率和平均遗传距离分别为100、33%、0.1927、0.1324、0.904、0.096,95、47.37%、0.2711、0.1879、0.861、0.139,群体间的平均遗传距离为0.821,非怀珠群的变异性大于怀珠群;本研究还获得了两群体各自的特异扩增谱带及两群体间表达差异较大谱带,这些位点很可能是由人工育珠所引起。根据MEGA4.0软件的UPGMA和NJ程序构建的分子系统树可直观地将两群体分开。     

台风作用下淤泥质海岸动力地貌响应

基于200509号麦莎台风(近似百年一遇)作用前后杭州湾北岸龙泉-南竹港实测的海岸地形和水文资料,利用分形技术、冲刷深度和闭合水深等相关概念分析淤泥质海岸的剖面变化、岸线进退以及地形冲淤,进而探讨海岸响应台风作用的动力地貌行为.结果表明:1)台风作用前后的海岸动力地貌过程表现为水下类似沙坝的堆积体削平、凹处填洼,水下平台发育锯齿或较小的坝--槽,海岸剖面的分维数降低,剖面坡度处于平缓;2)麦莎台风对海床的整体冲淤幅度不大,这可能是由于研究区大潮讯的增水致使波浪对海床的塑造能力减弱以及码头的遮蔽作用造成;3)台风作用对海岸的冲刷深度约为0.8 m,闭合水深为9.5 m,近岸-1 m邻近区成为海岸响应台风作用最敏感的地带;泥沙主要在0 m到闭合水深区间的海床发生纵、横向运移.     

利用海底地震仪数据分析台风对海底环境噪音的影响

在海底布设的海底地震仪（OBS）能比较清晰地记录到海底的环境噪音,而台风可以直接或间接的产生在海底传播的弹性波,从而影响海底的环境噪音,并在较大程度上影响OBS的数据记录。本文通过分析台风对工作区的整个影响过程中OBS记录数据的振幅变化,再选择合适的滤波方式,首次发现台风产生的风浪及涌浪在短周期海底地震仪的记录数据上有良好的表现特征,指出了台风对海底环境噪音的另一种可能的影响方式,并由此得出：1）台风产生的风浪和涌浪对海底环境噪音的影响模式不同;2）风浪和涌浪所加强的海底环境噪音的范围和程度不同;3）短周期OBS可以比较清晰的记录涌浪信息,其周期主要是6—8 s,且能量稳定（简称“8秒现象”）。这三点结论为后期的海洋地震研究和海洋学其他研究提供经验与借鉴。     

Exploring uncertainty in remotely sensed data with parallel coordinate plots

The existence of uncertainty in classified remotely sensed data necessitates the application of enhanced techniques for identifying and visualizing the various degrees of uncertainty. This paper, therefore, applies the multidimensional graphical data analysis technique of parallel coordinate plots (PCP) to visualize the uncertainty in Landsat Thematic Mapper (TM) data classified by the Maximum Likelihood Classifier (MLC) and Fuzzy C-Means (FCM). The Landsat TM data are from the Yellow River Delta, Shandong Province, China. Image classification with MLC and FCM provides the probability vector and fuzzy membership vector of each pixel. Based on these vectors, the Shannon's entropy (S.E.) of each pixel is calculated. PCPs are then produced for each classification output. The PCP axes denote the posterior probability vector and fuzzy membership vector and two additional axes represent S.E. and the associated degree of uncertainty. The PCPs highlight the distribution of probability values of different land cover types for each pixel, and also reflect the status of pixels with different degrees of uncertainty. Brushing functionality is then added to PCP visualization in order to highlight selected pixels of interest. This not only reduces the visualization uncertainty, but also provides invaluable information on the positional and spectral characteristics of targeted pixels.     

GISVlog模型的设计与实现

本文以地理信息系统(GIS为基础,分析了地理信息系统沃客(GISVlog,)形成的两个背景——网络新技术提高GIS生命力和沃客(Vlog,Vedio log的缩写)为网络增值;分别论述了GISVlog形成对GIS发展和Vlog发展的意义;在普通GIS的系统功能结构和Vlog的系统功能结构的基础上构建GISVlog的框架结构并加以实现;论文最后展望了GISVlog的发展前景,提出GISVlog是网络搜索技术新的研究方向。     

2006年台风“碧利斯”与“格美”的降水差异性比较

对2006年7月登陆,西移影响粤东的路径相似、降水过程强度和范围不同的2次台风过程,从环流形势,台风环流本身、结构以及降水性质等方面进行了对比分析.结果表明,"碧利斯"的降水是一典型的和西南季风相结合的降水,而"格美"的降水仅仅是台风本身环流的降水.同时发现两者在登陆2 d内500 hPa平均高度场、水汽通量垂直剖面图,水汽输送时间序列图上均有显著差异.指出水汽输送的强弱,决定了登陆台风的维持时间和降雨强度.