Diagnosis of iridovirus in large yellow croaker by PCR

A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10^-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection.     

Validation and Improvement of Satellite-Derived Surface Solar Radiation over the Northwestern Pacific Ocean

The purpose of this study is to validate and improve satellite-derived downward surface shortwave radiation (DSSR) over the northwestern Pacific Ocean using abundant in situ data. The DSSR derivation model used here assumes that the reduction of solar radiation by clouds is proportional to the product of satellite-measured albedo and a cloud attenuation coefficient. DSSR is calculated from Geostationary Meteorological Satellite-5/Visible Infrared Spin-Scan Radiometer data in 0.05° × 0.05° grids. The authors first compare the satellite DSSR derived with a cloud attenuation coefficient table determined in past research with in situ values. Although the hourly satellite DSSR agrees well with land in situ values in Japan, it has a bias of +13∼+34 W/m² over the ocean and the bias is especially large in the low latitudes. The authors then improve the coefficient table using the ocean in situ data. Usage of the new table successfully reduces the bias of the satellite DSSR over the ocean. The cloud attenuation coefficient for low-albedo cases over the ocean needs to be larger in the low latitudes than past research has indicated. Daily and hourly DSSR can be evaluated from the satellite data with RMS errors of 11–14% and 30–33%, respectively, over a wide region of the ocean by this model. It is also shown that the cloud attenuation coefficient over land needs to be smaller than over the ocean because the effect of the radiation reflected by the land surface cannot be ignored.     

A finite element model of interaction between viscous free surface waves and submerged cylinders

This paper describes the simulation of the flow of a viscous incompressible Newtonian liquid with a free surface. The Navier–Stokes equations are formulated using a streamline upwind Petrov–Galerkin scheme, and solved on a Q-tree-based finite element mesh that adapts to the moving free surface of the liquid. Special attention is given to fitting the mesh correctly to the free surface and solid wall boundaries. Fully non-linear free surface boundary conditions are implemented. Test cases include sloshing free surface motions in a rectangular tank and progressive waves over submerged cylinders.     

无公害刺参稳产健康养殖新技术

本文针对目前刺参养殖中存在的问题,简单明了叙述刺参无公害健康养殖一系列新技术和新方法,对推动海参养殖业持续、稳定、健康发展,起到积极作用和效果。     

Summer Arctic sea fog

I~IOXSea fog is a kind of dangerous weather. Chinese sea fog experts, Wang Binhua (1983),Hu Ruijin and Zhou Faxiu (1998) and Hu Jifu et al. (1996) studied sea fog rather Systematically. FOreign Experts also Paid great attention to sea fog. Ernlnons and Montgomery(1974), chipper (1994) and Rayrnond et al. (1989) have studied sea fog thorOUghly.HOwever, studies on Arctic sea ice have rarely been carried Out becauSe of the sever environment and less htnnan activity in the region. There …     

肝素酶Ⅰ对合成的肝素五糖的酶解作用及对其抗Xa因子活性的影响

研究肝素酶Ⅰ(来源于Flavobacterumheparinase，EC4.2.2.7)对人工合成的且含有与抗凝血酶Ⅲ特定结合位点的肝素五糖(SHP)的酶解作用，并对酶解作用的动力学进行研究。利用强阴离子高效液相色谱(SAX-HPLC)对酶解混合物进行分离，利用质谱(ESIMS)和核磁共振波谱(1H-NMR)技术对得到的二糖和三糖的结构进行确证。研究结果表明，这种被作为肝素反向试剂的肝素酶Ⅰ可水解人工所合成的肝素五糖，从而使之丧失抗Xa因子活性。     

基于EMD与神经网络的机械故障诊断技术

经验模式分解 (EMD)是分析非线性、非平稳信号的有力工具 ,它将信号分解为突出了原信号的不同时间尺度的局部特征信息的内在模函数 (IMF)分量。本文通过将各 IMF分量输入到 BP网络中进行训练学习和故障诊断 ,比直接输入原信号可以提高 BP网络对故障诊断的准确率 ,而且减少了训练时间。     

鲈鱼胆汁中芘及代谢产物含量与水体中芘含量的关系

运用GC—MS的方法和荧光分光光度法，研究鲈鱼分别暴露于0．1、1．0和10．0μg／dm^3质量浓度芘溶液中7d，水体中芘及其鲈鱼胆汁中芘和1-羟基芘含量的变化,实验结果显示：(1)鲈鱼对水体中的芘具有非常显著的去除作用。(2)随着芘暴露浓度的增大，鲈鱼对芘的代谢去除作用增强。(3)随着暴露时间的延长和芘暴露浓度的增大，胆汁的1-羟基芘浓度递增。(4)鲈鱼胆汁具有较高的芘浓度，对海水中的芘具有较强的富集作用。(5)鲈鱼胆汁的1-羟基芘及芘的浓度与水体芘浓度均具有很好的相关性，对于指示水体的芘污染程度具有一致性；可作为指示水体芘污染程度的生物标志物。     

围隔生态系中叶绿素a浓度、化学耗氧量及透明度之间统计关系的研究

通过对水库中12个围隔两次取样的叶绿素a浓度(单位:μg/L)、化学耗氧量(单位:mg·O_2/L)和塞克透明度(单位:m)的实验数据分析,求得如下回归方程: (1)[Chla]=17.60·[SD]~(-1·86) r=-0.87 (2)[Chla]=-28.85+7.32·[COD] r=0.89 (3)[COD]=6.88·[SD]~(-0·68) r=0.91本文还对这些结果进行了讨论。     

两参量的海面阻力系数模式的探讨

从风浪的能量平衡方程出发，引进若干风要素与波要素以及波要素之间的定性关系，经演算可导出海面阻力系数（Ｃｐ）或是风速（Ｕ）和波龄（β）或是Ｕ和波高（Ｈ）的函数，然后沿用最小二乘法，终将得出４组１２个回归方程。当β（或β）或Ｈ为某一给定值，惟有Ｕ为唯一参量时，所提各式均可简化为非线性方程：ＣＤ＝ａ＋ｂ，Ｕ＋ｃ．Ｕ＾２；式中ａ，ｂ和ｃ为三个经验系数，就所检验的例子而言，本文的结果与实际的符合前人的为好。