大黄鱼(Pseudosciaena crocea)转铁蛋白(Tf)基因克隆及序列分析

提要应用RNeasy animal mini试剂盒抽提大黄鱼total RNA,逆转录合成模板cDNA;同时综合分析从GenBank数据库查询到已报道的转铁蛋白(Tf)基因序列,先设计并合成扩增引物扩增出大黄鱼转铁蛋白部分基因序列,再应用RACE技术,克隆出大黄鱼转铁蛋白全长cDNA基因,全长2461个核苷酸,编码661个氨基酸。应用WCG软件包,采用DNADIST和NEIGHBOR分析方法,构建了转铁蛋白系统进化树。树图显示与传统的分类地位大致相符,可以看出转铁蛋白在海水鱼和淡水鱼的进化上的差异,同时也显示大黄鱼Tf与真鲷的同源性最高。     

Satellite detection of red tide in Ariake Sound, 1998–2001

High resolution SeaWiFS data was used to detect red tide events that occurred in the Ariake Sound, Japan, a small embayment known as one of the most productive areas in Japan. SeaWiFS chlorophyll data clearly showed that a large red tide event, which damaged seaweed (Nori) cultures, started early in December 2000 in Isahaya Bay, expanded to the whole sound and persisted to the end of February 2001. The monthly average of SeaWiFS data from May 1998 to December 2001 indicated that the chlorophyll peaks appeared twice a year, in early summer and in fall, after the peaks of rain and river discharge. The SeaWiFS data showed that the red tide event during 2000–2001 winter was part of the fall bloom; however, it started later and continued significantly longer than other years. Satellite ocean color data is useful to detect the red tide; however the algorithms require improvement to accurately estimate chlorophyll in highly turbid water and in red tide areas.     

A climatology of mesoscale model simulated low-level wind jets over Cook Inlet and Shelikof Strait, Alaska

Weather in the North Gulf of Alaska is characterized by a high frequency of deep synoptic-scale low-pressure systems, especially during the cold season. The strong pressure gradients of these storms interact with the extremely rugged terrain of the coastal mountains to produce a variety of channeled flows. These surface wind regimes are not well documented in the scientific community, due to the paucity of observations. Modeling of these phenomena in regions of complex terrain is of great interest to those working with hydrodynamic, wave, and pollutant transport models in coastal and shelf areas. Such models, when coupled with ocean and coastal-ecology counterparts, give a broad view of the role surface winds play in shaping local coastal marine ecosystem in this region. This paper presents a climatology of simulated low-level wind jets over the domain of Cook Inlet and Shelikof Strait along Alaska's south-central coast. Daily simulations using the RAMS model were conducted in a 36-h forecast mode for the cold-season period 10/1/03 to 3/31/04. Systematic analysis of the resulting simulated low-level wind field makes it possible to characterize these jets and gap flows in spatial and temporal detail. The comparison between the RAMS winds and the Synthetic Aperture Radar (SAR)-derived winds when available verifies the existence of these wind jets and the capability of the model to simulate these cases. Clearly, the results of a study in this region depend on the fidelity of the model at these scales (O[5 km]). The SAR comparisons attempt to help establish this. From the 6 months of simulations over Cook Inlet and Shelikof Strait, the low-level wind jets are classified into 10 different regimes by location and orientation. These regimes are categorized into four more general groups: cross-channel westerly, easterly, and up and down Inlet flows. The nature of a particular regime is largely a function of pressure gradient orientation and local topography. Jets in the same group have a similar occurrence distribution with time. Some form of jet occurred in the study region almost daily each month of the period, with December 2003 having the highest frequency of wind jets.     

黄、东海环流的数值研究Ⅲ——正压环流的数值模拟

依据黄、东海环流的的动力学模型 ,运用“流速分解法”对黄、东海正压环流进行了数值模拟。计算结果表明冬季黄海正压环流主要受风应力影响 ,基本形态为黄海暖流由济州岛西南进入南黄海中部 ,其东西两侧分别为两支向南流动的沿岸流 ;夏季主要受到潮致体力的影响 ,为一逆时针涡旋。东海环流主要是边界力作用驱动的结果 ,东海黑潮、台湾暖流和对马暖流较稳定。冬季风应力对东海环流表层流场有消弱作用 ,在夏季则有一定增强作用。     

梭鱼标准代谢、内源氮排泄与体重和温度的关系

在 13.5 ,18,2 1.5 ,2 4和 2 7℃ 5个温度条件下测定了梭鱼 (体重范围 1.88～ 14.0 2 g)的标准代谢率和内源氮排泄率。梭鱼标准代谢率随体重的增加而增加 ,二者的关系为幂函数关系 ;随温度的升高而增加 ,二者的关系为指数关系 ;标准代谢率与体重和温度的关系可用如下方程表示 :RS=0 .12 4 6 W0 .9954 e0 .0 84 1T(r2 =0 .92 2 0 )。梭鱼的氨氮、尿素、总氮及能量的排泄率随体重和温度的增加而增加 ,与体重为幂函数关系 ,与温度的关系为多项式形式。梭鱼氨氮和尿素日排泄率的变幅分别为 0 .15～ 0 .88mg N/d和 0 .0 3～ 0 .2 9mg N/d。不同温度下 ,尿素排泄量占总氮比例在 9.9%～2 2 .4 9%之间 ,随温度升高该值有逐渐增大的趋势。     

东海陆架北部表层细粒级沉积物的级配及意义

本文依据东海陆架北部 2 3个表层沉积物样品的粒度分析资料 ,就东海陆架北部表层细粒级沉积物的级配及意义进行了研究。结果表明 ,研究区表层沉积物中细粒级部分以 <0 .0 16 mm为优势粒级 ,可占到总细粒级沉积物的 75 %以上。济州岛西南泥质区以细于 0 .0 0 8mm粒级的沉积物占据优势 ,长江口泥质区则以 <0 .0 16 mm粒级的沉积物为主 ,两泥质区细粒级沉积物的级配很不相同     

使用简易测试平台检测马哈克扭力仪的性能

本文描述简易测试平台的原理与制作，对马哈克MAIHAK MDS－820型扭力仪检测模拟负载的准确性进行探讨。     

湄洲湾表层沉积物中重金属的地球化学特征

根据1984年湄洲湾表层沉积物中重金属的分析资料,讨论了它们的地球化学特征和主要来源。沉积物中重金属的含量(Fe、Mn、Cu、Ni 的质量分数均值分别为3.21%、730×10~(-6)、19.4×10~(-6)、44×10~(-6)分布比较均匀,Pb、Zn 和Co 含量(质量分数均值分别为47.0×10~(-6)、25.8×10~(-6)和243×10~(-6))偏高,微量金属含量不受Fe-Mn 氧化物和有机物控制,本地区的金属来源受陆源输入的影响明显。     

Distribution of As, Cr and V species in the Po-Adriatic mixing area, (Italy)

The distribution of dissolved and participate concentrations of As, Cr and V was investigated in the mixing area of the Po river in five surveys over the period March 1992-March 1995. The data obtained indicate noticeable contamination of coastal waters of the northern Adriatic with Cr but not As or V. Total dissolved concentrations for As and V were comparable with oceanic levels but were higher by a factor of 4 for Cr. These results suggest either low anthropogenic inputs of As and V, from terrigenous and atmospheric sources, or efficient removal mechanisms. The investigated metals had low reactivity during freshwater-seawater mixing and the distribution of concentrations in the dissolved and solid phases suggests that the dilution process is the main factor controlling the transport of these metals to the sea in low-to-medium solid transport conditions. Further investigations are required to elucidate the behavior of these metals during high solid transport conditions. Significant changes in the speciation of As occurred during the transition between the two end-member matrices. In the coastal waters of the northern Adriatic, the arsenite fraction in winter, and the arsenite and dimethylarsinate fractions in summer increased with respect to riverine waters. Changes in the speciation of chromium were minor, but also showed a small increase in reduced species in marine waters.     

鳗弧菌(Vibrio anguillarum)M3菌株生长条件及其对蛋白酶产量的影响

采用体外测定细菌浓度、胞外产物(ECP)蛋白含量和蛋白酶活力的方法，进行了鳗弧菌M3菌株在2216E培养基中的培养条件研究。结果表明，该菌株用固体培养基培养至24h左右，可得到较高的菌体浓度、ECP蛋白含量和蛋白酶活力。采用响应面分析方法设计实验，用SAS统计软件分析数据，得到NaCl浓度、pH值和温度对菌体生长及蛋白酶产量影响的回归模型。在2216E培养基的基础上，添加不同氮源、碳源物质以及不同浓度蛋白胨进行生长研究。结果表明，胰大豆蛋白胨能促进菌体生长及ECP蛋白分泌；NH4Cl与酪蛋白水解物可抑制蛋白酶的产生；牙鲆肌肉匀浆对菌体、ECP蛋白产量和蛋白酶产生有不同程度的促进作用；培养基中蛋白胨浓度为4％时菌体量与ECP蛋白含量达最高值，在蛋白胨浓度为2％时蛋白酶分泌量已稳定；1％的葡萄糖、蔗糖、甘油均能显著地提高菌体及ECP蛋白产量，却抑制了蛋白酶的产生。