基于核酸适配体功能化石墨纳米颗粒荧光探针的17β-雌二醇快速检测方法

为构建一种能够简单、快速、特异性检测复杂环境水体中雌激素污染的方法,利用石墨纳米颗粒作为荧光淬灭剂、核酸适配体作为识别元素、1-芘丁酸N-羟基琥珀酰亚胺酯作为异型双功能交联剂,构建了一种新型纳米荧光探针;并探究了构建荧光探针时核酸适配体初始投加量和荧光探针投加量对雌二醇检测的影响及最佳实验条件下检测雌二醇的效果和特异性。实验结果表明：核酸适配体能成功修饰在石墨纳米颗粒表面形成的稳定荧光探针;构建荧光探针时核酸适配体的最佳初始投加量为1.0 μmol/L;检测雌二醇时,荧光探针的最佳投加量为4 μg/mL;最佳实验条件下,相对荧光强度与雌二醇的质量浓度在50~800 ng/mL范围内成正比,最低检测限为34.5 ng/mL,且该荧光探针能实现对雌二醇的简单、快速、特异性检测。     

Effects of the peat acid digestion protocol on geochemically and morphologically diverse tephra deposits

Tephra shards for electron probe microanalysis are most efficiently extracted from peat using acid digestion, which removes organic material that hinders density separation methods. However, strong acids are known to alter glass chemical compositions, and several studies have examined how acid digestion affects rhyolitic volcanic glass. The focus on rhyolitic tephra in these studies leaves considerable uncertainty, as the dissolution rates of natural glasses (including tephra) are determined by the chemical composition and surface area/volume ratio, both of which vary in tephra deposits. Here, we use duplicate samples of basaltic, trachydacitic and rhyolitic tephra to examine physical and geochemical alteration following acid digestion. Scanning electron microscope imagery reveals no discernible degradation of glass surfaces, and electron probe microanalysis results from duplicate samples are statistically indistinguishable. These findings suggest the acid digestion protocol for organic peats does not significantly alter glass geochemistry regardless of shard morphologies or geochemical compositions.     

变异函数模型参数的非线性加权总体最小二乘法

针对球状模型和指数模型两种变异函数模型线性化后的系数矩阵具有非线性形式,系数矩阵元素含有随机误差等问题,该文使用非线性加权总体最小二乘法估计变异函数模型参数。以高程异常数据为例,利用变异函数值的点对数定权法和系数矩阵中距离值的方差-协方差传播律定权法迭代解算参数,并与最小二乘法,加权最小二乘法和非线性总体最小二乘法进行对比分析。实验结果表明:非线性加权总体最小二乘法能够得到更高精度的变异函数模型参数。     

应用Slepian局部谱方法解算中国大陆重力场球谐模型

根据经典的球谐函数方法,为满足正交化要求,观测数据需要覆盖整个球面,而对于地表局部测量数据,则无法应用球谐方法解算重力场模型。针对此问题,采用Slepian局部谱分析方法解算中国大陆范围内的实测重力场变化数据,并以GOCE卫星球谐函数解作为已知模型,评估由于实际陆地重力测点的非均匀分布对球谐函数解的误差影响。通过计算多个阶次中国大陆局部范围的Slepian基函数分布;采用GOCE卫星获得重力场模型的前72阶球谐系数作为已知结果,评价实际测点非均匀分布的解算有效性,并针对中国大陆地区采用Slepian基函数进行解算,通过模型对比选择最优截段项数;针对2005—2008年中国大陆地区流动重力测量获得的重力场变化信号进行解算,获得了72阶重力场变化模型。     

扰动重力梯度的球冠谐分析建模

从球冠谐理论出发,详细推导了球冠坐标系下扰动重力梯度的无奇异性计算公式。基于Tikhonov正则化方法,利用GOCE卫星实际观测数据解算局部重力场球冠谐模型。数值计算表明,基于扰动重力梯度的球冠谐分析建模方法能够有效地恢复局部重力场中的短波信号,与GO_CONS_GCF_2_DIR_R5模型的差异在±0.3×10~(-5) m/s~2水平。     

A discrete global grid system for earth system modeling

To support Earth system modeling, we propose a discrete global grid system that expresses multi-resolution spatial data. Specifically, a unified coding model that expresses a grid of nodes, edges, and cells is constructed for a triangular discrete global grid system. To fulfill the requirements of practical applications, we design a code-based topological query method for this grid system and an algorithm to transform between grid codes and geographic coordinates. We evaluate the Global Finite Volume Community Ocean Model (Global-FVCOM) on the triangular discrete global grid system in the proposed uniform coding model. The ocean tidal waves simulated by the Global-FVCOM running on the coded grid are then compared with results obtained using a traditional irregular spherical grid system, and the results display comparable accuracy. The uniform coding model proposed in this paper provides a triangular discrete global grid system that can represent multi-resolution spatial data and can be used in Earth system models. This unified coding model can also be applied to the geographic coordinate system made up of latitudes and longitudes, as well as diamond and hexagonal grids.     

球谐分析及合成并行计算

本文介绍了球谐合成和分析问题的数值计算原理,研究分析了其内在的并行性,提出了区域划分和聚合通信的并行计算策略。基于傅立叶变换技术,应用MPI(消息传递接口)并行编程模式,实现了球谐分析及合成计算的并行化。实验结果表明,较之串行实现,并行实现大大缩短了计算时间,获得了近似线形加速比,取得很高的并行效率。     

福建东山岛矿产资源开发利用与生态环境保护初论

就东山岛在水、砂、石、土的开发利用中存在的问题,阐述了开发利用矿产与保护生态环境之间的辩证关系,提出东山县应做好矿产资源开发利用与“建设港口旅游城市”的规划,走资源节约型的发展海洋经济建设的新路子,促进东山县经济的可持续发展。     

Influence of environments of coal deposition on phosphorous accumulation in a high latitude, northern Alaska, coal seam

Although phosphorus is a very important biogenic element, its concentration in coal is generally low. Phosphorus (P) concentrations typically range from 0.001% to 0.229% in raw coals of the contiguous 48 states [Glick, D.C., Davis, A., 1984. Variability in the inorganic content of United States coals—a multivariate statistical study of final report, Part 10 (DOE-30013-Flo) to the US Dept. of Energy under contract no. DE-AC22-80PC 30013, 404 pp.]. Some Alaskan coal seams contain horizons that are unusually high in phosphorus. The present paper focuses on a bituminous coal from northern Alaska, where 0.15 m subsections of this coal seam have shown high phosphorous in certain subsections. In order to investigate the lateral extent of such high phosphorous bands in coal, the authors obtained three drill cores up to 3 km apart from the coal seam. A detailed investigation of the cores was undertaken to determine the nature and mode of occurrence of phosphorous minerals. Maceral composition allowed interpretation of environments of coal deposition. Acid-extractable phosphorous analysis of the subsections identified the high phosphorous horizons. Electron microprobe analyses identified the phosphorous mineral as crandallite. Correlation of high phosphorous intervals with corresponding environments of deposition suggests that phosphorous precipitation is promoted by an oxidizing environment with a lowered water table during the peat stage. A study of thin sections from high phosphorous samples showed that crandallite is associated with structured vitrinite and as cell fillings in fusinite. The study confirms the potential for using high phosphorous horizons for the correlation of coal seams, as noted in previous work by the authors (Rao and Walsh, 1997). A high phosphorous horizon is found at 0.45 m above the bottom of the seam in all of the drill holes, indicating a uniformity of the coal forming environment and the availability of crandallite constituent elements over the entire 3 km. The uniformity of high phosphorous concentrations through the top 2 m of the three drill holes also shows a distinct correlation.