Rapid detection of virulent protease secreted by Vibrio anguillarum by dot enzyme-linked immunosorbent assay

Dot enzyme-linked immunosorbent assay （dot-ELISA）, indirect ELISA and Westem blot were performed to detect the virulent protease secreted by Vibrio anguillarum which was isolated from the diseased left-eyed flounder, Paralichthys olivaceous. Sensitivity results showed that dot-ELISA is a more sensitive, rapid and simple technique for the protease detection. The minimal detectable amount of protease is about 7 pg in the dot-ELISA test, while 7.8 ng in the indirect ELISA and 6.25 ng in the Westem blot respectively. Protease could be detected 2 h after incubation of V. anguillarum in the 2216E liquid medium but enzyme activity was very low at that period. From 6 to 12 h, the amount and enzyme activity of protease increased markedly and reached maximum at stationary phase. Analysis of serum samples periodically collected from the infected flounders showed that after 2 h of infection by V. anguillarum, the pathogenic bacteria could be detected in the blood of the infected flounders but no protease was found. It was 5-6 h after infection that the protease was detected in blood and then the amount increased as infection advanced. Quantitative detection of protease either incubation in the medium or from the blood of infected flounders could be accomplished in virtue of positive controls of quantificational protease standards （＂marker＂） so that the alterations ofprotease secretion both in vitro and in vivo could be understood generally. In addition, the indirect ELISA and dot-ELISA were also performed to detect V. anguillarum cells. Results indicated that the sensitivity of indirect ELISA to bacteria cells is higher than that of the dot-ELISA, and that the minimal detectable amount is approximately 10^4 cell/mL in the indirect ELISA, while 10^5 cell/mL in the dot-ELISA.     

沉降监测多项式回归分析与神经网络预测

使用多项式和切比雪夫(Tchebyshev)多项式分别对沉降监测数据进行回归分析以预测未来沉降值,其中切比雪夫多项式的外推效果较好;应用前向BP神经网络对两种不同的单因子输入模式进行非线性函数逼近,并进行了不同采样步长的比较,实例表明将时间点作为网络的输入对沉降进行预测效果较好。     

关于“包装产品的计量标准型一次抽检方案”的确定

本文给出了“包装产品的计量标准型一次抽检方案”,并通过一个实例说明本方案比一般《计量标准型一次抽检方案》更为优越。     

Theory and Practice of Soft Clay Engineering

-The shear strength and deformation properties of soft clay are discussed first. Then some methods for predicting the performance of soft clay foundation are proposed. Finally, case histories are presented to illustrate some discussed aspects of soft clay.     

台湾海峡及其西边地区正常地震动态及危险性特征

本文从地震的时，空分布特征，区域应力场动态，能量释放方式，ｂ值及震群特征６个方面研究了台湾海峡及其西边地区地震活动的正常动态及异常特征。结果表明，具有前兆意义的变化模式表现为区域地震活动在时间，空间及功能方面的有序性变化，即：（ａ）地震空间分布由分散转为集中，形成条带或空区；（ｂ）断裂活动由多组转为单一，应力场趋向一致；（display status     

Implementation of High Resolution Tidal Current Fields in Electronic Navigational Chart Systems

A system for displaying tidal currents in an electronic chart display and information system (ECDIS) has been developed and implemented in compliance with the standards of the International Hydrographic Organization (IHO). The tidal current fields can be displayed in real time on the electronic navigational chart and several options and functions for updating and zooming have been designed. The current fields are calculated from a data base with the harmonic constants for the four major tidal constituents. The harmonic constants are obtained from a high resolution numerical model with horizontal grid resolution of 100 m. The model is validated by comparing with sea level and current measurements. The depth matrix for the central part of the model domain was calculated from data from multibeam bathymetric surveys. An application example of the implementation is given for Trondheimsleia, a part of the main sailing route along the western coast of Norway.     

近岸海浪模式在中国东海台风浪模拟中的应用--数值模拟及物理过程研究

较为详细地介绍了基于能量平衡方程的第三代近岸海浪数值模式SWAN（Simulation Waves Nearshore）及其包含的物理过程（风生浪、底摩擦、白浪耗散、深度诱导波破碎、非线性波－波相作用等），并利用该模式对影响杭州湾－长江口沿岸海域的一次台风浪过程进行了模拟研究：模式所需风场由藤田台风风场模型嵌入对应台风特征等压线，并对相应时段的NCAR／NCEPT资料、单站资料进行同化后提供；利用自嵌套的方式提供波谱边界条件；模式模拟的结果与实际海浪观测资料相符较好，在此基础上，研究了底摩擦、深度诱导波破碎、三波相互作用等物理过程联合对近岸台风浪的影响，初步认识了它们在近岸台风浪生成、传播过程中的重要作用。     

The dynamic behaviour of a river-dominated tidal inlet, River Murray, Australia

Australia's largest river, the River Murray, discharges to the southern ocean through a coastal lagoon and river-dominated tidal inlet. Increased water extractions upstream for irrigation have led to significantly reduced flows at the mouth and, as a result, the area is undergoing rapid change, particularly with regard to the rate at which sediment is being transported into the lagoon. Based on detailed and accurate bathymetric surveys it has been possible to estimate that the rate of lagoon in-filling is of the order of 100,000 m³ per year for the period June 2000 to May 2003, although the actual rate shows significant year to year variability. Dredging of the lagoon commenced in 2000 in an attempt to reverse the trend.In an effort to understand the behaviour of the inlet a one-dimensional numerical model of the inlet has been developed. The model extends the original of van de Kreeke by including a dynamic inlet throat area based on predicted river flows and a sediment transport module to predict the resulting net sediment transport. Comparisons with water level data collected on both the ocean and lagoon sides of the mouth have shown that the model is able to predict the attenuation and lag of the tidal signal reasonably well. The sediment transport model was based on predicted sediment concentrations in the surf zone and was found to predict the rate of sediment in-filling to an acceptable level of accuracy. It is envisaged that the model will be a useful management tool, especially since it is possible to manipulate river discharges to the mouth.     

计算域的选取对风暴潮数值模拟的影响

作者以 72 0 3号台风过程中引起的黄海沿岸水文测站的风暴潮过程为例 ,选取不同计算域 ,模拟计算的同一测站的风暴潮增水值有很大差异。只有选取整个黄渤海海域 ,才能得到较精确的风暴潮增水结果。从而揭示在封闭或半封闭海域中由风暴过程激发的区域自由振荡 ,是风暴潮增水中不容忽视的量。说明该海域中的风暴潮过程是海域整体效应的响应 ,因此认为数值模拟中计算域应选取整个封闭或半封闭海域     

Investigation on the Deposition in an Excavated Trough at the East Shore of Jiaozhou Bay, China

- Based on field investigations, this paper analyzes three types of harbour basinns and navigation channel excavated on seabed in Jiaozhou Bay, get a general rule of deposition for excavated trough, it found that pollution is one of crucial factors resulting in the deposition of the excavated trough in the east shore of Jiaozhou Bay. With these results, it predicted the annual deposition thickness for the excavated trough and disclosed the fact that it can't be deposited deadly during one storm. At the same time, with two-dimensional numerical model, it studied the effects of the excavated trough and the reclamation near shore on tidal cureent and said that the excavated trough can decrease the current velocity passing through the trough about 10- 15%, but only limited inside and near the trough and there are no effect on other regions; reclamation can cut off the pollution sources and no obvious effect on the currents of the Jiaozhou Bay. Connecting the deep trough and Cangkou tidal channel with a new