13C示踪技术在利玛原甲藻中腹泻性贝毒合成研究的应用

选取具有显著产毒差异的利玛原甲藻(Prorocentrum lima) SHG株与2XS株为研究对象,以2-¹³C-甘氨酸为示踪原料结合高分辨质谱方法,为腹泻性贝类毒素及其酯化态毒素合成与代谢途径的研究提供新方法和思路。结果表明,两株藻共发现16种腹泻性贝毒及其酯化态毒素成分。对照培养条件下SHG株(大田软海绵酸毒素含量为7.88 pg/cell,鳍藻毒素1含量为4.35 pg/cell)的产毒能力高于2XS株(大田软海绵酸毒素5.70pg/cell),而甘氨酸作为氮源培养条件可显著提高两株藻的产毒总量(P<0.05)。¹³C-甘氨酸标记组与甘氨酸培养组单细胞产毒量无显著变化(P>0.05)。腹泻性贝毒被标记后其同位素异构体丰度发生变化,其中酯化态受¹³C标记的影响程度更高,二级质谱图清晰表示出其碎片离子及脱水峰的标记情况。该方法直观阐明了甘氨酸可作为P. lima标记原料的产毒供体,并使用稳定同位素标记方法首次实现了酯化态的同步标记,有助于腹泻性贝毒生成机制的进一步研究。     

东濮凹陷古近系沙河街组沙三段沉积期湖岸线的变化及岩相古地理特征

东濮凹陷沙三段地层为深水相暗色泥岩与砂岩频繁互层及深水相暗色泥岩与盐膏岩频繁互层.这种砂体是东濮凹陷很重要的含油气储层。过去这种砂体往往按照静态的模式被解释为重力流成因,随着勘探的不断深入,越来越多的证据表明这些砂体中存在着牵引流和暴露地表的标志,其成因和沉积模式需要重新认识。东濮凹陷沙三段沉积时期,湖平面变化频繁,有长周期,也有短周期。根据录井、电性及岩心等资料识别出湖平面变化的5级周期,其变化频率约为1000次/Ma。根据计算,东濮凹陷沙三段沉积时期湖水最大深度为30 m。高水位沉积期,湖岸线距盆地中心较远,盆地沉积中心发育暗色泥岩夹薄层碳酸盐岩,盆地边缘发育粗碎屑岩沉积;水位下降期,边缘相砂体不断向盆地中心进积;低水位期湖岸线向盆地中心退缩了1030 km,盆地沉积中心发育盐膏岩沉积,盐膏岩的周围发育低水位三角洲、扇三角洲和滨湖滩坝相的粗碎屑岩体。正是由于湖平面的变化引起的湖岸线迁移,导致高水位沉积期和低水位期湖盆的岩相古地理面貌发生很大的变化,根据这种动态的模式来预测砂体的分布,在指导油气勘探中产生了明显的效果。     

基于ROMS模式利用集合最优插值同化沿轨海表面高度异常数据的研究

The ensemble optimal interpolation （EnOI） is applied to the regional ocean modeling system （ROMS） with the ability to assimilate the along-track sea level anomaly （TSLA）. This system is tested with an eddy-resolving system of the South China Sea （SCS）. Background errors are derived from a running seasonal ensemble to account for the seasonal variability within the SCS. A fifth-order localization function with a 250 km localization radius is chosen to reduce the negative effects of sampling errors. The data assimilation system is tested from January 2004 to December 2006. The results show that the root mean square deviation （RMSD） of the sea level anomaly decreased from 10.57 to 6.70 cm, which represents a 36.6% reduction of error. The data assimilation reduces error for temperature within the upper 800 m and for salinity within the upper 200 m, although error degrades slightly at deeper depths. Surface currents are in better agreement with trajectories of surface drifters after data assimilation. The variance of sea level improves significantly in terms of both the amplitude and position of the strong and weak variance regions after assimilating TSLA. Results with AGE error （AGE） perform better than no AGE error （NoAGE） when considering the improvements of the temperature and the salinity. Furthermore, reasons for the extremely strong variability in the northern SCS in high resolution models are investigated. The results demonstrate that the strong variability of sea level in the high resolution model is caused by an extremely strong Kuroshio intrusion. Therefore, it is demonstrated that it is necessary to assimilate the TSLA in order to better simulate the SCS with high resolution models.     

Stability Study of a Tide Embankment Subjected to Sea Level Variations Using Centrifugal Model Tests

To investigate the behavior of dredged-sea-sand fill compacted inside tide embankments with a damaged geosynthetic mat, centrifugal model tests and numerical simulation were conducted, both considering variations in sea level. The results from the three centrifugal model tests demonstrate that the subsidence of the dredged-sea-sand fill inside tide embankments with a damaged geosynthetic mat was strongly affected by the loss of dredged-sea-sand into the filter layers with large particles and a decrease in the bearing capacity of the filter layers with small particles. In addition, a comparison of the test and simulation results confirms that the loss of sand into the filter layer and the subsidence of the dredged-sea-sand fill were well reproduced by the numerical simulation.     

A New Methodology for Incorporating Tide Gauge Data in Sea Surface Topography Models

As part of the Vertical Offshore Reference Frames (VORF) project sponsored by the U. K. Hydrographic Office, a new model for Sea Surface Topography (SST) around the British Isles has been developed. For offshore areas (greater than 30 km from the coast), this model is largely derived from satellite altimetry. However, its accuracy and level of detail have been enhanced in coastal areas by the inclusion of not only the 60 PSMSL tide gauges with long-term records around the coasts of the United Kingdom and Ireland but also some 385 gauges established at different epochs and for different observation spans by the U. K. Admiralty. All tide gauge data were brought into a common reference frame by a combination of datum models and direct GPS observations, but a more significant challenge was to bring all short-term sea level observations to an unbiased value at a common epoch. This was achieved through developing a spatial-temporal correlation model for the variations in mean sea level around the British Isles, which in turn meant that gauges with long-term observation spans could be used as control points to improve the accuracy of Admiralty gauges. It is demonstrated that the latter can contribute point observations of mean sea level (MSL) with a precision of 0.078 m. A combination of least squares collocation and interpolation was developed to merge the coastal point and offshore gridded data sets, with particular algorithms having to be developed for different configurations of coastal topology. The resulting model of sea surface topography is shown to present a smooth transition from inshore coastal areas to offshore zones. Further benefits of the techniques developed include an enhanced methodology for detecting datum discontinuities at permanent tide gauges.     

平均大潮高潮面的科学定位和现实描述

在对平均大潮高潮面进行科学定位的基础上,进一步阐明了我国海岸线(岛屿岸线、明礁、灯塔)起算面的定义。引入了三个国标的定义和沿海五省制定的法律条例的规定,结合东海及其岛屿海岸线不同起算面的计算算例,论证了我国海岸线就是平均大潮高潮面形成的实际痕迹线,并以此作为海陆分界线的科学定位和现实意义,澄清了在这一领域存在的一些异议和模糊认识。同时,并就这一重要的基准面进行现实的描述,以期服务于当前我国沿海经济建设、海域使用管理、海岸带开发和有关专项调查以及海洋测绘事业中。     

养殖大黄鱼在自然海区降温不同阶段抗氧化水平及血清酶活性的变化

为了解越冬期间水温下降对大黄鱼抗氧化水平和血清酶活性的影响, 作者研究了养殖大黄鱼(Pseudosciaena crocea)冬季海区自然降温不同阶段(20、16、12、10、8℃)肝脏和肌肉中抗氧化水平及血清酶活性的变化。结果显示: 自然降温过程中, 肝脏中3 种抗氧化酶变化趋势不同, 其中SOD 活性呈升高趋势, 8℃时SOD活性最强; POD 活性呈先升高后降低的变化趋势, 16℃时活性最高; CAT 活性呈下降趋势; 而肌肉中这3 种抗氧化酶活性均呈下降趋势。肝脏中GSH 和MDA 含量在16℃时最高; 肌肉中的3 种抗氧化酶活性、GSH 和MDA 含量以及总抗氧化能力(T-AOC)均明显低于肝脏中, 反映了肝脏对清除自由基具有重要作用, 在抗氧化调节方面起到主要作用。血清酶ALT、AST、ALP、LDH 及CK-MB 随水温自然下降酶活性均呈现降低趋势, 即20℃时酶活性最强; CK 和LIP 随着水温降低, 呈先上升再下降的趋势; ADA 和GGT 随水温降低活性呈上升趋势。血清酶的不同变化, 说明水温对大黄鱼血清酶活性有一定的影响。     

2002-2014年间基于海平面变化的海洋增暖趋势研究

21世纪以来全球变暖进入停滞时期,研究表明,大量热量进入海洋深层是导致全球平均表面温度暂缓上升的主要原因。本文估计和研究了2002.4-2014.12间由热膨胀导致的海平面变化趋势,以此来探测海洋热含量的变化情况。研究使用GRACE重力卫星CSR RL05数据计算了全球海洋的水质量变化,并结合海平面异常数据,计算了由热量变化导致的海平面变化（Net SLA）。将Net SLA与Ishii温度数据计算的海洋热含量进行相关性分析后表明,Net SLA与海洋热含量存在高度相关性,相关系数最大值达0.95。考虑到海洋观测只能表现海洋上层2000m的热含量变化,而除去水质量变化的海平面变化则反映了整层海洋的热含量变化,是估计海洋增暖趋势快慢的有利工具。经计算得出,2002至2014年间南太平洋和南印度洋存在加速增暖趋势,而近年来南半球环状模的增强是导致其增暖的主要原因。     

2004年春季东海赤潮高发区COD分布及其与赤潮关系的初步研究

2004年4～5月初在东海赤潮高发区暴发的特大规模原甲藻赤潮前期和暴发初期对该海域进行的现场调查,并对该海域COD的分布特征进行了探讨。结果表明,赤潮暴发前COD为0．295-1.836mg／L,主要受陆源输入影响。根据其在局部海区底层出现的异常升高结合其他参数分析可对特定海区潜在赤潮暴发的可能性进行评估。赤潮暴发时COD为0．36～3．14mg／L,表层和中层与叶绿素存在显著正相关关系,表明其主要受生物影响。富营养化指数表明赤潮暴发前近一半海域已经处于富营养化状态,但COD对富营养化的贡献不如营养盐重要。     

西沙西科1井元素地球化学特征与海平面升降的关系

以南海北部西沙群岛石岛碳酸盐岩台地为研究对象,对1268件碳酸盐岩样品进行全岩元素地球化学特征分析,并结合早中新世以来沉积学特征,研究其与海平面升降的响应关系。实验结果显示,该地区常量元素（Na2O/K2O、Na2O/SiO2、P2O5）及微量元素（B/Ga、Zr/Al、Li、V）分析的古盐度、古环境氧化还原状态和酸碱度及海平面变化,与岩石地层学和古生物学分析得到的结论基本一致,进而可应用于孤立海相碳酸盐岩台地的沉积层序划分;受限于复杂的白云岩化作用,Mg/Ca难以反映海平面变化。Na2O/K2O、Na2O/SiO2、P2O5 B/Ga、Zr/Al、Li、V等指标表明,早中新世初期三亚组水深逐渐加深,有利于生物礁的发育;中中新世早期梅山组二段海平面第1次达到最高值,礁盘随之扩大,形成较深水环境下的碳酸盐岩台地,以潟湖相和内侧滩相为主;随后水体于中中新世晚期梅山组一段逐渐变浅,持续至晚中新世早期黄流组二段,生物礁受淋滤剥蚀形成礁-滩相沉积;晚中新世晚期黄流组一段海平面再次持续上升,于上新世达到第2次最高值,直至更新世水深才逐渐变浅。